Drug Development Research,
Journal Year:
2023,
Volume and Issue:
84(7), P. 1496 - 1512
Published: Aug. 11, 2023
A
reliable
and
efficient
in
vitro
model
is
needed
to
screen
drugs
for
Alzheimer's
disease
(AD),
as
many
are
currently
the
developmental
stage.
To
address
this,
we
developed
an
using
amniotic
membrane-derived
mesenchymal
stem
cells
(AM-MSCs)
novel
AD.
We
differentiated
AM-MSCs
into
neurons
degenerated
them
beta
amyloid1-42
(Aß).
then
tested
AD
drugs,
which
commercially
available
such
donepezil,
rivastigmine,
memantine,
citicoline,
two
that
is,
probucol,
anti-hyperlipidaemic
drug,
NMJ-2,
a
cinnamic
acid
analogue
their
potential
protect
against
neurodegeneration.
used
gene
expression
immunofluorescence
staining
assess
neuroprotective
ability
of
these
drugs.
also
measured
reduce
lactate
dehydrogenase,
reactive
oxygen
species,
nitric
oxide
levels,
well
stabilize
mitochondrial
membrane
increase
acetylcholine
(ACh)
levels.
The
reduced
cytotoxicity
oxidative
stress,
stabilized
potential,
restored
ACh
Furthermore,
they
BACE1
expression,
with
concomitant
cholinergic
markers.
This
AM-MSCs-based
AD-like
has
immense
be
accurate
human
alternative
animal
models
testing
large
number
lead
compounds
short
time.
Our
results
suggest
probucol
NMJ-2
may
Aß-induced
Journal of Agricultural and Food Chemistry,
Journal Year:
2023,
Volume and Issue:
71(9), P. 3981 - 3993
Published: Feb. 24, 2023
Overwhelming
evidence
points
to
an
abnormally
active
Wnt/β-catenin
signaling
as
a
key
player
in
colorectal
cancer
(CRC)
pathogenesis.
Ursolic
acid
(UA)
is
pentacyclic
triterpenoid
that
has
been
found
broad
variety
of
fruits,
spices,
and
medicinal
plants.
UA
shown
have
potent
bioactivity
against
cancers,
including
CRC,
with
the
action
mechanism
obscure.
Our
study
tried
learn
more
about
efficacy
on
CRC
its
functional
amid
cascade.
We
determined
SW620
cells
respect
proliferation,
migration,
clonality,
apoptosis,
cell
cycle,
cascade,
assessment
effect
normal
colonic
NCM460
cells.
Also,
effects
tumor
development,
axis
were
evaluated
after
subcutaneous
xenograft
model
was
established
mice.
In
this
work,
we
showed
drastically
suppressed
clonality;
induced
apoptosis;
arrested
cycle
at
G0/G1
phase
cells,
without
influence
accompanied
by
weakened
activity
pathway.
Besides,
markedly
deterred
growth
tumor,
ameliorated
pathological
features,
triggered
tissue,
lessening
Overall,
may
inhibit
malignant
phenotype,
induce
arrest
potentially
attenuating
axis,
providing
insights
into
for
potency
CRC.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 1, 2024
Abstract
Immunotherapy
offers
a
promising
avenue
for
reducing
tumor
metastasis
and
recurrence
but
faces
challenges
from
the
immunosuppressive
microenvironment
(TIME)
restricted
antigen
presentation.
To
address
these
challenges,
this
study
have
developed
an
innovative
approach
utilizing
molybdenum
(Mo)‐doped
Prussian
blue
nanoparticles
coated
with
cancer
cell
membrane
(CCM),
referred
to
as
PMo@CCM.
This
novel
nanoplatform
excels
in
performing
photothermal
therapy
(PTT),
while
Mo
Fe
components
effectively
deplete
glutathione
(GSH)
generate
reactive
oxygen
species
(ROS),
thereby
significantly
enhancing
chemodynamic
(CDT)
remodeling
TIME.
The
synergistic
PTT/CDT
not
only
induces
immunogenic
death
(ICD)
also
facilitates
CCM
coating
further
supplies
antigens
prompts
dendritic
(DC)
maturation.
comprehensive
strategy
markedly
enhances
effectiveness
of
immunotherapy,
evidenced
by
significant
increase
T
activation.
