Extracellular vesicle-mediated approaches for the diagnosis and therapy of MASLD: current advances and future prospective DOI Creative Commons

Swasthika Gurjar,

R. A.,

Raghavendra Upadhya

et al.

Lipids in Health and Disease, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 7, 2025

Abstract Metabolic dysfunction-associated steatotic liver disease (MASLD) is an asymptomatic, multifaceted condition often associated with various risk factors, including fatigue, obesity, insulin resistance, metabolic syndrome, and sleep apnea. The increasing burden of MASLD underscores the critical need for early diagnosis effective therapies. Owing to lack efficient therapies MASLD, crucial. Consequently, noninvasive biomarkers imaging techniques are essential analyzing play a pivotal role in global diagnostic process. use extracellular vesicles has emerged as promising therapy ailments. Herein, comprehensive summary current modalities presented, highlighting their advantages limitations while exploring potential (EVs) innovative therapeutic tools MASLD. With this aim, review emphasizes in-depth understanding origin EVs pathophysiological alterations these ectosomes exosomes diseases. This also explores key components future management disease. dual utility essentially highlights clinical integration improve treatment. While EV-based still stages development require substantial research increase value before they can be used clinically, application been extensively explored. Moving forward, developing devices leveraging will crucial advancing diagnosis. Thus, literature summarized provides suitable grounds clinicians researchers explore devising treatment strategies

Language: Английский

Single Extracellular VEsicle Nanoscopy DOI Creative Commons
András Saftics, Sarah Abuelreich, Eugenia Romano

et al.

Journal of Extracellular Vesicles, Journal Year: 2023, Volume and Issue: 12(7)

Published: July 1, 2023

Extracellular vesicles (EVs) and their cargo constitute novel biomarkers. EV subpopulations have been defined not only by abundant tetraspanins (e.g., CD9, CD63 CD81) but also specific markers derived from source cells. However, it remains a challenge to robustly isolate characterize subpopulations. Here, we combined affinity isolation with super-resolution imaging comprehensively assess human plasma. Our Single VEsicle Nanoscopy (SEVEN) assay successfully quantified the number of affinity-isolated EVs, size, shape, molecular tetraspanin content, heterogeneity. The detected tetraspanin-enriched EVs positively correlated sample dilution in 64-fold range (for SEC-enriched plasma) 50-fold crude plasma). Importantly, SEVEN as little ∼0.1 μL We further characterized shape content (with corresponding heterogeneities) for CD9-, CD63- CD81-enriched Finally, assessed plasma four pancreatic ductal adenocarcinoma patients resectable disease. Compared healthy plasma, CD9-enriched were smaller while IGF1R-enriched larger, rounder contained more molecules, suggestive unique cancer-enriched subpopulation. This study provides method validation demonstrates that could be advanced into platform characterizing both disease-associated organ-associated

Language: Английский

Citations

45
Harnessing extracellular vesicle heterogeneity for diagnostic and therapeutic applications DOI
Randy P. Carney, Rachel R. Mizenko, Batuhan Turhan Bozkurt

et al.

Nature Nanotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Language: Английский

Citations

19
Extracellular vesicles, from the pathogenesis to the therapy of neurodegenerative diseases DOI Creative Commons
Xiaohuan Xia, Yi Wang, Jialin Zheng

et al.

Translational Neurodegeneration, Journal Year: 2022, Volume and Issue: 11(1)

Published: Dec. 12, 2022

Abstract Extracellular vesicles (EVs) are small bilipid layer-enclosed that can be secreted by all tested types of brain cells. Being a key intercellular communicator, EVs have emerged as contributor to the pathogenesis various neurodegenerative diseases (NDs) including Alzheimer’s disease, Parkinson’s amyotrophic lateral sclerosis, and Huntington’s disease through delivery bioactive cargos within central nervous system (CNS). Importantly, CNS cell-derived purified via immunoprecipitation, EV with altered levels been identified potential biomarkers for diagnosis prognosis NDs. Given essential impact on NDs, pathological considered therapeutic targets effects utilized agents or drug platforms treatment In this review, we focus recent research progress roles released from cells in summarize findings identify CNS-derived diagnose comprehensively discuss promising targets, agents, systems treating together current concerns challenges basic clinical applications regarding

Language: Английский

Citations

50
Current Status of the Diagnosis of Early-Stage Pancreatic Ductal Adenocarcinoma DOI Creative Commons
Kazunori Nakaoka, Eizaburo Ohno, Naoto Kawabe

et al.

