3D Bioprinting of an Endothelialized Liver Lobule-like Construct as a Tumor-Scale Drug Screening Platform

Micromachines, Journal Year: 2023, Volume and Issue: 14(4), P. 878 - 878

Published: April 19, 2023

3D cell culture models replicating the complexity of cell–cell interactions and biomimetic extracellular matrix (ECM) are novel approaches for studying liver cancer, including in vitro drug screening or disease mechanism investigation. Although there have been advancements production cancer to serve as platforms, recreating structural architecture tumor-scale microenvironment native tumors remains a challenge. Here, using dot extrusion printing (DEP) technology reported our previous work, we fabricated an endothelialized lobule-like construct by hepatocyte-laden methacryloyl gelatin (GelMA) hydrogel microbeads HUVEC-laden microbeads. DEP enables be produced with precise positioning adjustable scale, facilitating construction structures. The vascular network was achieved sacrificing at 37 °C allow HUVEC proliferation on surface hepatocyte layer. Finally, used constructs anti-cancer (Sorafenib) screening, stronger resistance results were obtained when compared either mono-cultured spheroids alone. presented here successfully recreate morphology, may potential platform.

Language: Английский

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Current Biomedical Applications of 3D-Printed Hydrogels

Gels, Journal Year: 2023, Volume and Issue: 10(1), P. 8 - 8

Published: Dec. 21, 2023

Three-dimensional (3D) printing, also known as additive manufacturing, has revolutionized the production of physical 3D objects by transforming computer-aided design models into layered structures, eliminating need for traditional molding or machining techniques. In recent years, hydrogels have emerged an ideal printing feedstock material fabrication hydrated constructs that replicate extracellular matrix found in endogenous tissues. Hydrogels seen significant advancements since their first use contact lenses biomedical field. These led to development complex 3D-printed structures include a wide variety organic and inorganic materials, cells, bioactive substances. The most commonly used techniques fabricate hydrogel scaffolds are extrusion, jetting, vat photopolymerization, but novel methods can enhance resolution structural complexity printed emerged. applications be broadly classified four categories—tissue engineering regenerative medicine, cell culture disease modeling, drug screening toxicity testing, devices delivery systems. Despite applications, number challenges still addressed maximize printing. improving complexity, optimizing viability function, cost efficiency accessibility, addressing ethical regulatory concerns clinical translation.

Language: Английский

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Recent advances in biofabrication strategies based on bioprinting for vascularized tissue repair and regeneration

Materials & Design, Journal Year: 2023, Volume and Issue: 229, P. 111885 - 111885

Published: March 31, 2023

Vascularization plays a crucial role in transporting and exchanging nutrients oxygen between implanted grafts with the host tissue. In biofabrication of grafts, remodeling vascular networks can accelerate vascularized tissue repair regeneration. Given heterogeneity tissues, traditional scaffold manufacturing techniques cannot effectively achieve various scales vitro vivo biomimetic. recent years, 3D bioprinting technologies have been widely used fabricating for regeneration due to their shape customizability, simple procedure, reproducibility, precise multi-dimensional control. With rapid development technologies, bioprinting-based strategies gradually applied construction tissues. Based on this background, our study aimed review advances, challenges, future perspectives based The techniques, bioinks, seed cells, growth factors were also enrolled review. addition, history, vessel formation mechanism, histology discussed.

Language: Английский

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Integrated design and fabrication strategies based on bioprinting for skeletal muscle regeneration: Current status and future perspectives

Materials & Design, Journal Year: 2023, Volume and Issue: 225, P. 111591 - 111591

Published: Jan. 1, 2023

The number of skeletal muscle injuries derived from myopathies, exercise, and trauma, is growing due to increasing sports activities in normal life people. Skeletal has a robust capacity for regeneration following injury. However, few if any effective therapeutic options volumetric loss are available. Poor repair injury will lead dysfunction, resulting physical pain even seriously compromising daily life. As new technology biomedicine, 3D bioprinting widely used the field tissue engineering regenerative medicine its enormous advantages. creates biological structures similar that protists by precisely constructing specified geometric shape at micro/nano levels, thus addressing unmet needs replacement organ transplantation. With further application various repairs, more researchers utilized regeneration. Various bioinks, such as alginate, gelatin, fibrin hydrogels, extracellular matrix (ECM), loaded with functional seed cells or growth factors have been applied fabricating bioprinted constructs complicated inner Therefore, we conducted this review report process, development status technology, mechanism promoting repair. In addition, current challenges perspectives also discussed study.

Language: Английский

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Embedded bioprinted multicellular spheroids modeling pancreatic cancer bioarchitecture towards advanced drug therapy

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(7), P. 1788 - 1797

Published: Jan. 1, 2024

A facile embedded dot bioprinting system for bioengineering desmoplastic PDAC spheroids with scalable, flexible and robust performance, or multi-type spheroid patterns advanced drug therapy disease mechanism exploration, is introduced.

