Microbiological Research, Journal Year: 2024, Volume and Issue: 284, P. 127728 - 127728
Published: April 17, 2024
Language: Английский
Citations
11
Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108326 - 108326
Published: Feb. 1, 2025
Hypoxia and ischemia damage sensitive organelles such as mitochondria, thus mitochondrial dysfunction contributes to metabolic disorders in crustaceans under hypoxia. The mechanisms associated with ferroptosis hypoxic have not been determined crustaceans. In particular, the early molecular events of dynamics require clarification. this study, two evolutionarily conserved fission proteins, Drp1 MTP18, were identified oriental river prawn (Macrobrachium nipponense). vitro, ferroptosis-mediated impairment membrane potential was induced by hypoxia hemocytes. hypoxia-induced hemocytes, activation increased phosphorylation at S616 identified. translocation also increased, fusion-related protein expression decreased vivo. Altered fission-fusion linked dysfunction, inducing a classic mechanism. Marf overexpression or knockdown protected against ferroptotic cell death vitro. Furthermore, verified be driven Drp1/MTP18 interaction. Under hypoxia, MTP18 transcription binding activated HIF-1α response elements (HREs) its promoter. Conjointly, resulted less apoptosis mortality gill tissue vitro; suggesting that adaptation involves vital function MTP18. conclusion, we uncovered role death. Therefore, suggest specific modulation MTP18/DRP1-mediated might therapeutic strategy stress-induced injury invertebrates.
Language: Английский
Citations
1
Micromachines, Journal Year: 2025, Volume and Issue: 16(2), P. 126 - 126
Published: Jan. 23, 2025
Hematological analysis is crucial for diagnosing and monitoring blood-related disorders. Nevertheless, conventional hematology analyzers remain confined to laboratory settings due their high cost, substantial space requirements, maintenance needs. Herein, we present a portable cell tracking velocimetry (CTV) device the precise measurement of magnetic susceptibility biological entities at single-cell level, focusing on red blood cells (RBCs) in this work. The system integrates microfluidic channel positioned between permanent magnets that generate well-defined field gradient (191.82 TA/mm2). When are injected into chamber, particular response recorded used estimate properties quantify intracellular hemoglobin (Hb) concentration. We successfully track over 400 RBCs per condition using imaging trajectory analysis, enabling detailed characterizations physical properties. A comparison mean corpuscular measurements revealed strong correlation our CTV standard ultraviolet-visible (UV-Vis) spectrophotometry (23.1 ± 5.8 pg vs. 22.4 3.9 pg, p > 0.05), validating accuracy measurements. system's resolution reveals population distributions unobtainable through bulk methods. Thus, technology provides rapid, label-free approach characterization, offering new possibilities point-of-care hematological field-based research applications.
Language: Английский
Citations
0
Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742289 - 742289
Published: Feb. 1, 2025
Language: Английский
Citations
0
Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown
Published: March 18, 2025
Language: Английский
Citations
0
ACS Materials Letters, Journal Year: 2024, Volume and Issue: 6(9), P. 4209 - 4229
Published: Aug. 19, 2024
Programmed cell death (PCD) is crucial for renewal, embryogenesis, the immune response, tissue growth regulation, and other essential biological processes. Recent evidence underscores potential of harnessing PCD to combat bacterial infections, particularly in eradicating antibiotic-resistant superbugs. Extensive efforts have been devoted developing PCD-mediated anti-infective agents by drawing insights from materials science, chemistry, immunology, microbiology. In this review, challenges addressing infections PCD-based approaches revolutionize treatment are first summarized discussed. Then, a comprehensive examination nanoantibacterial therapy, encompassing various pathways, such as apoptosis, ferroptosis, cuproptosis, immunogenic death, NETosis, autophagy, pyroptosis, provided. Finally, barriers prospects PCD-driven antimicrobial strategies explored.
Language: Английский
Citations
3
Reviews in Aquaculture, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 11, 2024
ABSTRACT Cell death mechanisms in crustaceans are a complex interplay of processes essential for maintaining cellular homeostasis and immune defense. Modes cell like apoptosis, necroptosis, necrosis well‐documented crustaceans, serving crucial roles removing damaged or infected cells. Unlike other organisms, likely lack pyroptosis, type programmed associated with innate immunity inflammation, because they do not possess the gasdermin genes this process. Recently, NETosis ferroptosis have emerged as significant pathogen NETosis, involving release DNA fibers antimicrobial proteins, helps trap neutralize pathogens, while ferroptosis, an iron‐dependent form death, contributes to lipid peroxidation responses. Cuproptosis, although yet studied context crustacean immunity, shows potential crosstalk particularly regulation metal ion homeostasis, oxidative stress, metabolism. Understanding these offers promising applications aquaculture, such developing targeted modulators enhancing disease resistance economically important species.
Language: Английский
Citations
3
Aquaculture Reports, Journal Year: 2025, Volume and Issue: 42, P. 102856 - 102856
Published: May 6, 2025
Language: Английский
Citations
0
Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 196, P. 106928 - 106928
Published: Sept. 11, 2024
Language: Английский
Citations
1
Aquaculture, Journal Year: 2024, Volume and Issue: unknown, P. 741766 - 741766
Published: Oct. 1, 2024
Language: Английский
Citations
0