Diagnostics, Journal Year: 2023, Volume and Issue: 13(2), P. 215 - 215

Published: Jan. 6, 2023

Pancreatic ductal adenocarcinoma (PDAC) can be treated with surgery, chemotherapy, and radiotherapy. Despite medical progress in each field recent years, it is still insufficient for managing PDAC, at present, the only curative treatment surgery. A typical pancreatic cancer relatively easy to diagnose imaging. However, often not recommended surgical time of diagnosis due metastatic spread beyond pancreas. Even if operable, recurs during postoperative follow-up. In case PDAC a diameter 10 mm or less, 5-year survival rate as good 80% more, best index tumor size. The early detection less than carcinoma situ critical. Here, we provide an overview current status diagnostic imaging features genetic tests accurate early-stage PDAC.

Language: Английский

Citations

25
Extracellular vesicle-mediated ferroptosis, pyroptosis, and necroptosis: potential clinical applications in cancer therapy DOI Creative Commons

Yi-Chi Yang,

Qian Jiang,

Ke-Ping Yang

et al.

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: Jan. 12, 2024

Abstract Extracellular vesicles (EVs) have gained increasing recognition as significant regulators of intercellular communication in various physiological and pathological processes. These play a pivotal role cancer progression by facilitating the transfer diverse cargoes, including lipids, proteins, nucleic acids. Regulated cell death (RCD), orderly autonomous cells, is controlled variety biomacromolecules and, turn, influences biological processes progression. Recent studies demonstrated that EV cargoes regulate oncogenes tumor suppressors to mediate different nonapoptotic forms RCD, notably ferroptosis, pyroptosis, necroptosis. Nevertheless, comprehensive exploration EV-mediated RCD context has not been performed. This review summarizes progress regarding functions underlying mechanisms EVs mediating delivery Additionally, delves into potential clinical applications its significance areas diagnosis therapy.

Language: Английский

Citations

13
Circulating tumor extracellular vesicles to monitor metastatic prostate cancer genomics and transcriptomic evolution DOI Creative Commons
Irene Casanova‐Salas, Daniel Aguilar,

Sarai Córdoba-Terreros

et al.

Cancer Cell, Journal Year: 2024, Volume and Issue: 42(7), P. 1301 - 1312.e7

Published: July 1, 2024

Extracellular vesicles (EVs) secreted by tumors are abundant in plasma, but their potential for interrogating the molecular features of through multi-omic profiling remains widely unexplored. Genomic and transcriptomic circulating EV-DNA EV-RNA isolated from vitro vivo models metastatic prostate cancer (mPC) reveal a high contribution tumor material to EV-loaded DNA/RNA, validating findings two cohorts longitudinal plasma samples collected patients during androgen receptor signaling inhibitor (ARSI) or taxane-based therapy. genomic recapitulate matched-patient biopsies DNA (ctDNA) associate with clinical progression. We develop novel approach enable (RExCuE). report how transcriptome EVs is enriched tumor-associated transcripts, captures certain patient features, reflects on-therapy adaptation changes. Altogether, we show that EV enables mPC liquid biopsy.

Language: Английский

Citations

13
Harnessing exosomes as cancer biomarkers in clinical oncology DOI Creative Commons
Subhrojyoti Ghosh, Ramya Lakshmi Rajendran, Atharva Anand Mahajan

et al.

Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)

Published: Aug. 7, 2024

Exosomes are extracellular vesicles well known for facilitating cell-to-cell communication by distributing essential macromolecules like proteins, DNA, mRNA, lipids, and miRNA. These abundant in fluids distributed throughout the body, including urine, blood, saliva, even bile. They important diagnostic tools breast, lung, gastrointestinal cancers, etc. However, their application as cancer biomarkers has not yet been implemented most parts of world. In this review, we discuss how OMICs profiling exosomes can be practiced substituting traditional imaging or biopsy methods detection. Previous extensive used screening were expensive, mostly invasive, could easily provide early detection various types cancer. Exosomal utilized routine simply collecting body from individual. We anticipate that use will brought to light success clinical trials investigating potential enhance treatment upcoming years.

