Identification, biogenesis, function, and mechanism of action of circular RNAs in plants

Plant Communications, Journal Year: 2022, Volume and Issue: 4(1), P. 100430 - 100430

Published: Sept. 7, 2022

Circular RNAs (circRNAs) are a class of single-stranded, closed RNA molecules with unique functions that ubiquitously expressed in all eukaryotes. The biogenesis circRNAs is regulated by specific cis-acting elements and trans-acting factors humans animals. mainly exert their biological acting as microRNA sponges, forming R-loops, interacting RNA-binding proteins, or being translated into polypeptides proteins human animal cells. Genome-wide identification has been performed multiple plant species, the results suggest abundant plants. There emerging compelling evidence to play essential roles during growth development well responses biotic abiotic stress. However, compared recent advances systems, most plants unclear at present. Here we review identification, biogenesis, function, mechanism action circRNAs, which will provide fundamental understanding characteristics complexity

Language: Английский

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Non-coding RNAs as therapeutic targets in cancer and its clinical application

Journal of Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 14(7), P. 100947 - 100947

Published: Feb. 8, 2024

Cancer genomics has led to the discovery of numerous oncogenes and tumor suppressor genes that play critical roles in cancer development progression. Oncogenes promote cell growth proliferation, whereas inhibit division. The dysregulation these can lead cancer. Recent studies have focused on non-coding RNAs (ncRNAs), including circular RNA (circRNA), long (lncRNA), microRNA (miRNA), as therapeutic targets for In this article, we discuss ncRNAs associated with different types their potential targets. Here, highlight mechanisms action clinical applications treatment. Understanding molecular underlying identifying specific are essential steps towards effective treatments.

Language: Английский

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The Role of Circular RNA for Early Diagnosis and Improved Management of Patients with Cardiovascular Diseases

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2986 - 2986

Published: March 4, 2024

Cardiovascular diseases (CVDs) are responsible for approximately 17.9 million deaths every year. There is growing evidence that circular RNAs (circRNAs) may play a significant role in the early diagnosis and treatment of cardiovascular diseases. As regulatory molecules, regulate gene expression, interact with proteins miRNAs, translated into key wide variety biological processes, including division proliferation cells, as well growth development individuals. An overview properties, expression profiles, classification, functions circRNAs presented here, along an explanation their implications heart failure, hypertension, ischemia/reperfusion injury, myocardial infarction, cardiomyopathies, atherosclerosis, arrhythmia.

Language: Английский

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Sequestration of DBR1 to stress granules promotes lariat intronic RNAs accumulation for heat-stress tolerance

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 3, 2024

Heat stress (HS) poses a significant challenge to plant survival, necessitating sophisticated molecular mechanisms maintain cellular homeostasis. Here, we identify SICKLE (SIC) as key modulator of HS responses in Arabidopsis (Arabidopsis thaliana). SIC is required for the sequestration RNA DEBRANCHING ENZYME 1 (DBR1), rate-limiting enzyme lariat intronic (lariRNA) decay, into granules (SGs). The DBR1 by enhances accumulation lariRNAs, branched circular RNAs derived from excised introns during pre-mRNA splicing, which turn promote transcription their parental genes. Our findings further demonstrate that SIC-mediated SGs crucial tolerance, deletion N-terminus (SIC

Language: Английский

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Comprehensive non-coding RNA analysis reveals specific lncRNA/circRNA–miRNA–mRNA regulatory networks in the cotton response to drought stress

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 126558 - 126558

Published: Sept. 1, 2023

Language: Английский

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Designer antisense circRNAGFP reduces GFP abundance in Arabidopsis protoplasts in a sequence-specific manner, independent of RNAi pathways

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Circular RNAs (circRNAs) are single-stranded RNA molecules characterised by their covalently closed structure and emerging as key regulators of cellular processes in mammals, including gene expression, protein function immune responses. Recent evidence suggests that circRNAs also play significant roles plants, influencing development, nutrition, biotic stress resistance, abiotic tolerance. However, the potential to modulate target abundance plants remains largely unexplored. In this study, we investigated designer using Arabidopsis a model system. We demonstrate treatment with 50 nt circRNA_GFP, containing 30 GFP antisense sequence stretch, results reduced reporter dose- sequence-dependent manner. Notably, open isoform circRNA_GFP had little effect on abundance, indicating importance circular structure. Additionally, mutants defective interference (RNAi), suggesting circRNA activity is independent RNAi pathway. show circRNA, unlike dsRNA, does not induce pattern-triggered immunity (PTI) plants. Findings proof-of-principle study together crucial first steps understanding versatile tools for modulating expression offer exciting prospects application agronomy, particularly enhancing crop traits through metabolic pathway manipulation.

