Micromachines, Journal Year: 2024, Volume and Issue: 15(12), P. 1529 - 1529
Published: Dec. 23, 2024
Organ transplantation, biomimetic organ models, and the restoration of damaged or eviscerated tissues have been key goals in surgical medical research since their inception [...]
Language: Английский
Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 11
Published: Jan. 1, 2024
Abstract Biofabrication techniques allow for the construction of biocompatible and biofunctional structures composed from biomaterials, cells biomolecules. Bioprinting is an emerging 3D printing method which utilizes biomaterial-based mixtures with other biological constituents into printable suspensions known as bioinks. Coupled automated design protocols based on different modes droplet deposition, bioprinters are able to fabricate hydrogel-based objects specific architecture geometrical properties, providing necessary environment that promotes cell growth directs differentiation towards application-related lineages. For preparation such bioinks, various water-soluble biomaterials have been employed, including natural synthetic biopolymers, inorganic materials. Bioprinted constructs considered be one most promising avenues in regenerative medicine due their native organ biomimicry. a successful application, bioprinted should meet particular criteria optimal response, mechanical properties similar target tissue, high levels reproducibility fidelity, but also increased upscaling capability. In this review, we highlight recent advances bioprinting, focusing regeneration tissues bone, cartilage, cardiovascular, neural, skin organs liver, kidney, pancreas lungs. We discuss rapidly developing co-culture bioprinting systems used resemble complexity crosstalk between populations regeneration. Moreover, report basic physical principles governing ideal bioink biomaterials’ potential. examine critically present status regarding its applicability current limitations need overcome establish it at forefront artificial production transplantation.
Language: Английский
Citations
13
Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101515 - 101515
Published: Jan. 22, 2025
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1811 - 1811
Published: Feb. 2, 2024
Three-dimensional (3D) bioprinting is one of the most promising methodologies that are currently in development for replacement animal experiments. Bioprinting and alternative technologies rely on animal-derived materials, which compromises intent welfare results generation chimeric systems limited value. The current study therefore presents first bioprinted liver model entirely void constituents. Initially, HuH-7 cells underwent adaptation to a chemically defined medium (CDM). adapted exhibited high survival rates (85–92%) after cryopreservation freezing media, comparable those preserved standard (86–92%). Xeno-free bioink 3D yielded models with relative cell viability (97–101%), akin Matrigel-based (83–102%) 15 days culture. established xeno-free was used toxicity testing marine biotoxin, okadaic acid (OA). In 2D culture, OA virtually identical cultured under conditions CDM. model, 3-fold higher concentrations than respective monolayer culture were needed induce cytotoxicity. conclusion, this describes time its applicability research purposes.
Language: Английский
Citations
7
Gels, Journal Year: 2023, Volume and Issue: 10(1), P. 8 - 8
Published: Dec. 21, 2023
Three-dimensional (3D) printing, also known as additive manufacturing, has revolutionized the production of physical 3D objects by transforming computer-aided design models into layered structures, eliminating need for traditional molding or machining techniques. In recent years, hydrogels have emerged an ideal printing feedstock material fabrication hydrated constructs that replicate extracellular matrix found in endogenous tissues. Hydrogels seen significant advancements since their first use contact lenses biomedical field. These led to development complex 3D-printed structures include a wide variety organic and inorganic materials, cells, bioactive substances. The most commonly used techniques fabricate hydrogel scaffolds are extrusion, jetting, vat photopolymerization, but novel methods can enhance resolution structural complexity printed emerged. applications be broadly classified four categories—tissue engineering regenerative medicine, cell culture disease modeling, drug screening toxicity testing, devices delivery systems. Despite applications, number challenges still addressed maximize printing. improving complexity, optimizing viability function, cost efficiency accessibility, addressing ethical regulatory concerns clinical translation.
Language: Английский
Citations
16
Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(7), P. 1788 - 1797
Published: Jan. 1, 2024
A facile embedded dot bioprinting system for bioengineering desmoplastic PDAC spheroids with scalable, flexible and robust performance, or multi-type spheroid patterns advanced drug therapy disease mechanism exploration, is introduced.
