Sequential Solid-State and Submerged Fermentation to Increase Yarrowia lipolytica Lipase Production from Palm Oil Production Chain By-Products DOI Creative Commons
Camila P. L. Souza, Adejanildo da S. Pereira, Érika C.G. Aguieiras

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 11(1), P. 3 - 3

Published: Dec. 24, 2024

This study investigates the potential of sequential solid-state and submerged fermentation (SeqF) to enhance lipase production by Yarrowia lipolytica using by-products from palm oil chain. Palm fiber deodorizer distillate (PODD) were utilized as substrates in both stages. Solid-state (SSF) yielded significant activity when was used alone (1.55 U/g 48 h), while (SmF) showed improved enzymatic with combination PODD (1171 U/L 72 h). The integration SSF SmF SeqF achieved superior activities, reaching 4464.5 U/L, an 8.3-fold increase compared alone, Erlenmeyer flasks. SeqF-lyophilized biocatalysts experiments better hydrolytic (131 U/g) best conditions reproduced a 4 L bioreactor (33 U/g). biocatalyst employed esterification reactions synthesize mono- diacylglycerols, achieving 24.3% conversion rate. highlights promising sustainable approach for valorizing agro-industrial residues, contributing advancing circular bioeconomy initiatives.

Language: Английский

Bioengineering in Solid-State Fermentation for next sustainable food bioprocessing DOI Creative Commons
Muyideen Olaitan Bamidele, Micheal Bola Bamikale, Eliseo Cárdenas-Hernández

et al.

Next Sustainability, Journal Year: 2025, Volume and Issue: 6, P. 100105 - 100105

Published: Jan. 1, 2025

Language: Английский

Citations

6
From garbage to treasure: A review on biorefinery of organic solid wastes into valuable biobased products DOI Creative Commons
Adegoke Isiaka Adetunji, Paul J. Oberholster, Mariana Erasmus

et al.

Bioresource Technology Reports, Journal Year: 2023, Volume and Issue: 24, P. 101610 - 101610

Published: Sept. 9, 2023

The incessant dumping of solid waste has been an issue global attention, causing significant risks to the environment and human health. biodegradable organic components materials pose a greater opportunity in production beneficial products. Therefore, adequate management is key mitigate environmental pollution promotes its reuse as feedstock formation valuable Methods such pyrolysis, gasification, etc. are employed for valorization waste. However, these techniques inept treatment due release gaseous pollutants, consumption high energy, among others. Biorefinery green sustainable technology breakdown into biobased products biofertilizer, bioethanol, biohydrogen, bioelectricity, biogas, bioplastics, acids, bioenzymes. This review, therefore, provides understandings on biological technologies useful

Language: Английский

Citations

28
Cell disruption and permeabilization methods for obtaining yeast bioproducts DOI Creative Commons
Gabrielle Victória Gautério, Rhonyele Maciel da Silva, Fellipe Chiara Karraz

et al.

Cleaner Chemical Engineering, Journal Year: 2023, Volume and Issue: 6, P. 100112 - 100112

Published: June 1, 2023

Yeasts are used to produce several bioproducts, including functional bio-molecules, enzymes, biofuels, lipids, pigments, vitamins, organic acids, and other value-added bioproducts. When the production of bioproduct occurs intracellularly, methods disruption traditionally (mechanical non-mechanical), which promote release but also total degradation cell wall with consequent loss yeast viability. As an alternative, permeabilization can be through use external agents (chemical or physical), form pores that increase transfer product membrane, facilitating separation material, increasing metabolites acting as effective way maintaining cellular viability - at least partially. In this review, we summarize advances in compared traditional rupture. We present available for evaluating yeast.

Language: Английский

Citations

25
Temperature and pH Optimization for Protease Production Fermented by Yarrowia lipolytica from Agro-Industrial Waste DOI Creative Commons

Juan López-Trujillo,

Miguel Mellado, Juan A. Ascacio‐Valdés

et al.

Fermentation, Journal Year: 2023, Volume and Issue: 9(9), P. 819 - 819

Published: Sept. 7, 2023

The use of yeasts for the production proteases has increased in demand recent years. Y. lipolytica been reported as a strain with high yields protease production. This work aimed to evaluate impact pH and temperature on using solid-state fermentation (SSF). Soybean, canola meal, cottonseed sesame meal wastes were used nutrient sources at seven levels (4, 5, 6, 7, 8, 9, 10) five temperatures (25, 30, 35, 40, 45 °C). waste source optimal conditions maximum enzyme (EP) obtained by Box–Benhken design. results revealed that 30 °C, 48 h cultivation period, showed best EP 188.75 U/L, followed soybean 117.07 66.71 lowest production, reaching 88.5 U/L up 35 °C. factor exhibited greatest effect biotechnological economic potential enzymes is highlighted.

Language: Английский

Citations

15
The potential, strategies, and challenges of Monascus pigment for food application DOI Creative Commons
Mariana Buranelo Egea, Luciana Arantes Dantas, Tainara Leal de Sousa

et al.