Language: Английский

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Interventional effect of hesperetin on N-methyl-N’-nitro-N-nitrosoguanidine-induced exosomal circ008274 in affecting normal cells to promote gastric carcinogenesis

World Journal of Gastroenterology, Journal Year: 2025, Volume and Issue: 31(16)

Published: April 25, 2025

Hesperetin, a flavonoid predominantly present in citrus fruits, exhibits significant intervention effects on both the initiation and progression of gastric cancer. However, specific mechanisms underlying this effect remain unclear. To investigate interventional role hesperetin N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced exosomes inducing carcinogenesis. Bioinformatics technology was used to identify critical molecular components hesperetin-mediated inhibition MNNG induced carcinogenesis through exosomal circular RNA. Biological experiments were conducted validate these findings. Exosomes derived from TGES-1 cells (TGES-1-EX) significantly enhanced proliferation, migration, invasion, epithelial-mesenchymal transition (EMT), stemness GES-1 cells. The oncogenic potential TGES-1-EX diminished following pretreatment. with overexpressed or knocked down circ0008274 extracted treated combination alone. Our investigation revealed that exerted inhibitory MNNG-induced by circ0008274. prediction identified microRNA (miR)-526b-5p as miRNA binding Functional demonstrated may mediate its cancer targeting miR-526b-5p promoted EMT, stem cell-like characteristics miR-526b-5p-mediated regulatory mechanisms. Hesperetin an process, particularly modulation interaction miR-526b-5p.

Language: Английский

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Exploring the Potential and Advancements of Circular RNA Therapeutics

Exploration, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

ABSTRACT Messenger RNA (mRNA) technology is revolutionizing the pharmaceutical industry owing to its superior safety profile, manufacturing capabilities, and potential applications in previously undruggable therapeutic targets. In addition linear mRNA, such as conventional self‐amplifying trans‐amplifying circular mRNA has emerged a promising candidate. Circular (circRNA) class of single‐stranded with covalently closed loop structure that offers enhanced stability compared by resisting degradation from RNases. Recent studies have revolutionized our understanding their biological functions, surpassing notion they are merely byproducts aberrant splicing events. Given remarkable success achieved cancer SARS‐CoV‐2/monkeypox virus (MPXV) vaccines, circRNA being intensively investigated for gene cell therapies. this review, we provide an overview biogenesis mechanisms vivo, along synthesis strategies vitro, while discussing translation regulation quality control processes involved production. Furthermore, explore application scenarios circRNAs.

Language: Английский

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Defense Regulatory Network Associated with circRNA in Rice in Response to Brown Planthopper Infestation

Plants, Journal Year: 2024, Volume and Issue: 13(3), P. 373 - 373

Published: Jan. 26, 2024

The brown planthopper (BPH), Nilaparvata lugens (Stål), a rice-specific pest, has risen to the top of list significant pathogens and insects in recent years. Host plant-mediated resistance is an efficient strategy for BPH control. Nonetheless, rice cultivars succumbed emergence distinct virulent populations. Circular RNAs (circRNAs) play pivotal role regulating plant–environment interactions; however, mechanisms underlying their insect-resistant functions remain largely unexplored. In this study, we conducted extensive genome-wide analysis using high-throughput sequencing explore response circRNAs infestations. We identified total 186 IR56 across two virulence groups: IR-IR56-BPH (referring IR infested by IR56-BPH) IR-TN1-BPH, along with control group (IR-CK) without infestation. Among them, 39 were upregulated, 43 downregulated comparison between IR-CK. Furthermore, IR-CK, 42 exhibited upregulation while showed downregulation. Gene Ontology Kyoto Encyclopedia Genes Genomes enrichment revealed that targets differentially expressed considerably enriched multitude biological processes closely linked assessed 20 randomly selected corresponding expression levels. Moreover, validated regulatory impact on miRNAs mRNAs. These findings have led us construct conceptual model circRNA associated defense network rice, which likely facilitated mediation parental genes competing endogenous RNA (ceRNA) networks. This contributes understanding several extensively studied rice-BPH interactions.

Language: Английский

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Circ_0070934 promotes MGAT3 expression and inhibits epithelial-mesenchymal transition in bronchial epithelial cells by sponging miR-199a-5p

Allergy Asthma and Clinical Immunology, Journal Year: 2024, Volume and Issue: 20(1)

Published: March 23, 2024

Abstract Background Circular RNA (circRNA) has the potential to serve as a crucial regulator in progression of bronchial asthma. The objective this investigation was elucidate functional dynamics circ_0070934/miR-199a-5p/Mannoside acetylglucosaminyltransferase 3 (MGAT3) axis development Methods Circ_0070934, miR-199a-5p and MGAT3 peripheral venous blood 38 asthmatic patients 43 healthy controls were detected by qRT-PCR, expression protein examined ELISA. GSE148000 dataset analyzed for differences MGAT3. BEAS-2B cells transfected with circ_0070934 plasmid small interfering RNA, mimics inhibitors. apoptosis level flow cytometry qRT-PCR Western blot. E-cadherin, N-cadherin, Vimentin Interleukin-4 (IL-4) IL-13 used co-stimulate an airway epithelial cell model. exposed type 2 cytokines (IL-4 IL-13) treated plasmid, binding relationships verified using dual-luciferase reporter assay miRNA pull-down assay. Results down-regulated, up-regulated. And reduced sputum asthma patients. Down-regulating could promote increase epithelial-mesenchymal transition (EMT), effect can be partially reversed down-regulating miR-199a-5p. Circ_0070934 inhibit process mesenchymal induced IL-4 cells. In addition, respectively bind Conclusion findings study indicate that may function competitive endogenous (ceRNA) miR-199a-5p, thereby modulating impacting EMT These results contribute establishment theoretical framework advancing prevention treatment strategies

Language: Английский

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