Science,
Journal Year:
2016,
Volume and Issue:
351(6278), P. 1218 - 1222
Published: March 10, 2016
In
vivo
mapping
of
transcription-factor
binding
to
the
transcriptional
output
regulated
gene
is
hindered
by
probabilistic
promoter
occupancy,
presence
multiple
copies,
and
cell-to-cell
variability.
We
demonstrate
how
overcome
these
obstacles
in
lysogeny
maintenance
bacteriophage
lambda,
P(RM).
simultaneously
measured
concentration
lambda
repressor
CI
number
messenger
RNAs
(mRNAs)
from
P(RM)
individual
Escherichia
coli
cells,
used
a
theoretical
model
identify
stochastic
activity
corresponding
different
configurations.
found
that
switching
between
configurations
faster
than
mRNA
lifetime
copies
within
same
cell
act
independently.
The
simultaneous
quantification
transcription
factor
activity,
followed
analysis,
provides
tool
can
be
applied
other
genetic
circuits.
Science,
Journal Year:
2016,
Volume and Issue:
353(6301)
Published: June 25, 2016
Microglia
development
follows
a
stepwise
program
are
cells
that
defend
the
central
nervous
system.
However,
because
they
migrate
into
brain
during
development,
changes
undergo,
including
those
affect
gene
expression,
have
been
difficult
to
document.
Matcovitch-Natan
et
al.
transcriptionally
profiled
expression
and
analyzed
epigenetic
signatures
of
microglia
at
single-cell
level
in
early
postnatal
life
mice.
They
identified
three
stages
which
characterized
by
linked
with
chromatin
changes,
occurring
sync
developing
brain.
Furthermore,
showed
proper
is
affected
microbiome.
Science
,
this
issue
p.
789
Cell Reports,
Journal Year:
2015,
Volume and Issue:
13(12), P. 2653 - 2662
Published: Dec. 1, 2015
mRNA
is
thought
to
predominantly
reside
in
the
cytoplasm,
where
it
translated
and
eventually
degraded.
Although
nuclear
retention
of
has
a
regulatory
potential,
considered
extremely
rare
mammals.
Here,
explore
extent
metabolic
tissues,
we
combine
deep
sequencing
cytoplasmic
RNA
fractions
with
single-molecule
transcript
imaging
mouse
beta
cells,
liver,
gut.
We
identify
wide
range
protein-coding
genes
for
which
levels
spliced
polyadenylated
are
higher
nucleus
than
cytoplasm.
These
include
such
as
transcription
factor
ChREBP,
Nlrp6,
Glucokinase,
Glucagon
receptor.
demonstrate
that
can
efficiently
buffer
from
noise
emanates
transcriptional
bursts.
Our
study
challenges
view
transcripts
cytoplasm
reveals
role
dampening
gene
expression
noise.
