Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing DOI Creative Commons
Xueying Han, Ziniu Deng, Huiyun Liu

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 889 - 889

Published: March 12, 2025

Recent years have witnessed remarkable progress in plant biology, driven largely by the rapid evolution of CRISPR/Cas-based genome editing (GE) technologies. These tools, including versatile CRISPR/Cas systems and their derivatives, such as base editors prime editors, significantly enhanced universality, efficiency, convenience functional genomics, genetics, molecular breeding. However, traditional genetic transformation methods are essential for obtaining GE plants. depend on tissue culture procedures, which time-consuming, labor-intensive, genotype-dependent, challenging to regenerate. Here, we systematically outline current advancements simplifying GE, focusing optimization process through developmental regulators, development planta methods, establishment nanomaterial- viral vector-based delivery platforms. We also discuss critical challenges future directions achieving genotype-independent, culture-free GE.

Language: Английский

Targeted genome-modification tools and their advanced applications in crop breeding DOI
Boshu Li, Chao Sun, Jiayang Li

et al.

Nature Reviews Genetics, Journal Year: 2024, Volume and Issue: 25(9), P. 603 - 622

Published: April 24, 2024

Language: Английский

Citations

55
Plant virology in the 21st century in China: Recent advances and future directions DOI Creative Commons
Jianguo Wu, Yongliang Zhang, Fangfang Li

et al.

Journal of Integrative Plant Biology, Journal Year: 2023, Volume and Issue: 66(3), P. 579 - 622

Published: Nov. 4, 2023

Plant viruses are a group of intracellular pathogens that persistently threaten global food security. Significant advances in plant virology have been achieved by Chinese scientists over the last 20 years, including basic research and technologies for preventing controlling viral diseases. Here, we review these milestones advances, identification new crop-infecting viruses, dissection pathogenic mechanisms multiple examination multilayered interactions among their host plants, virus-transmitting arthropod vectors, in-depth interrogation plant-encoded resistance susceptibility determinants. Notably, various virus-based vectors also successfully developed gene function studies target expression plants. We recommend future China.

Language: Английский

Citations

50
Plant regeneration in the new era: from molecular mechanisms to biotechnology applications DOI
Chunli Chen, Yuxin Hu, Momoko Ikeuchi

et al.

Science China Life Sciences, Journal Year: 2024, Volume and Issue: 67(7), P. 1338 - 1367

Published: May 31, 2024

Language: Английский

Citations

18
The type V effectors for CRISPR/Cas-mediated genome engineering in plants DOI
Ruixiang Zhang, Nan Chai, Taoli Liu

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 74, P. 108382 - 108382

Published: May 25, 2024

Language: Английский

Citations

14
CRISPR/Cas genome editing in plants: mechanisms, applications, and overcoming bottlenecks DOI
Delight Hwarari,

Yasmina Radani,

Yongchao Ke

et al.

Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(2)

Published: March 5, 2024

Language: Английский

Citations

13
Recent advances of CRISPR-based genome editing for enhancing staple crops DOI Creative Commons
Feng Chen, Chen D. Lu, Yan Zhao

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 23, 2024

An increasing population, climate change, and diminishing natural resources present severe threats to global food security, with traditional breeding genetic engineering methods often falling short in addressing these rapidly evolving challenges. CRISPR/Cas systems have emerged as revolutionary tools for precise modifications crops, offering significant advancements resilience, yield, nutritional value, particularly staple crops like rice maize. This review highlights the transformative potential of technology, emphasizing recent innovations such prime base editing, development novel CRISPR-associated proteins, which significantly improved specificity, efficiency, scope genome editing agriculture. These enable targeted that enhance tolerance abiotic stresses well biotic stresses. Additionally, plays a crucial role improving crop yield quality by enhancing photosynthetic nutrient uptake, resistance lodging, while also taste, texture, shelf life, content through biofortification. Despite challenges off-target effects, need more efficient delivery methods, ethical regulatory concerns, underscores importance security sustainability It calls continued research integration CRISPR other emerging technologies nanotechnology, synthetic biology, machine learning fully realize its developing resilient, productive, sustainable agricultural systems.

