Salidroside, a phenyl ethanol glycoside from Rhodiola crenulata, orchestrates hypoxic mitochondrial dynamics homeostasis by stimulating Sirt1/p53/Drp1 signaling

Journal of Ethnopharmacology, Journal Year: 2022, Volume and Issue: 293, P. 115278 - 115278

Published: April 16, 2022

Language: Английский

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A New Therapeutic Trend: Natural Medicine for Ameliorating Ischemic Stroke via PI3K/Akt Signaling Pathway

Molecules, Journal Year: 2022, Volume and Issue: 27(22), P. 7963 - 7963

Published: Nov. 17, 2022

Ischemic stroke (IS) is an acute cerebrovascular disease caused by sudden arterial occlusion, which characterized a high morbidity, mortality, and disability rate. It one of the most important causes nervous system morbidity mortality in world. In recent years, search for new medicine treatment IS has become attractive research focus. Due to extremely limited time window traditional treatment, some side effects may occur, accompanied occurrence adverse reactions, frequency exploration with natural significantly increased. Phosphatidylinositol-3-kinase/Protein kinase B (PI3K/Akt) signaling pathway classical cell metabolism, growth, apoptosis, other physiological activities. There considerable on that treats various diseases through this pathway. This review focuses how medicines (including herbs insects) regulate pathophysiological processes such as inflammation, oxidative stress, autophagy PI3K/Akt pathway, role it plays improving IS. We found many kinds herbal insect can alleviate damage Moreover, prescription after their combination also achieve certain results. Therefore, provides candidate category development

Language: Английский

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P‑hydroxybenzyl alcohol ameliorates neuronal cerebral ischemia‑reperfusion injury by activating mitochondrial autophagy through SIRT1

Molecular Medicine Reports, Journal Year: 2023, Volume and Issue: 27(3)

Published: Feb. 8, 2023

Mitochondrial autophagy serves a key role in clearing damaged mitochondria. P‑hydroxybenzyl alcohol (pHBA) can improve neuronal injury induced by cerebral ischemia‑reperfusion (I/R). However, the mechanism of pHBA improving I/R damage through mitochondrial pathway remains unclear. A rat model middle artery occlusion and reperfusion (MCAO/R) was used present study. The rats were treated with sirtuin 1 (SIRT1) inhibitor EX527 for 7 days, followed reperfusion. At 24 h after reperfusion, infarct size calculated severity nerve evaluated. Hematoxylin eosin Nissl staining revealed cellular changes ischemic penumbra. Changes structure observed using electron microscopy. function evaluated detecting membrane potential (MMP), permeability transition pore (mPTP) ATP levels commercially available kits. In addition, penumbra tissues immunofluorescence p62 LC3 proteins. expression SIRT1 autophagy‑related proteins, PTEN‑induced kinase (PINK1) Parkin, detected western blotting. Finally, apoptosis analyzed TUNEL apoptosis‑related proteins (Bax, Bcl‑2 Caspase‑3) results suggested that postoperative treatment may reduce infarction to nervous system, cell MCAO/R rats. Compared untreated group, pHBA‑treated group improved, MMP increased, degree opening mPTP decreased. Simultaneously, blotting showed compared number LC3‑ TUNEL‑positive cells p62‑positive decreased, protein (PINK1, Parkin II/I) increased decreased group. these improvements blocked EX527. summary, from study SIRT1‑activated mitochondrial‑mediated apoptosis.

Language: Английский

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β-asarone improves cognitive impairment and alleviates autophagy in mice with vascular dementia via the cAMP/PKA/CREB pathway

Phytomedicine, Journal Year: 2023, Volume and Issue: 123, P. 155215 - 155215

Published: Nov. 26, 2023

Language: Английский

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Neuroprotective effects of exosomes derived from bone marrow mesenchymal stem cells treated by Musk Ketone on ischemic stroke

Journal of Stroke and Cerebrovascular Diseases, Journal Year: 2024, Volume and Issue: 33(8), P. 107628 - 107628

Published: Feb. 9, 2024

Language: Английский

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Electroacupuncture alleviates damage to myopic RGCs probably through lncRNA-XR_002789763.1-mediated mitophagy

Chinese Medicine, Journal Year: 2025, Volume and Issue: 20(1)

