Species-level verification of Phascolarctobacterium association with colorectal cancer DOI Creative Commons
Cecilie Bucher-Johannessen, Thulasika Senthakumaran, Ekaterina Avershina

et al.

mSystems, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

ABSTRACT We have previously demonstrated an association between increased abundance of Phascolarctobacterium and colorectal cancer (CRC) adenomas in two independent Norwegian cohorts. Here we seek to verify our previous findings using new cohorts methods. In addition, characterize lifestyle sex specificity, the functional potential species, their interaction with other microbial species. analyze 16S rRNA sequencing, shotgun metagenome species-specific qPCR, 2350 samples from three cohorts—CRCAhus, NORCCAP, CRCbiome—and a large publicly available data set, curatedMetagenomicData. Using metagenome-assembled genomes CRCbiome study, explore genomic characteristics pangenome. Three species associated adenoma/CRC were consistently detected by qPCR sequencing. Positive associations adenomas/CRC verified for succinatutens negative shown faecium adenoma Men show higher prevalence P. across Co-occurrence among was low (<6%). Each shows distinct composition forms correlation networks bacterial taxa, although Dialister invisus negatively correlated all investigated Pangenome analyses showed be enriched genes related porphyrin metabolism degradation complex carbohydrates, whereas glycoside hydrolase enzyme 3 specific . IMPORTANCE Until now has been going under radar as CRC-associated genus despite having noted, but overseen, such over decade. found not just one, CRC— more abundant adenoma/CRC, while less adenoma. them represents communities, constituted partners metabolic capacities—and they rarely occur together same patients. that is CRC this should recognized most important bacteria.

Language: Английский

Plasma Metabolites and Life’s Simple 7 in REGARDS DOI
Naruchorn Kijpaisalratana, Zsuzsanna Ament, Amit Patki

et al.

Stroke, Journal Year: 2024, Volume and Issue: 55(5), P. 1191 - 1199

Published: March 14, 2024

The American Heart Association's Life's Simple 7 (LS7) is a health metric that captures important factors associated with cardiovascular and cerebrovascular health. Previous studies highlight the potential of plasma metabolites to serve as marker for lifestyle behavior could be target stroke prevention. objectives this study were identify LS7 incident ischemic mediate relationship between two.

Language: Английский

Citations

3
Sialylation of dietary mucin modulate its digestibility and the gut microbiota of elderly individuals DOI
Fang Su, Jianrong Wu,

Wenxuan Niu

et al.

Food Research International, Journal Year: 2024, Volume and Issue: 184, P. 114246 - 114246

Published: March 19, 2024

Language: Английский

Citations

3
The immune interactions of gut glycans and microbiota in health and disease DOI
Mahmut Demirturk, Mukaddes Sena Cinar, Fikri Y. Avci

et al.

Molecular Microbiology, Journal Year: 2024, Volume and Issue: 122(3), P. 313 - 330

Published: May 4, 2024

Abstract The human digestive system harbors a vast diversity of commensal bacteria and maintains symbiotic relationship with them. However, imbalances in the gut microbiota accompany various diseases, such as inflammatory bowel diseases (IBDs) colorectal cancers (CRCs), which significantly impact well‐being populations globally. Glycosylation mucus layer is crucial factor that plays critical role maintaining homeostatic environment gut. This review delves into how microbiota, immune cells, work together to establish balanced environment. Specifically, glycosylation regulating cell responses metabolism this process examined.

Language: Английский

Citations

3
Sialic acids in infection and their potential use in detection and protection against pathogens DOI Creative Commons
Simone Dedola, Sanaz Ahmadipour, Peterson de Andrade

et al.

RSC Chemical Biology, Journal Year: 2023, Volume and Issue: 5(3), P. 167 - 188

Published: Dec. 19, 2023

An overview of sialic acid involvement in infection and exploitation diagnostics therapeutics.

