Cereal Research Communications, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 28, 2024
Language: Английский
Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
Summary Improving cold tolerance at the flowering stage (CTF) in rice is crucial for minimising yield loss, making identification and application of cold‐tolerant genes QTLs imperative effective molecular breeding. The long lead time, dependence on treatment conditions, inherent complexity trait make studying genetic basis CTF challenging. To date, fine‐mapping or cloning specific to has not yet been achieved. In this study, single segment substitution lines (SSSLs) were constructed using HJX74 as recipient IR58025B, known good CTF, donor. This approach led two QTLs, qCTF3 qCTF6 , rice. promising breeding potential. Further, we identified causal gene CTF1 underlying through map‐based . which encodes a conserved putative protein, SNPs within its coding sequence that influence Additionally, variations promoter also contributes CTF. Thirteen variant sites four SSSLs are consistent with IR58025B. Moreover, analysed 307 accessions characterise haplotypes based 13 variation sites, identifying five distinct haplotypes. selection evolutionary analysis indicate haplotype newly generated mutation undergone japonica during domestication. study only provides novel favourable but highlights potential advancing
Language: Английский
Citations
2
New Phytologist, Journal Year: 2024, Volume and Issue: 241(5), P. 2059 - 2074
Published: Jan. 10, 2024
Summary Thermo‐sensitive genic male sterile (TGMS) lines are the core of two‐line hybrid rice ( Oryza sativa ). However, elevated or unstable critical sterility‐inducing temperatures (CSITs) TGMS bottlenecks that restrict development rice. genes and molecular mechanisms controlling CSIT remain unknown. Here, we report CRITICAL STERILITY‐INDUCING TEMPERATURE 2 CSIT2 ) encodes a really interesting new gene (RING) type E3 ligase, thermo‐sensitive sterility 5 tms5 )‐based through ribosome‐associated protein quality control (RQC). binds to large small ribosomal subunits ubiquitinates 80S ribosomes for dissociation, may also ubiquitinate misfolded proteins degradation. Mutation inhibits possible damage ubiquitin system translation, which allows more such as catalases accumulate anther abnormal accumulation reactive oxygen species (ROS) premature programmed cell death (PCD) in anthers, partly rescuing raised ‐based lines. These findings reveal mechanism provide strategy solving CSITs
Language: Английский
Citations
15
Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: 22(8), P. 2267 - 2281
Published: March 25, 2024
Summary Inter‐subspecific indica – japonica hybrid rice ( Oryza sativa ) has the potential for increased yields over traditional intra‐subspecies rice, but limited yield of F 1 seed production (FHSP) hinders development breeding. Diurnal flower‐opening time (DFOT) divergence between and been a major contributing factor to this issue, few DFOT genes have cloned. Here, we found that manipulating expression jasmonate (JA) pathway can effectively modulate improve FHSP in rice. Treating cultivar Zhonghua 11 (ZH11) with methyl (MeJA) substantially advanced DFOT. Furthermore, overexpressing JA biosynthesis gene OPDA REDUCTASE 7 OsOPR7 knocking out inactivation CHILLING TOLERANCE OsHAN1 ZH11 by 1‐ 2‐h respectively; knockout signal suppressor JASMONATE ZIM‐DOMAIN PROTEIN OsJAZ7 OsJAZ9 resulted 50‐min 1.5‐h earlier respectively. The using male‐sterile lines GAZS manipulated were significantly higher than wildtype. Transcriptome analysis, cytological observations, measurements elastic modulus determination cell wall components indicated could affect loosening lodicule walls regulating their composition through controlling sugar metabolism, which turn influences This research vital implications breeding cultivars early facilitate
Language: Английский
Citations
15
Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: 22(7), P. 2020 - 2032
Published: Feb. 29, 2024
Summary P/TGMS (Photo/thermo‐sensitive genic male sterile) lines are crucial resources for two‐line hybrid rice breeding. Previous studies revealed that slow development is a general mechanism sterility–fertility conversion of in Arabidopsis. However, the difference genes between and Arabidopsis suggests presence distinct rice. In this study, we isolated novel line, ostms19 , which shows sterility under high‐temperature conditions fertility low‐temperature conditions. OsTMS19 encodes pentatricopeptide repeat (PPR) protein essential pollen formation, point mutation GTA(Val) to GCA(Ala) leads phenotype. It highly expressed tapetum localized mitochondria. Under high temperature or long‐day photoperiod conditions, excessive ROS accumulation anthers during mitosis disrupts gene expression intine causing sterility. Conversely, low short‐day can be effectively scavenged anthers, resulting restoration. This indicates homeostasis critical conversion. relationship has also been observed other tested lines. Therefore, propose
Language: Английский
Citations
14
Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(6)
Published: Feb. 6, 2025
The two-line hybrid rice system, a cutting-edge breeding technology, has greatly boosted global food security. In thermo-sensitive genic male sterile (TGMS) lines, the critical sterility-inducing temperature (CSIT; at which TGMS lines change from fertile to complete sterile) acts as key threshold. We recently uncovered that sterility 5 ( tms5 ), locus presenting in over 95% of leads overaccumulation 2′,3′-cyclic phosphate (cP)-ΔCCA-tRNAs and deficiency mature tRNAs, underlies molecular mechanism -mediated TGMS. However, there are few reports on regulatory controlling CSIT. Here, we identified suppressor , an amino acid substitution (T552I) Rqc2 (ribosome-associated quality control 2), increases CSIT through its C-terminal alanine threonine modification (CATylation) activity. This alters tRNA selectivity, leading recruitment different tRNAs A-site ribosome CATylation rate by OsRqc2 during ribosome-associated (RQC), process rescues stalled ribosomes degrades abnormal nascent chains translational elongation. Further, mutation restores levels tRNA-Ser/Ile increase lines. Our findings reveal origin overaccumulated cP-ΔCCA-tRNAs further deepening our understanding network governing containing tms5.
