Investigation into Potential Allergenicity and Digestion-Resistant Linear Epitopes of Fish Skin Gelatin in Cell-Cultured Meat Scaffolds

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(26), P. 14922 - 14940

Published: June 17, 2024

As a key component of cell-cultured fish, fish skin gelatin (FSG)-based cell scaffold provides support structures for growth, proliferation, and differentiation. However, there are potential allergenicity risks contained in FSG-based scaffolds. In this study, 3D edible scaffolds were prepared by phase separation method showed contact angle less than 90°, which indicated that the favorable adhesion. Besides, swelling ratio was greater 200%, implying great to growth. The sequence homology analysis FSG prone cross-reaction with collagen analogues. Additionally, food allergic model constructed represented mice gavaged cod exhibited higher levels specific antibodies, mast degranulation, vascular permeability, intestinal barrier impairment those pangasius tilapias FSG. Its might be attributed number digestion-resistant linear epitopes. Moreover, hydrolysis degree linked exposure epitopes promote combination IgE, also responsible maintaining This study clarifies allergenic further will focus on reduction

Language: Английский

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The interplay between the microbiota, diet and T regulatory cells in the preservation of the gut barrier in inflammatory bowel disease

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 1, 2023

Inflammatory bowel disease (IBD) is becoming more common in the Western world due to changes diet-related microbial dysbiosis, genetics and lifestyle. Incidences of gut permeability can predate IBD continued barrier disruptions increase exposure bacterial antigens immune system thereby perpetuating chronic inflammation. Currently, most approved therapies target individual pro-inflammatory cytokines pathways. However, they fail approximately 50% patients their inability overcome redundant pro inflammatory responses. There increasing interest therapeutic potential T regulatory cells (Tregs) conditions widespread capability dampen inflammation, promote tolerance intestinal bacteria, facilitate healing mucosal ability be engineered for targeted therapy. Intestinal Treg populations are inherently shaped by dietary molecules microbiota-derived metabolites. Thus, understanding how these influence Treg-mediated preservation will provide insights into tolerance-mediated homeostasis. This review comprehensively explores interplay between diet, microbiota, influencing function attenuate progression colitis.

Language: Английский

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A comparative study on the allergenic potential of β-lactoglobulin conjugated to glucose, caffeic acid and caffeoyl glucopyranose

Food & Function, Journal Year: 2023, Volume and Issue: 14(9), P. 4354 - 4367

Published: Jan. 1, 2023

This work intends to perform a comparative study on the allergenic potential of β-lactoglobulin (BLG)-glucose, BLG-caffeic acid and BLG-caffeoyl glucopyranose conjugates. The modifications changed molecular weight multi-structure BLG destroyed epitope, which resulted in decrease IgE binding level release ability histamine IL-6 KU812 cells. Compared with BLG, conjugates reduced serum levels IgG, IgE, β-Hex IL-4 vivo, while increasing interferon-γ, caused an imbalance Th1/Th2 immune response. Meanwhile, these not only increased relative abundance allergy-related gut flora, such as Lachnospiraceae, norank_o_Clostridia_UCG-014, Erysipelotrichaceae, Turicibacter Lachnospiraceae_NK4A136_group, but also improved short-chain fatty acids (SCFAs). Caffeoyl large long carbon chains exerted great influence flora SCFAs. Therefore, changes balance SCFA produced by were responsible for reducing allergy BLG.

Language: Английский

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Living on the edge: Mucus-associated microbes in the colon

Mucosal Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

The colonic mucus layer acts as a physicochemical barrier to pathogen invasion and habitat for mucus-associated microbes. This mucosal microbiome plays crucial role in moderating production, maintaining integrity, shaping the host immune response. However, unchecked mucin foraging may render vulnerable disease. To better understand these dynamics layer, it is essential advance fundamental knowledge on how commensals bind utilize well their interactions with both microbial neighbors. We present an overview of approaches surveying bacteria assessing mucin-utilizing capacity, alongside discussion limitations existing methods. Additionally, we highlight diet secretory immunoglobulin A interact bacterial community colon. Insights into this subset can guide therapeutic strategies optimally support modulate integrity.

