Probiotic Properties and Anti-inflammatory Activity of Bacillus spp. Isolated from Ethnically Fermented Soybean DOI Creative Commons
Welfareson Khongriah, Ruchika Maurya, Kanthi Kiran Kondepudi

et al.

Journal of Pure and Applied Microbiology, Journal Year: 2023, Volume and Issue: 17(4), P. 2525 - 2535

Published: Dec. 1, 2023

Bacillus spp. probiotics used as feed additives can form spores and tolerate the harsh conditions of human digestive system are beneficial for treatment inflammatory bowel syndrome. Since reports on anti-inflammatory properties isolated from fermented food Northeast India have not been explored much. The present study focused BN5, AY5, AN8, possessing these desired properties. In study, isolates were screened their tolerance to acid bile salt, auto-aggregation, hydrophobicity, cholesterol assimilation, antibiotic resistance, antagonistic It was found that possessed desirable probiotic traits. culture supernatant also ability reduce LPS-induced inflammation in murine macrophage (RAW 264.7) cells. All treatments reduced Nitric oxide (NO) production by cell lines. lines analyzed measure level cytokine production. levels TNF-α, IL-6, IL-1β after co-treatment with LPS or supernatant. Results suggested potential candidates

Language: Английский

Bacillus halotolerans SW207 alleviates enterotoxigenic Escherichia coli -induced inflammatory responses in weaned piglets by modulating the intestinal epithelial barrier, the TLR4/MyD88/NF-κB pathway, and intestinal microbiota DOI Creative Commons
Ming‐Han Li,

Dongyu Zhao,

Jialin Guo

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(4)

Published: March 7, 2024

Enterotoxigenic Escherichia coli (ETEC) is one of the major pathogens contributing to piglet diarrhea, with significant implications for both health and economic aspects livestock industry. SW207 an isolate Bacillus halotolerans isolated from cold- disease-resistant Leixiang pigs in Northeastern China. We have discovered that can survive pig's gastrointestinal fluid under conditions high bile salt concentration, displaying potent antagonistic activity against ETEC. In this study, we established a weaned diarrhea model infected ETEC investigate role preventing improving intestinal health. Results indicate upregulates expression tight junction proteins, including claudin-1, occludin, zonula occludens-1, at transcriptional translational levels. Furthermore, reduces serum endotoxin, D-lactic acid, various oxidative stress markers while enhancing mechanical barrier function. terms immune barrier, suppressed activation TLR4/MyD88/NF-κB pathway, reducing inflammatory factors upregulating small intestine mucosal sIgA. Concerning biological significantly content E. intestines promotes abundance beneficial bacteria, thereby mitigating microbiota imbalance caused by summary, has potential prevent ETEC, alleviate inflammation epithelial damage, facilitate changes microbiota. This contributes elucidating mechanisms host-microbe interactions pathogen infections.IMPORTANCEEnterotoxigenic consistently been causing mortality piglets pig farming. The industry traditionally relied on antibiotic administration control ETEC-induced diarrhea. However, overuse antibiotics led emergence drug-resistant zoonotic bacterial pathogens, posing threat public Therefore, there urgent need identify alternatives reduce usage. assessed protective effect novel probiotic analyzed its vivo vitro. study results provide theoretical support reference implementing interventions gut early improve

Language: Английский

Citations

8
Valorisation of waste activated sludge for protease production by Bacillus licheniformis DOI
Juan F. Moreno, Paula Oulego, Sergio Collado

et al.

Journal of Cleaner Production, Journal Year: 2024, Volume and Issue: 445, P. 141282 - 141282

Published: Feb. 17, 2024

Language: Английский

Citations

5
Abridgement of Microbial Esterases and Their Eminent Industrial Endeavors DOI

Fatima Akram,

Taseer Fatima,

Ifrah Shabbir

et al.

Molecular Biotechnology, Journal Year: 2024, Volume and Issue: 67(3), P. 817 - 833

Published: March 9, 2024

Language: Английский

Citations

5
Enzymatic deconstruction of complex biomass of microalgae for organic molecules DOI
Praveen Kumar Ghodke, Aneesh Raj,

Narayana Pillai

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 299 - 324

Published: Jan. 1, 2025

Language: Английский

Citations

0
In vitro evaluation of Bacillus licheniformis protease on the nematodes Panagrellus sp., Meloidogyne incognita, Haemonchus spp. and Trichostrongylus spp DOI
Ana Carolina da Silva, Debora Castro de Souza, Adriane Toledo da Silva

et al.

