Chitosan nanoparticles as a smart nanocarrier for gefitinib for tackling lung cancer: Design of experiment and in vitro cytotoxicity study

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 246, P. 125638 - 125638

Published: June 30, 2023

Language: Английский

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Synthesis and evaluation of berberine loaded chitosan nanocarrier for enhanced in-vitro antioxidant and anti-inflammatory potential

Carbohydrate Polymer Technologies and Applications, Journal Year: 2024, Volume and Issue: 7, P. 100474 - 100474

Published: March 5, 2024

Berberine, a naturally occurring isoquinoline plant alkaloid molecule, finds application in traditional medicine due to its extensive pharmacological effects but it cannot be clinically exploited fullest as possesses poor water solubility and bioavailability. In order address this issue, we have synthesized berberine loaded chitosan nanoparticles (BCNPs) using DMSO surfactant polymer modified ionic complexation approach optimized the process utilizing central composite design. The BCNPs were empirically assessed for various parameters such particle size distribution, zeta potential, drug encapsulation efficiency, surface morphology established methodologies. additionally in-vitro antibacterial activity, antioxidant anti-inflammatory sustained release behavior techniques. Experimental results showed that at concentration of 0.05% (w/v) 1% (v/v) successfully generated with spherical shape ranging from 20 60 nm exhibited stabilizing potential 26.1 mV. Additionally, they had high effectiveness 94.01%. Sustained was observed 74.30% released 48 h, respectively BCNPs. antimicrobial assays reveal exhibit superior against all bacterial strains compared alone determined by agar well diffusion method. also substantial rise DPPH activity greater percentage protection berberine. summary, our research suggests nanocarrier can enhance facilitate development novel naturally-derived antioxidants compounds. This study will further provide an insight into future natural discovery.

Language: Английский

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Enhanced oral delivery of hesperidin-loaded sulfobutylether-β-cyclodextrin/chitosan nanoparticles for augmenting its hypoglycemic activity: in vitro-in vivo assessment study

Drug Delivery and Translational Research, Journal Year: 2023, Volume and Issue: 14(4), P. 895 - 917

Published: Oct. 16, 2023

Abstract Hesperidin (Hsd), a bioactive phytomedicine, experienced an antidiabetic activity versus both Type 1 and 2 Diabetes mellitus . However, its intrinsic poor solubility bioavailability is key challenging obstacle reflecting oral delivery. From such perspective, the purpose of current study was to prepare evaluate Hsd-loaded sulfobutylether-β-cyclodextrin/chitosan nanoparticles (Hsd/CD/CS NPs) for improving hypoglycemic orally administered Hsd. Hsd first complexed with sulfobutylether-β-cyclodextrin (SBE-β-CD) complex (CX) found be formed percent complexation efficiency process 50.53 ± 1.46 84.52 3.16%, respectively. Also, solid state characterization ensured inclusion inside cavity SBE-β-CD. Then, Hsd/CD/CS NPs were prepared using ionic gelation technique. The fully characterized select most promising one (F1) homogenous particle size 455.7 9.04 nm, positive zeta potential + 32.28 1.12 mV, entrapment 77.46 0.39%. optimal formula subjected further investigation in vitro release, ex vivo intestinal permeation, stability, cytotoxicity, activity. results release permeation studies F1 manifested modulated pattern between CX. preferential stability observed at 4 °C. biocompatibility epithelial cell line (OEC) retained up concentration 100 µg/mL. After administration F1, noteworthy synergistic effect recorded decreased blood glucose level until end experiment. In conclusion, could regarded as hopeful delivery system enhanced Graphical

Language: Английский

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99mTc-radiolabeled sunitinib encapsulated chitosan nanoparticles: an innovative radiopharmaceutical-based approach in cancer treatment

Radiation effects and defects in solids, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 17

Published: April 4, 2025

Language: Английский

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Enhancing the Bioavailability of the Ellagitannin, Geraniin: Formulation, Characterization, and in vivo Evaluation

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: 660, P. 124333 - 124333

Published: June 12, 2024

Geraniin (GE), an ellagitannin (ET) renowned for its promising health advantages, faces challenges in practical applications due to limited bioavailability. This innovative and novel formulation of GE soy-phosphatidylcholine (GE-PL) complex has the potential increase oral bioavailability, exhibiting high entrapment efficiency 100.2 ± 0.8 %, complexation 94.6 1.1 %. The small particle size (1.04 0.11 μm), low polydispersity index (0.26 0.02), adequate zeta (−26.1 0.12 mV), indicate uniformity stability. Moreover, also demonstrates improved lipophilicity, reduced aqueous buffer solubilities, better partition coefficient. It been validated by various analytical techniques, including Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), X-ray diffraction (XRD) studies. Oral bioavailability pharmacokinetics free GE-PL investigated rabbits demonstrated enhanced plasma concentration ellagic acid (EA) compared GE. Significantly, GE, whether form or as part complex, was not found circulatory system. However, EA levels were observed at 0.5 h after administration, displaying two distinct peaks 2 0.03 (T1max) 24 0.06 (T2max). These corresponded peak concentrations (C1max C2max) 588.82 ng/mL 711.13 respectively, signifying substantial 11-fold 5-fold enhancements when Additionally, it showed increased area under curve (AUC), elimination half-life (t1/2, el) rate constant (Kel). prolonged presence bloodstream absorption, ultimately leading a higher In summary, study highlights significance overcoming limitations paving way therapeutic outcomes drug delivery healthcare.

