CircRNA microarray profiling identifies a novel circulating biomarker for detection of gastric cancer DOI Creative Commons

Weiwei Tang,

Kai Fu, Handong Sun

et al.

Molecular Cancer, Journal Year: 2018, Volume and Issue: 17(1)

Published: Sept. 20, 2018

CircRNA expression profiles for gastric cancer (GC) were screened using plasma samples from 10 GC patients with different TNM stages and 5 healthy individuals as controls. Results showed lower of circ-KIAA1244 in tissues, plasmas, cells compare to normal Further clinical data analysis demonstrated that a decreased plasmas was negatively correlated stage lymphatic metastasis, shorter overall survival time patients. Moreover, we found could be detected exosomes no obvious significance compared the level corresponding plasmas. This study revealed GC-tissues-derived serve novel circulating biomarker detection GC.

Language: Английский

The biogenesis, biology and characterization of circular RNAs DOI
Lasse S. Kristensen,

Maria Schertz Andersen,

Lotte Victoria Winther Stagsted

et al.

Nature Reviews Genetics, Journal Year: 2019, Volume and Issue: 20(11), P. 675 - 691

Published: Aug. 8, 2019

Language: Английский

Citations

3660
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1 DOI Creative Commons

Daniel J. Klionsky,

Amal Kamal Abdel‐Aziz, Sara Abdelfatah

et al.

Autophagy, Journal Year: 2021, Volume and Issue: 17(1), P. 1 - 382

Published: Jan. 2, 2021

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered field. Our knowledge base relevant new technologies also been expanding. Thus, it is important to formulate on a regular basis updated monitoring autophagy different organisms. Despite numerous reviews, there continues be confusion regarding acceptable methods evaluate autophagy, especially multicellular eukaryotes. Here, present investigators select interpret examine related processes, reviewers provide realistic reasonable critiques reports that are focused these processes. These not meant dogmatic rules, because appropriateness any assay largely depends question being asked system used. Moreover, no individual perfect every situation, calling use multiple techniques properly monitor each experimental setting. Finally, several core components machinery implicated distinct autophagic processes (canonical noncanonical autophagy), implying genetic approaches block should rely targeting two or more autophagy-related genes ideally participate steps pathway. Along similar lines, proteins involved regulate other cellular pathways including apoptosis, all them can used as specific marker bona fide responses. critically discuss current assessing information they can, cannot, provide. ultimate goal encourage intellectual technical innovation

Language: Английский

Citations

1992
The expanding regulatory mechanisms and cellular functions of circular RNAs DOI
Ling‐Ling Chen

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(8), P. 475 - 490

Published: May 4, 2020

Language: Английский

Citations

1180
Circular RNA circNRIP1 acts as a microRNA-149-5p sponge to promote gastric cancer progression via the AKT1/mTOR pathway DOI Creative Commons
Xing Zhang, Sen Wang, Haixiao Wang

et al.

Molecular Cancer, Journal Year: 2019, Volume and Issue: 18(1)

Published: Feb. 4, 2019

CircRNA has emerged as a new non-coding RNA that plays crucial roles in tumour initiation and development. 'MiRNA sponge' is the most reported role played by circRNAs many tumours. The AKT/mTOR axis classic signalling pathway cancers sustains energy homeostasis through production activities, such Warburg effect, blocks catabolic autophagy. Additionally, exerts positive effect on EMT, which promotes metastasis. We detected higher circNRIP1 expression gastric cancer performing RNA-seq analysis. verified promotor of cells series biological function assays. then used pull-down assay dual-luciferase reporter to identify downstream miR-149-5p circNRIP1. Western blot analysis immunofluorescence assays were performed demonstrate circNRIP1-miR-149-5p-AKT1/mTOR responsible for altered metabolism GC adopted co-culture system trace transmission via exosomal communication RIP experiments determine quaking regulates expression. Finally, we confirmed suppressor microRNA-133a-3p vivo PDX mouse models. discovered knockdown successfully blocked proliferation, migration, invasion level AKT1 cells. MiR-149-5p inhibition phenocopied overexpression cells, malignant behaviours Moreover, it was proven can be transmitted between promoted metastasis vivo. also demonstrated promote transcription. In final step, proved sponges affect eventually acts GC.

Language: Английский

Citations

695
Structure and Degradation of Circular RNAs Regulate PKR Activation in Innate Immunity DOI Creative Commons

Chu‐Xiao Liu,

Xiang Li, Fang Nan

et al.