Language: Английский

Citations

9
Gut Microbial Sialidases and Their Role in the Metabolism of Human Milk Sialylated Glycans DOI Open Access

Diego Muñoz-Provencio,

Marı́a J. Yebra

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(12), P. 9994 - 9994

Published: June 10, 2023

Sialic acids (SAs) are α-keto-acid sugars with a nine-carbon backbone present at the non-reducing end of human milk oligosaccharides and glycan moiety glycoconjugates. SAs displayed on cell surfaces participate in regulation many physiologically important cellular molecular processes, including signaling adhesion. Additionally, sialyl-oligosaccharides from act as prebiotics colon by promoting settling proliferation specific bacteria SA metabolism capabilities. Sialidases glycosyl hydrolases that release α-2,3-, α-2,6- α-2,8-glycosidic linkages terminal residues oligosaccharides, glycoproteins glycolipids. The research sialidases has been traditionally focused pathogenic microorganisms, where these enzymes considered virulence factors. There is now growing interest commensal probiotic their potential transglycosylation activity for production functional mimics to complement infant formulas. This review provides an overview exo-alpha-sialidases gastrointestinal tract some insights into biological role biotechnological applications.

Language: Английский

Citations

8
Changes in Bacterial Gut Composition in Parkinson’s Disease and Their Metabolic Contribution to Disease Development: A Gut Community Reconstruction Approach DOI Creative Commons

Johanna Forero-Rodríguez,

Johannes Zimmermann, Jan Taubenheim

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(2), P. 325 - 325

Published: Feb. 4, 2024

Parkinson’s disease (PD) is a chronic and progressive neurodegenerative with the major symptoms comprising loss of movement coordination (motor dysfunction) non-motor dysfunction, including gastrointestinal symptoms. Alterations in gut microbiota composition have been reported PD patients vs. controls. However, it still unclear how these compositional changes contribute to etiology progression. Furthermore, most available studies focused on European, Asian, North American cohorts, but microbiomes Latin America not characterized. To address this problem, we obtained fecal samples from Colombian participants (n = 25 controls, n idiopathic cases) characterize taxonomical community during via 16S rRNA gene sequencing. An analysis differential composition, diversity, personalized computational modeling was carried out, given bacterial diet each participant. We found three metabolites that differed dietary habits between controls: carbohydrates, trans fatty acids, potassium. identified six genera changed significantly their relative abundance belonging families Lachnospiraceae, Lactobacillaceae, Verrucomicrobioaceae, Peptostreptococcaceae, Streptococcaceae. metabolic microbiome revealed predicted production seven (Indole, tryptophan, fructose, phenylacetic acid, myristic 3-Methyl-2-oxovaleric N-Acetylneuraminic acid). These are associated metabolism aromatic amino acids consumption diet. Therefore, research suggests individual’s intestinal could affect host metabolism. findings open door study microbiome–host interactions allow us medicine.

Language: Английский

Citations

2
N‐glycolylneuraminic acid in red meat and processed meat is a health concern: A review on the formation, health risk, and reduction DOI

Meilian Liang,

Jianping Wu, Hongying Li

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(2)

Published: Feb. 23, 2024

Abstract One of the most consistent epidemiological associations between diet and human disease risk is impact consuming red meat processed products. In recent years, health concerns surrounding have gained worldwide attention. The fact that humans lost ability to synthesize N ‐glycolylneuraminic acid (Neu5Gc) makes products important source exogenous Neu5Gc for humans. As our research has increased, it been discovered in a key factor many major diseases. Given objective evidence harmful caused by health, there need heightened attention field food. This updated review several aspects given including biosynthetic pathway its accumulation body, distribution food, methods detecting Neu5Gc, importantly, systematic existing reducing content meat. It also provides some insights into current status future directions this area.