Language: Английский

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Natural product-based excipients for topical green formulations

Sustainable Chemistry and Pharmacy, Journal Year: 2023, Volume and Issue: 33, P. 101111 - 101111

Published: June 1, 2023

Language: Английский

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Sodium alginate polymer nanoformulation as promising carrier for berberine delivery: Synthesis, morphology and in-vitro evaluation

Carbohydrate Polymer Technologies and Applications, Journal Year: 2023, Volume and Issue: 7, P. 100408 - 100408

Published: Dec. 14, 2023

The present study focused on the fabrication of berberine sodium alginate nanoparticles with a combination approach conventional nanoprecipitation method ionic complexation to improve its poor water solubility. optimization nanoformulation was done by Central Composite Design and evaluated for DLS, entrapment efficiency, morphology, FTIR, DSC, antioxidant, anti-inflammatory, antibacterial efficacy, drug release kinetics. particle size optimized in range 20 nm 100 an efficiency 94.47 %. Antibacterial efficacy checked measuring zone inhibition both gram-positive bacteria gram-negative bacteria. showed enhanced antimicrobial activity compared control against all bacterial strains. Also, sustained over about 48 hours achieved nanoformulation; followed zero-order Thus, can potentially increase oral bioavailability be utilized controlled delivery thereby reducing side effects.

Language: Английский

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Characterization and antioxidant activity of nano-formulated berberine and cyperus rotundus extracts with anti-inflammatory effects in mastitis-induced rats

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 9, 2024

Bovine mastitis caused by infectious pathogens, mainly Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), constitutes a major destructive challenge for the dairy industry public health. Berberine chloride (BER) Cyperus rotundus possess broad spectrum of anti-inflammatory, antioxidant, antibacterial, antiproliferative activities; however, their bioavailability is low. This research aimed first to prepare an ethanolic extract rhizomes (CRE) followed screening its phytochemical contents, then synthesis BER CRE loaded chitosan nanoparticles (NPs) (BER/CH-NPs CRE/CH-NPs), afterward, analysis loading efficiency in addition morphological physicochemical characterization formulated NPs employing Scanning Electron Microscopy (SEM), Zeta Potential (ZP), Fourier Transform Infrared Spectroscopy (FTIR), Differential Calorimetry (DSC) X-Ray Diffraction (XRD) assessments compared crude forms evaluate enhancement stability. Isolation bacterial strains from milk mastitic cows, used induction mammary gland (MG) inflammation female albino rats, preliminary investigation prophylactic oral doses prepared against S. aureus-induced rats. The minimal inhibitory concentration (MIC) BER/CH-NPs CRE/CH-NPs 1 mg/kg b.w. alone or combination show significant (P ≤ 0.05) DPPH radical scavenging activity (69.2, 88.5, 98.2%, respectively) vitro. Oral administration rats significantly attenuated TNF-α (22.1, 28.6 pg/ml), IL-6 (33.4, 42.9 IL-18 (21.7, 34.7 IL-4 (432.9, 421.6 MPO (87.1, 89.3 pg/ml) group alongside improvement MG histopathological findings without any side effect on renal hepatic functions. Despite promising results with nanoparticles, study limited small-scale trials, focus acute administration, partially explored nanoparticle-biological interactions, no economic scalability assessments. Future should address these limitations expanding trial scopes, exploring interactions further, extending durations, assessing practical scalability. Field trials regulatory compliance are also necessary ensure application safety industry. In conclusion, vitro vivo proved antioxidant anti-inflammatory efficacy low damage liver kidney functions, supposing uses treatment.

Language: Английский

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Edoxaban enfolded beta-1,4-Poly-D-glucosamine nanoparticles for targeting ponym Stuart–Prower factor for treatment of venous thrombosis

Journal of drug targeting, Journal Year: 2024, Volume and Issue: 32(9), P. 1125 - 1138

Published: July 11, 2024

The present research looked for ways to develop shielded nanoparticles (NPs)-drug transporters made of chitosan (CS) enhance the bioavailability edoxaban tosylate monohydrate (ETM) oral administration by examining correlation among design aspects and data from experiments using response surface methodology (RSM). ETM-loaded CS (ETM-CS-NPs) were developed ionic gelation with tripolyphosphate (TPP). Utilising Zeta-sizer scanning electron microscopy, ETM-CS-NPs evaluated particle size (PS), zeta potential (ZP), morphology, polydispersity index (PDI), entrapment efficiency (EE) drug loading (DL). Drug polymer interactions in NPs assessed Fourier transform infra-red spectroscopy. approach Design-Expert software optimised ETM-CS-NPs. Using RSM, effects independent variables such as amount CS, TPP, glacial acetic acid on PS, PDI ZP analysed. optimal combination PS (354.8 nm), (0.509), (43.7 + mV), % EE (70.3 ± 1.3) DL (9.1 0.4) has been identified ETM-CS-NPs' anticoagulant activity was activated partial thromboplastin time (aPTT), prothrombin (PT) thrombin (TT) assays. In conclusion, a practical consistent method established, its application proven

Language: Английский

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Surface-modified liposomal in-situ nasal gel enhances brain targeting of berberine hydrochloride for Alzheimer’s therapy: optimization and in vivo studies

Journal of Liposome Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 18

Published: Nov. 25, 2024

This work aimed to formulate surface-modified berberine hydrochloride (BER)-loaded liposomes containing

Language: Английский

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