Salinity Stress Resilience in Sorghum bicolor through Pseudomonas-Mediated Modulation of Growth, Antioxidant System, and Eco-Physiological Adaptations

ACS Omega, Journal Year: 2025, Volume and Issue: 10(1), P. 940 - 954

Published: Jan. 5, 2025

Increased soluble salts in soil and irrigation water threaten the sustainability of crops. This causes food insecurity directly by reducing staple crop yield indirectly limiting fodder forage production. Recently, plant-growth-promoting rhizosphere microorganism utilization improved productivity under stress. Therefore, this research was conducted to find Sorghum bicolor growth improvement potential exogenous application five different Pseudomonas strains salinity a pot experiment. The applied with 1/2-strength Hoagland's nutrient solution as 0 100 mM NaCl for 30 days. Results indicated that reduced vegetative parameters stress-responsive biochemicals nonbacterial treated plants. However, plants exhibited notable increases growth, relative content, antioxidant enzyme activities, osmolytes, photosynthetic pigments salinity. ionic imbalance also due improving K+ K+/Na+ ratios P. aeruginosa strain SAHK (OQ194056) putida AHK_SHA007 (OR468335) were found be promising compared other increasing stress tolerance. augmentation plant's system maintenance ion homeostasis served strategy enhance plant salt

Language: Английский

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PGPg_finder: A comprehensive and user-friendly pipeline for identifying plant growth-promoting genes in genomic and metagenomic data

Rhizosphere, Journal Year: 2024, Volume and Issue: 30, P. 100905 - 100905

Published: May 23, 2024

Language: Английский

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Comparative effects of biocontrol agent and pathogen on Nicotiana tabacum : insights into fungal-plant interactions

Plant Signaling & Behavior, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 16, 2025

Tobacco (Nicotiana tabacum) black shank disease, caused by Phytophthora nicotianae, is a significant threat to tobacco crops, leading severe economic losses. Prolonged use of agrochemicals control this disease has prompted the exploration eco-friendly biological strategies. This study investigated effects Trichoderma harzianum, biocontrol agent, on N. tabacum in comparison P. focusing growth, biomass, root morphology and anatomy, hormonal changes, osmotic regulation. T. harzianum significantly enhanced plant biomass accumulation, system development, physiological attributes such as photosynthetic pigment levels antioxidant enzyme activity. In contrast, nicotianae negatively impacted these parameters, inhibiting growth function. Notably, increased proline content induced resistance mechanisms, mitigating stress promoting overall health. These findings highlight potential sustainable solution for managing while improving crop productivity.

Language: Английский

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Peptide nucleic acid (PNA) clamps reduce amplification of host chloroplast and mitochondria rRNA gene sequences and increase detected diversity in 16S rRNA gene profiling analysis of oak-associated microbiota

Environmental Microbiome, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 28, 2025

Acquiring representative bacterial 16S rRNA gene community profiles in plant microbiome studies can be challenging due to the excessive co-amplification of host chloroplast and mitochondrial sequences that reduce counts plant-associated sequences. Peptide Nucleic Acid (PNA) clamps prevent this by blocking PCR primer binding or within amplified region non-target DNA stop function polymerase. Here, we applied a universal (p)PNA clamp newly designed mitochondria (m)PNA minimise amplification amplicon leaf, bark root tissue two oak species (Quercus robur Q. petraea). Adding PNA led an overall reduction 79%, 46% 99% tissues, respectively. This resulted average increase sequencing reads 72%, 35%, 17% bark, tissue, Moreover, diversity leaf increased, with number ASVs rising 105 samples 218 samples, In where contamination were low, alpha beta did not change, suggesting bias community. conclusion, study shows effectively improve assessment detected Quercus petraea studies.