Language: Английский

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Critical features of an in vitro intestinal absorption model to study the first key aspects underlying food allergen sensitization

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2022, Volume and Issue: 22(2), P. 971 - 1005

Published: Dec. 22, 2022

Abstract New types of protein sources will enter our diet in a near future, reinforcing the need for straightforward vitro (cell‐based) screening model to test and predict safety these novel proteins, particular their potential risk de novo allergic sensitization. The Adverse Outcome Pathway (AOP) allergen sensitization describes current knowledge key events underlying complex cellular interactions that proceed food Currently, there is no consensus on study intestinal translocation proteins as well epithelial activation, which comprise first molecular initiation (ME1‐3) event AOP, respectively. As members INFOGEST, we have highlighted several critical features should be considered any proposed transport context In addition, defined cell are indispensable steps optional or desired when possibility create more model. A aspects AOP can used gut behavior known hypo‐ hyperallergens, juxtaposed screen management dietary proteins. Indeed, this disquisition forms basis development future cascade, comprising also other (KE2‐5).

Language: Английский

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Soybean glycinin and β-conglycinin damage the intestinal barrier by triggering oxidative stress and inflammatory response in weaned piglets

European Journal of Nutrition, Journal Year: 2023, Volume and Issue: 62(7), P. 2841 - 2854

Published: June 26, 2023

Language: Английский

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Oral Exposure to Clostridium difficile Toxin A Aggravates Food Allergy by Intestinal Barrier Destruction, Mast Cell Activation, and Th2-Biased Immune Responses

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Food allergy is a complex disease, with multiple environmental factors involved. Considering the regulatory effect of Clostridium difficile toxin A (Tcd A) on biological processes allergic reactions, role oral exposure to Tcd food was investigated. The intestinal permeability and β-hexosaminidase were promoted by using in vitro Caco-2 HT-29 cells coculture monolayer bone marrow-derived mast cell (MCs) degranulation model. When sensitized mice challenged OVA 4 μg A, specific IgE MCPT-1 levels increased. results flow cytometry ELISA demonstrated that Th1 Treg reduced higher IL-5 induced. jejunum staining showed villus injury MC accumulation aggravated. All findings could aggravate allergy, orally exposed risk aggravating warrant increasing attention.

Language: Английский

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Oral administration of Lactobacillus rhamnosus MP108 ameliorates hemolytic jaundice in rats

Journal of Food Science, Journal Year: 2025, Volume and Issue: 90(2)

Published: Feb. 1, 2025

Accumulating evidence suggests that specific probiotic strains exert effects on hemolytic jaundice (HJ), and of Lactobacillus rhamnosus exhibit potential beneficial against HJ. This study aimed to determine the L. MP108 (MP108) rats with acetylphenylhydrazine (APH)-induced One week oral administration (16 × 109 CFU/kg·day) significantly reversed HJ-induced body-weight reduction normalized serum levels alanine aminotransferase (ALT), aspartate (AST), total bilirubin (TBIL), superoxide dismutase (T-SOD), glutathione peroxidase (GSH-PX), hydrogen peroxide (H2O2), anion (O2-) in HJ rats. Furthermore, significant improvements were observed pathological changes liver intestinal tissues. 16S rRNA high-throughput sequencing fecal samples demonstrated altered gut microbiota composition by increasing Lacticaseibacillus spp. abundance, which correlated ALT, AST, TBIL, T-SOD, GSH-PX, H2O2, O2-. In summary, these results provide has improve symptoms alleviating hepatic impairment, is associated composition. PRACTICAL APPLICATION: The indicates can modulate microbiota, function, thereby alleviate (HJ). These findings suggest a promising therapeutic approach for HJ, offering benefits patients related conditions.

Language: Английский

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Fucoidan-Mediated Covalent Modification Induces Oral Tolerance to Shrimp by Generating Tolerogenic Peptides and Reducing Antigen Responsiveness

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(9), P. 5548 - 5561

Published: Feb. 19, 2025

Food allergy has become a global food safety issue, and inducing tolerance of the immune system to allergens is seen as an effective way address this problem. In study, shrimp (Penaeus vannamei) was covalently modified with fucoidan explore its potential oral inducer. The results showed that strategy not only had no adverse effect on growth mice but also achieved significant induction effects. Specifically, it significantly reduced specific antibody levels, improved vascular permeability intestinal barrier function, inhibited mast cell degranulation. Further studies these positive were related tolerogenic peptides (SLLKANIQL, GLTEFQAV, GDFPGAFKVF, ALNLNPTLALI, AALDIDSKPF) produced in allergens. Moreover, mainly down-regulated gene expression exogenous substance metabolic immune-related signaling pathways, thereby reducing response antigens. Overall, fucoidan-mediated covalent modification promises be efficient method for producing inducers.

Language: Английский

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How fish intestinal cells responded to dietary methylmercury exposure? A single-cell transcriptomic analysis

Environmental Pollution, Journal Year: 2025, Volume and Issue: 371, P. 125967 - 125967

Published: March 3, 2025

Language: Английский

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