Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi,
Journal Year:
2023,
Volume and Issue:
13(4), P. 2986 - 2996
Published: Nov. 29, 2023
Bromelain,
extracted
from
the
stem
of
pineapple,
is
a
complex
enzyme
used
for
different
purposes.
Bromelain
supplements
are
often
to
facilitate
digestion,
improve
circulatory
system
and
relieve
arthritis
symptoms
due
its
pain
relief.
However,
in
some
cases
where
there
risk
antibiotic
use
or
bleeding,
bromelain
direct
consumption
pineapple
should
be
limited.
For
this
purpose,
study
was
carried
out
show
mechanism
by
which
interaction
occurs.
Firstly,
molecule
demeclocycline,
minocycline,
tetracycline
antibiotics
were
prepared
UCSF
Chimera
visualizing
program.
The
interactions
monitored
Auto
Dock
Molecular
Modelling
Toolkit
molecular
modeling
free
binding
energies
these
also
calculated
Dock.
According
modelling
results,
interact
with
hydrogen
bonds
hydrophobic
interactions.
These
between
energetically
favorable
based
on
energy
calculations.
ACS Applied Nano Materials,
Journal Year:
2024,
Volume and Issue:
7(9), P. 10144 - 10154
Published: April 23, 2024
Enrofloxacin
(ENR)
is
a
commonly
used
veterinary
antibiotic
and
poses
health
risk
when
present
as
residues
in
food.
This
study
describes
dual-mode
immunochromatographic
test
strip
(DITS)
biosensor
for
the
detection
of
ENR
Magnetic
graphene
oxide
nanoparticles
(MGO-NPs)
are
two-dimensional
magnetic
nanomaterials
with
large
specific
surface
area
fine
biocompatibility,
which
can
better
provide
more
adsorption
points
biological
antibodies.
Gold
(AuNPs)
stimulate
localized
plasmon
resonance
effect
under
laser
irradiation,
will
increase
temperature
to
achieve
photothermal
conversion.
The
conversion
efficiency
MGO-NPs
be
effectively
enhanced
by
combining
AuNPs
form
MGO@Au
nanocomposites
(MGO@Au-NCs).
Then,
MGO@Au-NCs
were
combined
an
monoclonal
antibody
MGO@Au@Ab
probe,
MGO@Au@Ab-DITS
was
developed.
employed
assess
samples
spanning
concentrations
from
0
1000
μg/mL.
Sample
solutions
containing
mixed
solution,
deposited
on
strip's
sample
pad,
analyzed
using
both
colorimetric
methods
employing
808
nm
infrared
imaging
instrument.
results
show
that
sensitivity
mode
sensor
0.0159°C
whereas,
standard
deviation
(SD)
limit
(LOD)
0.058
10.89
μg/mL,
respectively
quantification
(LOQ)
36.31
In
mode,
developed
5.222
au,
SD,
LOD
LOQ
40.13,
23.05
and76.85
respectively.
innovative
system
has
been
applied
detect
food
such
juice
milk,
laying
groundwork
other
drug
Biosensors and Bioelectronics,
Journal Year:
2024,
Volume and Issue:
265, P. 116680 - 116680
Published: Aug. 22, 2024
Aptamers
are
short
oligonucleotides
capable
of
binding
specifically
to
various
targets
(i.e.,
small
molecules,
proteins,
and
whole
cells)
which
have
been
introduced
in
biosensors
such
as
the
electrochemical
aptamer-based
(E-AB)
sensing
platform.
E-AB
sensors
comprised
a
redox-reporter-modified
aptamer
attached
an
electrode
that
undergoes,
upon
target
addition,
binding-induced
change
electron
transfer
rates.
To
date,
faced
limitation
translatability
aptamers
into
platform
presumably
because
sequences
obtained
from
Systematic
Evolution
Ligands
by
Exponential
Enrichment
(SELEX)
typically
long
(>80
nucleotides)
obtaining
structural
information
remains
time
resource
consuming.
In
response,
we
explore
utility
base
truncations
silico
docking
improve
their
sensors.
Here,
first
apply
this
glucose
aptamer,
characterize
solution
using
NMR
methods
guide
design
translate
truncated
variants
biosensors.
We
further
investigated
applicability
truncation
computational
approaches
four
other
systems
(vancomycin,
cocaine,
methotrexate
theophylline)
derived
functional
foresee
our
strategy
will
increase
success
rate
translating
platforms
afford
low-cost
measurements
molecules
directly
undiluted
complex
matrices.
Analytical Methods,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
The
extensive
use
of
antibiotics
poses
significant
public
health
concerns,
including
the
increase
in
drug-resistant
bacteria
and
environmental
pollution,
underscoring
urgent
need
for
rapid,
sensitive,
specific
antibiotic
detection
methods.
Most
current
reviews
on
primarily
focus
categorizing
based
their
types
or
classification
sensors
used,
such
as
electrochemical,
optical,
colorimetric
sensors.
In
contrast,
this
review
proposes
a
novel
systematic
theoretical
framework
using
seven
popular
molecular
recognition
elements-antibodies,
aptamers,
microorganisms,
cells,
peptides,
molecularly
imprinted
polymers
(MIPs),
metal-organic
frameworks
(MOFs)
direct
modalities
briefly
discusses
mechanism
elements
recognition.
Additionally,
it
explores
biosensors
developed
these
elements,
offering
detailed
analysis
strengths
limitations
terms
sensitivity,
specificity,
practicality.
concludes
by
addressing
challenges
future
directions,
providing
comprehensive
perspective
essential
enhancing
food
safety
protecting
health.
