BMC Research Notes, Journal Year: 2024, Volume and Issue: 17(1)
Published: Dec. 27, 2024
The increase of multidrug-resistant (MDR) strains Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency carbapenem genes (oxacillinase metallo-beta-lactamase) in A. baumannii.
Language: Английский
BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)
Published: Feb. 4, 2025
Abstract Background Acinetobacter baumannii is an opportunistic bacteria associated primarily with hospital-acquired infections. Its tendency to acquire or donate resistance genes neighboring a major concern. Tetracyclines have shown promise in treating A. infections, but tetracycline growing globally isolates. Objectives The study aimed (1) the prevalence of multidrug-resistant (MDR) infections at Suez Canal University Hospitals, (2) distribution efflux pump AdeA &B , TetA and TetB (3) effect inhibitor (CCCP) on tetracycline-resistant Methods Clinical samples (457) were collected (blood, urine, sputum, ETA, pus, pleural fluid), followed by isolation identification, PCR detection genes, susceptibility its MIC before after treatment (CCCP). Results A total 31 isolates recovered (6.78%). highest rate was from ICU (48.3%) ET aspirate (48.3%). system distributed 100% isolates, whereas AdeB found 93.5% gene 87.1% All MDR showing three more classes antibiotics. 45% showed 4-fold reduction 12.9% 2-fold MIC. Conclusions Efflux important mechanism for among
Language: Английский
Citations
0
Acta Biochimica Polonica, Journal Year: 2025, Volume and Issue: 72
Published: Feb. 27, 2025
Urinary tract infections (UTI) are a significant problem among populations worldwide. It is mainly associated with the increasing incidence of recurrence, complications and drug resistance uropathogens. The aim this study was to demonstrate prevalence pathogens causing urinary infections. material for data obtained from Mazovian Specialized Hospital (M.S.H) in Radom over period 2 years. Urine collected hospitalized patients UTI. Statistical calculations were performed using statistical software. During period, 3,917 underwent microbiological examination urine, almost 15% them found be infected Based on analysis susceptibility most common uropathogens, it shown that caused by Escherichia coli or Klebsiella pneumoniae, others, often show high fluoroquinolones β-lactam antibiotics. Proteus mirabilis strains have been more resistant aminoglycosides than beta-lactams. In case Pseudomonas aeruginisa, predominates. On other hand, UTI Acientobacter baumannii should treated based results testing due multidrug-resistant strains.
Language: Английский
Citations
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Poultry Science, Journal Year: 2024, Volume and Issue: 103(6), P. 103733 - 103733
Published: April 4, 2024
Salmonella is considered one of the most common foodborne pathogens worldwide. The annual number hospitalizations and deaths related to zoonotic salmonellosis, which transmitted from animals humans infects poultry meat, expected be significant. Hence, primary aims this research were isolate characterize species obtained an integrated company identify some virulence, antimicrobial resistance, with a specific concern about colistin resistance genes. A total 635 samples collected various sources in Jordan screened for accompanying their virulence Samples parent stock house drag swabs, broiler farms, premix, cecum at slaughterhouse level, pre-chilling post-chilling stages, final product. detected 3% (6\200) investigated 13.8% (11/80) cloacal swabs 16.9% (11/65) boiler farms 24.4% (11/45) 16.4% (9/55) stage, 37.8% (17/45) stage 53.3% (24/45) product stage. No isolates feed mills (0/20), parents' premix (0/40), or hatcheries (0/40). resistant ciprofloxacin (91.0%), nalidixic acid (86.5%), doxycycline (83.1%), tetracycline sulphamethoxazole-trimethoprim (79.8%) ampicillin (76.4%). Serotyping shows that S. Infantis was predominant serovar, 56.2%. Based on minimum inhibitory concentration (MIC) test, 39.3% (35/89) colistin; however, no mcr genes detected. Among antimicrobial-resistant genes, blaTEM prevalent (88.8%). Furthermore, spvC, ompA, ompF showed highest percentages (97.8%, 97.8%, 96.6%, respectively). In conclusion, found stages company. serotype. Cross-contamination between production highlights importance good hygiene practices. presence patterns present significant challenges public health.
Language: Английский
Citations
1
Published: May 7, 2024
1) Background: carbapenem-resistant Acinetobacter baumannii (CRAB) is an opportunistic Gram-negative pathogen that has a significant role in healthcare-associated infections. Unlike several studies on the antibiotic-resistant epidemiology of A. baumanni, virulence molecular was less studied. This study aimed to investigate CRAB genes and their ability form biofilms, correlate biofilm formation with both; biofilm-encoding carbapenemase-encoding resistance genes. 2) Methods: 107 clinical isolates were collected from two hospitals Jordan between 2018 2019 screened for using PCR. In addition, assessed microtiter plate method. 3) Results: frequencies bap, OmpA, surA, PLD, paaE, basD, traT 99.10%, 98.20%, 95.50%, 89.10%, 86.40%, 8.20%, respectively. Overall, 86.4% tested formers varying degrees; weak (28.2%), moderate (36.4%) strong (21.8%). A relationship found gene (OXA-23 gene) production. 4) Conclusion: best our knowledge, this first inspected highlighted importance improving infection control measures avoid outbreaks.
Language: Английский
Citations
1
Pathogens, Journal Year: 2024, Volume and Issue: 13(12), P. 1049 - 1049
Published: Nov. 28, 2024
The emergence of antibiotic-resistant Acinetobacter baumannii (A. baumannii) is a pressing threat in clinical settings. Colistin currently widely used treatment for multidrug-resistant A. baumannii, serving as the last line defense. However, reports colistin-resistant strains have emerged, underscoring urgent need to develop alternative medications combat these serious pathogens. To resist colistin, has developed several mechanisms. These include loss outer membrane lipopolysaccharides (LPSs) due mutation LPS biosynthetic genes, modification lipid A (a constituent LPSs) structure through addition phosphoethanolamine (PEtN) moieties component by overexpression chromosomal pmrCAB operon genes and eptA gene, or acquisition plasmid-encoded mcr horizontal gene transfer. Other resistance mechanisms involve alterations permeability porins, expulsion colistin efflux pumps, heteroresistance. In response rising researchers various strategies, including antibiotic combination therapy, adjuvants potentiate activity, repurposing existing drugs, antimicrobial peptides, nanotechnology, photodynamic CRISPR/Cas, phage therapy. While many strategies shown promise vitro vivo, further trials are necessary ensure their efficacy widen applications. Ongoing research essential identifying most effective therapeutic manage baumannii. This review explores genetic underlying assesses potential options this challenging pathogen.
Language: Английский
Citations
0
BMC Research Notes, Journal Year: 2024, Volume and Issue: 17(1)
Published: Dec. 27, 2024
The increase of multidrug-resistant (MDR) strains Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency carbapenem genes (oxacillinase metallo-beta-lactamase) in A. baumannii.
Language: Английский
Citations
0