Deacetylepoxyazadiradione Derived from Epoxyazadiradione of Neem (Azadirachta indica A. Juss) Fruits Mitigates LPS‐Induced Oxidative Stress and Inflammation in Zebrafish Larvae DOI
Raghul Murugan, Ravi Rajesh, Ajay Guru

et al.

Chemistry & Biodiversity, Journal Year: 2022, Volume and Issue: 19(9)

Published: Aug. 26, 2022

Reactive oxygen species (ROS) produced by cell metabolism have a duplex role in oxidation and inflammation reactions which involve damage or repair responses. Excess ROS production has detrimental effects on the survival of cells. We examined protective effect semi-natural compound NF2 (deacetylepoxyazadiradione), for its activity against free radical-mediated stress inflammatory response to lipopolysaccharide (LPS) using zebrafish larvae. Preliminary antioxidant assays indicated an increase scavenging radicals from than NF1 (Epoxyazadiradione) concentration-dependent manner. Cell cytotoxicity was determined rat myoblast lines (L6), more 95 % viability obtained. Zebrafish developmental toxicity test that is not toxic even at 150 μM. The percentage ROS, lipid peroxidation, nitric oxide apoptosis were reduced significantly treated LPS-stressed number employed macrophages treatment observed neutral red dye-marked macrophage localization images. Relative expression genes larvae after with increased. RT-PCR quantification anti-inflammatory gene decreased relative folds pro-inflammatory cytokines, iNOS increased mitochondrial (GR, GST GPx) LPS stressed NF2. From overall obtained results, it can be concluded oxidative caused LPS.

Language: Английский

Embryonic exposure to butylparaben and propylparaben induced developmental toxicity and triggered anxiety‐like neurobehavioral response associated with oxidative stress and apoptosis in the head of zebrafish larvae DOI
Christy Lite, Ajay Guru, Melita Juliet

et al.

Environmental Toxicology, Journal Year: 2022, Volume and Issue: 37(8), P. 1988 - 2004

Published: April 26, 2022

Parabens are synthetic antimicrobial compounds used as a preservative for extending the shelf life of food, pharmaceutical and cosmetic products. The alkyl chain length paraben esters positively correlates with their property. Hence, long-chain esters, namely butylparaben propylparaben, in combination they have better solubility efficacy. Extensive use parabens has now resulted ubiquitous presence these various human environmental matrices. During early life, exposure to contaminants is known cause oxidative-stress mediated apoptosis developing organs. brain being one high oxygen-consuming, metabolically active lipid-rich organ, it primarily susceptible reactive oxygen species (ROS) lipid peroxidation (LP) induced neuronal cell death. primary impairment cognitive emotional neurobehvioural outcomes neurodegenerative disease was found be associated apoptosis. present study aimed propylparaben's effect on zebrafish during embryonic stages. Besides this, association between alteration anxiety-like neurobehavioral response oxidative stress antioxidant status head region also studied. results showed variation toxic signature left by propylparaben developmental parameters such hatching rate, survival non-lethal malformations time-dependent manner. Data from light-dark preference test trigger behavior larvae. In addition, significant increase intracellular ROS LP levels correlated suppressed enzymes: superoxide dismutases (SOD), catalases (CAT), Glutathione peroxidase (GPx), glutathione S-transferase (GST), (GSH) activity Acetylcholinesterase (AChE) exposed groups, along increased nitric oxide production. overall observations show indices correlating upregulated expression apoptotic cells dose-dependent Collectively, our findings reveal an anxiogenic neuroactive compound capable inducing through mechanism involving oxidative-stress-induced larvae, which suggests potential hazard this can extrapolated health well.

Language: Английский

Citations

56
Hydroxyl containing benzo[b]thiophene analogs mitigates the acrylamide induced oxidative stress in the zebrafish larvae by stabilizing the glutathione redox cycle DOI

B. Haridevamuthu,

Tamilvelan Manjunathan,

Ajay Guru

et al.

Life Sciences, Journal Year: 2022, Volume and Issue: 298, P. 120507 - 120507

Published: March 29, 2022

Language: Английский

Citations

50
Reverse pharmacology of Nimbin-N2 attenuates alcoholic liver injury and promotes the hepatoprotective dual role of improving lipid metabolism and downregulating the levels of inflammatory cytokines in zebrafish larval model DOI
Gokul Sudhakaran, Pandurangan Prathap, Ajay Guru

et al.

Molecular and Cellular Biochemistry, Journal Year: 2022, Volume and Issue: 477(10), P. 2387 - 2401

Published: May 16, 2022

Language: Английский

Citations

43
Neuroprotective Effects of Phytochemicals against Aluminum Chloride-Induced Alzheimer’s Disease through ApoE4/LRP1, Wnt3/β-Catenin/GSK3β, and TLR4/NLRP3 Pathways with Physical and Mental Activities in a Rat Model DOI Creative Commons
Ahmed M. Hamdan,

Fatimah Hussain J. Alharthi,

Ahmed Hadi Alanazi

et al.

