Zingiber officinale-derived exosome-like nanoparticles as antimicrobial agents via lipid-mediated inhibition DOI Creative Commons

C.-G. Zhu,

Bihu Gao,

Jiao Li

et al.

Nano Express, Journal Year: 2024, Volume and Issue: 5(4), P. 045009 - 045009

Published: Nov. 14, 2024

Abstract Microbial diseases represent a significant challenge to global health, highlighting the importance of developing novel alternative antibacterial agents. The widely used spice and traditional medicinal plant Zingiber officinale its derivatives have been demonstrated possess promising properties. aim present study was assess efficacy -derived exosome-like nanoparticles (ZODENs) against model bacterium, Escherichia coli ( E. ). ZODENs were isolated from rhizome subjected morphological, size distribution, zeta potential characterization. bacterial growth curve assays colony formation that remarkably inhibited following incubation with ZODENs. observed be internalized by bacteria incubation, subsequently remarkable killing effects. Furthermore, ZODEN-derived lipids, rather than remaining fractions, capacity inhibit cells, indicating activity at least partially attributable lipid content. These findings paved way for development ZODEN-based agents will facilitate future clinical translation plant-derived nanomedicines.

Language: Английский

Air pollution and skin diseases: A comprehensive evaluation of the associated mechanism DOI Creative Commons
Xiaoyu Gu, Zhengrui Li, Juan Su

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 278, P. 116429 - 116429

Published: May 7, 2024

Air pollutants deteriorate the survival environment and endanger human health around world. A large number of studies have confirmed that air pollution jeopardizes multiple organs, such as cardiovascular, respiratory, central nervous systems. Skin is largest organ first barrier protects us from outside particulate matter (PM), polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds (VOCs) will affect structure function skin bring about development inflammatory diseases (atopic dermatitis (AD), psoriasis), accessory (acne, alopecia), auto-immune (cutaneous lupus erythematosus(CLE) scleroderma), even tumors (melanoma, basal cell carcinoma (BCC), squamous-cell (SCC)). Oxidative stress, damage, microbiome dysbiosis, inflammation are pathogenesis stimulation. In this review, we summarize current evidence on effects possible mechanisms to provide strategies for future research.

Language: Английский

Citations

18
Multifaceted Therapeutic Potential of Plant-Derived Exosomes: Immunomodulation, Anticancer, Anti-Aging, Anti-Melanogenesis, Detoxification, and Drug Delivery DOI Creative Commons
Arzu Zeynep Karabay, Jaleh Barar, Yalda Hekmatshoar

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 394 - 394

Published: March 10, 2025

Most eukaryotic and prokaryotic cells have the potential to secrete a group of structures/membrane-bound organelles, collectively referred as extracellular vesicles (EVs), which offer several advantages producer/receiver cells. This review provides an overview EVs from plant sources with emphasis on their health-promoting possible use therapeutic agents. highlights essential biological effects plant-derived vesicles, including immune modulation, anticancer activities, protection against chemical toxicity pathogens, well anti-aging, anti-melanogenesis, anti-arthritic effects, along ongoing clinical studies. Evidence revealed that EVs’ contents exert beneficial properties through regulating important signaling pathways by transferring miRNAs other components. Taken all together, data proposed can be utilized nutritional compounds agents, such drug carriers. However, this emerging research area requires further in vitro/in vivo studies trials determine exact underlying mechanisms positive health treating various diseases.

Language: Английский

Citations

2
Emerging Drug Delivery Vectors: Engineering of Plant-Derived Nanovesicles and Their Applications in Biomedicine DOI Creative Commons
Luyao Yang,

Chao-Qing Li,

Yulin Zhang

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 2591 - 2610

Published: March 1, 2024

Extracellular vesicles can transmit intercellular information and transport biomolecules to recipient cells during various pathophysiological processes in the organism. Animal cell exosomes have been identified as potential nanodrugs delivery vehicles, yet they some shortcomings such high immunogenicity, cytotoxicity, complicated preparation procedures. In addition exosomes, plant-derived extracellular (PDVs), which carry a variety of active substances, are another promising nano-transport vehicles emerging recent years due their stable physicochemical properties, wide source, low cost. This work briefly introduces collection characterization PDVs, then focuses on application PDVs natural or engineered drug carriers biomedicine, finally discusses development challenges future applications.

Language: Английский

Citations

13
Lemon-Derived Exosome-Like Nanovesicles: A Promising Avenue for Drug Delivery System and Therapeutic Applications in Biomedical Science DOI

Mansi Khadilkar,

Sanchit Arora,

Rohan Chand Sahu

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106474 - 106474

Published: March 1, 2025

Language: Английский

Citations

1
Industrial-produced lemon nanovesicles ameliorate experimental colitis-associated damages in rats via the activation of anti-inflammatory and antioxidant responses and microbiota modification DOI Open Access
Vincenza Tinnirello, Maria Grazia Zizzo, Alice Conigliaro

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 174, P. 116514 - 116514

Published: April 3, 2024

Plant-derived nanovesicles (PDNVs) have recently emerged as natural delivery systems of biofunctional compounds toward mammalian cells. Considering their already described composition, anti-inflammatory properties, stability, and low toxicity, PDNVs offer a promising path for developing new preventive strategies several inflammatory diseases, among which the bowel disease (IBD). In this study, we explore protective effects industrially produced lemon vesicles (iLNVs) in rat model IBD. Characterization iLNVs reveals presence small particles less than 200 nm size profile bioactive enriched flavonoids organic acids with known beneficial properties. vitro studies on human macrophages confirm safety iLNVs, evidenced by reduced expression pro-inflammatory cytokines increased levels markers. As vivo experiments, pre-treatment significantly alleviates symptoms histological features 2,4 dinitrobenzensulfuric acid (DNBS)-induced colitis rats. Molecular pathway analysis modulation NF-κB Nrf2, indicating antioxidant effects. Finally, affects gut microbiota improving consistent colitis-related alterations. Overall, demonstrated role against emphasized potential managing IBD through multifaceted mechanisms.

