Lawsonia inermis (Henna) Extracts as a Sustainable Solution to Combat Multidrug-Resistant Gram-Negative Bacteria in Surface Water, Sewage, and Abattoir Leachate: A Quantitative Antimicrobial Analysis DOI Creative Commons
Morufat Oluwatosin Olaitan, Blessing Ifeoma Nwadike

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Abstract Antimicrobial resistance (AMR) poses a critical global public health threat, exacerbated by the presence of multidrug-resistant (MDR) bacteria in environmental matrices, particularly Low- and Middle-Income Countries (LMICs). This study investigated potential Lawsonia inermis (Henna) extracts to inhibit MDR surface water, sewage, abattoir leachate. Using cold maceration extraction method, methanol aqueous as solvent, antibacterial activity Henna leaf seed was assessed against eight isolates obtained from The results showed exceptional antimicrobial activity, especially with demonstrating 100% inhibition bacteria, followed (93.7%). minimum inhibitory concentration (MIC) bactericidal (MBC) values confirmed efficacy extracts. All displayed profound (AI) S. enterica AGS2 strain. Citrobacter freundii ADAI most susceptible isolate. Our findings suggest that could serve viable, eco-friendly, cost-effective solution for reducing LMICs. highlights plant-based products combating AMR matrices promoting health. have significant implications development sustainable leachate water treatment strategies control LMICs through solutions.

Language: Английский

The Impact of Wastewater on Antimicrobial Resistance: A Scoping Review of Transmission Pathways and Contributing Factors DOI Creative Commons

Maria Clara La Rosa,

Andrea Maugeri, Giuliana Favara

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(2), P. 131 - 131

Published: Jan. 26, 2025

Background/Objectives: Antimicrobial resistance (AMR) is a global issue driven by the overuse of antibiotics in healthcare, agriculture, and veterinary settings. Wastewater treatment plants (WWTPs) act as reservoirs for antibiotic-resistant bacteria (ARB) antibiotic genes (ARGs). The One Health approach emphasizes interconnectedness human, animal, environmental health addressing AMR. This scoping review analyzes wastewater’s role AMR spread, identifies influencing factors, highlights research gaps to guide interventions. Methods: followed PRISMA-ScR guidelines. A comprehensive literature search was conducted across PubMed Web Science databases articles published up June 2024, supplemented manual reference checks. focused on wastewater source AMR, including hospital effluents, industrial urban sewage, agricultural runoff. Screening selection were independently performed two reviewers, with conflicts resolved third. Results: Of 3367 studies identified, 70 met inclusion criteria. findings indicated that residues, heavy metals, microbial interactions are key drivers development. Although WWTPs aim reduce contaminants, they often create conditions conducive horizontal gene transfer, amplifying resistance. Promising interventions, such advanced methods regulatory measures, exist but require further implementation. Conclusions: plays pivotal dissemination. Targeted interventions management essential mitigate risks. Future should prioritize understanding dynamics ecosystems evaluating scalable mitigation strategies support efforts.

Language: Английский

Citations

2
Optimization of the production of Exopolysaccharide (EPS) from biofilm-forming bacterial consortium using different parameters DOI Creative Commons
Km Jyoti, Kuldeep Soni, Ram Chandra

et al.

The Microbe, Journal Year: 2024, Volume and Issue: 4, P. 100117 - 100117

Published: July 4, 2024

Bacterial cells produce a variety of exopolysaccharide (EPS) throughout their life cycle. EPS plays crucial role in the formation biofilms and control environmental processes microbial communities. The bacterial community benefits from several ways, including nutrient compartmentation storage, stress resistance synergism. generation by consortium-associated bacteria, which involves microorganisms, is versatile efficient for use industrial processes, such as biotechnology pollution degradation. qualitative quantitative chemical characteristics consortium's are mostly determined type culture utilized, type, temperature, pH. main goal study was to find out how biosynthesis works consortium culture. To achieve maximum regeneration, pH, carbon, nitrogen sources were optimized. A three strains i.e. Klebsiella pneumonia strain, Pseudomonas aeruginosa strain Burkholderia cepacian that distinguished capacity developed part study. total production detected after 8 days incubation at pH 7.5 (15.5 g/L) temperature 35 °C (12.60 g/L). optimum identified 17.84 g/L 21.07 when ammonium sulfate glucose employed carbon supplements, respectively. protein concentration 3067 µg/mL, more than carbohydrate (263.10 µg/mL) DNA (7.60 content. protein/carbohydrate ratio present 11.65, much higher prior investigations. FTIR analysis confirmed functional groups carbohydrates, proteins, lipids DNA, viz., CO, COOH, NH, OH, CH OCH3. study's findings revealed enhancing molecules lowering biofilm-related infections improving efficacy antimicrobial treatments wastewater.

Language: Английский

Citations

14
Occurrence and dissemination of antibiotics and antibiotic resistance in aquatic environment and its ecological implications: a review DOI
Anjali Singh, Shalini G Pratap, Abhay Raj

et al.

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(35), P. 47505 - 47529

Published: July 19, 2024

Language: Английский

Citations

14
Metagenomic analysis reveals the effects of potassium ferrate and steel slag on fate of ARGs in anaerobic sludge digestion system DOI

Yali Wang,

Qiushuo Zhang,

Hang Li

et al.

