Cells,
Journal Year:
2019,
Volume and Issue:
8(11), P. 1379 - 1379
Published: Nov. 2, 2019
The
ribosome
is
a
complex
ribonucleoprotein-based
molecular
machine
that
orchestrates
protein
synthesis
in
the
cell.
Both
ribosomal
RNA
and
proteins
can
be
chemically
modified
by
reactive
oxygen
species,
which
may
alter
ribosome′s
functions
or
cause
complete
loss
of
functionality.
oxidative
damage
ribosomes
accumulate
during
their
lifespan
cell
lead
to
reduced
faulty
translation
contribute
various
pathologies.
However,
remarkably
little
known
about
biological
consequences
ribosome.
Here,
we
provide
concise
summary
types
changes
induced
species
rRNA
discuss
existing
experimental
evidence
how
these
modifications
affect
dynamics
function.
We
emphasize
special
role
redox-active
transition
metals,
such
as
iron,
play
homeostasis
stability.
also
hypothesis
redox-mediated
adaptive
cellular
responses
stress.
Non-Coding RNA,
Journal Year:
2019,
Volume and Issue:
5(1), P. 16 - 16
Published: Feb. 4, 2019
The
advent
of
RNA-sequencing
(RNA-Seq)
technologies
has
markedly
improved
our
knowledge
and
expanded
the
compendium
small
non-coding
RNAs,
most
which
derive
from
processing
longer
RNA
precursors.
In
this
review
article,
we
will
present
a
nonexhaustive
list
referenced
RNAs
(ncRNAs)
derived
eukaryotic
ribosomal
(rRNA),
called
rRNA
fragments
(rRFs).
We
focus
on
rRFs
that
are
experimentally
verified,
discuss
their
origin,
length,
structure,
biogenesis,
association
with
known
regulatory
proteins,
potential
role(s)
as
regulator
gene
expression.
This
relatively
new
class
ncRNAs
remained
poorly
investigated
underappreciated
until
recently,
due
mainly
to
priori
exclusion
sequences-because
overabundance-from
RNA-Seq
datasets.
situation
surrounding
resembles
microRNAs
(miRNAs),
used
be
readily
discarded
further
analyses,
for
more
than
five
decades,
because
no
one
could
believe
such
short
length
bear
biological
significance.
As
if
had
not
yet
learned
lesson
restrain
investigative,
scientific
mind
challenging
widely
accepted
beliefs
or
dogmas,
looking
hidden
treasures
in
unexpected
places.
Cell Reports,
Journal Year:
2018,
Volume and Issue:
25(1), P. 236 - 248.e6
Published: Oct. 1, 2018
Highlights•Nutrient
limitation
upregulates
the
relative
expression
of
rDNA
operon,
rrnH•Ribosomes
bearing
16S
rRNA,
rrsH,
can
modulate
general
stress
response•rrsH-bearing
ribosomes
impact
drug
resistance,
cell
motility,
and
biofilm
formation•Naturally
occurring
rRNA
sequence
variation
encodes
altered
ribosome
functionSummaryPrevailing
dogma
holds
that
are
uniform
in
composition
function.
Here,
we
show
nutrient
limitation-induced
E.
coli
changes
operons
to
alter
within
actively
translating
pool.
The
most
upregulated
operon
unique
distinguished
by
conserved
small
ribosomal
subunit.
rrsH-bearing
affect
functionally
coherent
gene
sets
levels
RpoS
sigma
factor,
master
regulator
response.
These
impacts
associated
with
phenotypic
antibiotic
sensitivity,
formation,
motility
regulated
response
proteins,
RelA
RelE,
as
well
metabolic
enzyme
virulence-associated
protein,
AdhE.
findings
establish
endogenously
encoded,
naturally
function,
central
aspects
regulation,
cellular
physiology.Graphical
abstract
Biomolecules,
Journal Year:
2018,
Volume and Issue:
8(4), P. 123 - 123
Published: Oct. 24, 2018
Ribosomal
RNAs,
the
most
abundant
cellular
RNA
species,
have
evolved
as
structural
scaffold
and
catalytic
center
of
protein
synthesis
in
every
living
organism.
In
eukaryotes,
they
are
produced
from
a
long
primary
transcript
through
an
intricate
sequence
processing
steps
that
include
cleavage
folding
nucleotide
modification.
The
mechanisms
underlying
this
process
human
cells
been
investigated,
but
technological
advances
accelerated
their
study
past
decade.
addition,
association
congenital
diseases
to
defects
ribosome
has
highlighted
central
place
ribosomal
maturation
cell
physiology
regulation
broadened
interest
these
mechanisms.
Here,
we
give
overview
current
knowledge
pre-ribosomal
light
recent
progress
discuss
how
dysfunction
pathway
may
contribute
physiopathology
diseases.
Cells,
Journal Year:
2019,
Volume and Issue:
8(11), P. 1379 - 1379
Published: Nov. 2, 2019
The
ribosome
is
a
complex
ribonucleoprotein-based
molecular
machine
that
orchestrates
protein
synthesis
in
the
cell.
Both
ribosomal
RNA
and
proteins
can
be
chemically
modified
by
reactive
oxygen
species,
which
may
alter
ribosome′s
functions
or
cause
complete
loss
of
functionality.
oxidative
damage
ribosomes
accumulate
during
their
lifespan
cell
lead
to
reduced
faulty
translation
contribute
various
pathologies.
However,
remarkably
little
known
about
biological
consequences
ribosome.
Here,
we
provide
concise
summary
types
changes
induced
species
rRNA
discuss
existing
experimental
evidence
how
these
modifications
affect
dynamics
function.
We
emphasize
special
role
redox-active
transition
metals,
such
as
iron,
play
homeostasis
stability.
also
hypothesis
redox-mediated
adaptive
cellular
responses
stress.
