Abundances of transcripts, proteins, and metabolites in the cell cycle of budding yeast reveal coordinate control of lipid metabolism DOI Open Access
Heidi M. Blank, Ophelia Papoulas, Nairita Maitra

et al.

Molecular Biology of the Cell, Journal Year: 2020, Volume and Issue: 31(10), P. 1069 - 1084

Published: May 1, 2020

In several systems, including budding yeast, cell cycle-dependent changes in the transcriptome are well studied. contrast, few studies queried proteome during division. There is also little information about dynamic metabolites and lipids cycle. Here, authors present such for dividing yeast cells.

Language: Английский

The bidirectional relationship between metabolism and cell cycle control DOI Creative Commons

Frances F. Diehl,

Kiera M. Sapp, Matthew G. Vander Heiden

et al.

Trends in Cell Biology, Journal Year: 2023, Volume and Issue: 34(2), P. 136 - 149

Published: June 27, 2023

Language: Английский

Citations

25
Pioneering the future of cancer therapy: Deciphering the p53-ferroptosis nexus for precision medicine DOI
Daiha Shin, Jaewang Lee, Jong‐Lyel Roh

et al.

Cancer Letters, Journal Year: 2024, Volume and Issue: 585, P. 216645 - 216645

Published: Jan. 26, 2024

Language: Английский

Citations

10
Genome dilution by cell growth drives starvation-like proteome remodeling in mammalian and yeast cells DOI
Michael C. Lanz, Shuyuan Zhang, Matthew P. Swaffer

et al.

Nature Structural & Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Language: Английский

Citations

9
Starvation signals in yeast are integrated to coordinate metabolic reprogramming and stress response to ensure longevity DOI Creative Commons
Nianshu Zhang, Lu Cao

Current Genetics, Journal Year: 2017, Volume and Issue: 63(5), P. 839 - 843

Published: April 25, 2017

Studies on replicative and chronological aging in Saccharomyces cerevisiae have greatly advanced our understanding of how longevity is regulated all eukaryotes. Chronological lifespan (CLS) yeast defined as the age-dependent viability non-dividing cell populations. A number nutrient sensing signal transduction pathways (mainly TOR PKA) been shown to regulate CLS, yet it poorly understood starvation signals transduced via these lead CLS extension. Using reporters whose expressions are induced by glucose starvation, we screened majority 'signaling' mutants genome identified many genes that necessary for stress response. Subsequent analyses not only revealed novel regulators such GSK-3 ortholog Mck1, but also demonstrated transmitted SNF1/AMPK, PKC1 those negatively TOR/PKA, including Rim15, Yak1 Mck1 kinases, integrated enable metabolic reprogramming acquisition resistance. Coordinated ensures accumulation storage carbohydrates quiescent cells maintain viability. We provide new evidence Yak1, Rim15 kinases cooperate activate H2O2-scanvenging activities, thus limiting levels ROS entering quiescence. These findings support recent advances higher organisms flexibility balance between energetics resistance unifying principles Future work reveal switch response coordinated will help delineate molecular mechanisms shed insight into aging/anti-aging organisms.

Language: Английский

Citations

87
Targeted p53 on Small-Molecules-Induced Ferroptosis in Cancers DOI Creative Commons
Weifen Zhang,

Chengcheng Gai,

Dejun Ding

et al.

Frontiers in Oncology, Journal Year: 2018, Volume and Issue: 8

Published: Nov. 2, 2018

Ferroptosis is a type of programmed cell death characterized by the accumulation lipid reactive oxygen species (L-ROS) driven oxidative degeneration lipids in an iron-dependent manner. The mechanism which degradation drives ROS-ferroptosis involves metabolic dysfunctions that result impaired intracellular processes and ROS production. Recent studies have found p53 acts as positive regulator ferroptosis promoting directly regulates versatility cells favoring mitochondrial respiration, leading to ROS-mediated ferroptosis. In mild stress, protects survival via eliminating ROS; additionally, human colorectal cancer, antagonizes formation DPP4–p53 complex. short, mechanisms p53-mediated production underlying cellular response are poorly understood. context recent research results, indistinct roles on scrutinized understand tumor suppression.