Moreover,
use
programmed
protein
1
antibodies
(anti
PD‐1)
blocks
PD‐1
immune
checkpoint
pathway.
RNA
sequencing
analysis
has
identified
genes
associated
observed
substantial
reduction
growth.
In
conclusion,
PMo@CCM
enables
homologously
targeted
therapy,
guided
magnetic
resonance
imaging
(PTI&MRI),
impeding
progression
both
primary
metastatic
tumors.
Translational Cancer Research,
Journal Year:
2024,
Volume and Issue:
13(1), P. 348 - 362
Published: Jan. 1, 2024
Background:
Although
there
are
many
treatments
for
breast
cancer,
such
as
surgery,
radiotherapy,
chemotherapy,
estrogen
receptor
antagonists,
immune
checkpoint
inhibitors
and
so
on.
However,
safer
more
effective
therapeutic
drugs
cancer
needed.
Sinensetin,
a
drugs,
come
from
citrus
species
medicinal
plants
used
in
traditional
medicine,
while
its
role
underlying
mechanism
remain
unclear.
Our
study
aimed
to
investigate
the
of
sinensetin
cancer.
Methods:
Cell
Counting
Kit-8
(CCK-8)
was
determine
safe
concentration
MCF-10A,
MCF7
MDA-MB-231
cells;
120
μM
subsequent
experiments.
Real
time
polymerase
chain
reaction
(RT-PCR),
Western
blotting,
Terminal
Deoxynucleotidyl
Transferase
mediated
dUTP
Nick-End
Labeling
(TUNEL)
apoptosis
assay,
Transwell
invasion
assay
Clone
formation
were
this
cell
viability,
mRNA
expression,
protein
levels,
apoptosis,
proliferation,
Results:
Herein,
our
results
showed
that
suppressed
viability
promoted
cells.
Treatment
with
µM
24
h
no
significant
toxicity
normal
mammary
decreased
invasion,
epithelial-mesenchymal
transition
(EMT),
downregulated
β-catenin,
lymphatic
enhancing
factor
1
(LEF1),
T-cell
(TCF)
1/TCF7,
TCF3/TCF7L1
expression
The
Wnt
agonist
SKL2001
reversed
inhibitory
effect
on
survival,
metastasis,
EMT.
Sinensetin-induced
downregulation
LEF1,
TCF1/TCF7
upregulated
by
Conclusions:
In
summary,
metastasis
via
inhibition
Wnt/β-catenin
pathway
adverse
effects
confirmed
cells
provided
better
understanding
mechanism.
Frontiers in Cell and Developmental Biology,
Journal Year:
2023,
Volume and Issue:
11
Published: July 10, 2023
Breast
cancer
stem
cells
(BCSCs)
represent
a
distinct
subpopulation
of
with
the
ability
to
self-renewal
and
differentiate
into
phenotypically
diverse
tumor
cells.
The
involvement
CSC
in
treatment
resistance
recurrence
has
been
well
established.
Numerous
studies
have
provided
compelling
evidence
that
is
tightly
regulated
by
specific
signaling
pathways,
which
exert
critical
roles
maintain
an
undifferentiated
phenotype
prevent
differentiation
CSCs.
Signaling
pathways
such
as
Wnt/β-catenin,
NF-κB,
Notch,
Hedgehog,
TGF-β,
Hippo
implicated
promotion
many
normal
Given
pivotal
role
BCSCs
driving
breast
aggressiveness,
targeting
holds
promise
viable
therapeutic
strategy
for
combating
this
disease.
In
review,
we
will
discuss
main
involved
maintenance
BCSC,
while
also
highlighting
current
strategies
employed
disrupt
molecules
associated
stemness.
Oncology Reports,
Journal Year:
2024,
Volume and Issue:
52(1)
Published: June 6, 2024
Ursolic
acid
(UA),
a
pentacyclic
triterpenoid
that
has
been
found
in
broad
variety
of
fruits,
spices
and
medicinal
plants,
various
biological
effects
such
as
reducing
inflammation,
protecting
cells
from
damage,
preserving
brain
function.
