Gallic Acid-Encapsulated PAMAM Dendrimers as an Antioxidant Delivery System for Controlled Release and Reduced Cytotoxicity against ARPE-19 Cells DOI Creative Commons

Aorada Sripunya,

Chuda Chittasupho,

Supachoke Mangmool

et al.

Bioconjugate Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Poly(amidoamine) (PAMAM) dendrimers have gained significant attention in various research fields, particularly medicinal compound delivery. Their versatility lies their ability to conjugate with functional molecules on surfaces and encapsulate small molecules, making them suitable for diverse applications. Gallic acid is a potent antioxidant that has garnered considerable interest recent years. Our aims investigate if the gallic acid-encapsulated PAMAM dendrimer generations 4 (G4(OH)-Ga) 5 (G5(OH)-Ga) could enhance radical scavenging, which potentially slow down progression of age-related macular degeneration (AMD). Encapsulation feasible alternative prevent its degradation toxicity.

Language: Английский

Lipidic and Inorganic Nanoparticles for Targeted Glioblastoma Multiforme Therapy: Advances and Strategies DOI Creative Commons
Ewelina Musielak, Violetta Krajka‐Kuźniak

Micro, Journal Year: 2025, Volume and Issue: 5(1), P. 2 - 2

Published: Jan. 3, 2025

Due to their biocompatibility, nontoxicity, and surface conjugation properties, nanomaterials are effective nanocarriers capable of encapsulating chemotherapeutic drugs facilitating targeted delivery across the blood–brain barrier (BBB). Although research on nanoparticles for brain cancer treatment is still in its early stages, these systems hold great potential revolutionize drug delivery. Glioblastoma multiforme (GBM) one most common lethal tumors, heterogeneous aggressive nature complicates current treatments, which primarily rely surgery. One significant obstacles poor penetration BBB. Moreover, GBM often referred as a “cold” tumor, characterized by an immunosuppressive tumor microenvironment (TME) minimal immune cell infiltration, limits effectiveness immunotherapies. Therefore, developing novel, more treatments critical improving survival rate patients. Current strategies enhancing outcomes focus controlled, agents cells BBB using nanoparticles. These therapies must be designed engage specialized transport systems, allowing efficient penetration, improved therapeutic efficacy, reduced systemic toxicity degradation. Lipid inorganic can enhance while minimizing side effects. formulations may include epitopes—small antigen fragments that bind directly free antibodies, B receptors, or T receptors—that interact with enable crossing, thereby boosting efficacy. Lipid-based (LNPs), such liposomes, niosomes, solid lipid (SLNs), nanostructured carriers (NLCs), among promising due unique including size, modification capabilities, proven biosafety. Additionally, gold nanoparticles, mesoporous silica, superparamagnetic iron oxide dendrimers offer alternatives. Inorganic (INPs) easily engineered, surfaces modified various elements biological ligands delivery, biocompatibility. Strategies engineering functionalization have been employed ensure biocompatibility reduce cytotoxicity, making safer clinical applications. The use INPs has shown promise efficacy traditional like chemotherapy, radiotherapy, gene therapy, well advancing newer strategies, immunotherapy, photothermal photodynamic therapies, magnetic hyperthermia. This article reviews latest treating GBM, focusing active passive targeting approaches.

Language: Английский

Citations

3
Nanotechnology-Based Herbal Medicine: Preparation, Synthesis, and Applications in Food and Medicine DOI Creative Commons
Farhang Hameed Awlqadr, Kithar Rasheed Majeed, Ammar B. Altemimi

et al.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101661 - 101661

Published: Jan. 1, 2025

Language: Английский

Citations

3
Deuterated Nanopolymers for Renal and Lymphatic Imaging via Quantitative Deuterium MRI DOI Creative Commons
Lisa M. Fries, Elton Tadeu Montrazi,

Hyla Allouche‐Arnon

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Deuterium (2H) MRI is an emerging tool for noninvasive imaging. We explore the integration of 2H with deuterated multifunctional nanopolymers particle imaging (DPI). To this end, amine-terminated G5-polyamidoamine (PAMAM) dendrimers were labeled acetyl surface groups, leading to highly 2H-loaded bioparticles, making them ideal studies. The accumulation ∼5 nm PAMAM in kidneys could then be seen by high submillimeter resolution. natural abundance HDO signal provided internal concentration reference these measurements, quantitative dynamic maps showing distinct nanopolymer uptakes within renal compartments. Further, allowed us obtain vivo activity lymph nodes inflammatory rodent leg model, demonstrating nanopolymers' potential as a novel class contrast agents mapping physiological processes.

Language: Английский

Citations

1
Advancements in Nanoparticle-Based Strategies for Enhanced Antibacterial Interventions DOI
Madineh Moradialvand, Nastaran Asri,

Mahtab Jahdkaran

et al.

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 82(4), P. 3071 - 3090

Published: July 18, 2024

Language: Английский

Citations

7
Spectroscopic insights into human serum albumin and PGSA dendrimer interactions: assessing bio-distribution efficiency DOI
M. Suresh Kumar, S. Karthikeyan, Fathi S. Awad

et al.

