The impact of antibiotic induction on virulence and antibiotic resistance in Klebsiella pneumoniae: a comparative study of CSKP and CRKP strains DOI Creative Commons
Keda Chen,

Wei Chen,

Qian Zhang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 21, 2024

Background Klebsiella pneumoniae is an opportunistic pathogen causing nosocomial infections, classified into carbapenem-sensitive and carbapenem-resistant strains. Understanding the virulence factors antibiotic resistance of these strains essential for effective clinical management. Objective This study compared genes profiles 50 CSKP CRKP strains, examining their expression under pressure mechanisms contributing to pathogenicity. Methods Virulence ( rmpA , rmpA2 iucA iutA Peg-344 ybts iroB ) were detected in both using polymerase chain reaction (PCR). Antibiotic susceptibility testing established minimum inhibitory concentrations (MICs) key antibiotics. Gene analysis was performed with quantitative reverse transcription PCR (qRT-PCR) after 10 days exposure. Results exhibited significantly higher positivity rates predominantly expressed KPC SHV CTX-M3 whereas no found CSKP. tests showed increased MICs, particularly ciprofloxacin imipenem, following induction. demonstrated elevated while Conclusion highlights intricate relationship between . show strong factor expression, adapt through altered gene patterns. These findings underscore urgent need continuous surveillance innovative therapeutic strategies combat multidrug-resistant infections.

Language: Английский

Recent design strategies for boosting chemodynamic therapy of bacterial infections DOI Creative Commons
Junjie Zhang, Haiyang Guo,

Ming Liu

et al.

Exploration, Journal Year: 2023, Volume and Issue: 4(2)

Published: Oct. 17, 2023

Abstract The emergence of drug‐resistant bacteria poses a significant threat to people's lives and health as bacterial infections continue persist. Currently, antibiotic therapy remains the primary approach for tackling infections. However, escalating rates drug resistance coupled with lag in development novel drugs have led diminishing effectiveness conventional treatments. Therefore, nonantibiotic‐dependent therapeutic strategies has become imperative impede rise resistance. chemodynamic (CDT) opened up new possibility due CDT can convert H 2 O into •OH via Fenton/Fenton‐like reaction treatment. efficacy is limited by variety practical factors. To overcome this limitation, sterilization efficiency be enhanced introducing therapeutics inherent antimicrobial capability. In addition, researchers explored CDT‐based combined therapies augment its effects mitigate potential toxic side toward normal tissues. This review examines research progress field, explores various enhance presents synergistic combination other modalities. And last, current challenges faced future directions are discussed.

Language: Английский

Citations

55
Antimicrobial Hydrogels: Potential Materials for Medical Application DOI Open Access
Yanni Li,

Yujia Han,

Hongxia Li

et al.

Small, Journal Year: 2023, Volume and Issue: 20(5)

Published: Sept. 26, 2023

Abstract Microbial infections based on drug‐resistant pathogenic organisms following surgery or trauma and uncontrolled bleeding are the main causes of increased mortality from worldwide. The prevalence pathogens has led to a significant increase in medical costs poses great threat normal life people. This is an important issue field biomedicine, emergence new antimicrobial materials hydrogels holds promise for solving this problem. Hydrogel material with good biocompatibility, water absorption, oxygen permeability, adhesion, degradation, self‐healing, corrosion resistance, controlled release drugs as well structural diversity. Bacteria‐disturbing have applications direction surgical treatment, wound dressing, device coating, tissue engineering. paper reviews classification hydrogels, current status research, potential one application analyzes research biomedical five aspects: metal‐loaded drug‐loaded carbon‐material‐loaded fixed activity biological provides outlook high activity, biodegradability, injectability, clinical applicability future development prospects field.

Language: Английский

Citations

49
Fabrication of multifunctional hydrogels based on tannic acid-coated nanocrystalline cellulose DOI

Wancheng Song,

Jialin Guan,

Wei Wei

et al.

3 Biotech, Journal Year: 2025, Volume and Issue: 15(3)

Published: Feb. 22, 2025

Language: Английский

Citations

2
Polyrotaxanated covalent organic frameworks based on β-cyclodextrin towards high-efficiency synergistic inactivation of bacterial pathogens DOI
Qi Yue, Jie Yu,

Qipeng Zhu

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 486, P. 150345 - 150345

Published: March 11, 2024

Language: Английский

Citations

10
The Convergence of Antibiotic Contamination, Resistance, and Climate Dynamics in Freshwater Ecosystems DOI Open Access
Marcelo Pedrosa Gomes

Water, Journal Year: 2024, Volume and Issue: 16(18), P. 2606 - 2606

Published: Sept. 14, 2024

The convergence of antibiotic contamination, antimicrobial resistance (AMR), and climate dynamics poses a critical environmental public health challenge. Freshwater ecosystems are increasingly threatened by the persistent presence antibiotics, which, coupled with rising global temperatures, accelerate development spread AMR. This review examines sources, pathways, mechanisms through which antibiotics enter freshwater systems how change exacerbates these processes. discusses this convergence’s ecological human impacts, highlighting implications for biodiversity health. It also explored current monitoring mitigation strategies, including advanced oxidation processes, natural-based solutions, policy interventions. Finally, identifies research gaps proposes future directions managing intertwined threats resistance, change. emphasizes need integrated, multidisciplinary approaches to protect resources in an volatile environment.

