CABI Agriculture and Bioscience, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 18, 2025
Language: Английский
Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14
Published: May 8, 2023
Plant pathogenic microorganisms cause substantial yield losses in several economically important crops, resulting economic and social adversity. The spread of such plant pathogens the emergence new diseases is facilitated by human practices as monoculture farming global trade. Therefore, early detection identification utmost importance to reduce associated agricultural losses. In this review, techniques that are currently available detect discussed, including culture-based, PCR-based, sequencing-based, immunology-based techniques. Their working principles explained, followed an overview main advantages disadvantages, examples their use pathogen detection. addition more conventional commonly used techniques, we also point some recent evolutions field potential point-of-care devices, biosensors, have gained popularity. These devices can provide fast analysis, easy use, most importantly be for on-site diagnosis, allowing farmers take rapid disease management decisions.
Language: Английский
Citations
82
Bioanalysis, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 10
Published: Feb. 4, 2025
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has transformed molecular biology through its precise gene-editing capabilities. Beyond initial applications in genetic modification, emerged as a powerful tool diagnostics and biosensing. This review explores transition from genome editing to innovative detection methods, including nucleic acid identification, single nucleotide polymorphism (SNP) analysis, protein sensing. Advanced technologies such SHERLOCK DETECTR demonstrate CRISPR's potential for point-of-care diagnostics, enabling rapid highly sensitive detection. The integration of chemical modifications, CRISPR-Chip technology, enzymatic systems like Cas12a Cas13a enhances signal amplification efficiency. These advancements promise decentralized, real-time diagnostic solutions with significant implications global healthcare. Furthermore, the fusion artificial intelligence digital health platforms is paving way more accessible, cost-effective, scalable approaches, ultimately revolutionizing precision medicine.
Language: Английский
Citations
2
ACS Sensors, Journal Year: 2023, Volume and Issue: 8(10), P. 3623 - 3642
Published: Oct. 11, 2023
Over the past few decades, pathogens have posed a threat to human security, and rapid identification of should be one ideal methods prevent major public health security outbreaks. Therefore, there is an urgent need for highly sensitive specific approaches identify quantify pathogens. Clustered Regularly Interspaced Short Palindromic Repeats CRISPR/Cas systems Argonaute (Ago) belong Microbial Defense Systems (MDS). The guided, programmable, targeted activation nucleases by both them leading way new generation detection. We compare these two in terms similarities differences. In addition, we discuss future challenges prospects development biosensors, especially electrochemical biosensors. This review expected afford researchers entering this multidisciplinary field useful guidance provide inspiration more innovative biosensors
Language: Английский
Citations
26
Clinica Chimica Acta, Journal Year: 2024, Volume and Issue: 559, P. 119701 - 119701
Published: May 1, 2024
Language: Английский
Citations
15
Critical Reviews in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25
Published: Sept. 17, 2024
This comprehensive review delves into the advancements and challenges in biosensing, with a strong emphasis on transformative potential of CRISPR technology for early rapid detection infectious diseases. It underscores versatility CRISPR/Cas systems, highlighting their ability to detect both nucleic acids non-nucleic acid targets, seamless integration isothermal amplification techniques. The provides thorough examination latest developments CRISPR-based biosensors, detailing unique properties such as high specificity programmability, which make them particularly effective detecting disease-associated acids. While focuses due its critical role diagnosing diseases, it also explores broader applications thereby acknowledging technology's potential. Additionally, identifies existing challenges, need improved signal real-world applicability, offers future perspectives aimed at overcoming these hurdles. ultimate goal is advance development highly sensitive specific biosensors that can be used widely improving human health, point-of-care settings resource-limited environments.
Language: Английский
Citations
14
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: July 13, 2024
Many CRISPR-Cas immune systems generate guide (g)RNAs using trans-activating CRISPR RNAs (tracrRNAs). Recent work revealed that Cas9 tracrRNAs could be reprogrammed to convert any RNA-of-interest into a gRNA, linking the RNA's presence Cas9-mediated cleavage of double-stranded (ds)DNA. Here, we reprogram from diverse Cas12 nucleases, an dsDNA and subsequent collateral single-stranded DNA cleavage-all without RNA necessarily encoding protospacer-adjacent motif (PAM). After elucidating nuclease-specific design rules, demonstrate PAM-independent detection with Cas12b, Cas12e, Cas12f nucleases. Furthermore, rationally truncating target boosts activity, while absence gRNA reduces background activity enhances sensitivity. Finally, apply this platform detect 16 S rRNA sequences five different bacterial pathogens universal tracrRNA. These findings extend tracrRNA reprogramming dsDNA-targeting expanding flexibility versatility CRISPR-based detection.
Language: Английский
Citations
10
TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 166, P. 117198 - 117198
Published: July 24, 2023
Language: Английский
Citations
22
Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 405, P. 135296 - 135296
Published: Jan. 10, 2024
Language: Английский
Citations
6
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3271 - 3271
Published: March 13, 2024
The advent of CRISPR/Cas9 technology has revolutionized genome editing, enabling the attainment once-unimaginable goals. CRISPR/Cas's groundbreaking attributes lie in its simplicity, versatility, universality, and independence from customized DNA-protein systems, erasing need for specialized expertise broadening scope applications. It is therefore more used modification including generation mutants. Beyond such editing scopes, recent development novel or modified Cas-based systems spawned an array additional biotechnological tools, empowering both fundamental applied research. Precisely targeting DNA RNA sequences, CRISPR/Cas system been harnessed fields as diverse gene regulation, deepening insights into expression, epigenetic changes, spatial organization, chromatin dynamics. Furthermore, it aids imaging sequencing, well effective identification countering viral pathogens plants animals. All all, non-editing aspect exhibits tremendous potential across domains, diagnostics, biotechnology, This article reviews critically evaluates primary CRISPR/Cas-based tools developed animals, underlining their transformative impact.
Language: Английский
Citations
6
Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(31), P. 11765 - 11788
Published: July 28, 2023
Pests and disease-causing pathogens frequently impede agricultural production. An early efficient diagnostic tool is crucial for effective disease management. Clustered regularly interspaced short palindromic repeats (CRISPR) the CRISPR-associated protein (Cas) have recently been harnessed to develop tools. The CRISPR/Cas system, composed of Cas endonuclease guide RNA, enables precise identification cleavage target nucleic acids. inherent sensitivity, high specificity, rapid assay time system make it an alternative diagnosing plant identifying genetically modified crops. Furthermore, its potential multiplexing suitability point-of-care testing at field level provide advantages over traditional systems such as RT-PCR, LAMP, NGS. In this review, we discuss recent developments in based diagnostics their implications various applications. We also emphasized major challenges with possible solutions provided insights into future perspectives applications agriculture.
Language: Английский
Citations
16