Antibiotic-Resistant Bacteria in Environmental Water Sources from Southern Chile: A Potential Threat to Human Health DOI Creative Commons

Matías Jofré Bartholin,

Boris Barrera,

Liliana Berrocal Silva

et al.

Microbiology Research, Journal Year: 2023, Volume and Issue: 14(4), P. 1764 - 1773

Published: Nov. 1, 2023

Antimicrobial resistance (AMR) is a critical global issue affecting public and animal health. The overuse of antibiotics in human health, production, agriculture, aquaculture has led to the selection antibiotic-resistant strains, particularly Gram-negative bacteria. Mutations horizontal gene transfer play significant role development antimicrobial resistance, leading reduced efficacy current antibiotics. Today, AMR bacteria antibiotic-resistance genes (ARGs) are increasingly recognized multiple environmental sources, including recreational irrigation waters. This study aims identify from surface aquatic reservoirs southern Chile assess their susceptibility clinically relevant Water samples were collected four lakes, five rivers, one waterfall, watershed isolate bacilli (GNB). API-20E MALDI–TOF employed for bacterial identification. Kirby–Bauer disc diffusion tests multiplex PCR performed determine profile. A total 26 GNB strains isolated water samples, predominantly belonging Pseudomonas (n = 9) Acinetobacter 7) genera. Among these 96.2% resistant ampicillin cefazoline, while 26.9% 34.6% showed ceftazidime cefepime, respectively. Additionally, 38.5% exhibited colistin. Two Enterobacter cloacae obtained Cachapoal River (sixth region) Villarrica Lake (ninth region), respectively, presented multidrug-resistant (MDR) phenotype carried at least two extended-spectrum β-lactamase (ESBL) genes. Thus, ARGs found natural reservoirs, raising concerns about dissemination determinants among potentially pathogenic microbial communities.

Language: Английский

Nanosilver: An Old Antibacterial Agent with Great Promise in the Fight against Antibiotic Resistance DOI Creative Commons

Kyra G. Kaiser,

Victoire Delattre,

Victoria Frost

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(8), P. 1264 - 1264

Published: July 31, 2023

Antibiotic resistance in bacteria is a major problem worldwide that costs 55 billion USD annually for extended hospitalization, resource utilization, and additional treatment expenditures the United States. This review examines roles forms of silver (e.g., bulk Ag, salts (AgNO

Language: Английский

Citations

37
Current Uses and Future Perspectives of Genomic Technologies in Clinical Microbiology DOI Creative Commons
Irene Bianconi,

Richard Aschbacher,

Elisabetta Pagani

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(11), P. 1580 - 1580

Published: Oct. 30, 2023

Recent advancements in sequencing technology and data analytics have led to a transformative era pathogen detection typing. These developments not only expedite the process, but also render it more cost-effective. Genomic analyses of infectious diseases are swiftly becoming standard for analysis control. Additionally, national surveillance systems can derive substantial benefits from genomic data, as they offer profound insights into epidemiology emergence antimicrobial-resistant strains. Antimicrobial resistance (AMR) is pressing global public health issue. While clinical laboratories traditionally relied on culture-based antimicrobial susceptibility testing, integration AMR holds immense promise. Genomic-based furnish swift, consistent, highly accurate predictions phenotypes specific strains or populations, all while contributing invaluable surveillance. Moreover, genome assumes pivotal role investigation hospital outbreaks. It aids identification infection sources, unveils genetic connections among isolates, informs strategies The One Health initiative, with its focus intricate interconnectedness humans, animals, environment, seeks develop comprehensive approaches disease surveillance, control, prevention. When integrated epidemiological systems, forecast expansion bacterial populations species transmissions. Consequently, this provides evolution relationships pathogens, hosts, environment.

