Analysis Of The Effect Of Knowledge On Entrepreneurship Readiness Using Random Forest Classification Machine Learning DOI Open Access
Ariyono Setiawan

Technium Social Sciences Journal, Journal Year: 2021, Volume and Issue: 23, P. 134 - 149

Published: Sept. 9, 2021

Entrepreneurship is a phenomenon that has an important influence on the progress and welfare of world, so entrepreneurship used as base economic development. Psychologically, entrepreneurs are people who have strong internal drive effort to achieve certain goals they tendency experiment in showing character free from control others. can be seen various points view. The angle context question views several fields, namely according economists, management, business people, psychologists investors. main requirement entrepreneur must entrepreneurial knowledge. readiness determined by knowledge possessed experience conducting (Kurniawati, 2019). In midst rapid development artificial intelligence (AI) technology today. Not many know consists branches, one which machine learning. This learning (ML) branches AI very interesting. sample population this study was obtained air transportation school consisting 7 populations. Data analysis done using . research location with Machine Learning Random Forest Classification cadets, lecturers general public

Language: Английский

Label‐free surface‐enhanced Raman spectroscopy coupled with machine learning algorithms in pathogenic microbial identification: Current trends, challenges, and perspectives DOI Creative Commons
Jia‐Wei Tang, Quan Yuan,

Xin‐Ru Wen

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 2(3)

Published: March 5, 2024

Abstract Infectious diseases caused by microbial pathogens remain a primary contributor to global health burdens. Prompt control and effective prevention of these are critical for public medical diagnostics. Conventional detection methods suffer from high complexity, low sensitivity, poor selectivity. Therefore, developing rapid reliable pathogen has become imperative. Surface‐enhanced Raman Spectroscopy (SERS), as an innovative non‐invasive diagnostic technique, holds significant promise in pathogenic microorganism due its rapid, reliable, cost‐effective advantages. This review comprehensively outlines the fundamental theories (RS) with focus on label‐free SERS strategy, reporting latest advancements technique detecting bacteria, viruses, fungi clinical settings. Furthermore, we emphasize application machine learning algorithms spectral analysis. Finally, challenges faced probed, prospective development is discussed.

Language: Английский

Citations

20

Machine Learning-Assisted Raman Spectroscopy and SERS for Bacterial Pathogen Detection: Clinical, Food Safety, and Environmental Applications DOI Creative Commons
Md Hasan-Ur Rahman,

Rabbi Sikder,

Manoj Tripathi

et al.

Chemosensors, Journal Year: 2024, Volume and Issue: 12(7), P. 140 - 140

Published: July 15, 2024

Detecting pathogenic bacteria and their phenotypes including microbial resistance is crucial for preventing infection, ensuring food safety, promoting environmental protection. Raman spectroscopy offers rapid, seamless, label-free identification, rendering it superior to gold-standard detection techniques such as culture-based assays polymerase chain reactions. However, its practical adoption hindered by issues related weak signals, complex spectra, limited datasets, a lack of adaptability characterization bacterial pathogens. This review focuses on addressing these with recent breakthroughs enabled machine learning (ML), particularly deep methods. Given the regulatory requirements, consumer demand safe products, growing awareness risks pathogens, this study emphasizes pathogen in clinical, settings. Here, we highlight use convolutional neural networks analyzing clinical data surface enhanced sensitizing early rapid pathogens safety potential risks. Deep methods can tackle adequate datasets across diverse samples. We pending future research directions needed accelerating real-world impacts ML-enabled diagnostics accurate diagnosis surveillance critical fields.

Language: Английский

Citations

12

Key steps for improving bacterial SERS signals in complex samples: Separation, recognition, detection, and analysis DOI

Maomei Xie,

Yiting Zhu,

Zhiyao Li

et al.

Talanta, Journal Year: 2023, Volume and Issue: 268, P. 125281 - 125281

Published: Oct. 7, 2023

Language: Английский

Citations

17

Label-Free Identification of Spore-Forming Bacteria Using Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy DOI
Kyosuke Tanaka, Ryosuke Oketani, Takeshi Terada

et al.

