A gut commensal niche regulates stable association of a multispecies microbiota DOI Open Access
Ren Dodge, Eric W. Jones, Haolong Zhu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: Sept. 30, 2021

Abstract The intestines of animals are typically colonized by a complex, relatively stable microbiota that influences health and fitness, but the underlying mechanisms colonization remain poorly understood. As typical animal, fruit fly, Drosophila melanogaster, is associated with consistent set commensal bacterial species, yet reason for this consistency unknown. Here, we use gnotobiotic flies, microscopy, microbial pulse-chase protocols to show niche exists within proventriculus region foregut selectively binds bacteria exquisite strain-level specificity. Primary colonizers saturate exclude secondary same strain, initial Lactobacillus physically remodels favor Acetobacter . Our results provide mechanistic framework understanding establishment stability an intestinal microbiome. One-Sentence Summary A strain-specific inhabits defined spatial gut forms niche.

Language: Английский

Endosymbionts Reduce Microbiome Diversity and Modify Host Metabolism and Fecundity in the Planthopper Sogatella furcifera DOI Creative Commons
Tong‐Pu Li,

Chun-ying Zhou,

Mengke Wang

et al.

mSystems, Journal Year: 2022, Volume and Issue: 7(2)

Published: March 30, 2022

Endosymbionts can profoundly affect the nutrition, immunity, development, and reproduction of insect hosts, but effects multiple endosymbiont infections on microbiota interaction these with host fitness are not well known. By establishing S. furcifera lines different infection status, we found that Cardinium combined + Wolbachia differentially reduced bacterial diversity as changing community structure affecting metabolism, which may connect to negative endosymbionts their host.

Language: Английский

Citations

25
Construction and Modeling of a Coculture Microplate for Real-Time Measurement of Microbial Interactions DOI Creative Commons
Charles Jo, David B. Bernstein,

Natalie Vaisman

et al.

mSystems, Journal Year: 2023, Volume and Issue: 8(2)

Published: Feb. 21, 2023

The dynamic structures of microbial communities emerge from the complex network interactions between their constituent microorganisms. Quantitative measurements these are important for understanding and engineering ecosystem structure. Here, we present development application BioMe plate, a redesigned microplate device in which pairs wells separated by porous membranes. facilitates measurement integrates easily with standard laboratory equipment. We first applied to recapitulate recently characterized, natural symbiotic bacteria isolated Drosophila melanogaster gut microbiome. Specifically, plate allowed us observe benefit provided two

Language: Английский

Citations

16
Integrative developmental ecology: a review of density-dependent effects on life-history traits and host-microbe interactions in non-social holometabolous insects DOI Creative Commons
Anh The Than, Fleur Ponton, Juliano Morimoto

et al.

Evolutionary Ecology, Journal Year: 2020, Volume and Issue: 34(5), P. 659 - 680

Published: Sept. 2, 2020

Abstract Population density modulates a wide range of eco-evolutionary processes including inter- and intra-specific competition, fitness population dynamics. In holometabolous insects, the larval stage is particularly susceptible to density-dependent effects because larva resource-acquiring stage. Larval can modulate expression life-history traits not only in adult stages but also downstream for dynamics evolution. Better understanding scope generality on current future generations provide useful knowledge both theory experiments developmental ecology. Here, we review literature non-social insects. First, functional definition navigate terminology literature. We then classify biological levels upon which be observed followed by produced over past decades across major groups. Next, argue that host-microbe interactions are yet an overlooked level propose conceptual model explain how curves. summary, this provides integrative framework used guide research field ecology

Language: Английский

Citations

35
Mosquito‐bacteria interactions during larval development trigger metabolic changes with carry‐over effects on adult fitness DOI
Émilie Giraud, Hugo Varet, Rachel Legendre

et al.

Molecular Ecology, Journal Year: 2021, Volume and Issue: 31(5), P. 1444 - 1460

Published: Dec. 14, 2021

Abstract In animals with distinct life stages such as holometabolous insects, adult phenotypic variation is often shaped by the environment of immature stages, including their interactions microbes colonizing larval habitats. Such carry‐over effects were previously observed for several traits mosquito Aedes aegypti after exposure to different bacteria, but mechanistic underpinnings are unknown. Here, we investigated molecular changes triggered gnotobiotic bacteria in Ae . We initially screened a panel 16 bacterial isolates from natural breeding sites determine ability influence life‐history traits. subsequently focused on four (belonging Flavobacterium , Lysobacter Paenibacillus and Enterobacteriaceae) significant survival found that they associated transcriptomic profiles throughout development. Moreover, detected at level gene expression isolates. The most prominent larvae reflected profound remodelling lipid metabolism, which translated into differences storage starvation resistance stage. Together, our findings indicate environmental trigger substantial physiological impact fitness, uncovering possible mechanism underlying mosquito‐bacteria during