Language: Английский

Citations

13
Proteomic profiling of mesenchymal stem cell‐derived extracellular vesicles: Impact of isolation methods on protein cargo DOI Creative Commons
Morteza Abyadeh, Shahab Mirshahvaladi, Sara Assar Kashani

et al.

Journal of Extracellular Biology, Journal Year: 2024, Volume and Issue: 3(6)

Published: June 1, 2024

Abstract Extracellular vesicles (EVs) are nanosized with a lipid bilayer that secreted by cells and play critical role in cell‐to‐cell communication. Despite the promising reports regarding their diagnostic therapeutic potential, utilization of EVs clinical setting is limited due to insufficient information about cargo lack standardization isolation analysis methods. Considering protein cargos as key contributors potency, we conducted tandem mass tag (TMT) quantitative proteomics three subpopulations mesenchymal stem cell (MSC)‐derived obtained through different techniques: ultracentrifugation (UC), high‐speed centrifugation (HS), on sucrose cushion (SU). Subsequently, checked EV marker expression, size distribution, morphological characterization, followed bioinformatic analysis. The proteome results revealed these exhibit distinct molecular functional characteristics. choice method impacts isolated isolating EVs. Specifically, (HS) exhibited higher abundance ribosomal mitochondrial proteins. Functional apoptosis assays comparing mitochondria methods HS‐EVs, but not other EVs, induced early cancer cells. On hand, using (SU) (UC) demonstrated proteins primarily involved immune response, cell‐cell interactions extracellular matrix interactions. Our analyses unveil notable disparities associated biological functions among subpopulations, underscoring importance meticulously selecting resultant based intended application.

Language: Английский

Citations

12
Single extracellular vesicle surface protein‐based blood assay identifies potential biomarkers for detection and screening of five cancers DOI Creative Commons

Yuxin Min,

Wenjiang Deng, Huangbo Yuan

et al.

Molecular Oncology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 9, 2024

Extracellular vesicles (EVs) and EV proteins are promising biomarkers for cancer liquid biopsy. Herein, we designed a case–control study involving 100 controls patients with esophageal, stomach, colorectal, liver, or lung to identify common type‐specific of plasma‐derived surface the five cancers. were profiled using sequencing‐based proximity barcoding assay. In this study, differentially expressed (DEPs) eight protein combinations (DEPCs) showed performance (area under curve, AUC > 0.900) in pan‐cancer identification [e.g., TENM2 (AUC = 0.982), CD36 0.974), CD36‐ITGA1 0.971)]. Our classification model could properly discriminate between DEPs 0.981) DEPCs 0.965). When distinguishing one from other four, accuracy (85–92%) was higher than that (78–84%). We validated an additional 14 controls, achieved value 0.786 0.622 DEPCs, highlighting necessity recruit larger cohort further validation. clustering EVs into subpopulations, detected cluster‐specific highly immune‐related tissues. context colorectal cancer, identified heterogeneous clusters enriched patients, correlating tumor initiation progression. These findings provide epidemiological molecular evidence clinical application prediction, while also illuminating their functional roles physiopathology.

Language: Английский

Citations

11
Advances in magnetic affinity-based isolation/detection of exosomes for robust diagnostics DOI Creative Commons

Veronika Solovicová,

Anna Ďatková,

Tomáš Bertók

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(4)

Published: March 5, 2025

The review article provides a short introduction to exosomes with the focus use as disease markers itself (i.e. their concentration or presence of some specific receptors) source biomarkers such proteins and metabolites. In detail, we are discussing various methods exosome isolation main paper is on affinity capture exosomes, since them can be applied sub-populations produced by organs. comprehensive overview magnetic (bio)affinity detection exosomal cargo using different ligands antibodies, DNA aptamers, peptides, glycan-based recognition, transferrin-based approaches, based recognition phospholipids other approaches including electrostatic interactions. in detail key analytical clinical parameters form an extensive table summarising outcomes published last two years (2023-2024). Finally, also conclusions sections pros cons for and/or determination content.

Language: Английский

Citations

1