Language: Английский
Citations
5
Tissue Engineering Part A, Journal Year: 2024, Volume and Issue: 30(13-14), P. 387 - 408
Published: Jan. 11, 2024
Bioprinting describes the printing of biomaterials and cell-laden or cell-free hydrogels with various combinations embedded bioactive molecules. It encompasses precise patterning cells to create scaffolds for different biomedical needs. There are many requirements that bioprinting face, it is ultimately interplay between scaffold's structure, properties, processing, performance will lead its successful translation. Among essential properties must possess—adequate appropriate application-specific chemical, mechanical, biological performance—the mechanical behavior hydrogel-based bioprinted key their stable in vivo at site implantation. Hydrogels typically constitute main scaffold material medium biomolecules very soft, often lack sufficient stability, which reduces printability and, therefore, potential. The aim this review article highlight reinforcement strategies used approaches achieve enhanced stability bioinks printed scaffolds. Enabling robust materials processes creation truly complex remarkable structures could accelerate application smart, functional settings. a powerful tool fabrication 3D applications. has gained tremendous attention recent years, bioink library expanding include more combinations. From practical perspective, need be considered, such as structure's performances. these, constructs critical translation into clinic. explore stabilization structures.
Language: Английский
Citations
4
MedComm, Journal Year: 2024, Volume and Issue: 5(10)
Published: Sept. 23, 2024
Bioprinting is a highly promising application area of additive manufacturing technology that has been widely used in various fields, including tissue engineering, drug screening, organ regeneration, and biosensing. Its primary goal to produce biomedical products such as artificial implant scaffolds, tissues organs, medical assistive devices through software-layered discrete numerical control molding. Despite its immense potential, bioprinting still faces several challenges. It requires concerted efforts from researchers, engineers, regulatory bodies, industry stakeholders are principal overcome these challenges unlock the full potential bioprinting. This review systematically discusses principles, applications, future perspectives while also providing topical overview research progress over past two decades. The most recent advancements comprehensively reviewed here. First, printing techniques methods summarized along with related bioinks supporting structures. Second, interesting representative cases regarding applications biosensing introduced detail. Finally, remaining suggestions for directions proposed discussed. one areas fields. aims devices. perspectives, which provides description
Language: Английский
Citations
4
Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 34
Published: Jan. 1, 2025
Language: Английский
Citations
0
Micromachines, Journal Year: 2025, Volume and Issue: 16(5), P. 505 - 505
Published: April 26, 2025
Over the past two decades, additive manufacturing has advanced significantly, enabling rapid fabrication of functional components across various applications. In medical devices, it been used for prototyping, prosthetics, drug delivery platforms, and more recently, tissue scaffolding. However, current technologies face challenges, particularly in depositing conformal layers over curved surfaces. This study introduces a novel multi-nozzle extrusion printer concept designed to deposit soft gel onto A custom clearance locking mechanism enhances printer’s ability achieve coatings on both flat substrates. We investigate key deposition parameters, including displacement volume nozzle configuration, while comparing sequences: “Press Express” “Express Press”. Our results demonstrate that Press” technique yields uniform, merged than method. technology holds promise further refinement potential applications engineering.
Language: Английский
Citations
0
Journal of Applied Physics, Journal Year: 2024, Volume and Issue: 135(14)
Published: April 8, 2024
3D printing techniques allow for the precise placement of living cells, biological substances, and biochemical components, establishing themselves as a promising approach in bioengineering. Recently, has been applied to develop human-relevant vitro cancer models with highly controlled complexity potential method drug screening disease modeling. Compared 2D culture, 3D-printed more closely replicate vivo microenvironment. Additionally, they offer reduction ethical issues associated using animal models. This focused review discusses relevance technologies cells materials used cutting-edge microfluidic device systems. Future prospective solutions were discussed establish reliable tools understanding mechanisms.
Language: Английский
Citations
3