El
género
Bordetella
está
compuesto
por
especies
que
pueden
causar
infecciones
respiratorias
en
diversos
organismos.
Entre
ellas
se
encuentra
pertussis,
un
patógeno
exclusivo
de
humanos
y
agente
causal
la
enfermedad
conocida
como
tos
convulsa.
Para
llevar
a
cabo
infección,
B.
pertussis
expresa
una
serie
factores
virulencia
encuentran
regulados
principalmente
actividad
del
sistema
dos
componentes
BvgAS.
Sin
embargo,
los
últimos
años
han
surgido
evidencias
demuestran
hay
otros
mecanismos
regulatorios
adicionales
involucrados
expresión
virulencia.
En
nuestro
laboratorio,
nos
encontramos
abocados
al
estudio
señalización
mediado
el
segundo
mensajero
c-di-GMP
su
rol
patogénesis
bacterias
Bordetella.
Los
niveles
célula
controlados
familias
proteínas:
diguanilato
ciclasas
fosfodiesterasas,
sintetizan
degradan
mensajero,
respectivamente.
Estas
proteínas
identificarse
genomas
bacterianos
debido
presencia
dominios
conservados:
GG(D/E)EF
para
las
EAL
o
HD-GYP
fosfodiesterasas.
este
contexto,
objetivo
presente
trabajo
tesis
es
evaluar
si
importante
control
procesos
asociados
pertussis.
ello,
centramos
particular:
BP1492,
proteína
con
dominio
GG(D/E)EF,
BP1092,
“dual”
posee
GGDEF,
histidina
quinasa.
A
través
utilización
diversas
técnicas
microbiología
biología
molecular
pudimos
evidenciar
BP1492
ciclasa
activa,
capaz
modificar
fenotipos
estaría
involucrada
resistencia
pH
ácido
Por
otra
parte,
describimos
mecanismo
local
entre
BP1092
ciclasa,
podría
regular
asociadas
conclusión,
obtuvieron
resultados
regula
podrían
estar
relacionados
realiza
aporte
comprensión
moleculares
regulatorio.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(10), P. 5368 - 5378
Published: Feb. 23, 2024
This
study
employed
adaptive
laboratory
evolution
to
improve
the
acid
tolerance
of
Lactiplantibacillus
plantarum,
a
vital
strain
in
food
fermentation
and
potential
probiotic.
Phenotype
genomic
analyses
identified
overexpression
stress
response
proteins,
ATP
synthases,
transporters
as
pivotal
conferring
evolved
strains.
These
adaptations
led
shorter
lag
phase,
improved
survival
rates,
higher
intracellular
pH
values
compared
wild-type
under
conditions.
Additionally,
strains
showed
an
increased
expression
genes
fatty
synthesis
pathway,
resulting
production
unsaturated
acids.
The
changes
cell
membrane
composition
possibly
prevented
H+
influx,
while
mutant
related
surface
structure
contributed
observed
elongated
cells
thicker
surface.
alterations
wall
composition,
along
with
transporter
efficiency,
were
key
factors
contributing
enhanced
Frontiers in Chemistry,
Journal Year:
2025,
Volume and Issue:
12
Published: Jan. 10, 2025
Cyclic
di-guanosine
monophosphate
(c-di-GMP)
acts
as
a
second
messenger
regulating
bacterial
behaviors
including
cell
cycling,
biofilm
formation,
adhesion,
and
virulence.
Monitoring
c-di-GMP
levels
is
crucial
for
understanding
these
processes
designing
inhibitors
to
combat
biofilm-related
antibiotic
resistance.
Here,
we
developed
genetically
encoded
biosensor,
cdiGEBS,
based
on
the
transcriptional
activity
of
c-di-GMP-responsive
transcription
factor
MrkH.
Notably,
cdiGEBS
can
detect
both
low
high
cellular
levels,
with
fluorescence
dynamic
change
23-fold.
Moreover,
it
subtle
changes
in
concentrations
due
variations
expression
synthesis
or
degradation
enzymes
distinguish
different
activities
among
WspR
mutants.
These
capabilities
allow
us
apply
identifying
new
diguanylate
cyclases
evaluating
chemicals
that
modulate
highlighting
its
potential
high-throughput
tool
screening
enzymes.
Overall,
enhances
study
c-di-GMP-regulated
functions
holds
antimicrobials
targeting
Proceedings of the National Academy of Sciences,
Journal Year:
2025,
Volume and Issue:
122(4)
Published: Jan. 22, 2025
Multicellularity
spans
a
wide
gamut
in
terms
of
complexity,
from
simple
clonal
clusters
cells
to
large-scale
organisms
composed
differentiated
and
tissues.
While
recent
experiments
have
demonstrated
that
forms
multicellularity
can
readily
evolve
response
different
selective
pressures,
it
is
unknown
if
continued
exposure
those
same
pressures
will
result
the
evolution
increased
multicellular
complexity.
We
use
mathematical
models
consider
adaptive
trajectories
unicellular
exposed
periodic
bouts
abiotic
stress,
such
as
drought
or
antibiotics.
Populations
improve
survival
stress
by
evolving
cell
differentiation—or
both;
however,
these
responses
associated
costs
when
absent.
define
parameter
space
fitness-relevant
traits
identify
where
multicellularity,
differentiation,
their
combination
fittest.
then
study
effects
adaptation
allowing
populations
fix
mutations
fitness.
find
while
mutation
be
beneficial
e.g.,
strict
unicellularity
life
cycles
with
stages
magnitudes
differ
alter
which
As
result,
we
observe
gain
lose
also
show
order
mutations,
historical
contingency,
cause
some
transitions
permanent
absence
neutral
evolution.
Ultimately,
driver
for
either
lead
increasing
complexity
return
unicellularity.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 23, 2025
Abstract
Many
bacteria
and
archaea
use
CRISPR-Cas
systems,
which
provide
RNA-based,
adaptive,
inheritable
immune
defenses
against
invading
viruses
other
foreign
genetic
elements.
The
proper
processing
of
CRISPR
guide
RNAs
(crRNAs)
is
a
crucial
step
in
the
maturation
defense
complexes
frequently
performed
by
specialized
ribonucleases
encoded
cas
genes.
However,
some
systems
employ
enzymes
associated
with
degradosome
or
housekeeping
functions,
such
as
RNase
III
endoribonuclease
E.
Here,
endo-
5′-exoribonuclease
J
was
identified
additional
enzyme
involved
crRNA
maturation,
acting
jointly
E
type
III-Bv
system,
possibly
together
further
RNase.
Co-IP
experiments
revealed
small
set
proteins
that
were
co-enriched
J,
among
them
PNPase.
Despite
measured,
strong
3’
exonucleolytic
activity
recombinant
enzyme,
PNPase
not
confirmed
to
contribute
maturation.
co-IP
results
indicate
component
cyanobacterial
can
recruit
either
enriched
proteins.
