A Library of ROS‐Catalytic Metalloenzyme Mimics with Atomic Metal Centers DOI
Sujiao Cao,

Zhenyang Zhao,

Yijuan Zheng

et al.

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(16)

Published: Feb. 8, 2022

MetalN-coordinated centers supported by carbonaceous substrates have emerged as promising artificial metalloenzymes (AMEs) to mimic the biocatalytic effects of their natural counterparts. However, synthesis well-defined AMEs that contain different atomic metalN but present similar physicochemical and coordination structures remains a substantial challenge. Here, 20 types with geometries metalN-coordinated are synthesized compare disclose catalytic activities, substrate selectivities, kinetics, reactive oxygen species (ROS) products. Their oxidase (OXD)-, peroxidase (POD)-, halogen (HPO)-mimetic behaviors systematically explored. The Fe-AME shows highest OXD- HPO-mimetic activities compared other due its high vmax (0.927 × 10-6 m s-1 ) low Km (1.070 10-3 m), while Cu-AME displays best POD-like performance. Furthermore, theoretical calculation reveals ROS-catalytic paths highly related electronic metal centers. Benefiting from facile adsorption H2 O2 molecule lower energy barrier generating •O2- , higher performances than Mn-AME. engineered show not only remarkably also provide new guidance toward developing biocatalysts for broad application fields.

Language: Английский

Carbon-based nanozymes: Design, catalytic mechanism, and bioapplication DOI
Yun Sun, Bolong Xu, Xueting Pan

et al.

Coordination Chemistry Reviews, Journal Year: 2022, Volume and Issue: 475, P. 214896 - 214896

Published: Oct. 27, 2022

Language: Английский

Citations

143
Direct and Specific Detection of Glyphosate Using a Phosphatase-like Nanozyme-Mediated Chemiluminescence Strategy DOI

Jiafu Chang,

Yu Lei, Ting Hou

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(9), P. 4479 - 4485

Published: Feb. 21, 2023

Most organophosphorus pesticide (OP) sensors reported in the literature rely on inhibition effect of OPs activity acetylcholinesterase (AChE), which suffer from drawbacks lack selective recognition OPs, high cost, and poor stability. Herein, we proposed a novel chemiluminescence (CL) strategy for direct detection glyphosate (an herbicide) with sensitivity specificity, is based porous hydroxy zirconium oxide nanozyme (ZrOX-OH) obtained via facile alkali solution treatment UIO-66. ZrOX-OH displayed excellent phosphatase-like activity, could catalyze dephosphorylation 3-(2′-spiroadamantyl)-4-methoxy-4-(3′-phosphoryloxyphenyl)-1,2-dioxetane (AMPPD) to generate strong CL. The experimental results showed that closely related content hydroxyl groups their surface. Interestingly, properties exhibited unique response because consumption surface group by carboxyl glyphosates was thus employed develop CL sensor without using bio-enzymes. recovery cabbage juice ranged 96.8 103.0%. We believe as-proposed supplies simpler more highly approach OP assay provides new method development analysis real samples.

Language: Английский

Citations

143
A Glucose‐Powered Activatable Nanozyme Breaking pH and H2O2 Limitations for Treating Diabetic Infections DOI

Lifang Chen,

Shuohui Xing,

Yanli Lei

et al.

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(44), P. 23534 - 23539

Published: Aug. 11, 2021

Abstract The peroxidase‐like activity of nanozymes is promising for chemodynamic therapy by catalyzing H 2 O into . OH. However, most nanozymes, this optimal just in acidic solutions, while the pH physiological systems beyond 7.0 (even >8.0 chronic wounds) with inadequate We herein communicate an activatable nanozyme targeting capability to simultaneously break local and limitations under conditions. As a proof concept, aptamer‐functionalized glucose oxidase, hyaluronic acid constitute nanocapsule “APGH”, which can be activated bacteria‐secreted hyaluronidase infected wounds. Nanozymes bind onto bacteria through aptamer recognition, oxidation tunes down supplements in‐situ generation OH on surfaces. switching enhanced antibacterial effect were verified vitro diabetic This strategy directly regulating microenvironment generally accessible significant facilitating biological applications nanozymes.

Language: Английский

Citations

137
Bimetallic oxide Cu1.5Mn1.5O4 cage-like frame nanospheres with triple enzyme-like activities for bacterial-infected wound therapy DOI
Konglin Wu, Dongdong Zhu, Xingliang Dai

et al.

Nano Today, Journal Year: 2022, Volume and Issue: 43, P. 101380 - 101380

Published: Jan. 22, 2022

Language: Английский

Citations

117
A Library of ROS‐Catalytic Metalloenzyme Mimics with Atomic Metal Centers DOI
Sujiao Cao,

Zhenyang Zhao,

Yijuan Zheng

et al.

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(16)

Published: Feb. 8, 2022

MetalN-coordinated centers supported by carbonaceous substrates have emerged as promising artificial metalloenzymes (AMEs) to mimic the biocatalytic effects of their natural counterparts. However, synthesis well-defined AMEs that contain different atomic metalN but present similar physicochemical and coordination structures remains a substantial challenge. Here, 20 types with geometries metalN-coordinated are synthesized compare disclose catalytic activities, substrate selectivities, kinetics, reactive oxygen species (ROS) products. Their oxidase (OXD)-, peroxidase (POD)-, halogen (HPO)-mimetic behaviors systematically explored. The Fe-AME shows highest OXD- HPO-mimetic activities compared other due its high vmax (0.927 × 10-6 m s-1 ) low Km (1.070 10-3 m), while Cu-AME displays best POD-like performance. Furthermore, theoretical calculation reveals ROS-catalytic paths highly related electronic metal centers. Benefiting from facile adsorption H2 O2 molecule lower energy barrier generating •O2- , higher performances than Mn-AME. engineered show not only remarkably also provide new guidance toward developing biocatalysts for broad application fields.

Language: Английский

Citations

116