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 373, P. 123981 - 123981

Published: Jan. 1, 2025

Language: Английский

Citations

1
Pharmaceutical industrial wastewater exhibiting the co-occurrence of biofilm-forming genes in the multidrug-resistant bacterial community poses a novel environmental threat DOI
Km Jyoti, Kuldeep Soni, Ram Chandra

et al.

Aquatic Toxicology, Journal Year: 2024, Volume and Issue: 273, P. 107019 - 107019

Published: July 2, 2024

Language: Английский

Citations

7
Assessment of bacteriological and immunological markers in urinary tract infection and the effect of antibiotics on the isolated bacteria DOI Creative Commons

Wisam F. Hameed,

Bashar Sadeq Noomi,

Abd Al. Kareem Khaleel

et al.

Cellular and Molecular Biology, Journal Year: 2025, Volume and Issue: 70(12), P. 125 - 134

Published: Jan. 12, 2025

Language: Английский

Citations

0
Pollution by Antimicrobials and Antibiotic Resistance Genes in East Africa: Occurrence, Sources, and Potential Environmental Implications DOI Creative Commons

Miraji Hossein,

Asha Ripanda

Toxicology Reports, Journal Year: 2025, Volume and Issue: 14, P. 101969 - 101969

Published: Feb. 22, 2025

Language: Английский

Citations

0
Phytotoxicity and genotoxicity assessment of organic and inorganic contaminants detected in pharmaceutical industrial wastewaters using Vigna radiata and Allium cepa DOI Open Access
Km Jyoti, Kuldeep Soni, Ram Chandra

et al.

Journal of Experimental Biology and Agricultural Sciences, Journal Year: 2024, Volume and Issue: 12(1), P. 76 - 92

Published: March 15, 2024

The discharged effluent of pharmaceutical industrial wastewater treatment plants (PIWWTPs) exhibits substantial environmental toxicity due to the intricate combination organic and inorganic pollutants. This study assessed phytotoxicity, genotoxicity, cytotoxicity untreated treated (PIWW). Most physicochemical parameters viz. COD, BOD, EC, sulfide, sulfate, nitrate, phosphate, grease, phenols, metal concentrations B, Cr, Ca, Cd, Cu, Zn, Pb, Hg, As in (UTW) were noted beyond permissible limit remained higher (TW). findings revealed that performance PIWWTP was woefully inadequate. GC-MS spectra UTW TW presence various contaminants. toxicological studies showed had a high degree which persisted even after as it inhibited seed germination Vigna radiata. up 70% 50% tested at concentration respectively. Genotoxicity measured by determining mitotic index chromosomal aberrations Allium cepa root apex grown PIWW. Compared negative control, dropped 85% 75% TW, Chromosomal also found cellular mass growing both TW. According findings, is unsafe for environment release PIWW has not been properly treated, this could pose serious risks health.

Language: Английский

Citations

2
Phylogenetic group distribution and antibiotic resistance of <i>Escherichia coli</i> isolates in aquatic environments of a highly populated area DOI Creative Commons

Rosette Mansour,

Mohammad H. El‐Dakdouki, Sara Mina

et al.

AIMS Microbiology, Journal Year: 2024, Volume and Issue: 10(2), P. 340 - 362

Published: Jan. 1, 2024

<abstract><sec> <title>Background</title> <p>Extended-spectrum beta-lactamase (ESBL)-producing <italic>Enterobacteriaceae</italic> including <italic>Escherichia coli</italic> (<italic>E. coli</italic>), are recognized as a global public health threat due to their multidrug-resistant (MDR) phenotypes and rapid dissemination in aquatic environments. Nevertheless, studies investigating the prevalence antimicrobial resistance (AMR) profile of ESBL-producing <italic>E. Lebanese surface water limited.</p> </sec><sec> <title>Objective</title> <p>This study aimed assess physicochemical properties microbial contamination load determine distribution AMR patterns samples from different sites North Governorate Lebanon.</p> <title>Methods</title> <p>Water were collected 25 major Lebanon. These analyzed for presence total coliforms, coli</italic>, fecal enterococci. Phenotypic genetic characterizations then performed isolates phylogenetic groups.</p> <title>Results</title> <p>Fifty-six out 100 positive mostly harboring bla<sub>CTX-M</sub> (40/56, 71%) bla<sub>CTX-M-15</sub> (33/40, 82%), bla<sub>TEM</sub> gene (36/56, 64%), bla<sub>SHV</sub> (20/56, 36%), bla<sub>OXA</sub> (16/56, 29%) bla<sub>OXA-48</sub> (11/16, 69%). Most belonged extra-intestinal pathogenic phylogroup B2 71.4%) while 10/56 (17.9%) commensal A.</p> <title>Conclusion</title> <p>Our results highlight need implement effective monitoring strategies control transmission thus reduce burden on human animal health.</p> </sec></abstract>

Language: Английский

Citations

2
Coexistence of multidrug resistance and ESBL encoding genes - blaTEM, blaSHV, and blaCTX-M; its amplification and dispersion in the environment via municipal wastewater treatment plant DOI
Kuldeep Soni, Km Jyoti,

Anil Kumar

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 362, P. 142829 - 142829

Published: July 9, 2024

Language: Английский

Citations

1