Moderate static magnetic field promotes fracture healing and regulates iron metabolism in mice DOI Creative Commons

Shenghang Wang,

Yuetong Liu,

Chenge Lou

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: July 10, 2023

Abstract Background Fracture is a prevalent orthopedic disease. Static magnetic fields (SMFs) are known to aid in maintaining bone health, but the effect and mechanism on fracture still unclear. This study aim investigate of moderate static (MMFs) structure metabolism during healing. Methods Eight-week-old male C57BL/6J mice were fractured at tibia, following treatment under geomagnetic field (GMF) MMF. The microarchitecture mechanical properties examined by micro-computed tomography (Micro-CT), 3-point bending. endochondral ossification remodeling evaluated histomorphometric serum biochemical assay. Additionally, atomic absorption spectroscopy ELISA used detect MMF iron mice. Results exposure increased mineral density (BMD), volume per tissue (BV/TV), properties, proportion mineralized matrix callus, reduced cartilage callus area. Meanwhile, number osteoblasts 14th day osteoclasts 28th Furthermore, PINP OCN levels, TRAP-5b β-CTX levels serum. liver callus’s content ferritin, while increasing hepcidin concentration. Conclusions could accelerate healing via promote formation, regulating systemic investigation indicates that be as physical therapy for fractures.

Language: Английский

Moderate static magnetic field regulates iron metabolism and salvage bone loss caused by iron accumulation DOI Creative Commons

Chenxiao Zhen,

Shenghang Wang,

Jiancheng Yang

et al.

Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 50, P. 144 - 157

Published: Jan. 1, 2025

Clinical studies, epidemiological investigations and animal experiments have demonstrated that iron overload lead to bone loss, especially postmenopausal osteoporosis. As a physiotherapy tool, electromagnetic fields already used in clinical treatment of osteoporosis participates remodeling by affecting the metabolism organisms. an field with constant magnetic flux density direction, mechanism static (SMF) regulating remains unclear. Therefore, aim this study was investigate effects moderate (MMF) on HAMP-deficient mouse models, elucidate underlying mechanisms. Firstly, MMF 22 participants were evaluated comparing changes mineral (BMD) serum ferritin before after treatment. Secondly, 10-week-old male C57BL/6 HAMP +/+ -/- mice randomly divided into four groups, namely GMF-HAMP group MMF-HAMP group, (n = 8/group). The MMF-treated exposed daily MMF, while remaining geomagnetic (GMF) for 8 weeks. BMD scanned tissues collected mechanical, structural histological analysis. In addition, analysis tissue content regulation systemic MMF. Finally, differentiation primary macrophages osteoblasts vitro. trial, decreased levels patients, which negatively correlated lumbar BMD. vivo, results showed accompanied overload, along reduced vertebra mass quality. improved mass, microstructure biomechanical properties vertebrae mice. vitro, number osteoclasts mice, promoted osteoblast activating Wnt/β-catenin signaling pathway. Further, also ion conversion enhanced antioxidant system These data suggested could regulate salvage loss caused accumulation. trial laboratory intervention has protective effect disorders. Translational potential article: This feasibility effectiveness demonstrating first time can reduce inherited abnormalities. It plays important role disorders or as alternative therapy ameliorate

Language: Английский

Citations

3
Signalling interaction between β‐catenin and other signalling molecules during osteoarthritis development DOI Creative Commons
Jing Feng, Qing Zhang, Feifei Pu

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(6)

Published: Jan. 10, 2024

Abstract Osteoarthritis (OA) is the most prevalent disorder of synovial joint affecting multiple joints. In past decade, we have witnessed conceptual switch OA pathogenesis from a ‘wear and tear’ disease to entire joint. Extensive studies been conducted understand underlying mechanisms using genetic mouse models ex vivo tissues derived individuals with OA. These revealed that signalling pathways are involved in development, including canonical Wnt/β‐catenin its interaction other pathways, such as transforming growth factor β (TGF‐β), bone morphogenic protein (BMP), Indian Hedgehog (Ihh), nuclear κB (NF‐κB), fibroblast (FGF), Notch. The identification currently underway specific molecule(s) key pathway(s) playing decisive role development need be evaluated. This review will focus on recent progresses understanding critical β‐catenin TGF‐β, BMP, Notch, Ihh, NF‐κB, FGF. Understanding these novel insights into integration complex gene regulatory network during help us identify pathway leading discovery therapeutic strategies for intervention.

Language: Английский

Citations

9
Differentiation of stem cells into chondrocytes and their potential clinical application in cartilage regeneration DOI
Julia Cieśla, Marcin Tomsia

Histochemistry and Cell Biology, Journal Year: 2025, Volume and Issue: 163(1)

Published: Jan. 25, 2025

Language: Английский

Citations

1
Dynamic Proteinaceous Hydrogel Enables In‐Situ Recruitment of Endogenous TGF‐β1 and Stem Cells for Cartilage Regeneration DOI
Qianping Guo,