Language: Английский

Citations

30
Diet–Microbiota Interactions Alter Mosquito Development DOI Creative Commons
Vincent G. Martinson, Michael R. Strand

Frontiers in Microbiology, Journal Year: 2021, Volume and Issue: 12

Published: June 8, 2021

Gut microbes and diet can both strongly affect the biology of multicellular animals, but it is often difficult to disentangle microbiota-diet interactions due complex microbial communities many animals harbor nutritionally variable diets they consume. While theoretical empirical studies indicate that greater microbiota diversity beneficial for animal hosts, there have been few tests performed in aquatic invertebrates. Most mosquito species are detritivores during their juvenile stages microbiotas consume range from nutrient rich poor. In this study, we produced a gnotobiotic model allowed us examine how between specific gut fitness Aedes aegypti, yellow fever mosquito. Using simplified seven-member community bacteria (ALL7) various laboratory natural diets, larval mosquitoes develop under different dietary conditions measured resulting time adulthood adult size. Larvae inoculated with ALL7 or more developed similarly when fed nutrient-rich rat chow fish food whereas larvae individual bacterial members exhibited differences development large performance diet. contrast, largely failed support growth field-collected detritus unless supplemented additional protein yeast. Collectively, our results contingent on composition.

Language: Английский

Citations

28
Crosstalk between the microbiota and insect postembryonic development DOI Creative Commons
Maxime Girard, Patricia Luis, Claire Valiente Moro

et al.

Trends in Microbiology, Journal Year: 2022, Volume and Issue: 31(2), P. 181 - 196

Published: Sept. 24, 2022

Language: Английский

Citations

21
Investigating local and systemic intestinal signalling in health and disease with Drosophila DOI Creative Commons

Andre Medina,

Karen Bellec, Sofía Polcowñuk

et al.

Disease Models & Mechanisms, Journal Year: 2022, Volume and Issue: 15(3)

Published: March 1, 2022

ABSTRACT Whole-body health relies on complex inter-organ signalling networks that enable organisms to adapt environmental perturbations and changes in tissue homeostasis. The intestine plays a major role as centre by producing local systemic signals are relayed the body maintain intestinal organismal Consequently, disruption of homeostasis associated with diseases multi-organ dysfunction. In recent years, fruit fly Drosophila melanogaster has emerged prime model organism study tissue-intrinsic adult due its genetic tractability functional conservation mammals. this Review, we highlight research contributed our understanding how interacts microenvironment distant organs. We discuss implications these findings for whole-body pathophysiology, future studies might advance knowledge interplay between rest disease.

Language: Английский

Citations

20
Composition and Diversity of Gut Bacterial Community in Different Life Stages of a Leaf Beetle Gastrolina depressa DOI

Meiqi Ma,

Xiaotong Chen,

Siqun Li

et al.

Microbial Ecology, Journal Year: 2022, Volume and Issue: 86(1), P. 590 - 600

Published: June 1, 2022

Language: Английский

Citations

20
The complex interactions between nutrition, immunity and infection in insects DOI Open Access
Fleur Ponton, Yin Xun Tan,

Casey C. Forster

et al.

Journal of Experimental Biology, Journal Year: 2023, Volume and Issue: 226(24)

Published: Dec. 14, 2023

Insects are the most diverse animal group on planet. Their success is reflected by diversity of habitats in which they live. However, these have undergone great changes recent decades; understanding how affect insect health and fitness an important challenge for conservation. In this Review, we focus research that links nutritional environment with infection immune status insects. We first discuss from field immunology, then investigate factors such as intracellular extracellular symbionts, sociality transgenerational effects may interact connection between nutrition immunity. show interactions resistance can be highly specific to species and/or type - almost certainly due social life cycles, varied environments insects Hence, connections cannot easily generalised across finally suggest other environmental aspects use agrochemicals climatic might also influence interaction resistance, highlight essential.

Language: Английский

Citations

11
Microbial changes and associated metabolic responses modify host plant adaptation in Stephanitis nashi DOI
Tong‐Pu Li,

Chen‐Hao Wang,

Jia‐Chu Xie

et al.

Insect Science, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 18, 2024

Abstract Symbiotic microorganisms are essential for the physiological processes of herbivorous pests, including pear lace bug Stephanitis nashi , which is known causing extensive damage to garden plants and fruit trees due its exceptional adaptability diverse host plants. However, specific functional effects microbiome on adaptation S. remains unclear. Here, we identified significant microbial changes in 2 different plants, crabapple cherry blossom, characterized by differences fungal diversity as well bacterial community structures, with abundant correlations between bacteria or fungi. Consistent changes, that fed blossom demonstrated decreased metabolites downregulated key metabolic pathways, such arginine mitogen‐activated protein kinase signaling pathway, were crucial plant adaptation. Furthermore, correlation analysis unveiled numerous differential metabolites, influenced interactions These bacteria, fungi, associated may modify pathways aiding results provide valuable insights into alteration function adapted contributing a better understanding pest invasion dispersal from perspective.

Language: Английский

Citations

4