Language: Английский

Citations

28
Population structure and antibiotic resistance of swine extraintestinal pathogenic Escherichia coli from China DOI Creative Commons
Xudong Li, Huifeng Hu, Yongwei Zhu

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 10, 2024

Abstract Extraintestinal Pathogenic Escherichia coli (ExPEC) pose a significant threat to human and animal health. However, the diversity antibiotic resistance of ExPEC, their connection infections, remain largely unexplored. The study performs large-scale genome sequencing testing 499 swine-derived ExPEC isolates from China. Results show swine are phylogenetically diverse, with over 80% belonging phylogroups B1 A. Importantly, 15 exhibit genetic relatedness human-origin E. strains. Additionally, 49 strains harbor toxins typical enteric pathotypes, implying hybrid pathotypes. Notably, 97% total multidrug resistant, including critical drugs like third- fourth-generation cephalosporins. Correspondingly, genomic analysis unveils prevalent genes (ARGs), often associated co-transfer mechanisms. Furthermore, 20 complete genomes illuminates transmission pathways ARGs within pathogens. For example, plasmids co-harboring fosA3 , bla CTX-M-14 mcr-1 between Salmonella enterica is observed. These findings underscore importance monitoring controlling infections in animals, as they can serve reservoir potential affect health or even be origin pathogens infecting humans.

Language: Английский

Citations

11
Preventing Antimicrobial Resistance Together: Reflections on AMR Week 2023 DOI Creative Commons
Jaffar A. Al‐Tawfiq, Shahul H. Ebrahim, Ziad A. Memish

et al.

Journal of Epidemiology and Global Health, Journal Year: 2024, Volume and Issue: 14(2), P. 249 - 251

Published: Jan. 8, 2024

System CDCCenters for Disease Control and Prevention in the USA Human life expectancy improved worldwide during last century largely attributable to foundational changes health enabling factors such as clean drinking water, sanitation, discovery of antibiotics.Of these three developments, antibiotics are both a blessing challenge continued human prosperity.The overuse misuse antibiotics, antivirals, antifungals have led emergence resistant strains bacteria, viruses, fungi.Antimicrobial resistance (AMR) is not distant, abstract menace; it clear present danger slow motion, with implications animal health.

Language: Английский

Citations

10
Antibiotic Resistance Genes and Microbiota in Brassica oleracea var. acephala Cultivated in South Korea: Potential for Resistance Transmission DOI Creative Commons

Su Jin Yum,

Seon Yeong Yu,

Seung Min Kim

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Antimicrobial resistance (AMR) poses a critical global public health challenge. This study investigates the microbiome of Brassica oleracea var. acephala (kale) to evaluate role food production systems, particularly plant-derived foods, in AMR dissemination. Using 16S rRNA gene sequencing and metagenomic shotgun sequencing, we analyzed microbial diversity antimicrobial genes (ARGs) kale samples. Results showed significant regional differences microbiota composition ARG distribution, with traditional fertilizer use linked higher prevalence coliform bacteria compared farms using other fertilization methods. Additionally, confirmed transfer potential by Klebsiella pneumoniae within populations. Storage conditions notably affected dynamics, temperatures promoting K. growth washed These findings revealed importance research foods highlight need for improved agricultural practices mitigate risks associated high abundance bacteria.

Language: Английский

Citations

1
Metagenomic assembly is the main bottleneck in the identification of mobile genetic elements DOI Creative Commons
Jesse Kerkvliet, Alex Bossers, Jannigje G. Kers

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e16695 - e16695

Published: Jan. 4, 2024

Antimicrobial resistance genes (ARG) are commonly found on acquired mobile genetic elements (MGEs) such as plasmids or transposons. Understanding the spread of associated with (mARGs) across different hosts and environments requires linking ARGs to existing reservoir within bacterial communities. However, reconstructing mARGs in metagenomic data from diverse ecosystems poses computational challenges, including genome fragment reconstruction (assembly), high-throughput annotation MGEs, identification their association ARGs. Recently, several bioinformatics tools have been developed identify assembled fragments plasmids, phages, insertion sequence (IS) data. These methods can help understanding dissemination mARGs. To streamline process identifying multiple samples, we combined these an automated open-source pipeline, MetaMobilePicker, that identifies IS starting short sequencing reads. This pipeline was used three a simplified simulated metagenome dataset, comprising whole sequences seven clinically relevant species containing 55 ARGs, nine five phages. The results demonstrated moderate precision for (0.57) phages (0.71), sensitivity (0.58) (0.70). In this study, aim assess main causes performance MGE prediction comprehensive manner. We conducted systematic benchmark, considering read coverage, contig length cutoffs investigating classification algorithms. Our analysis revealed assembly is primary bottleneck when identified MGEs short-read metagenomics experiments rather than by tools.