Chemical Papers, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Language: Английский

Citations

0
Nanobody-Mediated Cellular Uptake Maximizes the Potency of Polylysine Dendrimers While Preserving Solid Tumor Penetration DOI
Daniel Yuen, Orlagh M. Feeney,

Leo Noi

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Dendrimers are branched macromolecular structures that useful nanocarriers for small-molecule drugs, such as cancer therapeutics. Their small size permits penetration into solid tumors, coupled with functionalization a low-fouling PEG coating minimizes transient cellular interactions and enhances plasma circulation time. While PEGylated dendrimers show significant promise anticancer therapeutics, there is potential to increase tumor cell specificity drive uptake of drugs cells by conjugating cell-targeting ligands onto the dendrimers. To achieve this, we used an expanded genetic code bio-orthogonal click chemistry functionalize monomethyl auristatin E (MMAE)-loaded single nanobody per dendrimer. The uniform addition ligand facilitated greater intracellular drug payload HER2-positive target cells, while preserving desirable circulatory characteristics nanobody–dendrimer conjugates similar levels infiltration over 24 h compared unmodified dendrimers, targeted had significantly inhibition growth long-term retention in tumors. Our results highlight biodistribution studies alone poor predictors therapeutic performance. controlled conjugation strategy presented here preserves advantage tissue maximizing potency difficult-to-access tissue.

Language: Английский

Citations

0
Human Embryonic Kidney HEK293 Cells as a Model to Study SMVT-Independent Transport of Biotin and Biotin-Furnished Nanoparticles in Targeted Therapy DOI Open Access
Magdalena Twardowska, Andrzej Łyskowski, Maria Misiorek

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1594 - 1594

Published: Feb. 13, 2025

The aim of this study was to investigate the usefulness human embryonic kidney HEK293 cells as a model normal in biotin-mediated therapy. expression and role sodium multivitamin transporter (SMVT) uptake accumulation free biotin, well cationic neutral biotinylated PAMAM dendrimers fourth generation synthesized our laboratory, were assessed comparison other immortalized (HaCaT) cancer (HepG2, U-118 MG). obtained data showed that higher level SMVT not associated with stronger biotin dendrimers. Biotinylation increased selective an inversely proportional manner concentration used; however, lower than cell lines. time-dependent profiles differed significantly. Therefore, it should be assumed efficiency nanoparticles' depends on multiple cellular transport mechanisms. Toxicity tests significantly sensitivity conjugates for HepG2 HaCaT cells. Molecular modeling studies profile suggest only but also monocarboxylate 1 (MCT-1) may play important nanoparticles into Due complexity problem, further are necessary. In summary, can considered valuable biotin- targeted therapy using based

Language: Английский

Citations

0
Evaluating the use of lanthanide containing dendrimers for solvent paramagnetic relaxation enhancement DOI Creative Commons
Westley Pawloski, James M. Gruschus, Ana Christina L. Opina

et al.

Journal of Biomolecular NMR, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Abstract Paramagnetic relaxation enhancement (PRE) is widely used in biomolecular NMR spectroscopy to obtain long-range distance and orientational information for intra- or intermolecular interactions. In contrast conventional PRE measurements, which require tethering small molecules containing either a radical paramagnetic ion specific sites on the target protein, solvent (sPRE) experiments utilize cosolutes induce delocalized effect. Compounds developed as agents magnetic resonance imaging (MRI) applications typically consist of Gd chelated by molecule. Coordinating these Gd-containing larger inert scaffolds has been shown increase PRE-effect produce more effective MRI. Inspired their use MRI agent, this work we evaluate effectiveness using functionalized polyamidoamine (PAMAM) dendrimer sPRE measurements. Using ubiquitin model system, measured effect from generation 5 PAMAM (G5-Gd) function temperature pH compared agents. We also demonstrated utility G5-Gd studies monitor changes structures two proteins they bind ligands. These highlight attractive properties macromolecular sPRE.

Language: Английский

Citations

0
Recent advances in graphitic carbon nitride-based heterojunction for biomedical applications DOI
Ruilong Jia, Chengen He,

Shan-Shan Wang

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 500, P. 157464 - 157464

Published: Nov. 1, 2024

Language: Английский

Citations

3
Advances in Dendritic Systems and Dendronized Nanoparticles: Paradigm Shifts in Cancer Targeted Therapy and Diagnostics DOI

Pawan Kedar,

Apeksha Saraf,

Rahul Maheshwari

et al.

Molecular Pharmaceutics, Journal Year: 2024, Volume and Issue: 22(1), P. 28 - 57

Published: Dec. 21, 2024

Cancer has emerged as a global health crisis, claiming millions of lives annually. Dendrimers and dendronized nanoparticles, novel class nanoscale molecules with highly branched three-dimensional macromolecular structures, have gained significant attention in cancer treatment diagnosis due to their unique properties. These dendritic macromolecules offer precisely controlled branching architecture, enabling functionalization specific targeting enhance the selective delivery therapeutic agents tumor cells while minimizing systemic toxicity. Through surface modifications incorporation various components, dendrimers demonstrate remarkable adaptability nanocarriers for biomedical imaging theranostic applications. Surface strategies, including PEGylation ligand attachment (e.g., folic acid, RGD peptide, lactobionic acid), further biocompatibility facilitate targeted cell imaging. Leveraging improved target specificity, nanosystems heightened sensitivity precision diagnostics. Notably, encapsulation metal nanoparticles within dendrimers, such gold shown promise enhancing capabilities. Ongoing advancements nanotechnology are poised increase sophistication complexity dendrimer-based systems, highlighting potential drug platforms, growing number clinical trials on horizon. This review provides comprehensive overview future prospects therapy diagnosis, exploring ability biocompatibility, reduce toxicity, improve outcomes across malignancies.

Language: Английский

Citations

3