Type I toxin-antitoxin systems in bacteria: from regulation to biological functions
EcoSal Plus,
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 20, 2024
ABSTRACT
Toxin-antitoxin
systems
are
ubiquitous
in
the
prokaryotic
world
and
widely
distributed
among
chromosomes
mobile
genetic
elements.
Several
different
toxin-antitoxin
system
types
exist,
but
what
they
all
have
common
is
that
toxin
activity
prevented
by
cognate
antitoxin.
In
type
I
systems,
production
controlled
an
RNA
antitoxin
structural
features
inherent
to
messenger
RNA.
Most
toxins
small
membrane
proteins
display
a
variety
of
cellular
effects.
While
originally
discovered
as
modules
stabilize
plasmids,
chromosomal
may
also
prophages,
or
serve
important
functions
upon
certain
stress
conditions
contribute
population-wide
survival
strategies.
Here,
we
will
describe
intricate
RNA-based
regulation
discuss
their
potential
biological
functions.
Language: Английский
The (p)ppGpp synthetase Rsh promotes rifampicin tolerant persister cell formation in Brucella abortus by regulating the type II toxin-antitoxin module mbcTA
Xiaofang Liu,
No information about this author
Pingping Wang,
No information about this author
Nanbo Yuan
No information about this author
et al.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: May 22, 2024
Persister
cells
are
transiently
tolerant
to
antibiotics
and
associated
with
recalcitrant
chronic
infections
due
recolonization
of
host
after
antibiotic
removal.
Brucella
spp.
facultative
pathogens
that
establish
intracellular
infection
cycles
in
which
results
persistent
infections.
abortus
forms
multi-drug
persister
promoted
by
the
(p)ppGpp
synthetase
Rsh
during
rifampicin
exposure.
Here,
we
confirmed
formation
B.
stationary
phase
treated
enrofloxacin.
Deletion
gene
for
decreased
level
presence
these
drugs
different
growth
phases.
However,
deletion
strain
varied
phases
other
antibiotics.
also
was
involved
treatment
under
certain
stress
conditions,
including
acidic
exposure
PBS,
heat
stress.
Moreover,
impacted
cell
levels
or
enrofloxacin
RAW264.7
macrophages.
Certain
typeIItoxin-antitoxin
modules
were
upregulated
various
conditions
.
We
established
positively
regulated
type
II
toxin-antitoxin
mbcTA
rifampicin-tolerant
elevated
ATP
when
promoter
overexpressed
background
phase.
Our
plays
a
key
role
persistence
may
serve
as
potent
novel
target
combination
development
new
therapeutic
approaches
prevention
strategies
treat
Language: Английский
(P)ppGpp synthetase Rel facilitates cellulose formation of biofilm by regulating glycosyltransferase in Brucella abortus
Xiao Fang Liu,
No information about this author
Pingping Wang,
No information about this author
Zheng Dong
No information about this author
et al.
International Journal of Biological Macromolecules,
Journal Year:
2025,
Volume and Issue:
unknown, P. 140022 - 140022
Published: Jan. 1, 2025
Language: Английский
What Is the Impact of Antibiotic Resistance Determinants on the Bacterial Death Rate?
Antibiotics,
Journal Year:
2025,
Volume and Issue:
14(2), P. 201 - 201
Published: Feb. 14, 2025
Objectives:
Antibiotic-resistant
bacteria
are
widespread,
with
resistance
arising
from
chromosomal
mutations
and
genes
located
in
the
chromosome
or
mobile
genetic
elements.
While
determinants
often
reduce
bacterial
growth
rates,
their
influence
on
death
under
bactericidal
antibiotics
remains
poorly
understood.
When
exposed
to
which
they
susceptible,
typically
undergo
a
two-phase
decline:
fast
initial
exponentially
decaying
phase,
followed
by
persistent
slow-decaying
phase.
This
study
examined
how
affect
rates
during
both
phases.
Methods:
We
analyzed
of
ampicillin-exposed
Escherichia
coli
populations
strains
sensitive
ampicillin
but
resistant
nalidixic
acid,
rifampicin,
both,
carrying
conjugative
plasmids
RN3
R702.
Results:
Single
mutants
acid
rifampicin
decayed
faster
than
cells
early
whereas
double-resistant
mutant
exhibited
prolonged
survival.
These
contrasting
impacts
suggest
epistatic
interactions
between
mutations.
Persistent-phase
for
did
not
differ
significantly
wild-type
cells.
In
contrast,
plasmid-carrying
displayed
distinct
dynamics:
R702
plasmid-bearing
showed
higher
persistent-phase
plasmid-free
cells,
while
lower
rates.
Conclusions:
Bactericidal
may
kill
other
more
effectively
Moreover,
epistasis
occur
when
different
same
cell,
impacting
potential
antibiotic
choice.
results
have
significant
implications
optimizing
eradication
protocols
clinical
settings,
as
well
animal
health
industrial
food
safety
management.
Language: Английский
Unlocking the enigma of phenotypic drug tolerance: Mechanisms and emerging therapeutic strategies
Alok Mishra,
No information about this author
Ritesh Thakare,
No information about this author
Bela G. Santani
No information about this author
et al.
Biochimie,
Journal Year:
2023,
Volume and Issue:
220, P. 67 - 83
Published: Dec. 31, 2023
Language: Английский
Serine/threonine protein kinase mediates rifampicin resistance in Brucella melitensis through interacting with ribosomal protein RpsD and affecting antioxidant capacity
mSystems,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 5, 2024
ABSTRACT
Brucellosis,
a
zoonotic
disease,
has
re-emerged
in
both
humans
and
animals,
causing
significant
economic
losses
globally.
Recently,
an
increasing
number
of
rifampicin-resistant
Brucella
strains
have
been
isolated
worldwide
without
detectable
mutations
known
antibiotic
resistance
genes.
Here,
this
study
identified
the
deletion
serine/threonine
protein
kinase
(STPK)
gene
B.
melitensis
as
efficient
trigger
for
rifampicin
using
bioinformatics
predictions,
transposon
mutant
library,
mutation
strains.
Notably,
absence
STPK
could
increase
expression
ribosomal
proteins
genes
involved
sulfur
metabolism
reduced
glutathione,
decrease
NADPH
oxidase
activity
NADP
+
/NADPH
ratio,
which
is
associated
with
antioxidant
capacity
.
Moreover,
co-immunoprecipitation
revealed
that
efficiently
interact
RpsD,
possibly
altering
translation
riboswitch
expression.
These
findings
demonstrate
mediates
by
regulating
to
counteract
reactive
oxygen
species
induced
rifampicin.
Furthermore,
approaches
developed
provide
platform
screening
new
spp.,
or
its
pathway
can
serve
potential
target
drug
development
against
spp.
IMPORTANCE
New
via
predictions
whole-genome
mechanisms
,
function
interaction
proteins.
Language: Английский