Language: Английский

Citations

6
Advances in the study of plant-derived extracellular vesicles in the skeletal muscle system DOI Creative Commons

Xinning Mao,

Tenghui Li, Weihui Qi

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 204, P. 107202 - 107202

Published: May 3, 2024

Plant-derived extracellular vesicles (PDEV) constitute nanoscale entities comprising lipids, proteins, nucleic acids and various components enveloped by the lipid bilayers of plant cells. These play a crucial role in facilitating substance information transfer not only between cells but also across different species. Owing to its safety, stability, abundance raw materials, this has found extensive utilization recent years within research endeavors aimed at treating diseases. This article provides an overview pathways biological characteristics PDEV, along with prevalent methods employed for isolation, purification, storage. Furthermore, we comprehensively outline therapeutic implications diverse sources PDEV musculoskeletal system disorders. Additionally, explore as platforms engineering drug carriers, aiming delve deeper into significance potential contributions realm system.

Language: Английский

Citations

6
Molecular mechanisms and therapeutic application of extracellular vesicles from plants DOI
F Azizi, Salva Kazemipour Khabbazi, Stefania Raimondo

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: March 16, 2024

Language: Английский

Citations

5
Heat inactivation of foetal bovine serum performed after EV‐depletion influences the proteome of cell‐derived extracellular vesicles DOI Creative Commons
Ornella Urzì,

Markus Bergqvist,

Cecilia Lässer

et al.

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

The release of extracellular vesicles (EVs) in cell cultures as well their molecular cargo can be influenced by culture conditions such the presence foetal bovine serum (FBS). Although several studies have evaluated effect removing FBS-derived EVs ultracentrifugation (UC), less is known about influence FBS heat inactivation (HI) on cell-derived EVs. To assess this, three protocols based different combinations EV depletion UC and HI were evaluated, including ultracentrifuged but not inactivated (no-HI FBS), before (HI-before EV-depl after (HI-after FBS). We isolated large (L-EVs) small (S-EVs) from treated ways, we found that S-EV pellet HI-after was larger than no-HI HI-before FBS. Transmission electron microscopy, protein quantification, particle number evaluation showed significantly increased amount S-EVs had no significant L-EVs. Consequently, quantity lines cultured media supplemented with increased. Quantitative mass spectrometry analysis content when compared to Moreover, show quantified proteins could ascribed human origin, thus demonstrating mistakenly attributed conclude performed results changes proteome, molecules co-isolate contaminate used subsequent cultures. Our recommendation is, therefore, always perform prior depletion.

Language: Английский

Citations

4
Co-Treatment with Phlorotannin and Extracellular Vesicles from Ecklonia cava Inhibits UV-Induced Melanogenesis DOI Creative Commons
Kyung-A Byun, Young‐Jin Park, Seyeon Oh

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(4), P. 408 - 408

Published: March 28, 2024

Hyperpigmentation due to ultraviolet (UV)-induced melanogenesis causes various esthetic problems. Phlorotannin (PT) and extracellular vesicles (EVs) derived from plants suppress pathways. We used UV-exposed keratinocytes animal skin determine if co-treatment with PT EVs Ecklonia cava (EVE) could inhibit by reducing UV-induced oxidative stress the expression of thioredoxin-interacting protein (TXNIP)/nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3)/interleukin-18 (IL-18) pathway, which are upstream signals microphthalmia-associated transcription factor. UV exposure increased in skin, as evaluated 8-OHdG expression, this effect was reduced EVE. also binding between NLRP3 TXNIP, inflammasome activation IL-18 secretion, EVE skin. In melanocytes, conditioned media (CM) melanogenesis-related pathways; however, these effects were CM treated Similarly, treatment signals, melanin content, basement membrane (BM) components Thus, restored BM structure TXNIP/NLRP3/IL-18 pathway expression.

Language: Английский

Citations

4
Molecular anti-tumorigenic mechanism of Radix Polygoni Multiflori-derived exosome-like nanoparticles DOI Creative Commons

Mingqing Yang,

Lining Xu,

Weiyu Wang

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e41918 - e41918

Published: Jan. 22, 2025

The incidence and mortality rate of liver cancer are on the rise worldwide effective therapeutic strategies needed. Previous studies demonstrated that exosomes exosome-like nanoparticles (ELNs) have numerous biological activities including antioxidant, anti-tumor, anti-inflammatory effects. In this study, ELNs were successfully isolated for first time from Radix Polygoni Multiflori (RPM) juice by ultracentrifugation. This indicated RPM-ELNs could be internalized hepatoma cells effectively inhibit their proliferation migration in vitro. RNA sequencing qPCR experiments performed, results bioinformatic analyses revealed globally regulate expression levels genes associated with cell cycle. addition, it showed mainly target tissue mice vivo.

Language: Английский

Citations

0