Next research., Journal Year: 2025, Volume and Issue: 2(2), P. 100308 - 100308

Published: April 7, 2025

Language: Английский

Citations

0
Development of functional consortia for the pretreatment of compostable lignocellulosic waste: A simple and effective solution to a large-scale problem DOI Creative Commons
Juan A. López-González, F. Suárez‐Estrella, M.M. Jurado

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 356, P. 120638 - 120638

Published: March 21, 2024

Microorganisms drive the degradation of organic matter thanks to their enzymatic versatility. However, structure lignocellulose poses a great challenge for microbiota inhabiting compost pile. Our purpose was increase biodegradability vegetable waste in early stages composting process by applying microbial consortium with lignocelllulolytic capacity. For this, previous screening performed among culturable from different processes find inoculants ligninocellulolytic activity. Selected strains were applied as pure culture and consortium. The starting material composed tomato plant pruning remains mixed ratio (50:50 v/v), whose humidity adjusted around 65%. To determine ability both treatments activate biodegradation mixtures, moisture, matter, ash, C/N ratio, 4-day cumulative respirometric index (AT4) rates cellulose, hemicellulose lignin evaluated. Subsequently, real developed which performance compared without inoculum (control). According our tests, three (Bacillus safensis, Bacillus licheniformis Fusarium oxysporum) selected. results showed that application bacteria at low doses (104 CFU g−1 on complete residual pile) resulted higher lignocelullose after 10 days treatment observed fungus or untreated controls. implementation strategy described this work obtaining better agronomic quality than uninoculated Therefore, could be considered an interesting tool bioactivation lignocellulosic prior process.

Language: Английский

Citations

2
Design of a sorbitol-activated nitrogen metabolism-dependent regulatory system for redirection of carbon metabolism flow in Bacillus licheniformis DOI Creative Commons

Hehe He,

Youran Li,

Xufan Ma

et al.

Nucleic Acids Research, Journal Year: 2023, Volume and Issue: 51(21), P. 11952 - 11966

Published: Oct. 18, 2023

Synthetic regulation of metabolic fluxes has emerged as a common strategy to improve the performance microbial cell factories. The present regulatory toolboxes predominantly rely on control and manipulation carbon pathways. Nitrogen is an essential nutrient that plays vital role in growth metabolism. However, availability broadly applicable tools based nitrogen pathways for remains limited. In this work, we novel system harnesses signals associated with metabolism redirect excess flux Bacillus licheniformis. By engineering native transcription factor GlnR incorporating sorbitol-responsive element, achieved remarkable 99% inhibition expression green fluorescent protein reporter gene. Leveraging system, identified optimal redirection point overflow flux, resulting substantial 79.5% reduction acetoin accumulation 2.6-fold increase acetate production. This work highlight significance synthetic biology its valuable contribution engineering. Furthermore, our paves way multidimensional future endeavors.

Language: Английский

Citations

5
Unravelling biochemical and structural features of Bacillus licheniformis GH5 mannanase using site-directed mutagenesis and high-resolution protein crystallography studies DOI
Lorenzo Briganti,

Lívia Regina Manzine,

Caio Cesar de Mello Capetti

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 274, P. 133182 - 133182

Published: June 15, 2024

Language: Английский

Citations

1
Bacillus licheniformisYB06: A Rhizosphere–Genome-Wide Analysis and Plant Growth-Promoting Analysis of a Plant Growth-Promoting Rhizobacterium Isolated from Codonopsis pilosula DOI Creative Commons

Shuo Ni,

Yamiao Wu,

Ning Zhu

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1861 - 1861

Published: Sept. 8, 2024

Codonopsis pilosula, commonly known as Dangshen, is a valuable medicinal plant, but its slow growth and susceptibility to environmental stress pose challenges for cultivation. In pursuit of sustainable agricultural practices enhance the yield quality present study isolated bacterial strain exhibiting plant growth-promoting potential from rhizosphere C. pilosula. This was subsequently identified Bacillus licheniformisYB06. Assessment attributes revealed B. licheniformis YB06 biofertilizer. Whole-genome sequencing genome size 4,226,888 bp with GC content 46.22%, harboring 4325 predicted protein-coding sequences. Genomic analysis diverse array genes linked induced systemic resistance (ISR) (PGP) traits, encompassing phytohormone production, nitrogen assimilation reduction, siderophore biosynthesis, phosphate solubilization, biofilm formation, synthesis PGP-related amino acids, flagellar motility. Seed germination assays demonstrated positive effects on pilosula seedlings. Furthermore, we explored various fertilization regimes, particularly YB06-based biofertilizer, were investigated their impact structure diversity soil community. Our findings that significantly impacted composition diversity, combined application biofertilizer organic fertilizer pronounced enhancement community diversity. represents first report beneficial both microbial These provide theoretical foundation practical guidance development novel bio-organic compound fertilizers, thereby contributing cultivation

Language: Английский

Citations

1
Laccase: A Catalyst for Change in Eco-Friendly Industrial Processes DOI
Igor Carvalho Fontes Sampaio,

Alisson Santos da Silva Quinto,

Isabela Viana Lopes de Moura

et al.

Engineering materials, Journal Year: 2024, Volume and Issue: unknown, P. 371 - 388

Published: Jan. 1, 2024

Language: Английский

Citations

1