Language: Английский

Citations

9
Understanding the bovine mastitis co-infections: Coexistence with Enterobacter alters S. aureus antibiotic susceptibility and virulence phenotype DOI

Paula Isaac,

María Laura Breser,

María Florencia De Lillo

et al.

Research in Veterinary Science, Journal Year: 2025, Volume and Issue: 185, P. 105547 - 105547

Published: Jan. 21, 2025

Language: Английский

Citations

1
Pooled Antibiotic Susceptibility Testing for Polymicrobial UTI Performs Within CLSI Validation Standards DOI Creative Commons

Richard A. Festa,

F. R. Cockerill,

Rick L. Pesano

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(2), P. 143 - 143

Published: Feb. 1, 2025

Background/Objectives: Urinary tract infections (UTIs) pose an increasing risk of antimicrobial resistance, and novel diagnostic tests have been developed to address the limitations standard urine culture in these cases. It is important that be validated for agreement error rates against antibiotic susceptibility testing (AST) methods. Methods: Polymicrobial (≥two non-fastidious microorganisms) consecutive clinical specimens submitted UTI were included this analysis. Specimens tested with Pooled Antibiotic Susceptibility Testing (P-AST) broth microdilution/disk diffusion (BMD/DD) parallel. Performance characteristics, such as essential (EA%), very major errors (VMEs), (MEs), assessed using Clinical Laboratory Standards Institute (CLSI) standards. P-AST-resistant BMD/DD consensus-sensitive results heteroresistance. Real-world sample data used assess associations between organism counts average “sensitive” count per sample. Results: The P-AST isolate AST was ≥90%, VMEs <2.0%, MEs <3.0%, meeting CLSI guidelines verification validation studies. When heteroresistance accounted for, overall both <1.5%. presence additional organisms dropped number antibiotics from 9.8 one 2.5 five or more organisms. fastidious did not any meaningful impact. Conclusions: P-AST, a component Guidance® assay (Pathnostics, Irvine, CA, USA), performed within standards polymicrobial specimens.

Language: Английский

Citations

1
Higher-order interactions and emergent properties of microbial communities: The power of synthetic ecology DOI Creative Commons

Óscar Adrián Gallardo-Navarro,

Bernardo Aguilar-Salinas,

Jorge Gustavo Rocha

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(14), P. e33896 - e33896

Published: July 1, 2024

Humans have long relied on microbial communities to create products, produce energy, and treat waste. The microbiota residing within our bodies directly impacts health, while the soil rhizosphere microbiomes influence productivity of crops. However, complexity diversity make them challenging study difficult develop into applications, as they often exhibit emergence unpredictable higher-order phenomena. Synthetic ecology aims at simplifying by constituting synthetic or semi-natural with reduced that become easier analyze. This strategy combines methodologies simplify existing complex systems (top-down approach) build system from its constituent components (bottom-up approach). Simplified are studied understand how interactions among populations shape behavior community model predict their response external stimuli. By harnessing potential through a multidisciplinary approach, we can advance knowledge ecological concepts address critical public agricultural, environmental issues more effectively.

Language: Английский

Citations

6
Supramolecular co-assembled hybrid hydrogels for antibacterial therapy DOI Creative Commons
Zakia Riaz,

Sravan Baddi,

Chuanliang Feng

et al.

Supramolecular Materials, Journal Year: 2024, Volume and Issue: 3, P. 100064 - 100064

Published: Jan. 18, 2024

Bacterial infections arising from antibiotic-resistant strains represent a formidable global health threat. In the realm of antibacterial therapies, supramolecular hydrogels stand out with unique advantages owing to their adaptable and flexible non-covalent interactions diverse biomolecules. Their ability encapsulate variety biologically active agents through co-assembly further amplifies efficacy, endowing these hybrid spectrum functionalities within unified system. Various encompass self-healing, injectability, tissue adhesion, facile drug loading, controlled release mechanisms, biocompatibility, properties, antioxidative attributes. This review article delves into two categories hydrogels: i) those intrinsic attributes ii) co-assembled formed integration an assortment agents, including various nanomaterials, antibiotics, compounds derived natural sources, polymers. The anticipated role hydrogels, coupled contemporary medical technologies devices, is paramount in development highly effective secure platforms for therapy. Furthermore, addresses current challenges while elucidating prospective future advancements this field.

Language: Английский

Citations

5
Gallium-based metal–organic frameworks loaded with antimicrobial peptides for synergistic killing of drug-resistant bacteria DOI
Shuo Liu,

Yuxin Ji,

Hangqi Zhu

et al.

Journal of Materials Chemistry B, Journal Year: 2023, Volume and Issue: 11(43), P. 10446 - 10454

Published: Jan. 1, 2023

One-step synthesis of gallium-based metal–organic frameworks loaded with antimicrobial peptides at room temperature for synergistic killing drug-resistant bacteria.

Language: Английский

Citations

12