Bifidobacterium bifidum Ameliorates DSS-Induced Colitis in Mice by Regulating Microbial Metabolome and Targeting Gut Microbiota

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(24), P. 13593 - 13609

Published: June 5, 2024

Inflammatory bowel disease (IBD) is a recurrent inflammatory condition affecting the gastrointestinal tract, and its clinical treatment remains suboptimal. Probiotics have shown effectiveness in alleviating dextran sulfate sodium salt (DSS)-induced colitis, exhibiting strain-specific anti-inflammatory properties. In this study, we compared therapeutic effects of five strains Bifidobacterium bifidum isolated from healthy adult feces on DSS-induced colitis mice. Additionally, investigated underlying mechanisms by examining gut microbiota composition microbial metabolome. Our findings highlighted superior efficacy B. M1-3 to other strains. It significantly improved symptoms, mitigated barrier disruption, reduced colonic inflammation DSS-treated Moreover, analysis revealed that increased abundance diversity microbiota. Specifically, it Muribaculaceae, Lactobacillus, Bacteroides, Enterorhabdus, while decreasing Escherichia-Shigella. Furthermore, our nontargeted metabolomics illustrated had regulatory effect various metabolic pathways, including tyrosine metabolism, lysine degradation, tryptophan metabolism. Importantly, confirmed efficiency was dependent These results are conducive development probiotic products for colitis.

Language: Английский

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Fucoidan alleviated dextran sulfate sodium–induced ulcerative colitis with improved intestinal barrier, reshaped gut microbiota composition, and promoted autophagy in male C57BL/6 mice

Nutrition Research, Journal Year: 2023, Volume and Issue: 122, P. 1 - 18

Published: Nov. 17, 2023

Language: Английский

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Moutai Distiller's grains Polyphenol extracts and rutin alleviate DSS-induced colitis in mice: Modulation of gut microbiota and intestinal barrier function （R2）

Heliyon, Journal Year: 2023, Volume and Issue: 9(11), P. e22186 - e22186

Published: Nov. 1, 2023

Distiller's grains, byproducts of the brewing process, represent a valuable resource for extracting natural phenolic compounds due to their significant global production. This study presents first evidence protective effects Moutai distiller's grain polyphenol extract (MDGP) on dextran sulfate sodium (DSS)-induced colitis in mice. These manifest predominantly through amelioration general indices and histopathological improvements. Utilizing liquid chromatography-high-resolution electrospray ionization mass spectrometry (LC-HR-ESI-MS), main components MDGP were identified as rutin, quercetin, naringenin, dihydroquercetin. Moreover, novel mechanism was elucidated by which primary active component MDGP, alleviates DSS-induced colitis. Assessment intestinal barrier function, microbial sequencing, fecal transplantation, antibiotic depletion experiments revealed that rutin suppresses abundance pathogenic bacteria (Helicobacter, Klebsiella, Veillonella) while promoting proliferation beneficial (Ruminococcus_torques_group, Lachnoclostridium, norank_f__Muribaculaceae). modulation culminates elevated butyric acid concentrations within short-chain fatty acids (SCFAs), amplified integrity tight (ZO-1, occludin) adherent (E-cadherin, β-catenin) junctional complexes, fortified diminished inflammation.This investigation accentuates innovative therapeutic potential its component, assuaging inflammation fortifying mediated microbiota. Such insights potentially elevate prominence grains realm functional food development.

Language: Английский

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Advances and optimization strategies in bacteriophage therapy for treating inflammatory bowel disease

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: May 8, 2024

In the advancement of Inflammatory Bowel Disease (IBD) treatment, existing therapeutic methods exhibit limitations; they do not offer a complete cure for IBD and can trigger adverse side effects. Consequently, exploration novel therapies multifaceted treatment strategies provides patients with broader range options. Within framework IBD, gut microbiota plays pivotal role in disease onset through diverse mechanisms. Bacteriophages, as natural microbial regulators, demonstrate remarkable specificity by accurately identifying eliminating specific pathogens, thus holding promise. Although clinical trials have affirmed safety phage therapy, its efficacy is prone to external influences during storage transport, which may affect infectivity regulatory roles within microbiota. Improving stability precise dosage control bacteriophages—ensuring robustness consistent dosing, targeted delivery infection sites—is crucial. This review thoroughly explores latest developments inherent challenges, focusing on interaction between bacteriophages. It highlights bacteriophages’ potential microbiome modulators offering detailed insights into research bacteriophage encapsulation Particular attention paid functionality various carrier systems, especially regarding their protective properties ability colon-specific delivery. aims provide theoretical foundation using bacteriophages paving way enhanced regulation intestinal

Language: Английский

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Chemical profiling and quality evaluation of Liuweizhiji Gegen‐Sangshen oral liquid by UPLC‐Q‐TOF‐MS and HPLC‐diode array detector fingerprinting

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: 35(4), P. 860 - 872

Published: Feb. 15, 2024

Abstract Introduction Liuweizhiji Gegen‐Sangshen (LGS) oral liquid is a Chinese patent medicine that widely used for the prevention and treatment of alcoholic liver disease in clinical practice. However, chemical complexity LGS has not yet been investigated. Objective The aim this study was to rapidly identify constituents establish quality control method based on fingerprint quantitative analysis. Methodology A comprehensive strategy by combining qualitative analysis ultra‐performance chromatography tandem quadrupole time‐of‐flight mass spectrometry (UPLC‐Q‐TOF‐MS) high‐performance with diode array detection (HPLC‐DAD). Results total 162 components LGS, including 91 flavonoids, 31 organic acids, 20 phenolic compounds, were identified or preliminarily characterized both positive negative ion modes UPLC‐Q‐TOF‐MS results. Of these, 37 confirmed reference standards. In analysis, 23 peaks chosen as common evaluate similarity different batches LGS. Subsequently, rapid quantification optimized validated simultaneous determination multiple markers successfully analyze samples. Conclusion proposed HPLC‐DAD fingerprinting multi‐component demonstrated satisfactory results high efficiency, accuracy, reliability. This can be overall consistency evaluation medicines.