The Journal of Physical Chemistry B, Journal Year: 2023, Volume and Issue: 127(9), P. 1940 - 1946

Published: Feb. 23, 2023

Spore-forming bacteria accumulate dipicolinic acid (DPA) to form spores survive in extreme environments. Vibrational spectroscopy is widely used detect DPA and elucidate the existence of bacteria, while vegetative cells, another spore-forming have not been studied extensively. Herein, we applied coherent anti-Stokes Raman scattering (CARS) microscopy spectroscopically identify both cells without staining or molecular tagging. The were identified by strong CARS signals due DPA. Furthermore, observed bright spots image at 1735 cm–1. contained species with C=O bonds because this vibrational mode was associated carbonyl group. One candidate diketopimelic (DKP), a precursor. This hypothesis verified comparing spectrum obtained that DKP analogue (ketopimelic acid) result DFT calculation. results indicate cell sporulation process. spectra can be monitor maturation preformation spores.

Language: Английский

Citations

14

Artificial intelligence-aided rapid and accurate identification of clinical fungal infections by single-cell Raman spectroscopy DOI Creative Commons
Jiabao Xu,

Yanjun Luo,

Jingkai Wang

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: March 22, 2023

Integrating artificial intelligence and new diagnostic platforms into routine clinical microbiology laboratory procedures has grown increasingly intriguing, holding promises of reducing turnaround time cost maximizing efficiency. At least one billion people are suffering from fungal infections, leading to over 1.6 million mortality every year. Despite the increasing demand for diagnosis, current approaches suffer manual bias, long cultivation (from days months), low sensitivity (only 50% produce positive cultures). Delayed inaccurate treatments consequently lead higher hospital costs, mobility rates. Here, we developed single-cell Raman spectroscopy achieve rapid identification infectious fungi. The classification between fungi bacteria infections was initially achieved with 100% specificity using spectra (SCRS). Then, constructed a dataset isolates obtained 94 patients, consisting 115,129 SCRS. By training model an optimized feedback loop, just 5 cells per patient (acquisition 2 s cell) made most accurate classification. This protocol accuracies at species level. transformed assessing samples urinary tract infection, obtaining correct diagnosis raw sample-to-result within 1 h.

Language: Английский

Citations

14

Prospects of single-cell nuclear magnetic resonance spectroscopy with quantum sensors DOI Creative Commons

Nick Ruben Neuling,

Robin D. Allert, Dominik B. Bucher

et al.

Current Opinion in Biotechnology, Journal Year: 2023, Volume and Issue: 83, P. 102975 - 102975

Published: Aug. 11, 2023

Single-cell analysis can unravel functional heterogeneity within cell populations otherwise obscured by ensemble measurements. However, noninvasive techniques that probe chemical entities and their dynamics are still lacking. This challenge could be overcome novel sensors based on nitrogen-vacancy (NV) centers in diamond, which enable nuclear magnetic resonance (NMR) spectroscopy unprecedented sample volumes. In this perspective, we briefly introduce NV-based quantum sensing review the progress made microscale NV-NMR spectroscopy. Last, discuss approaches to enhance sensitivity of NV magnetometers detect biologically relevant concentrations provide a roadmap toward application single-cell analysis.

Language: Английский

Citations

12

Rapid identification and drug resistance screening of respiratory pathogens based on single-cell Raman spectroscopy DOI Creative Commons
Ziyu Liu, Ying Xue, Chun Yang

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 26, 2023

Respiratory infections rank fourth in the global economic burden of disease. Lower respiratory tract are leading cause death low-income countries. The rapid identification pathogens causing lower to help guide use antibiotics can reduce mortality patients with infections. Single-cell Raman spectroscopy is a “whole biological fingerprint” technique that be used identify microbial samples. It has advantages no marking and fast non-destructive testing. In this study, single-cell was collect spectral data six pathogen isolates. T-distributed stochastic neighbor embedding (t-SNE) isolation analysis algorithm compare differences between pathogens. eXtreme Gradient Boosting (XGBoost) establish phenotype database model. classification accuracy isolated samples 93–100%, clinical more than 80%. Combined heavy water labeling technology, drug resistance determined. study showed spectroscopy–D 2 O (SCRS–D O) could rapidly within h.