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(8), P. 1008 - 1008

Published: Aug. 17, 2022

Alzheimer's disease (AD) is a neurodegenerative disorder that associated with abnormal cognition. AD aided in its initiation and progression by hereditary environmental factors. Aluminum (Al) neurotoxic agent causes oxidative stress, which linked to progression. Additionally, Nrf2/HO-1, APOE4/LRP1, Wnt3/β-catenin, TLR4/NLRP3 are the main signaling pathways involved pathogenesis. Several phytochemicals promising options delaying evolution.This study aimed at studying neuroprotective effects of some as morin (MOR), thymol (TML), thymoquinone (TMQ) on physical mental activities (PhM) Al chloride (AlCl3)-induced rat model. Another objective was determine specificity using molecular docking.Eighty male Dawley rats were divided into eight groups. Each group received: saline (control group), AlCl3, (ALAD), PhM, either alone or combination MOR, TML, and/or TMQ for five weeks. Animals then subjected behavioral evaluation. Brain tissues used histopathological biochemical analyses extent neurodegeneration. The effect AlCl3-induced stress also investigated.AlCl3 caused decline spatial learning memory, well changes brains rats. Phytochemicals combined PhM restored antioxidant activities, increased HO-1 Nrf2 levels, blocked inflammasome activation, apoptosis, TLR4 expression, amyloide-β generation, tau hyperphophorylation. They brought ApoE4 LRP1 levels back normal regulated Wnt3/β-catenin/GSK3β pathway.The use strategy reducing modulating TLR4/NLRP3, Wnt3/β-catenin/GSK-3β pathways.

Language: Английский

Citations

43
Neuroprotective and antioxidant capability of RW20 peptide from histone acetyltransferases caused by oxidative stress-induced neurotoxicity in in vivo zebrafish larval model DOI Creative Commons

Nagaram Prabha,

Ajay Guru, Ramasamy Harikrishnan

et al.

Journal of King Saud University - Science, Journal Year: 2022, Volume and Issue: 34(3), P. 101861 - 101861

Published: Jan. 25, 2022

Oxidative stress causes disruption of cell macromolecules and resulting neurodegeneration when the antioxidant defense mechanism is inhibited. emerging as a key therapeutic target for nerve agent toxicity. Therefore, treatment methods are novel effective approach to attenuate oxidative neurodegeneration. This study evaluated neuroprotective ability short peptide, RW20 derived from histone acetyltransferases (HATs). Using bioinformatics tools, amino acid sequence RF13 peptide with activity was predicted HAT Channa striatus transcriptome. The synthesized at various concentrations (10–50 μM) analyzed in vitro vivo assay. effect H2O2 stressed zebrafish larvae. results showed by restoring enzymes including SOD, CAT, GPx, GSH, GST treated RW20. In addition, indicator lipid peroxidation gradually declined an increase concentration peptides. It also shown neuronal damage inhibiting nitric oxide overproduction, thus increasing AChE activity. Further, validate RW20, intracellular ROS level apoptosis larvae were incubating along which decreased reduced death. Overall, demonstrated that possessed prominent Hence, we conclude can be developed potent against stress-induced neurodegenerative diseases.

Language: Английский

Citations

40
Lipid-Lowering and Antioxidant Activity of RF13 Peptide From Vacuolar Protein Sorting-Associated Protein 26B (VPS26B) by Modulating Lipid Metabolism and Oxidative Stress in HFD Induced Obesity in Zebrafish Larvae DOI
Ajay Guru, Manikandan Velayutham, Jesu Arockiaraj

et al.

International Journal of Peptide Research and Therapeutics, Journal Year: 2022, Volume and Issue: 28(2)

Published: March 1, 2022

Language: Английский

Citations

39
Withaferin A targets the membrane of Pseudomonas aeruginosa and mitigates the inflammation in zebrafish larvae; an in vitro and in vivo approach DOI
Raghul Murugan, Ravi Rajesh, Seenivasan Boopathi

et al.

Microbial Pathogenesis, Journal Year: 2022, Volume and Issue: 172, P. 105778 - 105778

Published: Sept. 15, 2022

Language: Английский

Citations

39
Lemon-derived nanovesicles achieve antioxidant and anti-inflammatory effects activating the AhR/Nrf2 signaling pathway DOI Creative Commons
Ornella Urzì, Marco Cafora, Nima Rabienezhad Ganji

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(7), P. 107041 - 107041

Published: June 8, 2023

In the last years, extracellular vesicles (EVs) from different plant matrices have been isolated and gained interest of scientific community for their intriguing biological properties. this study, we characterized nanovesicles lemon juice (LNVs) evaluated antioxidant effects. We tested LNV activity using human dermal fibroblasts that were pre-treated with LNVs 24 h then stimulated hydrogen peroxide (H

Language: Английский

Citations

27
Antioxidants: an approach for restricting oxidative stress induced neurodegeneration in Alzheimer’s disease DOI
Sonal Dubey, Ekta Singh

Inflammopharmacology, Journal Year: 2023, Volume and Issue: 31(2), P. 717 - 730

Published: March 18, 2023

Language: Английский

Citations

25
Furan-based chalcone protects β-cell damage and improves glucose uptake in alloxan-induced zebrafish diabetic model via influencing Peroxisome Proliferator-Activated Receptor agonists (PPAR-γ) signaling DOI
S.P. Ramya Ranjan Nayak,

B. Haridevamuthu,

Raghul Murugan

et al.

Process Biochemistry, Journal Year: 2024, Volume and Issue: 142, P. 149 - 161

Published: April 10, 2024

Language: Английский

Citations

11