Cell, Journal Year: 2019, Volume and Issue: 177(4), P. 865 - 880.e21

Published: April 25, 2019

Language: Английский

Citations

660
Circular RNAs: Characterization, cellular roles, and applications DOI Creative Commons

Chu‐Xiao Liu,

Ling‐Ling Chen

Cell, Journal Year: 2022, Volume and Issue: 185(12), P. 2016 - 2034

Published: May 17, 2022

Language: Английский

Citations

635
The interplay between m6A RNA methylation and noncoding RNA in cancer DOI Creative Commons
Shuai Ma, Chen Chen, Xiang Ji

et al.

Journal of Hematology & Oncology, Journal Year: 2019, Volume and Issue: 12(1)

Published: Nov. 22, 2019

Abstract N6-methyladenosine (m6A) methylation, one of the most common RNA modifications, has been reported to execute important functions that affect normal life activities and diseases. Most studies have suggested m6A modification can complexity cancer progression by regulating biological related cancer. M6A noncoding RNAs regulates cleavage, transport, stability, degradation themselves. It also cell proliferation metastasis, stem differentiation, homeostasis in affecting function cells. Interestingly, play significant roles these modifications. Additionally, it is becoming increasingly clear potentially contribute clinical application treatment. In this review, we summarize effect interactions between modifications on involved progression. particular, discuss role as possible potential biomarkers therapeutic targets treatment cancers.

Language: Английский

Citations

519
circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity DOI Creative Commons

Botai Li,

Lili Zhu,

Chunlai Lu

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Jan. 12, 2021

Abstract Circular RNAs (circRNA) are a class of covalently closed single-stranded that have been implicated in cancer progression. Here we identify circNDUFB2 to be downregulated non-small cell lung (NSCLC) tissues, and negatively correlate with NSCLC malignant features. Elevated inhibits growth metastasis cells. Mechanistically, functions as scaffold enhance the interaction between TRIM25 IGF2BPs, positive regulator tumor progression metastasis. This TRIM25/circNDUFB2/IGF2BPs ternary complex facilitates ubiquitination degradation this effect enhanced by N 6 -methyladenosine (m A) modification . Moreover, is also recognized RIG-I activate RIG-I-MAVS signaling cascades recruit immune cells into microenvironment (TME). Our data thus provide evidences participates IGF2BPs activation anti-tumor immunity during via modulation both protein degradation, well cellular responses.

Language: Английский

Citations

440
The novel roles of circRNAs in human cancer DOI Creative Commons

Qingfeng Shang,

Zhi Yang, Renbing Jia

et al.

Molecular Cancer, Journal Year: 2019, Volume and Issue: 18(1)

Published: Jan. 9, 2019

Covalently closed single-stranded circular RNAs (circRNAs) consist of introns or exons and are widely present in eukaryotic cells. CircRNAs generally have low expression levels relatively stable structures compared with messenger (mRNAs), most which located the cytoplasm often act cell type tissue-specific manners, indicating that they may serve as novel biomarkers. In recent years, circRNAs gradually become a hotspot field RNA cancer research, but functions not yet been discovered. Known can affect biogenesis cancers diverse ways, such functioning microRNA (miRNA) sponges, combining binding proteins (RBPs), working transcription factor translation proteins. this review, we summarize characteristics types circRNAs, introduce discuss emerging databases on current challenges studies.

Language: Английский

Citations

429
Trends in insulin resistance: insights into mechanisms and therapeutic strategy DOI Creative Commons

Mengwei Li,

Xiaowei Chi, Ying Wang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2022, Volume and Issue: 7(1)

Published: July 6, 2022

The centenary of insulin discovery represents an important opportunity to transform diabetes from a fatal diagnosis into medically manageable chronic condition. Insulin is key peptide hormone and mediates the systemic glucose metabolism in different tissues. resistance (IR) disordered biological response for stimulation through disruption molecular pathways target Acquired conditions genetic factors have been implicated IR. Recent biochemical studies suggest that dysregulated metabolic mediators released by adipose tissue including adipokines, cytokines, chemokines, excess lipids toxic lipid metabolites promote IR other associated with several groups abnormal syndromes include obesity, diabetes, dysfunction-associated fatty liver disease (MAFLD), cardiovascular disease, polycystic ovary syndrome (PCOS), abnormalities. Although no medication specifically approved treat IR, we summarized lifestyle changes pharmacological medications used as efficient intervention improve sensitivity. Ultimately, systematic discussion complex mechanism will help identify potential new targets closely

Language: Английский

Citations

420