An Update on Adipose‐Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity

Advanced Science, Journal Year: 2023, Volume and Issue: 10(20)

Published: May 10, 2023

Abstract Over the last decade, adipose‐derived stem cells (ADSCs) have attracted increasing attention in field of regenerative medicine. ADSCs appear to be most advantageous cell type for therapies owing their easy accessibility, multipotency, and active paracrine activity. This review highlights current challenges translating ADSC‐based into clinical settings discusses novel strategies overcome limitations ADSCs. To further establish as an emerging platform medicine, this also provides update on advancements field, including fat grafting, wound healing, bone regeneration, skeletal muscle repair, tendon reconstruction, cartilage cardiac nerve regeneration. are expected more tissue‐specific increasingly important

Language: Английский

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Apoptotic bodies: bioactive treasure left behind by the dying cells with robust diagnostic and therapeutic application potentials

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: July 12, 2023

Abstract Apoptosis, a form of programmed cell death, is essential for growth and tissue homeostasis. Apoptotic bodies (ApoBDs) are extracellular vesicles (EVs) released by dying cells in the last stage apoptosis were previously regarded as debris dead cells. Recent studies unraveled that ApoBDs not but bioactive treasure left behind with an important role intercellular communications related to human health various diseases. Defective clearance infected-cells-derived possible etiology some Therefore, it necessary explore function mechanism action different physiological pathological conditions. advances have elucidated immunomodulatory, virus removal, vascular protection, regenerative, disease diagnostic potential ApoBDs. Moreover, can be used drug carriers enhancing stability, cellular uptake, targeted therapy efficacy. These reports from literature indicate hold promising diagnosis, prognosis, treatment diseases, including cancer, systemic inflammatory cardiovascular regeneration. This review summarizes recent ApoBDs-related research discusses diseases well challenges prospects ApoBDs-based therapeutic applications. Graphical

Language: Английский

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Apoptotic Bodies Derived from Fibroblast‐Like Cells in Subcutaneous Connective Tissue Inhibit Ferroptosis in Ischaemic Flaps via the miR‐339‐5p/KEAP1/Nrf2 Axis

Advanced Science, Journal Year: 2024, Volume and Issue: 11(24)

Published: April 19, 2024

Abstract Preventing and treating avascular necrosis at the distal end of flaps are critical to surgery success, but current treatments not ideal. A recent study shows that apoptotic bodies (ABs) generated near site apoptosis can be taken up promote cell proliferation. The reveals ABs derived from fibroblast‐like cells in subcutaneous connective tissue (FSCT cells) skin promoted ischaemic flap survival. It is also found inhibited death oxidative stress M1‐to‐M2 polarization macrophages. Transcriptome sequencing protein level testing demonstrated survival endothelial macrophages by inhibiting ferroptosis via KEAP1‐Nrf2 axis. Furthermore, microRNA (miR) data vitro vivo experiments KEAP1 delivering miR‐339‐5p exert therapeutic effects. In conclusion, FSCT cell‐derived ferroptosis, macrophage transition miR‐339‐5p/KEAP1/Nrf2 axis These results provide a potential strategy administering ABs.

Language: Английский

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The uptake of extracellular vesicles: research progress in cancer drug resistance and beyond

Drug Resistance Updates, Journal Year: 2025, Volume and Issue: 79, P. 101209 - 101209

Published: Feb. 1, 2025

Language: Английский

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Exosomes as Therapeutic and Diagnostic Tools: Advances, Challenges, and Future Directions

Cell Biochemistry and Biophysics, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Language: Английский

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Exosomal cargos-mediated metabolic reprogramming in tumor microenvironment

Journal of Experimental & Clinical Cancer Research, Journal Year: 2023, Volume and Issue: 42(1)

Published: March 10, 2023

Metabolic reprogramming is one of the hallmarks cancer. As nutrients are scarce in tumor microenvironment (TME), cells adopt multiple metabolic adaptations to meet their growth requirements. not only present cells, but exosomal cargos mediates intercellular communication between and non-tumor TME, inducing remodeling create an outpost microvascular enrichment immune escape. Here, we highlight composition characteristics meanwhile summarize components corresponding sorting mode. Functionally, these cargos-mediated improves "soil" for metastasis. Moreover, discuss abnormal metabolism targeted by its potential antitumor therapy. In conclusion, this review updates current role TME enriches future application scenarios exosomes.

Language: Английский

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Tailored Apoptotic Vesicle Delivery Platform for Inflammatory Regulation and Tissue Repair to Ameliorate Ischemic Stroke

ACS Nano, Journal Year: 2023, Volume and Issue: 17(9), P. 8646 - 8662

Published: April 26, 2023

Apoptotic vesicles (ApoVs) hold great promise for inflammatory regulation and tissue repair. However, little effort has been dedicated to developing ApoV-based drug delivery platforms, while the insufficient targeting capability of ApoVs also limits their clinical applications. This work presents a platform architecture that integrates apoptosis induction, loading, functionalized proteome regulation, followed by modification, enabling creation an apoptotic vesicle system treat ischemic stroke. Briefly, α-mangostin (α-M) was utilized induce mesenchymal stem cell (MSC) being loaded onto MSC-derived as anti-oxidant anti-inflammatory agent cerebral ischemia/reperfusion injury. Matrix metalloproteinase activatable cell-penetrating peptide (MAP), microenvironment-responsive peptide, modified on surface obtain MAP-functionalized α-M-loaded ApoVs. Such engineered targeted injured brain after systemic injection achieved enhanced neuroprotective activity due synergistic effect α-M. The internal protein payloads ApoVs, upon α-M activation, were found engaged in regulating immunological response, angiogenesis, proliferation, all which contributed therapeutic effects findings provide universal framework creating systems amelioration diseases demonstrate potential neural

Language: Английский

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MSC-EVs alleviate osteoarthritis by regulating microenvironmental cells in the articular cavity and maintaining cartilage matrix homeostasis

Ageing Research Reviews, Journal Year: 2023, Volume and Issue: 85, P. 101864 - 101864

Published: Jan. 25, 2023

Language: Английский

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“Find-eat” strategy targeting endothelial cells via receptor functionalized apoptotic body nanovesicle

Science Bulletin, Journal Year: 2023, Volume and Issue: 68(8), P. 826 - 837

Published: March 21, 2023

Language: Английский

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Extracellular vesicles: emerging roles, biomarkers and therapeutic strategies in fibrotic diseases

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: May 24, 2023

Abstract Extracellular vesicles (EVs), a cluster of cell-secreted lipid bilayer nanoscale particles, universally exist in body fluids, as well cell and tissue culture supernatants. Over the past years, increasing attention have been paid to important role EVs effective intercellular communicators fibrotic diseases. Notably, EV cargos, including proteins, lipids, nucleic acids, metabolites, are reported be disease-specific can even contribute fibrosis pathology. Thus, considered biomarkers for disease diagnosis prognosis. Emerging evidence shows that derived from stem/progenitor cells great prospects cell-free therapy various preclinical models diseases engineered improve targeting effectiveness their treatment. In this review, we will focus on biological functions mechanisms diseases, potential novel therapeutic strategies.

Language: Английский

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