Cytotoxicity assessment and antimicrobial effects of cell-free supernatants from probiotic lactic acid bacteria and yeast against multi-drug resistant Escherichia coli DOI
Mahdi Asghari Ozma,

Reza Ghotaslou,

Mohammad Asgharzadeh

et al.

Letters in Applied Microbiology, Journal Year: 2024, Volume and Issue: 77(9)

Published: Sept. 1, 2024

Abstract The antibacterial, antibiofilm, and cytotoxicity activity of cell-free supernatants (CFSs) from probiotics, including Lactobacillus plantarum, Bifidobacterium bifidum, Saccharomyces cerevisiae against multi-drug resistant Escherichia coli evaluated in current research. minimum inhibitory concentration (MIC) bactericidal (MBC) the CFSs were determined by analyzing inhibition zone formation using agar disk diffusion for antibacterial activity, microtiter plate biofilm analysis, auto-aggregation done. substances analyzed GC-MS. MTT assay on HEK293 cells investigated CFS’s influence cell viability. examined biofilm-related virulence genes, aggR fimH real-time polymerase chain reaction (real-time PCR). All had bacteriostatic effects. B. bifidum exhibited highest antibiofilm compared to others. L. S. produce 19, 16, 11 mm zones E. coli, respectively. GC-MS indicated that Hydroxyacetone, 3-Hydroxybutyric acid, Oxime-methoxy-phenyl-dominated CFSs, test demonstrated a viability rate over 90%. Statistically, adding all lowered relative expression both genes.

Language: Английский

Gut microbiota in neurological diseases: Melatonin plays an important regulatory role DOI Open Access
Somayeh Ahmadi, Mohammad Taghizadieh, Elham Mehdizadehfar

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 174, P. 116487 - 116487

Published: March 21, 2024

Melatonin is a highly conserved molecule produced in the human pineal gland as hormone. It known for its essential biological effects, such antioxidant activity, circadian rhythm regulator, and immunomodulatory effects. The gut one of primary sources melatonin. microbiota helps produce melatonin from tryptophan, has been shown to have beneficial effect on barrier function microbial population. Dysbiosis intestinal associated with bacterial imbalance decreased metabolites, including In this way, low levels may be related several diseases. both preventive therapeutic effects against various conditions, neurological diseases Alzheimer's disease, Parkinson's multiple sclerosis. This review was aimed discuss role body, describe possible relationship between production, well potential

Language: Английский

Citations

18
Gut Microbiota and Bacterial Translocation in the Pathogenesis of Liver Fibrosis DOI Open Access
Roman Maslennikov, Elena Poluektova, O. Yu. Zolnikova

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(22), P. 16502 - 16502

Published: Nov. 19, 2023

Cirrhosis is the end result of liver fibrosis in chronic diseases. Studying mechanisms its development and developing measures to slow down regress it based on this knowledge seem be important tasks for medicine. Currently, disorders gut–liver axis have great importance pathogenesis cirrhosis. However, gut dysbiosis, which manifests as increased proportions microbiota Bacilli Proteobacteria that are capable bacterial translocation a decreased proportion Clostridia strengthen intestinal barrier, occurs even at pre-cirrhotic stage disease. This leads translocation, process by those microbes enter blood portal vein then tissue, where they activate Kupffer cells through Toll-like receptor 4. In response, produce profibrogenic cytokines, hepatic stellate cells, stimulating their transformation into myofibroblasts collagen other elements extracellular matrix. Blocking with antibiotics, probiotics, synbiotics, methods could progression fibrosis. was shown number animal models but requires further verification long-term randomized controlled trials humans.

Language: Английский

Citations

28
Akkermansia muciniphila : A promising probiotic against inflammation and metabolic disorders DOI Creative Commons
Yanqing Zhao, Huijun Yang, Peng Wu

et al.

Virulence, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 27, 2024

Metabolic disease is a worldwide epidemic that has become public health problem. Gut microbiota considered to be one of the important factors maintain human by regulating host metabolism. As an abundant bacterium in gut,

Language: Английский

Citations

14
Rheum tanguticum polysaccharide alleviates DSS-induced ulcerative colitis and regulates intestinal microbiota in mice DOI
Yan Zhang, Ying Liu,

Jie Luo

et al.

Food Bioscience, Journal Year: 2023, Volume and Issue: 53, P. 102788 - 102788

Published: June 1, 2023

Language: Английский

Citations

21
Role of CRISPR-Cas systems and anti-CRISPR proteins in bacterial antibiotic resistance DOI Creative Commons
Hiva Kadkhoda, Pourya Gholizadeh, Hossein Samadi Kafil

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(14), P. e34692 - e34692

Published: July 1, 2024

The emergence and development of antibiotic resistance in bacteria is a serious threat to global public health. Antibiotic genes (ARGs) are often located on mobile genetic elements (MGEs). They can be transferred among by horizontal gene transfer (HGT), leading the spread drug-resistant strains treatment failure. CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated genes) one many strategies have developed under long-term selection pressure restrict HGT. CRISPR-Cas systems exist about half bacterial genomes play significant role limiting resistance. On other hand, bacteriophages MGEs encode wide range anti-CRISPR proteins (Acrs) counteract immunity system. Acrs could decrease system's activity against phages facilitate acquisition ARGs virulence traits for bacteria. This review aimed assess relationship between with We also highlighted technology control prevent antibacterial system target nucleic acid sequences high accuracy reliability; therefore, it has become novel editing therapy tool CRISPR-based approaches may pave way developing smart antibiotics, which eliminate multidrug-resistant (MDR) distinguish pathogenic beneficial microorganisms. Additionally, engineered gene-containing combination antibiotics used as cutting-edge approach reduce

Language: Английский

Citations

8
The Role of Phytochemicals and Gut Microbiome in Atherosclerosis in Preclinical Mouse Models DOI Open Access
Ann M. Centner, Leila Khalili, Vladimir Ukhanov

et al.

