Frontiers in Plant Science,
Journal Year:
2023,
Volume and Issue:
13
Published: Feb. 9, 2023
Plant
growth-promoting
bacteria
(PGPB)
represent
an
eco-friendly
alternative
to
reduce
the
use
of
chemical
products
while
increasing
productivity
economically
important
crops.
The
emission
small
gaseous
signaling
molecules
from
PGPB
named
volatile
organic
compounds
(VOCs)
has
emerged
as
a
promising
biotechnological
tool
promote
biomass
accumulation
in
model
plants
(especially
Arabidopsis
thaliana)
and
few
crops,
such
tomato,
lettuce,
cucumber.
Rice
(Oryza
sativa)
is
most
essential
food
crop
for
more
than
half
world's
population.
However,
VOCs
improve
this
performance
not
yet
been
investigated.
Here,
we
evaluated
composition
effects
bacterial
on
growth
metabolism
rice.
First,
selected
isolates
(IAT
P4F9
E.1b)
that
increased
rice
dry
shoot
by
up
83%
co-cultivation
assays
performed
with
different
durations
time
(7
12
days).
Metabolic
profiles
co-cultivated
these
controls
(without
non-promoter
bacteria-1003-S-C1)
were
investigated
via1H
nuclear
magnetic
resonance.
analysis
identified
metabolites
(e.g.,
amino
acids,
sugars,
others)
differential
abundance
between
treatments
might
play
role
metabolic
pathways,
protein
synthesis,
signaling,
photosynthesis,
energy
metabolism,
nitrogen
assimilation,
involved
promotion.
Interestingly,
IAT
displayed
consistent
promotion
activity
also
able
increase
vivo.
Molecular
identification
sequencing
16S
rRNA
gene
E.1b
showed
higher
identity
Serratia
Achromobacter
species,
respectively.
Lastly,
volatilomes
two
other
(1003-S-C1
Escherichia
coli
DH5α)
through
headspace
solid-phase
microextraction
coupled
gas
chromatography-mass
spectrometry.
Compounds
belonging
classes,
benzenoids,
ketones,
alcohols,
sulfide,
alkanes,
pyrazines,
identified.
One
VOCs,
nonan-2-one,
was
validated
vitro
bioactive
compound
capable
promoting
growth.
Although
further
analyses
are
necessary
properly
elucidate
molecular
mechanisms,
our
results
suggest
potential
candidates
sources
bioproducts,
contributing
sustainable
agriculture.
Journal of Hazardous Materials,
Journal Year:
2024,
Volume and Issue:
470, P. 134231 - 134231
Published: April 6, 2024
Fungicides
are
used
worldwide
to
improve
crop
yields,
but
they
can
affect
non-target
soil
microorganisms
which
essential
for
ecosystem
functioning.
Microorganisms
form
complex
communities
characterized
by
a
myriad
of
interspecies
interactions,
yet
it
remains
unclear
what
extent
indirectly
affected
fungicides
through
biotic
interactions
with
sensitive
taxa.
To
quantify
such
indirect
effects,
we
fragmented
microbial
community
filtration
alter
and
compared
the
effect
fungicide
hymexazol
between
fractions
in
microcosms.
We
postulated
that
OTUs
would
exhibit
different
response
across
communities.
found
primarily
bacterial
fungal
were
responsible
more
than
75%
shifts
relative
abundance
dominant
after
exposure
an
agronomic
dose
hymexazol.
However,
these
effects
decreased
when
doses
increased.
Our
results
also
suggest
N-cycling
processes
as
ammonia
oxidation
be
impacted
application.
This
work
sheds
light
on
impact
underscores
need
higher-tier
risk
assessment.
ENVIRONMENTAL
IMPLICATION:
In
this
study,
novel
approach
based
fragmentation
determine
application
could
fungi
bacteria
interactions.
assess
off-target
microorganisms,
selected
hymexazol,
is
control
variety
plant
pathogens,
exposed
arable
recommended
field
rate,
well
higher
rates.
findings
show
at
least
hymexazol-impacted
affected,
therefore
emphasizing
importance
tiered