Language: Английский

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Association between gut microbiota and spinal stenosis: a two-sample mendelian randomization study

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: April 19, 2024

Considerable evidence has unveiled a potential correlation between gut microbiota and spinal degenerative diseases. However, only limited studies have reported the direct association stenosis. Hence, in this study, we aimed to clarify relationship using two-sample mendelian randomization (MR) approach.

Language: Английский

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Fingerprint profiling for quality evaluation and the related biological activity analysis of polysaccharides from Liuweizhiji Gegen-Sangshen beverage

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: July 8, 2024

Introduction Liuweizhiji Gegen-Sangshen beverage (LGS) is popular in China, which has been used for alleviating alcohol-mediated discomfort and preventing alcoholic liver disease (ALD). This consisted of six herbal components that are known as functional foods fruits. LGS rich polysaccharides, however, the activity quality evaluation LGS-derived polysaccharides remain unexplored. The purpose this study thus to establish a comprehensive control methodology assessment (LGSP) further explore anti-oxidant, anti-inflammatory well prebiotic effect LGSP. Methods LGSP was extracted, followed by analysis molecular weight distribution, monosaccharide content structural characterization via integrating application high-performance size exclusion chromatography (HPSEC), 1-phenyl-3-methyl-5-pyrazolone-HPLC (PMP-HPLC), fourier transform infrared spectroscopy (FT-IR) nuclear magnetic resonance (NMR) techniques. anti-oxidation determined DPPH, ABTS, hydroxyl radical scavenging capacity total antioxidant capacity. anti-inflammation were assessed on RAW 264.7 cells. growth Lactobacillus, Bifidobacterium bifidum adolescentis evaluated. Results results demonstrated had two distribution peaks, with average weights (6.569 ± 0.12) × 10 4 Da (4.641 0.30) Da. composed 8 monosaccharides, galacturonic acid, glucose rhamnose galactose representing highest molar ratios. Homogalacturonic acid (HG) type rhamnosegalacturonic glycans I (RG-I) α-1,4-glucan present concentration 17.94 0.28 mg/mL. Furthermore, fingerprint combined composition quantification batches there high similarity among batches. Notably, exhibited anti-oxidant inhibited expressions pro-inflammatory factors (TNF-α IL-6) LPS-stimulated In addition, remarkably promoted proliferation probiotics adolescentis, showing good activity. Discussion would be help gain understanding structure–activity relationship LGSP, provide reference bioactive facilitate development unique health products future.

Language: Английский

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Novel selenium-enriched Pichia kudriavzevii as a dietary supplement to alleviate dextran sulfate sodium-induced colitis in mice by modulating the gut microbiota and host metabolism

Food & Function, Journal Year: 2024, Volume and Issue: 15(21), P. 10698 - 10716

Published: Jan. 1, 2024

Inflammatory bowel disease (IBD) poses persistent challenges due to its chronic and recurrent nature, exacerbated by the unsatisfactory outcomes of traditional treatment approaches. In this study, we developed a dietary supplement, selenium-enriched

Language: Английский

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Effect of Dendrobium Officinale on Radioactive Oral Mucositis of Nasopharyngeal Carcinoma and Changes of Oral Microecology

Integrative Cancer Therapies, Journal Year: 2025, Volume and Issue: 24

Published: Jan. 1, 2025

Objective: To observe the clinical efficacy of Dendrobium officinale in treatment radiotherapy-induced oral mucositis nasopharyngeal carcinoma patients, and to explore its regulating effect on immune function microbiota by comparing immune-related factors before after intervention. Methods: We conducted a randomized double-blinded controlled trial Zhejiang Cancer Hospital. Sixty patients with cancer combined were randomly divided into study group control group, 30 cases each The used compound vitamin B12 solution tea drink, simply rinse. When developed (at time 10F radiotherapy), 1 month end salivary flow rate was measured without stimulation evaluate degree efficacy. also detected content EGF saliva IL-10 IL-11 serum, analyzed differences microbial community structure. All consented enrollment. Results: mucosal fraction significantly improved, which better than that group( P < .05). increased compared treatment( There significant difference between flora (Unique OTU counts: 5390 vs 3906), there 5671 5439). After treatment, Erysipelotrichales (Phylum Firmicutes, LDA score = 2.80, .034), Leptotrichiaceae (Fusobacteria,LDA 3.38, .030) Campylobacteraceae (Proteobacteria, 3.35, .026) enriched group. use showed little diversity abundance, but among bacteria genera. Conclusions: is effective mucositis, may be related improvement gland regulation microenvironment. reduce symptoms affecting systemic cellular function. It secretion pro-inflammatory relevant directly changing micro-ecology.

Language: Английский

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Cyanidin-3-glucoside improves cognitive impairment in naturally aging mice by modulating the gut microbiota and activating the ERK/CREB/BDNF pathway

Food Research International, Journal Year: 2025, Volume and Issue: 208, P. 116086 - 116086

Published: Feb. 26, 2025

Language: Английский

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