Nutrients, Journal Year: 2023, Volume and Issue: 15(5), P. 1212 - 1212

Published: Feb. 28, 2023

Gut microbiome alterations have recently been linked to many chronic conditions including cardiovascular disease (CVD). There is an interplay between diet and the resident gut microbiome, where food eaten affects populations of certain microbes. This important, as different microbes are associated with various pathologies, they can produce compounds that disease-promoting or disease-protecting. The Western negatively host ultimately resulting in heightened arterial inflammation cell phenotype changes well plaque accumulation arteries. Nutritional interventions whole foods rich fiber phytochemicals isolated polyphenols traditional medicinal plants show promise positively influencing alleviate atherosclerosis. review investigates efficacy a vast array on atherosclerotic burden mice. Reduction by was increases bacterial diversity, reduction Firmicutes/Bacteroidetes (F/B) ratio, upregulation Akkermansia. Upregulation CYP7 isoform liver, ABC transporters, bile acid excretion, level acetic acid, propionic butyric were also noted several studies reducing plaque. These attenuated oxidative stress. In conclusion, increase abundance Akkermansia diets polyphenols, fiber, grains likely reduce patients suffering from CVD.

Language: Английский

Citations

14
Exposure to chlorinated drinking water alters the murine fecal microbiota DOI Creative Commons
Jana Jandová,

Gabriele Schiro,

Frank A. Duca

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 914, P. 169933 - 169933

Published: Jan. 8, 2024

An abundant body of scientific studies and regulatory guidelines substantiates antimicrobial efficacy freshwater chlorination ensuring drinking water safety in large populations worldwide. In contrast to the purposeful use water, only a limited research has addressed molecular impact chlorinated exposure on gut microbiota. Here, for first time, we have examined differential effects regimens stratified by agent [inorganic (HOCl) versus chloramine (TCIC)] C57BL/6J murine fecal To this end, exposed mice followed bacterial microbiota analysis at end three-week feeding period employing 16S rRNA sequencing. α-diversity was strongly reduced when comparing control groups community dissimilarities (β-diversity) were significant between even HOCl TCIC. We detected differences composition as function observable phylum genus levels. Differential abundance select amplicon sequence variants (ASVs) revealed changes [up: Lactobacillus ASV1; Akkermansia muciniphila ASV7; Clostridium ss1 ASV10; down: Ileibacterium valens ASV5; Desulfovibrio ASV11; Lachnospiraceae UCG-006 ASV15]. Given established complexity human gastrointestinal their role health disease, translational relevance chlorination-induced documented us time remains be explored.

Language: Английский

Citations

5
Insoluble Dietary Fiber from Okara Combined with Intermittent Fasting Treatment Synergistically Confers Antiobesity Effects by Regulating Gut Microbiota and Its Metabolites DOI
Sainan Wang, Junyao Wang, Jiarui Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(36), P. 13346 - 13362

Published: Aug. 31, 2023

Insoluble dietary fiber (IDF) was recently revealed to have an antiobesity impact. However, the impact and potential mechanism of high-purity IDF derived from okara (HPSIDF) on obesity caused by a high-fat diet (HFD) remain unclear. Except for supplementation, intermittent fasting (IF) has attracted extensive interest as new strategy against obesity. Thus, we hypothesize that HPSIDF combined with IF treatment may be more effective in preventing In this study, synergistically alleviated HFD-induced dyslipidemia, impaired glucose homeostasis, systemic inflammation, fat accumulation. Furthermore, gut microbiota dysbiosis lowered short-chain fatty acid synthesis were recovered treatment. Meanwhile, metabolomic analysis feces obviously reversed alterations metabolic pathways differential metabolites induced HFD, which linked modulations microbiota. Collectively, our findings indicated great substantially enhance efficacy modulating its metabolites.

Language: Английский

Citations

12
Akkermansia muciniphila: a potential booster to improve the effectiveness of cancer immunotherapy DOI

Shiying Fan,

Zhengting Jiang,

Zhilin Zhang

et al.

Journal of Cancer Research and Clinical Oncology, Journal Year: 2023, Volume and Issue: 149(14), P. 13477 - 13494

Published: July 25, 2023

Language: Английский

Citations

11
Akkermansia muciniphila and herbal medicine in immune-related diseases: current evidence and future perspectives DOI Creative Commons
Xue Ding,

Pengfei Meng,

Xiuxia Ma

et al.

Frontiers in Microbiomes, Journal Year: 2024, Volume and Issue: 3

Published: Feb. 5, 2024

Akkermansia muciniphila is considered the “paradigm for next-generation beneficial microorganisms” and has been reported to help alleviat immune-related diseases. Evidence shows that herbal medicine can treat disease by regulating abundance of A. . Recent studies have revealed a link between Here, we systematically reviewed association , medicine, diseases (including inflammatory bowel disease, human immunodeficiency virus, cancer immunotherapy, liver injury). We also summarize potential mechanisms action offer perspectives future studies.

Language: Английский

Citations

4