Language: Английский

Citations

10

Unveiling the efficacy of a bulk Raman spectra-based model in predicting single cell Raman spectra of microorganisms DOI Creative Commons
Thomas J. Tewes, Mario Kerst,

Svyatoslav Pavlov

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(6), P. e27824 - e27824

Published: March 1, 2024

In a previous publication, we trained predictive models based on Raman bulk spectra of microorganisms placed silicon dioxide protected silver mirror slide to make predictions for new spectra, unknown the models, different substrate, namely stainless steel. Now have combined large sections this data and convolutional neural network (CNN) single cell spectra. We show that database microbial material is conditionally suited same species in terms cells. Data 13 (bacteria yeasts) were used. Two could be identified 90% correctly five other 71%–88%. The six remaining predicted by only 0%–49%. Especially stronger fluorescence compared cells but also photodegradation carotenoids are some effects can complicate data. results helpful assessing universal tools or databases.

Language: Английский

Citations

4

Improving Machine Learning–Based Bacterial Discrimination by Learning Single‐Cell Raman Data From Multiple Growth Phases DOI Open Access

Norihiko Oda,

Nanako Kanno, Shingo Kato

et al.

Journal of Raman Spectroscopy, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

ABSTRACT Bacterial discrimination using single‐cell Raman spectroscopy and machine/deep learning techniques has been widely explored for promising applications in medical, environmental, food sciences. To construct a machine‐learning model that can achieve highly accurate robust of bacteria real‐world samples, data consisting spectra bacterial cells acquired under various physiological conditions are essential. Despite much effort to study the effects growth phase on discrimination, it is not yet fully elucidated which phase(s) needs be included training efficiently improve accuracy what phase‐dependent changes cellular components underlie discrimination. Here, we used random forest (RF), an ensemble machine method, discriminate six species, including both Gram‐positive Gram‐negative bacteria, at five different phases ranging from lag late stationary phases. We compared four RF classification models were trained one (either midexponential or stationary), two (midexponential all The species built distinctly exceeded 80% with marked increase 24% 32.5% relative single phase. This was greater than found going (13%). also revealed bands relatively invariant (e.g., proteins) specific DNA/RNA intracellular storage materials) important attaining present provides simple effective way good performance, could extended other such as nutrient, temperature, pH.

Language: Английский

Citations

0

Single-cell pigment analysis of phototrophic and phyllosphere bacteria using simultaneous detection of Raman and autofluorescence spectra DOI Creative Commons
Nanako Kanno, Shinsuke Shigeto

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

ABSTRACT Microbes produce various types of pigments that are essential for their biological activities. Microbial important humans because they used in the food industry and medicine. The visualization evaluation pigment diversity microbial cells living natural environments will contribute not only to understanding ecophysiology but also screening useful microbes. Here, we demonstrate simultaneous, nondestructive detection resonance Raman autofluorescence spectra model purple phototrophic bacteria at single-cell level. measured using confocal laser microspectroscopy with 632.8 nm excitation covered wavenumber range 660–3,022 cm −1 (corresponding 661–783 nm), which from can be detected simultaneously as a baseline. peak position carotenoids provided information on length polyene chain structural characteristics, such conjugated keto groups terminal rings. By contrast, extracted differed pattern depending bacteriochlorophyll type ( or b ), suggesting originates bacteriochlorophyll-related molecules. In addition, revealed leaf surface isolated pigmented could environmental sample. Our study shows fluorescence is tool finding novel microbes uncovering yet unknown relationships between light. IMPORTANCE To understand activities environments, it know biomolecules express situ . this study, report method signatures detect distinguish carotenoid intact, cells. We have shown estimate expression status carotenoid-producing well expressed by surface. requires little pretreatment analyze without destroying cells, making visualizing activity searching unidentified

Language: Английский

Citations

0