Silica Nanochannels as Nanoreactors for the Confined Synthesis of Ag NPs to Boost Electrochemical Stripping Chemiluminescence of the Luminol-O₂ System for the Sensitive Aptasensor

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(25), P. 10264 - 10273

Published: June 13, 2024

Herein, we, for the first time, synthesize silver nanoparticles (Ag NPs) within nanochannels of amino group-functionalized vertically ordered mesoporous silica films (NH2–VMSF) and investigate their coreaction accelerator role in luminol-dissolved oxygen (O2) electrochemical stripping chemiluminescence (ESCL) system. The synthesized Ag NPs are capable electrocatalytic reduction O2 to superoxide radicals, meanwhile, sliver ions (Ag+) electrochemically stripped from can promote amount luminol anion generating boosted ECL intensity This proposed NPs@NH2–VMSF on indium tin oxide electrode was applied construct ESCL aptasensor quantitative determination prostate-specific antigen (PSA), yielding a low detection limit [0.19 pg/mL (S/N = 3)] broad linear dynamic range (1 100 ng/mL). Furthermore, good analytical performance PSA serum with satisfactory recoveries relative standard deviation values is achieved by our developed aptasensor, rendering it convenient sensitive method clinical applications further broadening strategy techniques.

Language: Английский

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Nanogold amplified electrochemiluminescence/electrochemistry in bipolar silica nanochannel array for ultrasensitive detection of SARS-CoV-2 pseudoviruses

Talanta, Journal Year: 2024, Volume and Issue: 277, P. 126319 - 126319

Published: May 27, 2024

Language: Английский

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Sensitive Detection of Biomarker in Gingival Crevicular Fluid Based on Enhanced Electrochemiluminescence by Nanochannel-Confined Co3O4 Nanocatalyst

Biosensors, Journal Year: 2025, Volume and Issue: 15(1), P. 63 - 63

Published: Jan. 19, 2025

The sensitive detection of inflammatory biomarkers in gingival crevicular fluid (GCF) is highly desirable for the evaluation periodontal disease. Luminol-based electrochemiluminescence (ECL) immunosensors offer a promising approach fast and convenient biomarkers. However, luminol’s low ECL efficiency under neutral conditions remains challenge. This study developed an immunosensor by engineering immunorecognition interface on outer surface mesoporous silica nanochannel film (SNF) confining Co3O4 nanocatalyst within SNF nanochannels to improve luminol efficiency. was grown indium tin oxide (ITO) electrode using simple Stöber solution growth method. A successfully confined through situ electrodeposition, confirmed X-ray photoelectron spectroscopy (XPS) electrochemical measurements. demonstrated excellent electrocatalytic activity, effectively enhancing H2O2 oxidation boosting signal conditions. Using interleukin-6 (IL-6) as proof-of-concept demonstration, epoxy functionalization enabled covalent immobilization capture antibodies, forming specific interface. IL-6 binding induced immunocomplex formation, which reduced allowed quantitative detection. showed linear range from 1 fg mL−1 10 ng mL−1, with limit (LOD) 0.64 mL−1. It also good selectivity anti-interference capabilities, enabling successful artificial GCF samples.

Language: Английский

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Solid-Phase Electrochemiluminescence Enzyme Electrodes Based on Nanocage Arrays for Highly Sensitive Detection of Cholesterol

Biosensors, Journal Year: 2024, Volume and Issue: 14(8), P. 403 - 403

Published: Aug. 21, 2024

The convenient and sensitive detection of metabolites is great significance for understanding human health status drug development. Solid-phase electrochemiluminescence (ECL) enzyme electrodes show potential in metabolite based on the enzyme-catalyzed reaction product hydrogen peroxide (H2O2). Herein, a solid-phase ECL sensor was fabricated confined emitter an immobilized using electrostatic nanocage array, constructing platform cholesterol. cage nanochannel consists bipolar bilayer vertically aligned mesoporous silica film (bp-VMSF). upper layer bp-VMSF amino-modified, positively charged VMSF (p-VMSF), lower negatively (n-VMSF). most commonly used probe tris(bipyridine)ruthenium(II) (Ru(bpy)32+) fixed n-VMSF by adsorption from repulsion p-VMSF, generating significantly enhanced stable signals. successful preparation characterized scanning electron microscopy (SEM) electrochemical methods. After amino groups outer surface were derivatized with aldehyde, cholesterol oxidase (ChOx) molecules covalently immobilized. construction electrode cyclic voltammetry (CV) impedance spectroscopy (EIS). When corresponding substrate, cholesterol, present solution, signal Ru(bpy)32+ quenched H2O2, enabling high-sensitivity linear range detecting 0.05 mM to 5.0 mM, limit (LOD) 1.5 μM.

