New insights into bioaugmented removal of sulfamethoxazole in sediment microcosms: degradation efficiency, ecological risk and microbial mechanisms

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Feb. 29, 2024

Abstract Background Bioaugmentation has the potential to enhance ability of ecological technology treat sulfonamide-containing wastewater, but low viability exogenous degraders limits their practical application. Understanding mechanism is important and optimize performance bioaugmentation, which requires a multifaceted analysis microbial communities. Here, DNA-stable isotope probing (DNA-SIP) metagenomic were conducted decipher bioaugmentation mechanisms in stabilization pond sediment microcosms inoculated with sulfamethoxazole (SMX)-degrading bacteria ( Pseudomonas sp. M2 or Paenarthrobacter R1). Results The both strains R1, especially strain significantly improved biodegradation rate SMX, its capacity was sustainable within certain cycle (subjected three repeated SMX additions). removal strategy using degrading also abated accumulation transmission risk antibiotic resistance genes (ARGs). Strain inoculation lowered bacterial diversity altered community, while R1 had slight effect on community closely associated indigenous microorganisms. identified as primary SMX-assimilating systems based DNA-SIP analysis. Combining genomic information pure culture evidence, enhanced by directly participating degradation, did it degradation stimulating SMX-degrading activity microorganisms ) community. Conclusions Our findings demonstrate that feasible for clean-up terms efficiency ARG transmission, well impact advantage highlighted.

Language: Английский

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Microbial consortium assembly and functional analysis via isotope labelling and single-cell manipulation of polycyclic aromatic hydrocarbon degraders

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Abstract Soil microbial flora constitutes a highly diverse and complex microbiome on Earth, often challenging to cultivation, with unclear metabolic mechanisms in situ. Here, we present pioneering concept for the situ construction of functional consortia (FMCs) introduce an innovative method creating FMCs by utilizing phenanthrene as model compound elucidate their biodegradation mechanisms. Our methodology involves single-cell identification, sorting, culture microorganisms, resulting formation precise FMC. Through Raman-activated cell sorting–stable-isotope probing, identified isolated phenanthrene-degrading bacterial cells from Achromobacter sp. Pseudomonas sp., achieving controllable based genome-guided cultivation. FMC outperformed conventionally designed when tested real soil, indicating its superior degradation capacity. We revealed that microorganisms high efficiency through conventional methods may exhibit pollutant tolerance but lack actual ability natural environments. This finding highlights potential construct thorough elucidation degraders, thereby sustained efficient degradation. Single-cell sequencing linked degraders genes pathways, providing insights regarding FMCs. The consortium comprising pathways might offer distinct advantages enhancing efficiency, such division labour cooperation or communication among species. approach underscores importance situ, precision isolation, cultivation comprehensive analysis resource exploration, which can extend investigate MFCs archaea fungi, clarifying element recycling transformation real-world ecosystems.

Language: Английский

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Unveiling the synergistic mechanism of autochthonous fungal bioaugmentation and ammonium nitrogen biostimulation for enhanced phenanthrene degradation in oil-contaminated soils

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 465, P. 133293 - 133293

Published: Dec. 19, 2023

Language: Английский

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New mechanisms of biochar-assisted vermicomposting by recognizing different active di-(2-ethylhexyl) phthalate (DEHP) degraders across pedosphere, charosphere and intestinal sphere

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 458, P. 131990 - 131990

Published: July 4, 2023

Language: Английский

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Strategies for remediation of polycyclic aromatic hydrocarbons in contaminated soil: A systematic review and bibliometric analysis

Applied Soil Ecology, Journal Year: 2024, Volume and Issue: 204, P. 105688 - 105688

Published: Oct. 16, 2024

Language: Английский

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Determination of soil phenanthrene degradation through a fungal–bacterial consortium

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(6)

Published: May 16, 2024

Fungal-bacterial consortia enhance organic pollutant removal, but the underlying mechanisms are unclear. We used stable isotope probing (SIP) to explore mechanism of bioaugmentation involved in polycyclic aromatic hydrocarbon (PAH) biodegradation petroleum-contaminated soil by introducing indigenous fungal strain A

Language: Английский

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Metabolic Characterization and Geochemical Drivers of Active Hydrocarbon‐Degrading Microorganisms

Journal of Geophysical Research Biogeosciences, Journal Year: 2024, Volume and Issue: 129(6)

Published: June 1, 2024

Abstract Understanding the metabolic characteristics and controlled geochemical factors of functional microorganisms in petroleum‐contaminated areas at different locations is pivotal for enhancing pollutant removal strategies. To address existing research gap this domain, we employed stable‐isotope‐probing (SIP) with multi‐isotope labeling substrates, combined 16S amplicon sequencing, metagenomic factor analysis. Utilizing n ‐hexadecane phenanthrene as model compounds, our study revealed location‐specific differences composition microorganisms. Despite these variances, key players such Pseudomonas , Marinobacter Alcanivorax Ochrobactrum Sphingomonas consistently emerged active degraders and/or phenanthrene. Several genera, including Nitriliruptoraceae Sphingobacterium demonstrated versatility by effectively degrading both contaminants. SIP‐metagenomic binning facilitated acquisition genomes from degraders, sp., Shinella sp. This enabled a comprehensive analysis petroleum hydrocarbon degradation pathways genes, encompassing PAH dioxygenase alkB phthalate, salicylate‐related pathways. Environmental variation partitioning that oil pollution significantly influences microbial community (12%), followed available potassium nitrogen. Geochemical parameters geographic location independently explained 14% 21% total variations, respectively. Intriguingly, more than half (51%) structure remains unexplained, possibly due to unmeasured environmental variables. Our contributes valuable insights into situ bioremediation mechanism soil, elucidating influencing structures across locations. These findings provide vital theoretical reference regulation diverse contexts.

Language: Английский

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Synergism of endophytic microbiota and plants promotes the removal of polycyclic aromatic hydrocarbons from the Alfalfa rhizosphere

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 478, P. 135513 - 135513

Published: Aug. 20, 2024

Language: Английский

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Bioremediation of petroleum-contaminated soil based on both toxicity risk control and hydrocarbon removal—progress and prospect

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(50), P. 59795 - 59818

Published: Oct. 10, 2024

Language: Английский

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Study on factors influencing the transport and transformation of polycyclic aromatic hydrocarbons in soil–groundwater systems

Emerging contaminants, Journal Year: 2025, Volume and Issue: unknown, P. 100472 - 100472

Published: Jan. 1, 2025

Language: Английский

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