Natural Products in the Prevention of Degenerative Bone and Joint Diseases: Mechanisms Based on the Regulation of Ferroptosis DOI Open Access

Kuanhui Gao,

Lizhi Lv, Zhichao Li

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Degenerative bone and joint diseases (DBJDs), characterized by osteoporosis, osteoarthritis, chronic inflammation of surrounding soft tissues, are systemic conditions primarily affecting the skeletal system. Ferroptosis, a programmed cell death pathway distinct from apoptosis, autophagy, necroptosis. Accumulating evidence suggests that ferroptosis is intricately linked to pathogenesis DBJDs, targeting its regulation could be beneficial in managing these conditions. Natural products, known for their anti-inflammatory antioxidant properties, have shown unique advantages preventing potentially through modulating ferroptosis. This article provides an overview latest research on ferroptosis, with focus role DBJDs therapeutic potential natural products this pathway, offering novel insights prevention treatment DBJDs.

Language: Английский

Novel pH-responsive Lipid Nanoparticles Deliver UA-Mediated Mitophagy and Ferroptosis for Osteoarthritis Treatment DOI Creative Commons

Guoliang Yi,

Min Li, Jiayi Zhou

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101697 - 101697

Published: March 1, 2025

Language: Английский

Citations

0
CircPAFAH1B2 induces chondrocytes mitochondrial dysfunction and promotes cartilage degeneration through binding molecular chaperone ClpB DOI Creative Commons

Yufan Bu,

Chang Zhao,

Yifang Qian

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

This study explores the role of circPAFAH1B2 in osteoarthritis (OA) by investigating its influence on nuclear-mitochondrial communication, a largely unexplored area OA progression. By uncovering how regulates mitochondrial function, aims to identify novel therapeutic targets for prevention and treatment. aimed regulatory communication within chondrocytes cartilage homeostasis. expression was determined via quantitative real-time polymerase chain reaction (qRT-PCR) situ hybridization. RNA pulldown experiments, proteomic analyses, immunoprecipitation were conducted downstream circPAFAH1B2. Gain- loss-of-function assays performed evaluate roles molecular chaperone caseinolytic peptidase B protein homolog (ClpB) function chondrocyte homeostasis cartilage. Cross-linking sequencing binding sites between ClpB. upregulated localized cytoplasm chondrocytes. In vivo vitro experiments demonstrated that increased levels induced dysfunction promoted degeneration. Mechanistic investigations revealed bound restricted import ClpB, which disaggregates misfolded proteins, stabilizes homeostasis, maintains We characterized mutation these effectively suppressed circPAFAH1B2-mediated phenotypes. Our findings indicate acts as decoy blocking ClpB translocation, driving mitochondria-dependent degradation, may provide OA.

Language: Английский

Citations

0
Ferroptosis is involved in the benzene-induced hematotoxicity via mitochondrial ROS-ferritinophagy pathway DOI
Xiaohan Yang,

Shuangyan Dong,

Caihong Xing

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: 376, P. 126379 - 126379

Published: May 5, 2025

Language: Английский

Citations

0
Regulation of ferroptosis in osteoarthritis and osteoarthritic chondrocytes by typical MicroRNAs in chondrocytes DOI Creative Commons
Qingyuan Yu, Yanan Xiao, Mengqi Guan

et al.

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Nov. 5, 2024

Osteoarthritis (OA) is a progressive degenerative disorder impacting bones and joints, worsened by chronic inflammation, immune dysregulation, mechanical stress, metabolic disturbances, various other contributing factors. The complex interplay of cartilage damage, loss, impaired repair mechanisms remains critical formidable aspect OA pathogenesis. At the genetic level, multiple genes have been implicated in modulation chondrocyte metabolism, displaying both promotive inhibitory roles. Recent research has increasingly focused on influence non-coding RNAs regulation distinct cell types within bone tissue OA. In particular, an expanding body evidence highlights regulatory roles microRNAs chondrocytes. This review aims to consolidate most relevant associated with chondrocytes, as identified recent studies, elucidate their involvement processes ferroptosis. Furthermore, this study explores interactions between long (lncRNAs) circular (circRNAs) OA, emphasis microRNA-mediated mechanisms. Finally, gaps current are identified, offering strategic insights advance understanding pathophysiology guide therapeutic developments field.

Language: Английский

Citations

2
Ferroptosis in Osteoarthritis: Current Understanding DOI Creative Commons
Yikai Liu, Zian Zhang,

Fang Yuan

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 8471 - 8486

Published: Nov. 1, 2024

Osteoarthritis (OA) is a prevalent degenerative disease in elderly people that characterized by cartilage loss and abrasion, leading to joint pain dysfunction. The aetiology of OA complicated includes abnormal mechanical stress, mild inflammatory environment, chondrocyte senescence apoptosis, changes metabolism. Ferroptosis regulated cell death modality the excessive accumulation lipid peroxidation mitochondrial role ferroptosis pathogenesis has aroused researchers' attention past two years, there mounting evidence indicating destructive. However, impact on how regulators affect development are unclear. Here, we reviewed current understanding summarized several drugs compounds targeting treatment. intracellular iron, trigger Fenton reaction, production ROS, PUFA-PLs, membrane damage involved ferroptosis. System X

Language: Английский

Citations

2
LINC01232 promotes ARNTL2 transcriptional activation and inhibits ferroptosis of CRC cells through p300/H3K27ac DOI
Shengwei Zhuang,

Zhekun Huang,

Hongkai Fan

et al.