However,
its
impact
on
ferroptosis
cancer
stem‑like
remains
unexplored.
The
present
study
investigated
the
effect
UA
MDA‑MB‑231
BT‑549
cell‑derived
triple‑negative
breast
CSCs
(BCSCs)
potential
pathway.
BCSCs
were
demonstrated
by
detection
ferroptosis‑related
indexes
including
intracellular
level
glutathione,
malondialdehyde,
reactive
oxygen
species
iron.
behaviors
analyzed
Cell
Counting
Kit‑8,
stemness
mammosphere
formation
assay.
mechanism
induction
was
explored
reverse
transcription‑quantitative
PCR
western
blotting.
BALB/c‑nude
mice
subcutaneously
injected
with
MDA‑MB‑231‑derived
to
establish
xenograft
models
detect
Biotechnology and Genetic Engineering Reviews,
Journal Year:
2023,
Volume and Issue:
39(2), P. 729 - 759
Published: Jan. 4, 2023
The
world
is
currently
facing
a
global
challenge
against
neoplastic
diseases.
Chemotherapy,
hormonal
therapy,
surgery,
and
radiation
therapy
are
some
approaches
used
to
treat
cancer.
However,
these
treatments
frequently
causing
side
effects
in
patients,
such
as
multidrug
resistance,
fever,
weakness,
allergy,
among
others
effects.
As
result,
current
research
has
focused
on
phytochemical
compounds
isolated
from
plants
deadly
cancers.
Plants
excellent
resources
of
bioactive
molecules,
many
natural
molecules
have
exceptional
anticancer
properties.
They
produce
diverse
derivatives
alkaloids,
terpenoids,
flavonoids,
pigments,
tannins,
which
powerful
activities
various
cancer
cell
lines
animal
models.
Because
their
safety,
eco-friendly,
cost-effective
nature,
communities
recently
molecules.
Ursolic
acid
(UA)
its
derivative
anti-inflammatory,
anticancer,
apoptosis
induction,
anti-carcinogenic,
anti-breast
proliferation
can
improve
the
clinical
management
human
because
it
inhibits
viability
proliferation,
preventing
tumour
angiogenesis
metastatic
activity.
Therefore,
present
article
focuses
numerous
bioactivities
(UA),
inhibit
production,
mechanism
action,
modulation
properties
via
regulating
cellular
processes.
Pharmacology & Therapeutics,
Journal Year:
2021,
Volume and Issue:
223, P. 107800 - 107800
Published: Jan. 8, 2021
Breast
cancer
(BCa)
is
one
of
the
most
prevalent
malignant
tumors
affecting
women's
health
worldwide.
The
recurrence
and
metastasis
BCa
have
made
it
a
long-standing
challenge
to
achieve
remission-persistent
or
disease-undetectable
clinical
outcomes.
Cancer
stem
cells
(CSCs)
possess
ability
self-renew
generate
heterogeneous
tumor
bulk.
existence
CSCs
has
been
found
be
vital
in
initiation,
metastasis,
therapy
resistance,
across
types.
Because
grow
slowly
their
dormant
state,
they
are
insensitive
conventional
chemotherapies;
however,
when
emerge
from
state
become
clinically
evident,
usually
acquire
genetic
traits
that
make
them
resistant
existing
therapies.
Moreover,
also
show
evidence
acquired
drug
resistance
synchrony
with
relapses.
concept
provides
new
treatment
strategy
for
BCa.
In
this
review,
we
highlight
recent
advances
research
on
breast
association
epithelial-mesenchymal
transition
(EMT),
circulating
(CTCs),
plasticity
cells,
microenvironment
(TME),
T-cell
modulatory
protein
PD-L1,
non-coding
RNAs.
On
basis
associated
multiple
dysregulated
biological
processes,
envisage
increased
understanding
disease
sub-classification,
selected
combination
treatment,
molecular
aberration
directed
therapy,
immunotherapy,
CSC
targeting/sensitizing
might
improve
outcome
patients
advanced
We
discuss
novel
perspectives
drugs
therapeutics
purposing
potent
selective
expunging
CSCs.
