Журнал аналитической химии, Journal Year: 2024, Volume and Issue: 79(6), P. 639 - 652
Published: Dec. 22, 2024
Language: Английский
Журнал аналитической химии, Journal Year: 2025, Volume and Issue: 79(7), P. 733 - 745
Published: Jan. 6, 2025
Language: Английский
Citations
0
Current Research in Green and Sustainable Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 100446 - 100446
Published: Jan. 1, 2025
Language: Английский
Citations
0
The Analyst, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Recent progress in the electrochemiluminescene (ECL) detection of small molecule drugs focused on innovations ECL luminophores, electrode materials, and recognition materials.
Language: Английский
Citations
0
Measurement, Journal Year: 2025, Volume and Issue: unknown, P. 117517 - 117517
Published: April 1, 2025
Language: Английский
Citations
0
Molecules, Journal Year: 2023, Volume and Issue: 28(14), P. 5317 - 5317
Published: July 10, 2023
The interactions of dsDNA with new targeted drug delivery derivatives doxorubicin (DOX), such as DOX embedded into phospholipid nanoparticles (NPhs) and the NGR peptide-modified NPhs were studied electrochemically by differential pulse voltammetry technique. Screen-printed electrodes (SPEs), modified stable fine dispersions carbon nanotubes (CNTs), used for quantitative electrochemical investigations direct oxidation guanine, adenine, thymine heterocyclic bases dsDNA, their changes in presence nanoderivatives. Analysing shifts peak potentials nucleobases drug, we have shown that peptide changed mode interaction DNA-drug complexes from intercalative to electrostatic. Binding constants (Kb) calculated accordance signals. Based on our experiments, proven surface modification a system dramatically mechanism genetic material. DNA-mediated toxicity was based concentration-dependent "response" influence. DOX, DOX-loaded (NPhs), addressed moderately toxic concentration range 0.5-290 µM.
Language: Английский
Citations
9
Heliyon, Journal Year: 2024, Volume and Issue: 10(3), P. e25602 - e25602
Published: Feb. 1, 2024
The aim of this work was to develop an electrochemical approach for the analysis DNA degradation and fragmentation in apoptotic cells. damage is considered one major causes human diseases. We analyzed cleavage processes circular plasmid pTagGFP2-N calf thymus DNA, which were exposed restriction endonucleases (the BstMC I AluB nonspecific endonuclease I). Genomic from leukemia K562 cell line used as a marker early late (mature) stages apoptosis. Registration direct oxidation nucleobases molecules subjected or apoptosis proposed detection these biochemical events. Label-free differential pulse voltammetry (DPV) has been measure activities using carbon nanotube-modified electrodes. present DPV technique provides promising platform high-throughput screening hydrolases registering efficiency processes. comparative its native supercoiled state plasmids restricted 4 23 parts revealed significant differences their behavior. Electrochemical fully confirmed by means traditional methods registration process, such gel electrophoresis flow cytometry.
Language: Английский
Citations
3
Processes, Journal Year: 2023, Volume and Issue: 11(3), P. 922 - 922
Published: March 17, 2023
In the present study, electrochemical behavior of antiviral drug umifenovir (Umi) and encapsulated in phospholipids micelles (nanosome/umifenovir, NUmi) were investigated for first time on screen-printed electrodes modified by carbon nanotubes. We have shown that Umi can be electro oxidized around potential +0.4 V concentration range 50–500 µM (R2 = 0.992). Non-overlapping signatures DNA (10–150 µM) permit to register interaction between (or micelles), purine, pyrimidine heterocyclic bases separately. The type is most likely via electrostatic interactions groove binding drug-DNA formed complex, as was revealed based values constants Kb cathodic shifts oxidation potentials with increasing or NUmi concentration. negative Gibbs free energy (ΔG) all nucleobases confirm process spontaneity. This study one presenting effect dsDNA a target pharmacogenomics.
Language: Английский
Citations
6
Sensors, Journal Year: 2023, Volume and Issue: 23(19), P. 8242 - 8242
Published: Oct. 4, 2023
An electrochemically active polymer, polythionine (PTN), was synthesized in natural deep eutectic solvent (NADES) via multiple potential scans and characterized using cyclic voltammetry electrochemical impedance spectroscopy (EIS). NADES consists of citric acid monohydrate, glucose, water mixed the molar ratio 1:1:6. Electrodeposited PTN film then applied for electrostatic accumulation DNA from salmon sperm used sensitive detection anticancer drug epirubicin. Its reaction with resulted regular changes EIS parameters that made it possible to determine 1.0-100 µM epirubicin limit (LOD) 0.3 µM. The sensor developed successfully spiked samples artificial urine saliva, recovery ranging 90 109%. protocol assembling utilized only one drop reactants performed a minimal number steps. Together simple measurement requiring 100 µL sample, this offers good opportunities further use monitoring level biological samples, which is necessary oncology treatment pharmacokinetics studies new antitumor drugs.
Language: Английский
Citations
5
Biosensors, Journal Year: 2023, Volume and Issue: 13(5), P. 513 - 513
Published: April 30, 2023
Electrochemical DNA sensors are highly demanded for fast and reliable determination of antitumor drugs chemotherapy monitoring. In this work, an impedimetric sensor has been developed on the base a phenylamino derivative phenothiazine (PhTz). A glassy carbon electrode was covered with electrodeposited product PhTz oxidation obtained through multiple scans potential. The addition thiacalix[4]arene derivatives bearing four terminal carboxylic groups in substituents lower rim improved conditions electropolymerization affected performance electrochemical depending configuration macrocyclic core molar ratio molecules reaction medium. Following that, deposition by physical adsorption confirmed atomic force microscopy impedance spectroscopy. redox properties surface layer changed electron transfer resistance presence doxorubicin due to its intercalating helix influencing charge distribution interface. This made it possible determine 3 pM-1 nM 20 min incubation (limit detection 1.0 pM). tested bovine serum protein solution, Ringer-Locke's solution mimicking plasma electrolytes commercial medication (doxorubicin-LANS) showed satisfactory recovery rate 90-105%. could find applications pharmacy medical diagnostics assessment able specifically bind DNA.
Language: Английский
Citations
3
Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 397, P. 134701 - 134701
Published: Sept. 28, 2023
This paper presents a novel biosensor for detecting the active metabolite of irinotecan, SN38, with high selectivity and sensitivity. The utilizes dsDNA decamer (5'-GCGTTGTCGC-3') that selectively interacts SN38. biosensor, consisting screen-printed graphite electrode as recognition layer, demonstrates stability, sensitivity, towards SN38 through electrochemical studies. It offers wide linear range 1-200 nM, comparable to high-performance liquid chromatography (HPLC) method, sensitivity 57 nA/nM detection limit 1.06 nM. Compared bare electrode, exhibits significant 31-fold increase in three orders magnitude lower. Selectivity studies were conducted using electrochemically inactive interferents, including biologically compounds. Bioreceptor interactions chosen interferents examined HPLC nuclear magnetic resonance (NMR). Tests was performed laboratory samples artificial urine spiked disposable based on dsDNA, provides an innovative approach accurately determining levels therapeutic drug concentration range. holds promise alternative method clinical diagnosis monitoring cancer patients undergoing irinotecan treatment.
Language: Английский
Citations
3