Weiling Yin,

Huan Wang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: March 19, 2024

Abstract Articular cartilage is a tissue with relatively poor self‐regeneration capacity due to insufficient blood vessels and chondrocytes in the region. Biomaterial‐assisted engineering has shown great potential regeneration. However, there are still many worries over uses of exogenous growth factors, stem cells scaffolds. To address these concerns, here dynamic proteinaceous hydrogel self‐recruiting ability cartilage‐inducing factor for situ regeneration reported. The (Pep‐GelSH) prepared by using thiol‐modified gelatin thiol‐capped TGF‐β1‐affinity peptide through Au‐S coordination. injectability self‐recovery Pep‐GelSH enabled not only minimally invasive implantation but also adaptability scaffold irregular defect shapes. Meanwhile, showed improved adherence host allowed quick infiltration cells. More importantly, significantly enhanced local enrichment endogenous TGF‐β1 led recruitment vivo. After implantation, triggered innate repair defects successively promoting recruitment, differentiation, resulting chondrogenesis repair. Therefore, study this work may provide feasible promising approach

Language: Английский

Citations

8
A review on external physical stimuli with biomaterials for bone repair DOI
T. David Luo, Bowen Tan, Jinfeng Liao

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 153749 - 153749

Published: July 4, 2024

Language: Английский

Citations

8
The emerging role of Piezo1 in the musculoskeletal system and disease DOI Creative Commons
Lei Lei,

Zhenkang Wen,

Mingde Cao

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(10), P. 3963 - 3983

Published: Jan. 1, 2024

Piezo1, a mechanosensitive ion channel, has emerged as key player in translating mechanical stimuli into biological signaling. Its involvement extends beyond physiological and pathological processes such lymphatic vessel development, axon growth, vascular immunoregulation, blood pressure regulation. The musculoskeletal system, responsible for structural support, movement, homeostasis, recently attracted attention regarding the significance of Piezo1. This review aims to provide comprehensive summary current research on Piezo1 highlighting its impact bone formation, myogenesis, chondrogenesis, intervertebral disc tendon matrix cross-linking, physical activity. Additionally, we explore potential targeting therapeutic approach disorders, including osteoporosis, muscle atrophy, degeneration, osteoarthritis.

Language: Английский

Citations

5
Bioactive Materials That Promote the Homing of Endogenous Mesenchymal Stem Cells to Improve Wound Healing DOI Creative Commons

Ziwei Jiang,

Lianglong Chen, Lei Huang

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 7751 - 7773

Published: July 1, 2024

Abstract: Endogenous stem cell homing refers to the transport of endogenous mesenchymal cells (MSCs) damaged tissue. The paradigm using well-designed biomaterials induce resident home in injured site while coordinating their behavior and function promote tissue regeneration is known as regenerative medicine (ERM). ERM a promising new avenue therapy research, it involves mobilizing for principal means through which achieve it. Comprehending how grasp influencing factors essential understanding design engineering. This review summarizes process MSC homing, process, analyses studies interest field skin repair, explores integration promotion strategies with cellular therapies details engineering that can be used modulate cells. In addition providing more systematic theories ideas improved materials this provides perspectives explore complex remodeling enhance rational biomaterial scaffolds guide strategies. Keywords: situ regeneration, biomimetic design, wound healing, bioactive

Language: Английский

Citations

5
The role of mechanically sensitive ion channel Piezo1 in bone remodeling DOI Creative Commons

Yugui Du,

Bowen Xu,

Quiying Li

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Feb. 8, 2024

Piezo1 (2010) was identified as a mechanically activated cation channel capable of sensing various physical forces, such tension, osmotic pressure, and shear force. mediates mechanosensory transduction in different organs tissues, including its role maintaining bone homeostasis. This review aimed to summarize the function possible mechanism mechanical receptor cells tissue. We found that it is potential therapeutic target for treatment diseases.

Language: Английский

Citations

4
Remote actuation and on-demand activation of biomaterials pre-incorporated with physical cues for bone repair DOI Creative Commons

Xueping Kong,

Tianyi Zheng,

Zhaoyi Wang

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(11), P. 4438 - 4461

Published: Jan. 1, 2024

The high incidence of bone defect-related diseases caused by trauma, infection, and tumor resection has greatly stimulated research in the field regeneration. Generally, healing is a long complicated process wherein manipulating biological activity interventional scaffolds to support long-term regeneration significant for treating bone-related diseases. It been reported that some physical cues can act as growth factor substitutes promote osteogenesis through continuous activation endogenous signaling pathways. This review focuses on latest progress repair remote actuation on-demand biomaterials pre-incorporated with (heat, electricity, magnetism). As an alternative method treat defects, show many advantages, including effectiveness, noninvasiveness, manipulation. First, we introduce impact different potential internal regulatory mechanisms. Subsequently, mediate various their respective characteristics are summarized. Additionally, challenges discussed, aiming provide new insights suggestions developing intelligent defects clinical translation.

Language: Английский

Citations

4
A chondroitin sulphate hydrogel with sustained release of SDF-1α for extensive cartilage defect repair through induction of cell homing and promotion of chondrogenesis DOI
Yuezhou Wu, Zhuocheng Lyu, Fei Hu

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(35), P. 8672 - 8687

Published: Jan. 1, 2024

Extensive cartilage damage is a significant challenge in orthopedics. This study introduces novel P-SCMA hydrogel that promotes cell adhesion, proliferation, homing, and chondrogenesis, offering promising solution for effective repair.

Language: Английский

Citations

4