Language: Английский

Citations

7
Intersections between climate change and antimicrobial resistance: a systematic scoping review DOI Creative Commons
Bianca van Bavel, James D. Ford,

Kwang-Kyun Moon

et al.

The Lancet Planetary Health, Journal Year: 2024, Volume and Issue: 8(12), P. e1118 - e1128

Published: Dec. 1, 2024

Climate change and antimicrobial resistance (AMR) present crucial challenges for the health wellbeing of people, animals, plants, ecosystems worldwide, yet two are largely treated as separate unrelated challenges. The aim this systematic scoping Review is to understand nature growing evidence base linking AMR climate identify knowledge gaps areas further research. We conducted a search peer-reviewed literature in Scopus, Web Science, PubMed on 27 June, 2022. Our strategy identified screened 1687 unique results. Data were extracted analysed from 574 records meeting our inclusion criteria. 222 (39%) these reviewed articles discussed harmful synergies which both exist independently can interact synergistically, resulting negative outcomes. Just over quarter (n=163; 28%) contained general or broad references change, whereas fifth (n=111; 19%) referred influencing emergence evolution AMR. 12% (n=70) presented positive between approaches aimed at addressing interventions targeting management control remaining focused shared drivers trade-offs actions that have unanticipated outcomes (or vice versa), and, finally, pathways through negatively influence change. findings indicate multiple intersections do connect. Research area still nascent, disciplinarily isolated, only beginning converge, with few documents primarily equal intersection topics. Greater empirical evidence-based attention needed investigate related specific hazards resistant fungi, helminths, protists, viruses.

Language: Английский

Citations

6
Multidrug-Resistant Commensal and Infection-Causing Staphylococcus spp. Isolated from Companion Animals in the Valencia Region DOI Creative Commons
Ana Marco-Fuertes, Clara Marín,

Concepción Gimeno-Cardona

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(2), P. 54 - 54

Published: Jan. 26, 2024

The emergence of antimicrobial resistance (AMR) and multidrug (MDR) among microorganisms to commonly used antibiotics is a growing concern in both human veterinary medicine. Companion animals play significant role the epidemiology AMR, as their population continuously increasing, posing risk disseminating particularly strains public health importance, such methicillin-resistant Staphylococcus strains. Thus, this study aimed investigate prevalence AMR MDR commensal infection-causing spp. dogs cats Valencia region. For purpose, 271 samples were taken from centers assess susceptibility against 20 antibiotics, including some most important for treatment infections, five last resort list. Of all samples, 187 recovered asymptomatic skin-diseased cats, which S. pseudintermedius (≈60%) was more prevalent dogs, while felis (≈50%) cats. In overall analysis isolates, observed tested, those crucial Furthermore, over 70% 30% respectively, exhibited MDR. This highlights significance monitoring trends companion animals. potential contribution these dissemination its genes humans, other animals, shared environment underscores necessity adopting One Health approach.

Language: Английский

Citations

5
Soil microbial ecology through the lens of metatranscriptomics DOI Open Access
Jingjing Peng, Xi Zhou, Christopher Rensing

et al.

Soil Ecology Letters, Journal Year: 2023, Volume and Issue: 6(3)

Published: Dec. 28, 2023

Language: Английский

Citations

12
Global dissemination of the beta-lactam resistance gene blaTEM-1 among pathogenic bacteria DOI
Adeel Farooq,

Asma Rafique,

Eunyoung Han

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 963, P. 178521 - 178521

Published: Jan. 17, 2025

Language: Английский

Citations

0