Language: Английский

Citations

68
Heat Oscillations Driven by the Embryonic Cell Cycle Reveal the Energetic Costs of Signaling DOI Creative Commons
Jonathan Rodenfels, Karla M. Neugebauer, Jonathon Howard

et al.

Developmental Cell, Journal Year: 2019, Volume and Issue: 48(5), P. 646 - 658.e6

Published: Feb. 1, 2019

Language: Английский

Citations

67
On the Molecular Mechanisms Regulating Animal Cell Size Homeostasis DOI
Evgeny Zatulovskiy, Jan M. Skotheim

Trends in Genetics, Journal Year: 2020, Volume and Issue: 36(5), P. 360 - 372

Published: Feb. 20, 2020

Language: Английский

Citations

64
G 1 cyclin–Cdk promotes cell cycle entry through localized phosphorylation of RNA polymerase II DOI
Mardo Kõivomägi, Matthew P. Swaffer, Jonathan J. Turner

et al.

Science, Journal Year: 2021, Volume and Issue: 374(6565), P. 347 - 351

Published: Oct. 15, 2021

Control by RNA polymerase II Evidence indicates that yeast cells initiate DNA synthesis and transition from the G 1 to S phase of cell cycle when cyclin 3 accumulates causes phosphorylation Whi5, a functional equivalent mammalian Rb (retinoblastoma) protein. Kõivomägi et al . now present evidence for different cyclin-dependent kinase target (see Perspective Fisher). They found 3–cyclin-dependent (Cdk) complex in promoted thus increased transcription at genes control entry into cycle. Cdks regulate can act similar mechanisms so-called “transcriptional Cdks,” which are known as transcriptional regulators but not function division. —LBR

Language: Английский

Citations

56
The multifaceted role of cell cycle regulators in the coordination of growth and metabolism DOI Creative Commons
Katharina Huber, Alberto Mestres‐Arenas, Lluís Fajas

et al.

FEBS Journal, Journal Year: 2020, Volume and Issue: 288(12), P. 3813 - 3833

Published: Oct. 8, 2020

Adapting to changes in nutrient availability and environmental conditions is a fundamental property of cells. This adaptation requires multi‐directional coordination between metabolism, growth, the cell cycle regulators (consisting family cyclin‐dependent kinases (CDKs), their regulatory subunits known as cyclins, CDK inhibitors, retinoblastoma members, E2F transcription factors). Deciphering mechanisms accountable for this crucial understanding various patho‐physiological processes. While it well established that metabolism growth affect division, review will focus on recent observations demonstrate how coordinate progression, growth. We discuss directly regulate metabolic enzymes pathways summarize involvement endolysosomal pathway functions dynamics mitochondria.

Language: Английский

Citations

53
Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle DOI Creative Commons
Vakil Takhaveev,

Serdar Özsezen,

Edward Smith

et al.

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(2), P. 294 - 313

Published: Feb. 27, 2023

Many cell biological and biochemical mechanisms controlling the fundamental process of eukaryotic division have been identified; however, temporal dynamics biosynthetic processes during cycle are still elusive. Here, we show that key temporally segregated along cycle. Using budding yeast as a model single-cell methods to dynamically measure metabolic activity, observe two peaks in protein synthesis, G1 S/G2/M phase, whereas lipid polysaccharide synthesis only once, phase. Integrating inferred rates into thermodynamic-stoichiometric model, find this segregation causes flux changes primary metabolism, with an acceleration glucose-uptake phase-shifted oscillations oxygen carbon dioxide exchanges. Through experimental validation predictions, demonstrate metabolism oscillates cell-cycle periodicity satisfy changing demands exhibiting unexpected

Language: Английский

Citations

21