Language: Английский

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Construction of electrochemical immunosensors based on Au@MXene and Au@CuS nanocomposites for sensitive detection of carcinoembryonic antigen

Talanta, Journal Year: 2024, Volume and Issue: 283, P. 127147 - 127147

Published: Nov. 2, 2024

Language: Английский

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Electrochemical/Electrochemiluminescence Sensors Based on Vertically‐Ordered Mesoporous Silica Films for Biomedical Analytical Applications

ChemBioChem, Journal Year: 2024, Volume and Issue: unknown

Published: June 14, 2024

Abstract Vertically‐ordered mesoporous silica films (VMSF, also named as isoporous membranes) have shown tremendous potential in the field of electroanalytical sensors due to their unique features terms controllable and ultrasmall nanopores, high molecular selectivity permeability, mechanical stability. This review will present recent progress on biomedical analytical applications VMSF, focusing small biomolecules, diseases‐related biomarkers, drugs cancer cells. Finally, conclusions with developments future perspective VMSF relevant fields be envisioned.

Language: Английский

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Direct and Sensitive Electrochemical Determination of Total Antioxidant Capacity in Foods Using Nanochannel-Based Enrichment of Redox Probes

Molecules, Journal Year: 2024, Volume and Issue: 29(11), P. 2423 - 2423

Published: May 21, 2024

Simple and sensitive determination of total antioxidant capacity (TAC) in food samples is highly desirable. In this work, an electrochemical platform was established based on a silica nanochannel film (SNF)-modified electrode, facilitating fast analysis TAC colored samples. SNF grown low-cost readily available tin indium oxide (ITO) electrode. Fe3+-phenanthroline complex-Fe(III)(phen)3 applied as the probe, underwent chemical reduction to form Fe2+-phenanthroline complex-Fe(II)(phen)3 presence antioxidants. Utilizing oxidative voltage +1 V, chronoamperometry employed measure current generated by oxidation Fe(II)(phen)3, allowing for assessment As negatively charged displayed remarkable enrichment towards positively sensitivity detection can be significantly improved. When Trolox standard antioxidant, sensor demonstrated linear range from 0.01 μM 1 1000 μM, with limit (LOD) 3.9 nM. The performance better that conventional colorimetric method de 40 μM. Owing anti-interfering ability nanochannels, direct including coffee, tea, edible oils realized.

Language: Английский

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Carbon Nitride Nanosheets as an Adhesive Layer for Stable Growth of Vertically-Ordered Mesoporous Silica Film on a Glassy Carbon Electrode and Their Application for CA15-3 Immunosensor

Molecules, Journal Year: 2024, Volume and Issue: 29(18), P. 4334 - 4334

Published: Sept. 12, 2024

Vertically ordered mesoporous silica films (VMSF) are a class of porous materials composed ultrasmall pores and ultrathin perpendicular nanochannels, which attractive in the areas electroanalytical sensors molecular separation. However, VMSF easily falls off from carbonaceous electrodes thereby impacts their broad applications. Herein, carbon nitride nanosheets (CNNS) were served as an adhesive layer for stable growth on glassy electrode (GCE). CNNS bearing plentiful oxygen-containing groups can covalently bind with silanol VMSF, effectively promoting stability GCE surface. Benefiting numerous open nanopores modification VMSF's external surface carbohydrate antigen 15-3 (CA15-3)-specific antibody allows target-controlled transport electrochemical probes through internal yielding sensitive quantitative detection CA15-3 range 1 mU/mL to 1000 U/mL low limit 0.47 mU/mL. Furthermore, proposed VMSF/CNNS/GCE immunosensor is capable highly selective accurate determination spiked serum samples, offers simple effective strategy various practical biomarkers complicated biological specimens.

Language: Английский

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Highly Sensitive Electrochemical Determination of Butylated Hydroxyanisole in Food Samples Using Electrochemical-Pretreated Three-Dimensional Graphene Electrode Modified with Silica Nanochannel Film

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(7), P. 569 - 569

Published: March 25, 2024

The sensitive detection of antioxidants in food is essential for the rational control their usage and reducing potential health risks. A simple three-dimensional (3D) electrode integrated with an anti-fouling/anti-interference layer possesses great direct electrochemical samples. In this work, a 3D sensor was developed by integrating graphene (3DG) vertically ordered mesoporous silica film (VMSF), enabling highly common antioxidant, butylated hydroxyanisole (BHA), polarization employed to pre-activate 3DG (p3DG), enhancing its hydrophilicity. Using p3DG as supporting electrode, stable modification VMSF achieved using assisted self-assembly (EASA) method, without need any adhesive agents (VMSF/p3DG). Taking BHA model analyte, VMSF/p3DG demonstrated high sensitivity, due enrichment nanochannels, towards BHA. Electrochemical linear range 0.1 μM 5 from 150 low limit (12 nM). Owing fouling resistance anti-interference capabilities VMSF, constructed can be directly applied complex

Language: Английский

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Two-dimensional nanozyme nanoarchitectonics customized electrochemical bio diagnostics and lab-on-chip devices for biomarker detection

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 341, P. 103474 - 103474

Published: March 20, 2025

Language: Английский

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