Lipid nanoparticle-based mRNA delivery systems for cancer immunotherapy

Nano Convergence, Journal Year: 2023, Volume and Issue: 10(1)

Published: Aug. 7, 2023

Abstract Cancer immunotherapy, which harnesses the power of immune system, has shown immense promise in fight against malignancies. Messenger RNA (mRNA) stands as a versatile instrument this context, with its capacity to encode tumor-associated antigens (TAAs), cell receptors, cytokines, and antibodies. Nevertheless, inherent structural instability mRNA requires development effective delivery systems. Lipid nanoparticles (LNPs) have emerged significant candidates for cancer providing both protection enhanced intracellular efficiency. In review, we offer comprehensive summary recent advancements LNP-based systems, focus on strategies optimizing design mRNA-encoded therapeutics treatment. Furthermore, delve into challenges encountered field contemplate future perspectives, aiming improve safety efficacy immunotherapies. Graphical

Language: Английский

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Recent advances in extracellular vesicle engineering and its applications to regenerative medicine

Biomaterials Research, Journal Year: 2023, Volume and Issue: 27(1)

Published: Feb. 9, 2023

Extracellular vesicles (EVs) are nanosized particles that released from cells and reflect the characteristics of mother cell. Recently, EVs have been used in several types studies across many different fields. In field EV research, multiple cell culture isolation techniques highlighted importance. Various strategies, including exclusive component media, three-dimensional (3D) cultures, hypoxic conditions, proposed for to control function EVs. Ultracentrifugation, ultrafiltration, precipitation, tangential flow filtration (TFF) utilized isolation. Although isolated their own functionalities, researchers trying functionalize by applying various engineering approaches. Gene editing, exogenous, endogenous, hybridization methods four well-known functionalization strategies. engineered through these processes has applied regenerative medicine, kidney diseases, osteoarthritis, rheumatoid arthritis, nervous system-related others. this review, it was focused on approaches applications medicine.

Language: Английский

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Recent Advancement of Bio-Inspired Nanoparticles in Cancer Theragnostic

Journal of Nanotheranostics, Journal Year: 2023, Volume and Issue: 4(3), P. 299 - 322

Published: July 24, 2023

The introduction of cancer therapeutics and nanotechnology has resulted in a paradigm shift from conventional therapy to precision medicine. Nanotechnology, an interdisciplinary field with focus on biomedical applications, holds immense promise bringing about novel approaches for detection, diagnosis, therapy. past decade witnessed significant research material applications related nanoparticles (NPs). NPs differ small-molecule drugs as they possess unique physicochemical characteristics, such large surface-to-volume ratio, enabling them penetrate live cells efficiently. Traditional therapies, chemotherapy, radiation therapy, targeted immunotherapy, have limitations, cytotoxicity, lack specificity, multiple drug resistance, which pose challenges effective treatment. However, nanomaterials properties that enable new therapeutic modalities beyond delivery the fight against cancer. Moreover, (1–100 nm) numerous benefits, biocompatibility, reduced toxicity, excellent stability, enhanced permeability retention effect, precise targeting, making ideal purpose this article is provide consolidated information various bio-inspired aid theranostics.

Language: Английский

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Hybrid hair follicle stem cell extracellular vesicles co-delivering finasteride and gold nanoparticles for androgenetic alopecia treatment

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 373, P. 652 - 666

Published: Aug. 1, 2024

Language: Английский

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M1-polarized macrophage-derived cellular nanovesicle-coated lipid nanoparticles for enhanced cancer treatment through hybridization of gene therapy and cancer immunotherapy

Acta Pharmaceutica Sinica B, Journal Year: 2024, Volume and Issue: 14(7), P. 3169 - 3183

Published: March 7, 2024

Optimum genetic delivery for modulating target genes to diseased tissue is a major obstacle profitable gene therapy. Lipid nanoparticles (LNPs), considered prospective vehicle nucleic acid delivery, have demonstrated efficacy in human use during the COVID-19 pandemic. This study introduces novel biomaterial-based platform, M1-polarized macrophage-derived cellular nanovesicle-coated LNPs (M1-C-LNPs), specifically engineered combined gene-immunotherapy approach against solid tumor. The dual-function system of M1-C-LNPs encapsulates Bcl2-targeting siRNA within and immune-modulating cytokines M1 nanovesicles (M1-NVs), effectively facilitating apoptosis cancer cells without impacting T NK cells, which activate intratumoral immune response promote granule-mediating killing tumor eradication. Enhanced retention was observed upon administration M1-C-LNPs, owing presence adhesion molecules on M1-NVs, thereby contributing superior growth inhibition. These findings represent promising strategy development targeted effective nanoparticle-based genetic-immunotherapy, with significant implications advancing biomaterial therapeutics.