Language: Английский

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Automated detection and growth tracking of 3D bio-printed organoid clusters using optical coherence tomography with deep convolutional neural networks

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: April 12, 2023

Organoids are advancing the development of accurate prediction drug efficacy and toxicity in vitro. These advancements attributed to ability organoids recapitulate key structural functional features organs parent tumor. Specifically, self-organized assembly with a multi-scale structure 30-800 μm, which exacerbates difficulty non-destructive three-dimensional (3D) imaging, tracking classification analysis for organoid clusters by traditional microscopy techniques. Here, we devise 3D segmentation method based on Optical coherence tomography (OCT) technology deep convolutional neural networks (CNNs) printed (Organoid Printing optical tomography-based analysis, OPO). The results demonstrate that scale influences effect network. information-guided optimized EGO-Net designed achieves best results, especially showing better recognition workout biologically significant diameter ≥50 μm than other networks. Moreover, OPO reconstruct multiscale within microbeads calibrate printing errors segmenting edges. Overall, classification, quantitative image reveal growth process undergoes morphological changes such as volume growth, cavity creation fusion, calculation demonstrates rate is associated initial scale. new proposed enable study evolution heterogeneity cluster, valuable screening tumor sensitivity detection organoids.

Language: Английский

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Trends in Photopolymerizable Bioinks for 3D Bioprinting of Tumor Models

JACS Au, Journal Year: 2023, Volume and Issue: 3(8), P. 2086 - 2106

Published: Aug. 11, 2023

Three-dimensional (3D) bioprinting technologies involving photopolymerizable bioinks (PBs) have attracted enormous attention in recent times owing to their ability recreate complex structures with high resolution, mechanical stability, and favorable printing conditions that are suited for encapsulating cells. 3D bioprinted tissue constructs PBs can offer better insights into the tumor microenvironment platforms drug screening advance cancer research. These enable incorporation of physiologically relevant cell densities, tissue-mimetic stiffness, vascularized channels biochemical gradients models, unlike conventional two-dimensional (2D) cultures or other scaffold fabrication technologies. In this perspective, we present emerging techniques using context research, a specific focus on efforts recapitulate complexity microenvironment. We describe approaches various PB formulations compatible these along attempts bioprint models studying migration metastasis, cell–cell interactions, cell–extracellular matrix cancer. discuss limitations identify unexplored opportunities field clinical commercial translation

Language: Английский

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Pneumatic extrusion bioprinting-based high throughput fabrication of a melanoma 3D cell culture model for anti-cancer drug screening

Biomedical Materials, Journal Year: 2024, Volume and Issue: 19(5), P. 055034 - 055034

Published: Aug. 19, 2024

Abstract The high incidence of malignant melanoma highlights the need for in vitro models that accurately represent tumour microenvironment, enabling developments therapy and drug screening. Despite several advancements 3D cell culture models, appropriate evaluating efficacy are still demand. pneumatic extrusion-based bioprinting technology offers numerous benefits, including ability to achieve high-throughput capabilities. However, there is a lack research combines with analytical assays enable efficient screening models. To address this gap, study developed simple highly reproducible approach fabricate A375 model using technology. optimise method, parameters producing cultures 96-well plate were adjusted improve reproducibility while maintaining desired droplet size viability 92.13 ± 6.02%. cross-linking method was optimised by proliferation bioprinted cells three different concentrations calcium chloride. lower concentration 50 mM resulted higher increased after 9 d incubation. exhibited steadier rate cultures, tended aggregate into spheroids, whereas 2D generally formed monolayered sheets. In addition, we evaluated responses four anti-cancer drugs on both cultures. levels resistance all tested drugs. This presents cost-effective analysing do not add additional complexity current shows considerable potential advancing models’ evaluations.

Language: Английский

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High-throughput formulation of reproducible 3D cancer microenvironments for drug testing in myelogenous leukemia

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 12, 2024

Targeting cancer microenvironment is currently one of the major directions in drug development and preclinical studies leukemia. Despite variety available chronic myelogenous leukemia 3D culture models, reproducible generation miniaturized microenvironments, suitable for high-throughput testing, has remained a challenge. Here, we use microfluidics to generate over ten thousand highly monodisperse leukemic-bone marrow hydrogel microbeads per minute. We employ gelatin methacrylate (GelMA) as model extracellular matrix (ECM) tune concentration biopolymer, well other possible components ECM (fibrin, hyaluronic acid), cell ratio leukemic cells bone within microbeads. This allows achieve optimal viability propensity encapsulated microtissue formation, while also warranting long-term stability culture. administer kinase inhibitor, imatinib, at various concentrations and, via comparing mono- co-culture conditions (cancer alone vs cancer-stroma), find that stroma-leukemia crosstalk systematically protects against drug-induced cytotoxicity, confirming therefore our system mimics physiological stroma-dependent protection. finally discuss applicability (i) studying role direct- or close-contact interactions between embedded on stroma-mediated protection, (ii) screening anti-cancer therapeutics personalized therapies.

Language: Английский

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3D Nanofiber-Assisted Embedded Extrusion Bioprinting for Oriented Cardiac Tissue Fabrication

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 10(11), P. 7256 - 7265

Published: Oct. 19, 2024

Three-dimensional (3D) bioprinting technology stands out as a promising tissue manufacturing process to control the geometry precisely with cell-loaded bioinks. However, isotropic culture environment within bioink and lack of topographical cues impede formation oriented cardiac tissue. To overcome this limitation, we present novel method named 3D nanofiber-assisted embedded (3D-NFEP) fabricate an morphology. Aligned nanofiber scaffolds were fabricated by divergence electrospinning, which provided structural support for printing low-viscosity induction cardiomyocytes. Cells adhered aligned fibers after hydrogel degradation, high degree cell alignment was observed. This also demonstrated feasible solution multilayer printing. Therefore, 3D-NFEP is expected be applied structured engineering.

Language: Английский

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