Extracellular vesicle-loaded hydrogels for tissue repair and regeneration

Materials Today Bio, Journal Year: 2022, Volume and Issue: 18, P. 100522 - 100522

Published: Dec. 21, 2022

Extracellular vesicles (EVs) are a collective term for nanoscale or microscale secreted by cells that play important biological roles. Mesenchymal stem class of with the potential self-healing and multidirectional differentiation. In recent years, numerous studies have shown EVs, especially those mesenchymal cells, can promote repair regeneration various tissues and, thus, significant in regenerative medicine. However, due to rapid clearance capacity circulatory system, EVs barely able act persistently at specific sites target tissues. Hydrogels good biocompatibility loose porous structural properties allow them serve as EV carriers, thereby prolonging retention certain areas slowing release EVs. When needed function sites, EV-loaded hydrogels stand an excellent approach. this review, we first introduce sources, roles, extraction characterization methods describe their current application status. We then review different types discuss factors influencing abilities carry summarize several strategies loading into characterizing hydrogels. Furthermore, applications tissue repair. This article concludes summary state research on outlook future directions, which hope will provide promising ideas researchers.

Language: Английский

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Recent advances in extracellular vesicles for therapeutic cargo delivery

Experimental & Molecular Medicine, Journal Year: 2024, Volume and Issue: 56(4), P. 836 - 849

Published: April 1, 2024

Abstract Exosomes, which are nanosized vesicles secreted by cells, attracting increasing interest in the field of biomedical research due to their unique properties, including biocompatibility, cargo loading capacity, and deep tissue penetration. They serve as natural signaling agents intercellular communication, inherent ability carry proteins, lipids, nucleic acids endows them with remarkable therapeutic potential. Thus, exosomes can be exploited for diverse applications, chemotherapy, gene therapy, photothermal therapy. Moreover, capacity homotypic targeting self-recognition provides opportunities personalized medicine. Despite advantages novel agents, there several challenges optimizing efficiency structural stability defining exosome origins. Future should include development large-scale, quality-controllable production methods, refinement drug strategies, extensive vivo studies clinical trials. unresolved difficulties, use efficient, stable, safe delivery systems is an interesting area research. Therefore, this review describes summarizes cutting-edge published high-impact journals that have introduced or enhanced effects using a system past 2 years. We provide informative overview current state research, highlighting properties applications exosomes. also emphasize future directions, underscoring importance addressing key issues field. With review, we encourage researchers further develop exosome-based drugs application, such may among most promising next-generation therapeutics.

Language: Английский

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Nebulised delivery of RNA formulations to the lungs: From aerosol to cytosol

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 366, P. 812 - 833

Published: Jan. 25, 2024

In the past decade RNA-based therapies such as small interfering RNA (siRNA) and messenger (mRNA) have emerged new ground-breaking therapeutic agents for treatment prevention of many conditions from viral infection to cancer. Most clinically approved are parenterally administered which impacts patient compliance adds healthcare costs. Pulmonary administration via inhalation is a non-invasive means deliver offers an attractive alternative injection. Nebulisation particularly appealing method due capacity large doses during tidal breathing. this review, we discuss unique physiological barriers presented by lung efficient nebulised delivery approaches adopted circumvent problem. Additionally, different types nebulisers evaluated perspective their suitability delivery. Furthermore, recent preclinical studies involving nebulisation analysis in vitro vivo settings. Several also demonstrated importance effective vector therefore assess variety lipid, polymeric hybrid-based systems utilised date. We consider outlook medicinal products hurdles must be overcome successful clinical translation. summary, has promising potential several lung-related asthma, COPD cystic fibrosis, mode crucial success.

Language: Английский

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Applications and advancements of nanoparticle-based drug delivery in alleviating lung cancer and chronic obstructive pulmonary disease

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2023, Volume and Issue: 397(5), P. 2793 - 2833

Published: Nov. 22, 2023

Language: Английский

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Opportunities and Challenges for Inhalable Nanomedicine Formulations in Respiratory Diseases: A Review

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 1509 - 1538

Published: Feb. 1, 2024

Abstract: Lungs experience frequent interactions with the external environment and have an abundant supply of blood; therefore, they are susceptible to invasion by pathogenic microorganisms tumor cells. However, limited pharmacokinetics conventional drugs in lungs poses a clinical challenge. The emergence different nano-formulations has been facilitated advancements nanotechnology. Inhaled nanomedicines exhibit better targeting prolonged therapeutic effects. Although great potential, still present several unknown risks. Herein, we review (1) physiological anatomy their biological barriers, (2) toxicology nanomaterial formulations lungs; (3) current nanomaterials that can be applied respiratory system related design strategies, (4) applications inhaled treating disorders, vaccine design, imaging detection based on characteristics nanomaterials. Finally, (5) analyze summarize challenges prospects for disease applications. We believe nanomaterials, particularly nano-formulations, excellent application diseases. emphasize simultaneous toxic side effects must considered during these emerging medicines. This study aims offer comprehensive guidelines valuable insights conducting research domain system. Keywords: nanoparticles, system, lungs, nanotechnology