Language: Английский
Citations
1
Chinese Science Bulletin (Chinese Version), Journal Year: 2025, Volume and Issue: 70(7), P. 807 - 815
Published: Feb. 8, 2025
Citations
1
The Plant Journal, Journal Year: 2024, Volume and Issue: 119(6), P. 2585 - 2598
Published: July 7, 2024
Diurnal flower-opening time (DFOT), the of spikelet opening during day, is an important trait for hybrid rice (Oryza sativa L.) seed production. Hybrids between indica and japonica varieties have strong heterosis, but parental lines usually different, nonoverlapping DFOTs. This reduces success production in crosses subspecies, thus hindering utilization inter-subspecies heterosis. However, little known about molecular mechanisms regulating DFOT rice. Here, we obtained with a 1.5 h earlier than wild type by overexpressing OsMYC2, gene encoding key transcription factor jasmonate (JA) signaling pathway. OsMYC2 activated JA directly regulates genes related to biosynthesis cell wall metabolism. Overexpressing led significantly increased contents decreased cellulose hemicellulose lodicule cells, as well softening walls. may facilitate swelling lodicules, resulting early diurnal flower-opening. These results suggest that OsMYC2-JA feedback loop via remodeling. findings shed light on understanding regulatory mechanism plants, which should promote development suitable breeding.
Language: Английский
Citations
8
Cell Research, Journal Year: 2024, Volume and Issue: 34(11), P. 763 - 775
Published: Sept. 9, 2024
Language: Английский
Citations
6
The Plant Journal, Journal Year: 2024, Volume and Issue: 118(2), P. 506 - 518
Published: Jan. 3, 2024
Thermosensitive genic female sterility (TGFS) is a promising property to be utilized for hybrid breeding. Here, we identified rice TGFS line, tfs2, through an ethyl methyl sulfone (EMS) mutagenesis strategy. This line showed under high temperature and became fertile low temperature. Few seeds were produced when the tfs2 stigma was pollinated, indicating that sterile. Gene cloning genetic complementation point mutation from leucine phenylalanine in HEI10 (HEI10
Language: Английский
Citations
4
Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 28, 2025
ABSTRACT Reactive oxygen species (ROS) plays critical roles in modulating plant growth and stress response its homeostasis is fine tuned using multiple peroxidases. H 2 O , a major kind of ROS, removed rapidly directly three catalases, CAT1, CAT2, CAT3, Arabidopsis. Although the activity regulations catalases have been well studied, their degradation pathway less clear. Here, we report that CAT2 CAT3 protein abundance was partially controlled 26S proteasome. To further identify candidate proteins modulate stability performed yeast‐two‐hybrid screening recovered several clones encoding with RING vWA domains, CIRP1 ( C AT2 I nteracting R ING P rotein 1 ). Drought oxidative downregulated transcripts. harbored E3 ubiquitination accelerated by direct interaction ubiquitination. The cirp1 mutants exhibited stronger drought tolerance, which opposite to cat2 cat3 mutants. Genetic analysis revealed acts upstream negatively regulate tolerance. increased tolerance due enhanced catalase (CAT) activities alleviated ROS levels. Our data CIRP1–CAT2/CAT3 module vital role alleviating levels balancing responses
Language: Английский
Citations
0