A Design-Build-Test-Learn Cycle to Potentiate Progress in Isoprenoid Engineering of Photosynthetic Microalgae DOI
Xiangyu Li, Chengxiang Lan,

Xinyi Li

et al.

SSRN Electronic Journal, Journal Year: 2022, Volume and Issue: unknown

Published: Jan. 1, 2022

Currently, the generation of isoprenoid factories in microalgae relies on two strategies: 1) enhanced production endogenous isoprenoids; or 2) heterologous terpenes by metabolic engineering. Nevertheless, low titers and productivity are still a feature biotechnology need to be addressed. In this context, we review mechanisms underlying biosynthesis its relationship with central carbon metabolism. Developments microalgal discussed, advocate new approach trends, challenges prospects involved engineering introducing an integrated "design-build-test-learn" cycle. Finally, discuss emerging promising strategies tools for future. This encourages systematic perspective aimed at potentiating progress photosynthetic microalgae.

Language: Английский

Microalgae-derived tocopherols: Biotechnological advances in production and its therapeutic potentials DOI

Udaypal Udaypal,

Rahul Kumar Goswami, Sanjeet Mehariya

et al.

Sustainable Chemistry and Pharmacy, Journal Year: 2024, Volume and Issue: 42, P. 101791 - 101791

Published: Sept. 27, 2024

Language: Английский

Citations

3
A review on design-build-test-learn cycle to potentiate progress in isoprenoid engineering of photosynthetic microalgae DOI
Xiangyu Li, Chengxiang Lan,

Xinyi Li

et al.

Bioresource Technology, Journal Year: 2022, Volume and Issue: 363, P. 127981 - 127981

Published: Sept. 18, 2022

Language: Английский

Citations

13
Expression of fatty acid related gene promotes astaxanthin heterologous production in Chlamydomonas reinhardtii DOI Creative Commons

Jin-peng Sun,

Xuehong Wei,

Xiaomei Cong

et al.

Frontiers in Nutrition, Journal Year: 2023, Volume and Issue: 10

Published: March 20, 2023

Natural astaxanthin is a high-value ketone carotenoid mainly derived from Haematococcus pluvialis , which an excellent antioxidant for human consumption. To study the role of lipids in accumulation astaxanthin, H. -derived synthesis pathway genes (β-carotene ketolase gene, BKT and β-carotene hydroxylase BCH ) fatty acid elongation gene (mitochondrial trans-2-enoyl-coa reductase MECR were heterologously co-expressed C. reinhardtii . Zeaxanthin, precursor synthesis, was significantly increased after expressed. In contrast, α-carotene that competes with lycopene decreased significantly. This redistribution carbon flow conducive to astaxanthin. addition, transformant only expressed metabolism related ( would lead increase total lipid, decrease monounsaturated acids polyunsaturated acids. On this basis, expression further relative content different also changed. The algal strains transformed nearly 50% higher than wild type. transformants expressing long-chain by 227.5%. study, production model similar combining constructed results suggest indeed linked regulation metabolism, link may involve type dynamics ester cells. strategy promoting has potential achieve efficient

Language: Английский

Citations

8
Proteomic and metabolomic revealed the effect of shading treatment on cigar tobacco DOI Creative Commons

Tongjing Yan,

Bin Cai,

F. D. Li

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: July 19, 2024

Shading or low light conditions are essential cultivation techniques for cigar wrapper tobacco leaves production, yet their impact on protein and metabolic regulatory networks is not well understood. In this study, we integrated proteomic metabolomic analyses to uncover the potential molecular mechanisms affecting under shading treatment. Our findings include: (1) Identification of 780 significantly differentially expressed proteins (DEPs) in leaves, comprising 560 up-regulated 220 down-regulated proteins, predominantly located chloroplast, cytoplasm, nucleus, collectively accounting 50.01%. (2) Discovery 254 metabolites (DEMs), including 148 106 metabolites. (3) KEGG pathway enrichment analysis revealed that mevalonate (MVA) within ‘Terpenoid backbone biosynthesis’ was inhibited, leading a down-regulation ‘Sesquiterpenoid triterpenoid biosynthesis’. Conversely, 2-C-methyl-D-erythritol 4-phosphate (MEP) enhanced, resulting an up-regulation ‘Monoterpenoid biosynthesis’, ‘Diterpenoid ‘Carotenoid thereby promoting synthesis terpenoids such as carotenoids chlorophylls. Simultaneously, Calvin cycle ‘Carbon fixation photosynthetic organisms’ amplified, increasing efficiency. These results suggest conditions, optimizes efficiency by reconfiguring its energy metabolism terpenoid biosynthesis. This study contributes valuable insights into analyses, paving way future functional studies plant responses light.

Language: Английский

Citations

1
A Design-Build-Test-Learn Cycle to Potentiate Progress in Isoprenoid Engineering of Photosynthetic Microalgae DOI
Xiangyu Li, Chengxiang Lan,

Xinyi Li

et al.

SSRN Electronic Journal, Journal Year: 2022, Volume and Issue: unknown

Published: Jan. 1, 2022

Currently, the generation of isoprenoid factories in microalgae relies on two strategies: 1) enhanced production endogenous isoprenoids; or 2) heterologous terpenes by metabolic engineering. Nevertheless, low titers and productivity are still a feature biotechnology need to be addressed. In this context, we review mechanisms underlying biosynthesis its relationship with central carbon metabolism. Developments microalgal discussed, advocate new approach trends, challenges prospects involved engineering introducing an integrated "design-build-test-learn" cycle. Finally, discuss emerging promising strategies tools for future. This encourages systematic perspective aimed at potentiating progress photosynthetic microalgae.

Language: Английский

Citations

0