Language: Английский

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The (re)emergence of aerosol delivery: Treatment of pulmonary diseases and its clinical challenges

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 379, P. 421 - 439

Published: Jan. 16, 2025

Aerosol delivery represents a rapid and non-invasive way to directly reach the lungs while escaping hepatic first-pass effect. The development of pulmonary drugs for respiratory diseases such as cystic fibrosis, lung infections, fibrosis or cancer requires an enhanced understanding relationships between natural physiology system pathophysiology these conditions. This knowledge is crucial better predict thereby control drug deposition. Moreover, aerosol administration faces several challenges, including tract, immune system, mucociliary clearance, presence fluid on airway surfaces, and, in some cases, bacterial colonisation. Each them influences bioavailability active molecule. In addition particle size device used administer treatment are critical factors that can significantly impact biodistribution drugs. Nanoparticles very promising new formulations delivery, they be fine-tuned entire tract overcome difficulties encountered along way. However, properly assess preclinical studies need more thorough efficiently enhance delivery.

Language: Английский

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Extracellular vesicle-encapsulated CC16 as novel nanotherapeutics for treatment of acute lung injury

Molecular Therapy, Journal Year: 2023, Volume and Issue: 31(5), P. 1346 - 1364

Published: Jan. 11, 2023

Acute lung injury (ALI) is still associated with high mortality. Growing evidence suggests that Club Cell Protein 16 (CC16) plays a protective role against ALI. However, the doses of recombinant CC16 (rCC16) used in preclinical studies are supraphysiological for clinical applications. Extracellular vesicles (EVs) nanovesicles endogenously generated by mammalian cells. Our study demonstrated released via small EVs and EV-encapsulated (sEV-CC16) has anti-inflammatory activities, which protect mice from lipopolysaccharide (LPS) or bacteria-induced Additionally, sEV-CC16 can activate DNA damage repair signaling pathways. Consistent this activity, we observed more severe lungs Cc16 knockout (KO) than wild-type (WT) mice. Mechanistically, elucidated suppresses nuclear factor κB (NF-κB) activation binding to heat shock protein 60 (HSP60). We concluded could be potential therapeutic agent ALI inhibiting inflammatory responses reducing NF-κB signaling.

Language: Английский

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The potential application of encapsulated exosomes: A new approach to increase exosomes therapeutic efficacy

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 162, P. 114615 - 114615

Published: April 1, 2023

Cell therapy is one of the methods that have shown promising results in treating diseases recent decades. However, use different types cells comes with limitations. The application immune cell can lead to cytokine storms and inappropriate responses self-antigens. Also, stem has potential create tumors. may not migrate injury site after intravenous injection. Therefore, using exosomes from as therapeutic candidates were proposed. Due their small size favorable characteristics, such biocompatibility immunocompatibility, easy storage isolation, attracted much attention. They are used many diseases, including cardiovascular orthopedic autoimmune cancer. various studies efficiency (Exo) be increased by loading drugs microRNAs inside them (encapsulated exosomes). analyzing investigating encapsulated exosomes' ability critical. In this study, we examined related cancer infectious regenerative medicine. Compared intact exosomes, show a higher ability. Therefore it suggested method depending on treatment type increase treatment's efficiency.

Language: Английский

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Accurate and Convenient Lung Cancer Diagnosis through Detection of Extracellular Vesicle Membrane Proteins via Förster Resonance Energy Transfer

Nano Letters, Journal Year: 2023, Volume and Issue: 23(17), P. 8115 - 8125

Published: Aug. 29, 2023

Tumor-derived extracellular vesicles (EVs) are promising to monitor early stage cancer. Unfortunately, isolating and analyzing EVs from a patient's liquid biopsy challenging. For this, we devised an EV membrane proteins detection system (EV-MPDS) based on Förster resonance energy transfer (FRET) signals between aptamer quantum dots AIEgen dye, which eliminated the extraction purification conveniently diagnose lung In cohort of 80 clinical samples, this showed enhanced accuracy (100% versus 65%) sensitivity 55%) in cancer diagnosis as compared ELISA method. Improved screening (from 96.4% 100%) was achieved by comprehensively profiling five biomarkers using machine learning analysis system. FRET-based tumor EV-MPDS is thus isolation-free, low-volume (1 μL), highly accurate approach, providing potential aid screening.

Language: Английский

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Delivery systems of therapeutic nucleic acids for the treatment of acute lung injury/acute respiratory distress syndrome

Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 360, P. 1 - 14

Published: June 19, 2023

Language: Английский

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