Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 377, P. 54 - 80
Published: Nov. 17, 2024
Language: Английский
ACS Nano, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 24, 2024
Overproduction of reactive oxygen species by damaged mitochondria after ischemia is a key factor in the subsequent cascade damage. Delivery therapeutic agents to neurons brain potentially promising targeted strategy for treatment ischemic stroke. In this study, we developed ceria nanoenzymes synergistic drug-carrying nanosystem targeting address multiple factors Each component works individually as well synergistically, resulting comprehensive therapy. Alleviation oxidative stress and modulation mitochondrial microenvironment into favorable state tolerance are combined restore bridging injuries. This work also revealed detailed mechanisms which proposed nanodelivery system protects brain, represents paradigm shift stroke treatment.
Language: Английский
Citations
43
ACS Nano, Journal Year: 2024, Volume and Issue: 18(8), P. 6702 - 6717
Published: Feb. 15, 2024
Tumor cell-derived cancer nanovaccines introduce tumor components as functional units that endow the nanovaccine systems with some advantages, especially providing all potential antigens. However, cumbersome assembly steps, risks of exogenous adjuvants, well insufficient lymph node (LN) targeting and dendritic cell (DC) internalization limit efficacy clinical translation existing nanovaccines. Herein, we introduced an endoplasmic reticulum (ER) stress inducer α-mangostin (αM) into cells through poly(d, l-lactide-co-glycolide) nanoparticles harvested biologically self-assembled (αM-Exos) based on biological process exocytosing tumor-derived exosomes (TEXs). Besides presenting multiple antigens, αM-Exos inherited abundant 70 kDa heat shock proteins (Hsp70s) upregulated by ER stress, which can not only act endogenous adjuvants but also improve LN DC internalization. Following subcutaneous injection, efficiently migrated to LNs was expeditiously endocytosed DCs, delivering antigens DCs synchronously, then powerfully triggered antitumor immune responses established long-term memory. Our study exhibited all-in-one platform, fully featured assembled this platform are promising for desirable immunotherapy.
Language: Английский
Citations
16
Cell Biology and Toxicology, Journal Year: 2025, Volume and Issue: 41(1)
Published: Feb. 24, 2025
Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles (hucMSC-sEV) have recently garnered attention as a potential therapeutic approach for kidney diseases with anti-inflammatory effects. Infiltrated macrophages play an important role in facilitating tissue regeneration. However, the intricate regulatory effects of hucMSC-sEV on during cisplatin-induced acute injury (AKI) remain unknown. In this study, we uncovered that exhibited potent anti-inflammation and effectively inhibited polarization M1 phenotype macrophages. Mechanically, miRNA sequencing analysis qRT-PCR indicated novel miRNA, named miR-13896, was enriched hucMSC-sEV. When transfected miR-13896 mimic, displayed M2 elevated levels Arg1 IL-10, while inhibitor promoted phenotype. Furthermore, firstly established repressed Tradd expression by targeting its 3' untranslated region subsequently NF-κB signaling pathway Additionally, to improve effects, were engineered through electroporation, which resulted promoting macrophages, inhibiting inflammatory factors, enhancing repair. Conclusively, our findings provide insights into mechanisms underlying AKI, also highlighting electroporation promising strategy treating AKI.
Language: Английский
Citations
3
Nano Letters, Journal Year: 2024, Volume and Issue: 24(7), P. 2131 - 2141
Published: Jan. 16, 2024
Ischemia/reperfusion (IR)-induced acute lung injury (ALI) has a high mortality rate. Reactive oxygen species (ROS) play crucial role in causing cellular damage and death IR-induced ALI. In this work, we developed biomimetic lung-targeting nanoparticle (PC@MB) as an antioxidative protector for treating PC@MBs showed excellent ROS scavenging Nrf2 activation properties, along with function through autologous cell membrane coating. The exhibited impressive pulmonary protective via redox homeostasis recovery heme oxygenase-1 activation. could maintain viability by effectively the intracellular restoring equilibrium lesion. IR mouse model, preferentially accumulated distinctly repaired pneumonic damage. Our strategy potential to offer promising therapeutic paradigm ALI incorporation of different mechanisms.