Epigenomics, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 18

Published: Sept. 13, 2024

This study investigated the role of lncRNA

Language: Английский

Citations

1
Identification and validation of novel characteristic genes based on multi-tissue osteoarthritis DOI Creative Commons

Guihao Zheng,

Yulong Ouyang,

Shuilin Chen

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 9, 2024

Abstract Background Osteoarthritis (OA) is characterized by synovial inflammation, articular cartilage degradation, and subchondral bone changes. Currently, there are no reliable biomarkers for the diagnosis treatment of OA. Therefore, exploring OA crucial its prevention, diagnosis, treatment.Materials Methods The GSE51588, GSE12021, GSE55457, GSE56409, GSE114007, GSE168505, GSE169077, GSE55235, GSE129147, GSE48556 datasets patients with normal control samples were obtained from GEO database. Differentially expressed genes (DEGs) in controls identified using R language. Protein-protein interaction (PPI) network module analysis performed to screen filter key genes. Enrichment analyses conducted determine biological functions pathways DEGs predict potential transcription factors. Machine learning models (XGBoost, LASSO regression, SVM) used identify best characteristic genes, intersection hub was as final diagnostic ROC nomogram evaluate value candidate expression levels validated external containing cartilage, membrane, blood patients. gene IRS2 chondrocytes further confirmed through vitro experiments.Results Fifteen (IRS2, ADM, SIK1, PTN, CX3CR1, WNT5A, IL21R, APOD, CRLF1, FKBP5, PNMAL1, NPR3, RARRES1, ASPN, POSTN) three machine algorithms. indicated that abnormal may be mediated extracellular matrix organization, structure Relaxin signaling pathway, IL-17 AGE-RAGE pathway diabetic complications, PI3K-Akt which involved occurrence. Four highly correlated Validation data set showed down-regulated, while POSTN up-regulated experimental group compared group. qRT-PCR WB results verified level consistent bioinformatics results.Conclusion This study integrates algorithms validate four promising biomarkers: IRS2, POSTN. can a biomarker early PTN deserves attention. WNT5A offer new perspectives

Language: Английский

Citations

0
Epigenetic mechanisms of Nsd1-mediated histone methylation modifications in chondrocyte ferroptosis in knee osteoarthritis DOI Creative Commons
Rao Wang, Da Shi,

Xiaoni Pan

et al.

Biomolecules and Biomedicine, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 31, 2024

Knee osteoarthritis (KOA) is a degenerative joint disease characterized by pain, stiffness, and impaired mobility, with current therapies offering limited efficacy. This study investigates the epigenetic role of nuclear receptor-binding SET domain protein 1 (NSD1) in KOA pathogenesis. A mouse model was established, adenoviral vectors were employed to upregulate Nsd1 inhibit SRY-box transcription factor 9 (Sox9), followed histopathological assessments. We examined changes cell morphology, proliferation, viability, ferroptosis-related markers. The expression Nsd1, Sox9, acyl-CoA synthetase long-chain family member 4 (Acsl4) analyzed, along enrichment dimethylated lysine 36 histone 3 (H3K36me2) on Sox9 promoter Acsl4 promoter. Additionally, binding relationship between sequence analyzed. Our results revealed that reduced tissues interleukin (IL)-1β-stimulated chondrocytes. upregulation alleviated KOA, promoted chondrocyte proliferation inhibited ferroptosis. Mechanistically, enhanced H3K36me2 expression, which turn suppressed inhibition partially reversed protective effect overexpression. In summary, mitigates ferroptosis ameliorates modulating downregulate expression.

Language: Английский

Citations

0
Ferroptosis and its role in osteoarthritis: mechanisms, biomarkers, and therapeutic perspectives DOI Creative Commons
Shao‐Chun Lu, Zhenyu Liu,

Mei‐Ling Qi

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 18, 2024

Osteoarthritis (OA) is one of the leading causes disability worldwide, characterized by a complex pathological process involving cartilage degradation, synovial inflammation, and subchondral bone remodeling. In recent years, ferroptosis, form programmed cell death driven iron-dependent lipid peroxidation, has been recognized as playing critical role in onset progression OA. Investigating molecular mechanisms ferroptosis its involvement OA may offer novel strategies for diagnosing treating this disease. This review first outlines core with particular focus on roles molecules such Glutathione Peroxidase 4 (GPX4), Transferrin Receptor 1 (TfR1), Nuclear Coactivator (NCOA4). Subsequently, study examines specific impacts pathophysiology Building this, potential ferroptosis-related biomarkers diagnosis treatment highlighted, along proposed therapeutic targeting regulation. aims to deepen understanding advance clinical application regulatory therapies

Language: Английский

Citations

0
Natural Products in the Prevention of Degenerative Bone and Joint Diseases: Mechanisms Based on the Regulation of Ferroptosis DOI Open Access

Kuanhui Gao,

Lizhi Lv, Zhichao Li

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Degenerative bone and joint diseases (DBJDs), characterized by osteoporosis, osteoarthritis, chronic inflammation of surrounding soft tissues, are systemic conditions primarily affecting the skeletal system. Ferroptosis, a programmed cell death pathway distinct from apoptosis, autophagy, necroptosis. Accumulating evidence suggests that ferroptosis is intricately linked to pathogenesis DBJDs, targeting its regulation could be beneficial in managing these conditions. Natural products, known for their anti-inflammatory antioxidant properties, have shown unique advantages preventing potentially through modulating ferroptosis. This article provides an overview latest research on ferroptosis, with focus role DBJDs therapeutic potential natural products this pathway, offering novel insights prevention treatment DBJDs.

Language: Английский

Citations

0