Frontiers in Sustainable Food Systems, Journal Year: 2023, Volume and Issue: 7

Published: March 23, 2023

The demand for dyes from natural sources to substitute synthetic application in the food industry has been continuously increasing due some being associated with several problems, including hypersensitivity, carcinogenesis, and negative environmental impacts. Furthermore, (like pigments) are generally regarded by consumer as safer or fewer side effects—a fact that requires in-depth investigation—, which increases commercial interest such products. In this sense, great focus given biotechnological potential of Monascus sp. produce red, orange, yellow pigments using different types fermentation process (submerged solid-state fermentation), substrates, parameters (temperature, pH, agitation, aeration, etc.), aiming at optimizing reducing costs pigment production. general, shown stability neutral basic elevated temperatures a few hours, metallic ions while not showing acidic many presence light. Applications colorant function foods (candies, bread, yogurt, cheese, beer, meat products) reported improvement color aspect sensory analysis. still seems promising incipient, demonstrating it needs be further studied, mainly concerning vivo systems (inside food) where adverse conditions combined. Regulatory issues heterogeneous around world, creates difficulties expand production commercialization but also demonstrates need studies confirm its safety. mini-review presents potential, strategies, challenges application.

Language: Английский

Citations

13
Lipase Production Through Solid-State Fermentation: Partial Purification and Application for Omega-6 Enrichment in Fish Oil DOI
Aparecida Selsiane Sousa Carvalho, Filipe Smith Buarque, Vanessa Naciuk Castelo‐Branco

et al.

Waste and Biomass Valorization, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 25, 2025

Language: Английский

Citations

0
Critical review on revamping circular economy strategies for the co-production of biosurfactants and lipase from agro-industrial wastes through resource recovery and life cycle assessment DOI

Roshan Jaiswal,

Padmanaban Velayudhaperumal Chellam,

Rangabhashiyam Selvasembian

et al.

Biomass and Bioenergy, Journal Year: 2025, Volume and Issue: 196, P. 107733 - 107733

Published: Feb. 21, 2025

Language: Английский

Citations

0
Surfactant-facilitated metabolic induction enhances lipase production from an optimally formulated waste-derived substrate mix using Aspergillus niger: A case of machine learning modeling and metaheuristic optimization DOI
Andrew Nosakhare Amenaghawon,

Stanley Aimhanesi Eshiemogie,

Nelson Iyore Evbarunegbe

et al.

Bioresource Technology Reports, Journal Year: 2024, Volume and Issue: unknown, P. 101993 - 101993

Published: Nov. 1, 2024

Language: Английский

Citations

2
Evaluation of Yarrowia lipolytica potential for the biodegradation of poly(ethylene terephthalate) (PET) from mooring lines of Oil & Gas offshore platforms DOI Creative Commons
Júlio Cesar Soares Sales, Alanna Botelho, Aparecida Selsiane Sousa Carvalho

et al.

Cleaner Chemical Engineering, Journal Year: 2023, Volume and Issue: 7, P. 100109 - 100109

Published: June 4, 2023

Poly(ethylene terephthalate) (PET) is a widely used polyester with several applications, including offshore platforms and mooring lines of the Oil & Gas industry. Due to widespread use PET growing concern about its environmental impact, biotechnology emerges as potential solution mitigate this problem. This work aims evaluate, for first time, Y. lipolytica IMUFRJ 50682 in biodegradation from waste platforms. In study, by yeast Yarrowia was investigated through submerged cultivation or solid-state fermentation. After fermentation complete, sampled culture medium evidenced an increase transmittance areas related ester group detected Attenuated Total Reflectance Fourier-Transform Infrared Spectroscopy (ATR-FTIR) both strategies all media tested. Besides, crystallinity increased 79% 72% after using CSL-G + DMSO (2% corn steep liquor, 2% glycerol, 5% Dimethyl sulfoxide) fermentation, respectively. Scanning electron microscopy revealed formation scratches erosions on polymer's surface microbial cultivation. The presence monomer, terephthalic acid (TPA) (up 142 µmol L − 1), some intermediates, such bis(2-hydroxyethyl) terephthalate (BHET) mono-(hydroxyethyl) (MHET) growth proves that polymer being degraded. These results suggest good biotechnological depolymerization lines.

Language: Английский

Citations

4
Evaluation of by-products from agricultural, livestock and fishing industries as nutrient source for the production of proteolytic enzymes DOI Creative Commons
Alisson Sisa,

Cristina Sotomayor,

Lucía Buitrón

et al.

Heliyon, Journal Year: 2023, Volume and Issue: 9(10), P. e20735 - e20735

Published: Oct. 1, 2023

This study presents an approach that utilizes low-value agro-industrial by-products as culture media for producing high-value proteolytic enzymes. The objective was to assess the impact of six on production Bacillus subtilis strains, confirmed through comprehensive biochemical, morphological, and molecular analyses, were isolated identified. Enzymatic activity evaluated using azocasein casein substrates, sizes purified extract components determined. results demonstrated bacteria exhibited higher metabolic enzymatic when cultured in containing 1 % soybean oil cake or feather meal. Furthermore, concentrations found hinder protease. Optimal protease synthesis meal achieved after 4 days, both methods. Semi-purification obtained from resulted a significant increase azocaseinolytic caseinolytic activities. Gel electrophoresis analysis revealed multiple bands fractions with highest cake, indicating presence various enzymes varying sizes. These findings highlight potential utilizing efficient sustainable economically viable promising applications industries.

Language: Английский

Citations

4