Language: Английский
Citations
12
Chinese Chemical Letters, Journal Year: 2023, Volume and Issue: 35(2), P. 109149 - 109149
Published: Sept. 27, 2023
Language: Английский
Citations
21
Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 362, P. 210 - 224
Published: Aug. 31, 2023
Language: Английский
Citations
19
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(13)
Published: Feb. 7, 2024
Abstract Effective neuroprotective agents are required to prevent neurological damage caused by reactive oxygen species (ROS) generated cerebral ischemia‐reperfusion injury (CIRI) following an acute ischemic stroke. Herein, it is aimed develop the of cerium oxide loaded with platinum clusters engineered modifications (Pt n ‐CeO 2 ). The density functional theory calculations show that Pt could effectively scavenge ROS, including hydroxyl radicals (·OH) and superoxide anions (·O − In addition, exhibits dismutase‐ catalase‐like enzyme activities, which capable scavenging hydrogen peroxide (H O in vitro studies adjust restoration mitochondrial metabolism ROS homeostasis, rebalance cytokines, feature high biocompatibility. mice CIRI demonstrate also restore cytokine levels, reduce cysteine aspartate‐specific protease (cleaved Caspase 3) induce polarization microglia M2‐type macrophages, thus inhibiting inflammatory responses. As a result, inhibits reperfusion‐induced neuronal apoptosis, relieves infarct volume, reduces severity score, improves cognitive function. Overall, these findings suggest prominent effect has significant provides potential therapeutic alternative for CIRI.
Language: Английский
Citations
8
Smart Medicine, Journal Year: 2024, Volume and Issue: 3(3)
Published: July 14, 2024
Extracellular vesicles (EVs) are lipid bilayer containing proteins, lipids, nucleic acids, and metabolites secreted by cells under various physiological pathological conditions that mediate intercellular communication. The main types of EVs include exosomes, microvesicles, apoptotic bodies (ABs). ABs released during the terminal stages cellular apoptosis, enriched with diverse biological entities characterized distinct morphological features. As a result, possess great potential in fields like disease diagnosis, immunotherapy, regenerative therapy, drug delivery due to their specificity, targeting capacity, biocompatibility. However, therapeutic efficacy is notably heterogeneous, an overdose can lead side effects such as accumulation liver, spleen, lungs, gastrointestinal system. Through bioengineering, properties be optimized enhance drug-loading efficiency, precision, multifunctionality for clinical implementations. This review focuses on strategies transfection, sonication, electroporation, surface engineering, integration biomaterials enable load cargoes targeting, providing insights into engineering ABs.
Language: Английский
Citations
7
Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)
Published: July 22, 2024
Abstract Stroke is a leading cause of mortality and long-term disability globally, with acute ischemic stroke (AIS) being the most common subtype. Despite significant advances in reperfusion therapies, their limited time window associated risks underscore necessity for novel treatment strategies. Stem cell-derived extracellular vesicles (EVs) have emerged as promising therapeutic approach due to ability modulate post-stroke microenvironment facilitate neuroprotection neurorestoration. This review synthesizes current research on potential stem EVs AIS, focusing origin, biogenesis, mechanisms action, strategies enhancing targeting capacity efficacy. Additionally, we explore innovative combination therapies discuss both challenges prospects EV-based treatments. Our findings reveal that exhibit diverse effects such promoting neuronal survival, diminishing neuroinflammation, protecting blood-brain barrier, angiogenesis neurogenesis. Various strategies, including modifications cargo modifications, been developed improve efficacy EVs. Combining other treatments, therapy, cell transplantation, nanomedicine, gut microbiome modulation, holds great promise improving outcomes. However, heterogeneity need standardized protocols EV production quality control remain be addressed. represent avenue offering address limitations Further needed optimize translate benefits clinical practice, an emphasis ensuring safety, overcoming regulatory hurdles, specificity delivery target tissues. Graphical
Language: Английский
Citations
7
International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 415 - 428
Published: Jan. 1, 2024
Purpose:The promotion of angiogenesis is an effective strategy for skin wound repair.While the transplantation endothelial cells has shown promise in vascularization, underlying mechanism remains unclear.Recent studies have suggested that transplanted undergo apoptosis a short period and release apoptotic extracellular vesicles (ApoEVs) may therapeutic potential.Methods: In this study, we isolated ApoEVs from human umbilical vein (HUVECs) characterized their properties.In vitro, assessed effects on proliferation, migration, differentiation fibroblasts.In vivo, investigated role ApoEVs-AT full-thickness wounds, evaluating closure rate, re-epithelialization, granulation tissue formation, scar area, collagen 3(Col3)/collagen 1(Col 1) ratio.Results: derived HUVECs displayed typical characteristics.In significantly enhanced tube expression angiogenic-related genes slightly promoted proliferation migration improved formation tissue, vascularization.Besides, reduced accompanied by increase Col 3/ 1 ratio.Conclusion: Given abundant source effectiveness, study provided novel approach regeneration deepened understanding death to regeneration.
Language: Английский
Citations
6