Language: Английский

Citations

2
Revisiting the immunopathology of congenital disorders of glycosylation: an updated review DOI Creative Commons
Carlota Pascoal, Rita Francisco,

Patrícia Mexia

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 14, 2024

Glycosylation is a critical post-translational modification that plays pivotal role in several biological processes, such as the immune response. Alterations glycosylation can modulate course of various pathologies, case congenital disorders (CDG), group more than 160 rare and complex genetic diseases. Although link between dysfunction has already been recognized, involvement most CDG remains largely unexplored poorly understood. In this study, we provide an update on clinical manifestations 12 with major involvement, organized into 6 categories inborn errors immunity according to International Union Immunological Societies (IUIS). The phosphomannomutase 2 (PMM2)-CDG - frequent was comprehensively reviewed, highlighting higher prevalence issues during infancy childhood R141H-bearing genotypes. Finally, using PMM2-CDG model, point links abnormal patterns host cells possibly favored interactions microorganisms may explain susceptibility infection. Further characterizing immunopathology unusual host-pathogen adhesion not only improve immunological standards care but also pave way for innovative preventive measures targeted glycan-based therapies quality life people living CDG.

Language: Английский

Citations

2
Molecular determinants of Neu5Ac binding to tripartite ATP independent periplasmic (TRAP) transporter DOI
Parveen Goyal, KanagaVijayan Dhanabalan, Mariafrancesca Scalise

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 30, 2024

Abstract N -Acetylneuraminic acid (Neu5Ac) is a negatively charged nine-carbon amino-sugar that often the peripheral sugar in human cell-surface glycoconjugates. Some bacteria scavenge, import, and metabolize Neu5Ac, or they redeploy it on their cell surfaces for immune evasion. The import of Neu5Ac by many mediated tripartite ATP-independent periplasmic (TRAP) transporters. We have previously reported structures SiaQM, membrane-embedded component Haemophilus influenzae TRAP transport system (Currie, M J, et. al 2024). However, published do not contain bound to SiaQM. This information critical defining mechanism further structure-activity relationship studies. Here, we report structure Fusobacterium nucleatum SiaQM with without binding. Both are an inward (cytoplasmic side) facing conformation. Neu5Ac-bound reveals interactions transporter its Na + binding sites. Two -binding sites similar those described previously. discover presence third metal-binding site away buried elevator domain. Ser300 Ser345 interact C1-carboxylate group Neu5Ac. Proteoliposome-based assays showed Ser300-Neu5Ac interaction transport, whereas dispensable. primarily interacts residues domain protein, thereby supporting operator mechanism. interacting conserved, providing fundamental required design inhibitors against this class proteins.

Language: Английский

Citations

2
Changes in the Serum and Tissue Levels of Free and Conjugated Sialic Acids, Neu5Ac, Neu5Gc, and KDN in Mice after the Oral Administration of Edible Bird’s Nests: An LC–MS/MS Quantitative Analysis DOI Creative Commons

Meng-Hua Wang,

Zhifan Wang,

Man Yuan

et al.

Separations, Journal Year: 2024, Volume and Issue: 11(4), P. 107 - 107

Published: April 1, 2024

Edible bird’s nests have a variety of biological activities, the main components which are sialic acids. Sialic acids group nine-carbon N-acetylated derivatives neuraminic acid containing keto at position C2 and play important roles in many processes. To verify whether oral administration edible would change content distribution vivo, liquid chromatography–mass spectrometry method for quantitative analysis levels serum tissues was developed. In negative ion mode, mobile phases consist 0.1% formic water (A) acetonitrile (v/v) (B). Isocratic elution performed with 60% B 0−15 min. The chromatographic separation on Morphling HILIC Amide column (2.1 mm × 150 mm, 5 μm) flow rate 0.5 mL min−1. results showed that correlation coefficients typical calibration curves were all higher than 0.995, exhibiting good linearity. free conjugated forms N-glycolylneuraminic (Neu5Gc), N-acetylneuraminic (Neu5Ac), 2-keto-3-deoxy-D-glycero-D-galactonononic (KDN) different simultaneously detected after daily dose 300 700 mg Kg−1 seven days mice. Our study found can significantly increase concentration total (Neu5Gc + Neu5Ac KDN) spleen lungs tissues, may be related to anti-inflammatory immune function nest, but further studies needed this. dominant brain tissue, Neu5Gc other including heart, liver, spleen, lungs, kidney. Moreover, we mainly groups except liver tissue. conclusion, established had linearity accuracy; it allowed analytes effectively separated from matrix endogenous substances or so could detect beneficial research exploitation

Language: Английский

Citations

2