Published: Feb. 2, 2025

Abstract Background Mitophagy is closely related to the regulation of retinal ganglion cell (RGC) structure and function. Our previous study suggested that long noncoding RNAs (lncRNAs) can cause damage myopic RGCs. However, whether electroacupuncture (EA) treatment delay myopia progression through lncRNA-mediated mitophagy in RGCs currently unknown. This aimed investigate effect EA on Methods investigated modulatory guinea pigs with form-deprived (FDM). RNA sequencing was performed further analyze expression profiles lncRNAs mRNAs FDM after treatment, competing endogenous (ceRNA) network constructed. Importantly, PINK1, a mitophagy-related gene, included core ceRNA explore relationship between regulated by EA. We also collected eyeballs from highly adults verify mechanistic results. Results demonstrated delayed reduction refraction increase axial length alleviated RGC FDM. found could induce pig promoting PINK1/Parkin signaling pathway. Moreover, inhibited retina adults. revealed 599 455 were differentially expressed treatment. A constructed incorporating PINK1 verified molecular experiments, we may attenuated injury sponging miR-342-5p lncRNA-XR_002789763.1 activate pathway promote Mfn2 ubiquitination. Conclusion might regulate lncRNA-XR_002789763.1/miR-342-5p axis pathway, ubiquitination, thereby alleviating delaying progression.

Language: Английский

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Genetic deletion of G protein-coupled receptor 56 aggravates traumatic brain injury through the microglial CCL3/4/5 upregulation targeted to CCR5

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 15, 2025

Abstract Traumatic brain injury (TBI) is a significant global health concern that often results in death or disability, and effective pharmacological treatments are lacking. G protein-coupled receptor 56 (GPR56), potential drug target, crucial for neuronal glial cell function therefore plays important roles various neurological diseases. Here, we investigated the role mechanism of GPR56 TBI-related damage to gain new insights into treatment TBI. Our study revealed TBI caused decrease expression deletion Gpr56 exacerbated deficits blood‒brain barrier (BBB) following Additionally, led increased microgliosis, infiltration peripheral T cells macrophages, release cerebral inflammatory cytokines chemokines after Furthermore, induced apoptosis, impaired autophagy, through microglial-to-neuronal CCR5 signaling Overall, these indicate knockout exacerbates deficits, neuroinflammation apoptosis microglial CCL3/4/5 upregulation targeted CCR5, which indicates GRP56 may be target

Language: Английский

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Progress of medicinal plants and their active metabolites in ischemia-reperfusion injury of stroke: a novel therapeutic strategy based on regulation of crosstalk between mitophagy and ferroptosis

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: April 8, 2024

The death of cells can occur through various pathways, including apoptosis, necroptosis, mitophagy, pyroptosis, endoplasmic reticulum stress, oxidative ferroptosis, cuproptosis, and disulfide-driven necrosis. Increasing evidence suggests that mitophagy ferroptosis play crucial regulatory roles in the development stroke. In recent years, incidence stroke has been gradually increasing, posing a significant threat to human health. Hemorrhagic accounts for only 15% all strokes, while ischemic is predominant type, representing 85% cases. Ischemic refers clinical syndrome characterized by local ischemic-hypoxic necrosis brain tissue due cerebrovascular disorders, leading rapid onset corresponding neurological deficits. Currently, specific therapeutic approaches targeting pathophysiological mechanisms injury mainly include intravenous thrombolysis endovascular intervention. Despite some efficacy, these inevitably lead ischemia-reperfusion injury. Therefore, exploration treatment options remains challenging task. light this background, advancements targeted therapy diseases offer new direction such diseases. review, we summarize progress regulating emphasize their potential molecular pathogenesis. Importantly, systematically elucidate role medicinal plants active metabolites stroke, providing insights perspectives drugs

Language: Английский

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Genome-wide profiling of WRKY genes involved in flavonoid biosynthesis in Erigeron breviscapus

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: June 3, 2024

The transcription factors of WRKY genes play essential roles in plant growth, stress responses, and metabolite biosynthesis. Erigeron breviscapus , a traditional Chinese herb, is abundant flavonoids has been used for centuries to treat cardiovascular cerebrovascular diseases. However, the that regulate flavonoid biosynthesis E. remain unknown. In this study, total 75 EbWRKY were predicted through comprehensive genome-wide characterization chromosomal localization each gene was investigated. RNA sequencing revealed transient responses 74 exogenous abscisic acid (ABA), salicylic (SA), gibberellin 3 (GA3) after 4 h treatment. contrast, expression key structural involved increased GA3 content metabolites leaves significantly at 12 h. qRT-PCR results showed patterns EbWRKY11 EbWRKY30 EbWRKY31 EbWRKY36 EbWRKY44 exhibited high degree similarity 11 Protein-DNA interactions performed between scutellarin candidate WRKYs . result F7GAT interacts with EbWRKY11, EbWRKY36, EbWRKY44, while EbF6H self-activation function. This study provides information on regulatory control network accumulation mechanisms, offering valuable insights breeding varieties enhanced content.

Language: Английский

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Research progress of traditional Chinese medicine in the treatment of ischemic stroke by regulating mitochondrial dysfunction

Life Sciences, Journal Year: 2024, Volume and Issue: 357, P. 123045 - 123045

Published: Sept. 7, 2024

Language: Английский

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