Language: Английский

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Anti-Inflammatory Artificial Extracellular Vesicles with Notable Inhibition of Particulate Matter-Induced Skin Inflammation and Barrier Function Impairment

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(51), P. 59199 - 59208

Published: Nov. 20, 2023

Particulate matter (PM) exposure disrupts the skin barrier, causing cutaneous inflammation that may eventually contribute to development of various diseases. Herein, we introduce anti-inflammatory artificial extracellular vesicles (AEVs) fabricated through cell extrusion using biosurfactant PEGylated mannosylerythritol lipid (P-MEL), hereafter named AEVP-MEL. The P-MEL has abilities with demonstrated efficacy in inhibiting secretion pro-inflammatory mediators. Mechanistically, AEVP-MEL enhanced response by mitogen-activated protein kinase (MAPK) pathway and decreasing release inflammatory mediators such as reactive oxygen species (ROS), cyclooxygenase-2 (COX-2), cytokines human keratinocytes. Moreover, promoted increased expression levels barrier proteins (e.g., involucrin, IVL) water–proteins aquaporin 3, AQP3). In vivo studies revealed repeated PM intact resulted responses, including thickness (hyperkeratosis) mast infiltration. Importantly, our data showed treatment significantly restored immune homeostasis affected PM-induced intrinsic function. This study highlights potential promoting health against its promising implications for prevention PM-related disorders.

Language: Английский

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Fusogenic Lipid Nanovesicles as Multifunctional Immunomodulatory Platforms for Precision Solid Tumor Therapy

Small, Journal Year: 2025, Volume and Issue: unknown

Published: May 12, 2025

Abstract Although immunotherapy demonstrates considerable prospect in overcoming solid tumors, its clinical efficacy is limited by several factors, such as poor tumor immunogenicity, inadequate immune activation, and immunosuppressive microenvironment (TME). To overcome these challenges, a versatile universal modulation platform should be developed, lipid nanovesicles with membrane fusion capabilities (LNV‐Fs) have attracted great attention for this purpose. By mimicking natural processes, LNV‐Fs enable the precise presentation of immunogenic components on cell membranes, effectively activating anti‐tumor surveillance. Similarly, can equip multiple functionalities autologous adoptive effector cells enhanced therapies. Additionally, function vaccines that elicit robust immunity while promoting long‐term memory. Furthermore, different powerful ability reprogramming TME been reported. Given recent advancements absence comprehensive reviews topic, analysis LNV‐F systems, including their structural classifications, mechanisms, applications cancer provided. future prospects LNV‐Fs, particular emphasis artificial intelligence‐assisted design are explored. This review intended to engage researchers from diverse interdisciplinary fields provide valuable insights advancing precision immunotherapy.

Language: Английский

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Hybrid Nanoparticle Engineered with Transforming Growth Factor -β1-Overexpressed Extracellular Vesicle and Cartilage-Targeted Anti-Inflammatory Liposome for Osteoarthritis

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Extracellular vesicles (EVs) possess the characteristics of their parent cells, based on which various studies have actively investigated treatments for diseases using mesenchymal stem cell-derived EVs due to regenerative activity. Furthermore, in recent years, there been significant efforts engineer improve native activities and integrate additional functions. Although both endogenous exogenous methods are used engineering EVs, may pose problem administering substances cells undergoing metabolic changes, can cause potential side effects. In addition, limitation losing beneficial factors inside membrane disruption during processes. Surface modification also impair efficiency presence proteins EV surface. Therefore, this study, a stable efficient method was achieved through ethanol-mediated hybridization functionalized lipid nanoparticles (LNPs) with fusogenic component. During hybridization, internal bioactive targeting moiety were maintained LNPs EVs. The Ab-Hybrid, successfully synthesized nicotinamide-encapsulated Col2A1 antibody-modified liposome Transforming growth factor-β1 (TGF-β1)-overexpressed administered osteoarthritis (OA)-induced rats destabilization medial meniscus surgery. Ultimately, Ab-Hybrid demonstrated excellent chondroprotective anti-inflammatory effects long-lasting properties OA lesions. We anticipate that approach manufacturing hybrid particles will serve as valuable versatile platform technology applicable diseases.

Language: Английский

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Engineering of Cell Derived-Nanovesicle as an Alternative to Exosome Therapy

Tissue Engineering and Regenerative Medicine, Journal Year: 2023, Volume and Issue: 21(1), P. 1 - 19

Published: Dec. 8, 2023

Language: Английский

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Brief Comparison of the Efficacy of Cationic and Anionic Liposomes as Nonviral Delivery Systems

ACS Omega, Journal Year: 2024, Volume and Issue: 9(47), P. 46664 - 46678

Published: Nov. 13, 2024

In recent decades, the development and application of nonviral vectors, such as liposomes lipidic nanoparticles, for gene therapy drug delivery have seen substantial progress. The interest in physicochemical properties structures complexes liposome/DNA liposome/RNA is due to their potential substitute viruses carriers drugs or genetic material into cells with minimal cytotoxicity, which could lead use therapy. Initially, cationic were utilized DNA vectors; subsequently, different molecules, polymers, incorporated enhance transfection efficiency. Additionally, liposome/protein been developed vectors treatment diseases. most relevant internalization pathways these few results obtained using targeted nontargeted are discussed below. high cytotoxicity represents a significant challenge delivery. Anionic offer promising alternative address limitations conventional liposomes, including immune response, short circulation time, low toxicity. This review will discuss advantages novel anionic liposome-based systems that emerged result. advent designs manufacturing techniques has facilitated innovative systems, designated lipid nanoparticles (LNPs), serve highly efficacious regulators system.

Language: Английский

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