Language: Английский

Citations

12
Enhancing virus-mediated genome editing for cultivated tomato through low temperature DOI

Ga Hui Kang,

Young Jin Ko, Je Min Lee

et al.

Plant Cell Reports, Journal Year: 2025, Volume and Issue: 44(1)

Published: Jan. 1, 2025

Language: Английский

Citations

1
CRISPR–Cas applications in agriculture and plant research DOI
Aytug Tuncel, Changtian Pan,

Joshua S. Clem

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Language: Английский

Citations

1
Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration DOI Creative Commons
Zuzana Kocsisova, Viktoriya Coneva

Frontiers in Genome Editing, Journal Year: 2023, Volume and Issue: 5

Published: July 20, 2023

Increased understanding of plant genetics and the development powerful easier-to-use gene editing tools over past century have revolutionized humankind's ability to deliver precise genotypes in crops. Plant transformation techniques are well developed for making transgenic varieties certain crops model organisms, yet reagent delivery regeneration remain key bottlenecks applying technology most Typical protocols produce transgenic, genetically modified (GM) rely on transgenes, chemical selection, tissue culture. make edited (GE) also use even though these may be undesirable final crop product. In some crops, transgenes routinely segregated away during meiosis by performing crosses, thus only a minor concern. other particularly those propagated vegetatively, complex hybrids, or with long generation times, such crosses impractical impossible. This review highlights diverse strategies CRISPR/Cas reagents regenerable cells recover plants without unwanted integration transgenes. Some examples include delivering DNA-free as ribonucleoproteins mRNA, relying expression from non-integrated DNA, using novel mechanisms viruses nanoparticles, unconventional selection methods avoid and/or avoiding culture altogether. These advancing rapidly already enabling scientists precision CRISPR tools.

Language: Английский

Citations

19
Generation of transgene-free canker-resistant Citrus sinensis cv. Hamlin in the T0 generation through Cas12a/CBE co-editing DOI Creative Commons

Hongge Jia,

Ahmad A. Omar, Jin Xu

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: March 26, 2024

Citrus canker disease affects citrus production. This is caused by Xanthomonas citri subsp. (Xcc). Previous studies confirmed that during Xcc infection, PthA4, a transcriptional activator like effector (TALE), translocated from the pathogen to host plant cells. PthA4 binds binding elements (EBEs) in promoter region of susceptibility gene LOB1 (EBE -LOBP) activate its expression and subsequently cause symptoms. Previously, Cas12a/CBE co-editing method was employed disrupt EBE -LOBP pummelo, which highly homozygous. However, most commercial cultivars are heterozygous hybrids more difficult generate homozygous/biallelic mutants. Here, we edit Hamlin ( sinensis ), hybrid cultivar grown worldwide. Binary vector GFP-p1380N-ttLbCas12a:LOBP1-mPBE:ALS2:ALS1 constructed shown be functional via Xcc-facilitated agroinfiltration leaves. construct allows selection transgene-free regenerants GFP, edits ALS chlorsulfuron-resistant as marker for genome editing resulting transient T-DNA nCas9-mPBE:ALS2:ALS1, gene(s) interest (i.e., this study) through ttLbCas12a, thus creating citrus. Totally, 77 plantlets were produced. Among them, 8 transgenic plants (#Ham GFP 1 - #Ham 8), 4 NoGFP 4), rest wild type. plantlets, three lines 1, 2 3) contained biallelic mutations pthA4 , one line 4) had homozygous . We achieved 5.2% mutation efficiency –LOBP C. cv. Hamlin, compared 1.9% pummelo previous study. Importantly, four 3 survived resistant against canker. Taken together, has been successfully used canker‐resistant T0 generation biallelic/homozygous LOB1.

Language: Английский

Citations

7