Language: Английский

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Screening and Evaluation of Actinomycetes B-35 from Rhizosphere of Salvia miltiorrhiza Bunge and Its Biocontrol and Growth-Promoting Effects

Agronomy, Journal Year: 2025, Volume and Issue: 15(1), P. 102 - 102

Published: Jan. 1, 2025

Salvia miltiorrhiza Bunge (S. miltiorrhiza) is one of the most commonly used bulk herbs in China; however, root rot can seriously affect its quality and yields. To minimize use chemical pesticides for managing this plant fungal disease, biological control utilizing microbial bio-pesticides offers a promising alternative. This study aimed to enhance biocontrol resources available combating S. by isolating actinomycetes with antifungal activity from rhizosphere soil identifying preventive effect on rot. A total 35 were successfully screened miltiorrhizae. The strain B-35 strongest was out through antagonizing pathogen Fusarium solani miltiorrhiza, morphology 16S rRNA analysis. antagonistic fermentation filtrate crude extract could significantly destroy mycelium spores solani; mature rhizome reached 83.3%, number leaves, height biomass treatment group increased compared group. has certain application potential promotion sourced been demonstrated first time, potentially enhancing future crop production.

Language: Английский

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Harnessing the synergy of Urochloa brizantha and Amazonian Dark Earth microbiomes for enhanced pasture recovery

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 17, 2025

Language: Английский

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Alternaria alternata JTF001 Metabolites Recruit Beneficial Microorganisms to Reduce the Parasitism of Orobanche aegyptiaca in Tomato

Biology, Journal Year: 2025, Volume and Issue: 14(2), P. 116 - 116

Published: Jan. 23, 2025

Orobanche aegyptiaca is a holoparasitic weed that extracts water, nutrients, and growth regulators from host plants, leading to significant yield quality losses. Biocontrol microbial metabolites have been shown enhance plant resistance against parasitic yet the underlying mechanisms remain poorly understood. In this study, we investigated role of Alternaria alternata JTF001 (J1) in recruiting beneficial microbes tomato rhizosphere promoting establishment disease-suppressive microbiome. Pot experiments revealed J1 metabolite application significantly reduced O. parasitism. High-throughput sequencing full-length 16S rRNA genes ITS regions, along with vitro culture assays, demonstrated an increase abundance plant-beneficial bacteria, particularly Pseudomonas spp. The three candidate strains (zOTU_388, zOTU_533, zOTU_2335) showed 5.7-fold, 5.4-fold, 4.7-fold, respectively. These results indicate induce recruitment microbiome seedlings, effectively inhibiting Our findings suggest represent promising strategy for managing infestations through community modulation, offering implications sustainable agricultural practices.

Language: Английский

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Bio-inoculant consortium and organic amendment comprising plant bioactive extract increased maize yield by improving soil nutrient availability and mitigating pest damage

Plant and Soil, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Language: Английский

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Rhizosphere Growth-Promoting Fungi of Healthy Nicotiana tabacum L.: A Systematic Approach to Boosting Plant Growth and Drought Resistance

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 543 - 543

Published: Feb. 27, 2025

Drought, exacerbated by global warming, poses a significant threat to crop growth and productivity. This study identified strain of Trichoderma harzianum from the rhizosphere healthy Nicotiana tabacum L. plants evaluated its role in enhancing drought tolerance. The isolated effectively colonized plant roots promoted N. To investigate potential, T. was inoculated into under varying conditions, impact on growth, physiological responses, resilience assessed. Comprehensive analyses agronomic traits, parameters, enzyme activities, photosynthetic performance, osmoprotectant levels, membrane lipid peroxidation revealed that inoculation (light with harzianum, moderate severe treatments) systematically improved development resistance. These findings provide valuable insights lay foundation for developing innovative biofertilizers enhance tolerance sustainability.

Language: Английский

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Bacillus subtilis can promote cotton phenotype, yield, nutrient uptake and water use efficiency under drought stress by optimizing rhizosphere microbial community in arid area

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 227, P. 120784 - 120784

Published: March 5, 2025

Language: Английский

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