Mechanisms and biotechnological applications of transcription factors

Synthetic and Systems Biotechnology, Journal Year: 2023, Volume and Issue: 8(4), P. 565 - 577

Published: Aug. 31, 2023

Transcription factors play an indispensable role in maintaining cellular viability and finely regulating complex internal metabolic networks. These crucial bioactive functions rely on their ability to respond effectors concurrently interact with binding sites. Recent advancements have brought innovative insights into the understanding of transcription factors. In this review, we comprehensively summarize mechanisms by which carry out functions, along calculation experimental-based methods employed identification. Additionally, highlight recent achievements application various biotechnological fields, including cell engineering, human health, biomanufacturing. Finally, current limitations research provide prospects for future investigations are discussed. This review will enlightening theoretical guidance engineering.

Language: Английский

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Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis

Synthetic and Systems Biotechnology, Journal Year: 2023, Volume and Issue: 8(2), P. 281 - 291

Published: March 30, 2023

Bacillus licheniformis is one of the most characteristic Gram-positive bacteria. Its unique genetic background and safety characteristics make it have important biologic applications in food industry, including, biosynthesis high value-added bioproducts, probiotic functions, biological treatment wastes derived from production, etc. In this review, these recent advances are summarized presented systematically for first time. addition, we highlight synthetic biology strategies as a potential driver developing strain wider more efficient application industry. Finally, present current challenges faced provide our perspective on relevant future research directions. summary, review will an illuminating comprehensive that allow in-depth understanding B. promote its effective development

Language: Английский

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Investigation of start-up strategies and temperature regulation on enhanced methanogenesis in anaerobic digestion of food waste

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 498, P. 155198 - 155198

Published: Aug. 26, 2024

Language: Английский

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Lipase Production by Yarrowia lipolytica in Solid-State Fermentation Using Amazon Fruit By-Products and Soybean Meal as Substrate

Catalysts, Journal Year: 2023, Volume and Issue: 13(2), P. 289 - 289

Published: Jan. 27, 2023

The production of polyunsaturated fatty acids from fish oil, which is related to various health benefits including effects against cardiovascular diseases, antihypertensive, anticancer, antioxidant, antidepression, anti-aging, and anti-arthritis effects, among others, can be advantageously performed through the application lipase. However, high cost associated with enzyme make process unfeasible thus alternative substrates should investigated solve these problems. This research aimed produce lipase by Yarrowia lipolytica IMUFRJ50682 in solid-state fermentation using by-products food processing industry (andiroba oil cake soybean meal) verify potential initial hydrolysis further a suitable process. A screening was carried out for analysis andiroba meal combinations different proportions (0:100 100:0, respectively) at 48 h Afterward, solid matrix composed supplemented soy Tween 80 improve activity. enzymatic extract characterized relation protein profile electrophoresis. Finally, biocatalyst produced were applied evaluate an study. Maximum lipolytic activity (63.7 U·g−1) achieved (50:50) after 24 fermentation. Soybean 1.5% (0.001%) emulsion provided increase 1.5-fold (82.52 electrophoretic demonstrated band between 37 40 kDa that may 75 referring α subunit β-conglycinin present meal. After h, showed higher degree (DH) (71.0%) than (61.5%). stable during storage room temperature 7 months. lipases Amazon fruit by-product viable as well industrially suited

Language: Английский

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Microbial valorization of fruit processing waste: opportunities, challenges, and strategies

Current Opinion in Food Science, Journal Year: 2024, Volume and Issue: 56, P. 101147 - 101147

Published: Feb. 22, 2024

Language: Английский

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Regulation mechanism and bioactivity characteristic of surfactin homologues with C14 and C15 fatty acid chains

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: March 27, 2024

Abstract Background Surfactin, a green lipopeptide bio-surfactant, exhibits excellent surface, hemolytic, antibacterial, and emulsifying activities. However, lack of clear understanding the synthesis regulation mechanism surfactin homologue components has hindered customized production products with different biological Results In this study, exogenous valine 2-methylbutyric acid supplementation significantly facilitated C14–C15 proportions (up to 75% or more), positive correlation between proportion fortified concentration. Subsequently, branched-chain amino degradation pathway glutamate are identified as critical pathways in regulating by transcriptome analysis. Overexpression genes bkdAB glnA resulted 1.4-fold 1.3-fold increase C14 surfactin, respectively. Finally, C14-rich was observed enhance emulsification activity, achieving an EI 24 exceeding 60% against hexadecane, while simultaneously reducing hemolytic activity. Conversely, C15-rich demonstrated both antibacterial Conclusion This study presents first evidence potential connection conversion glutamine, providing theoretical basis for targeting structure–activity relationships other compounds. Graphical

Language: Английский

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Ligilactobacillus agilis W70: a probiotic with capacity to enhance ammonia assimilation in the rumen

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 17, 2025

Ruminants excrete both undigested and excess consumed nitrogen (N) through feces urine, which results in the waste of feed protein environmental pollution. This study aims to screen bacteria identify strains with potential improve utilization rumen. In a tube screening test, when 30 mmol/L ammonium sulfate was added as only source liquid medium, among 115 bacterial belonging Bacillus lactic-acid bacteria, 10 highest growth (OD600nm level) comparison other evaluated were identified, these, Ligilactobacillus agilis W70 Limosilactobacillus fermentum M50, had ammonia utilizing capabilities (32.09 40.64%, respectively). A subsequent vitro fermentation experiment conducted diet consisting 0.5 g TMR, 50 mL buffer solution, 25 fresh rumen fluid per serum bottle incubated at 39°C for 24 h. The consisted 3 treatments, CTL, L. (1 × 109 cfu/mL), M50 each treatment 6 replicates, repeated across batches. addition decreased NH3N (p < 0.01), ratio acetate propionate (A:P) increased yields microbial (MCP) 0.01) enzymatic activities glutamate dehydrogenase (GDH) 0.05) glutamine synthetase (GS) 0.05). Further genome analysis revealed that harbors synthetase-encoding genes gdhA glnA play key roles utilization. identified strain exhibits high capability enhances assimilation vitro. further characterized elucidate this potential. studies will be develop new additive efficiency ruminant animals.

Language: Английский

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Biodegradation and mineralization of bisphenol A by a novel soil-derived fungus Paraconiothyrium brasiliense mediated by extracellular laccase

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 488, P. 137460 - 137460

Published: Feb. 1, 2025

Language: Английский

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Biological Cleansing: Probiotic Strategies for Tackling Contaminants in Water and Wastewater

Environmental science and engineering, Journal Year: 2025, Volume and Issue: unknown, P. 255 - 279

Published: Jan. 1, 2025

Language: Английский

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Stable soil moisture promotes shoot performance and shapes the root-rhizosphere microbiome

Agricultural Water Management, Journal Year: 2025, Volume and Issue: 310, P. 109354 - 109354

Published: Feb. 11, 2025

Language: Английский

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