Bioactive Materials, Journal Year: 2025, Volume and Issue: 50, P. 95 - 115
Published: April 7, 2025
Language: Английский
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(19)
Published: March 1, 2024
Abstract Exosomes, nanoparticles secreted by various cells, composed of a bilayer lipid membrane, and containing bioactive substances such as proteins, nucleic acids, metabolites, etc., have been intensively investigated in tissue engineering owing to their high biocompatibility versatile biofunction. However, there is still lack high‐quality review on bone defect regeneration potentiated exosomes. In this review, the biogenesis isolation methods exosomes are first introduced. More importantly, engineered current state knowledge discussed review. Afterward, biomaterial carriers mechanisms repair elucidated compelling evidence presented. Thus, future perspectives concerns revealed help devise advanced modalities based overcome challenges regeneration. It totally believed will attract special attention from clinicians provide promising ideas for works.
Language: Английский
Citations
8
Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 346, P. 122614 - 122614
Published: Aug. 14, 2024
Language: Английский
Citations
8
Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)
Published: Jan. 25, 2024
Abstract Accumulating evidence indicates that exosomes help to regulate bone homeostasis. The roles of bone-derived have been well-described; however, recent studies shown some non-bone-derived better targeting ability than and their performance as a drug delivery vehicle for regulating homeostasis may be exosomes, the sources are more extensive can thus clinical needs. Here, we sort describe composition biogenesis. Their specific mechanisms in bone-related diseases also discussed. Furthermore, reveal obstacles current research future challenges practical application provide potential strategies effective regulation treatment diseases.
Language: Английский
Citations
7
ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown
Published: April 25, 2024
Despite considerable advances in artificial bone tissues, the absence of neural network reconstruction their design often leads to delayed or ineffective healing. Hence, we propose a multilayer hierarchical lithium (Li)-doped titanium dioxide structure, constructed through microarc oxidation combined with alkaline heat treatment. This structure can induce sustained release Li ions, mimicking environment neurogenic osteogenesis characterized by high brain-derived neurotrophic factor (BDNF) expression. During vitro experiments, enhanced differentiation Schwann cells (SCs) and growth human umbilical vein endothelial (HUVECs) mouse embryo osteoblast progenitor (MC3T3-E1). Additionally, coculture system, SC-conditioned media markedly increased phosphatase expression formation calcium nodules, demonstrating excellent potential material for nerve-induced regeneration. In an vivo experiment based on rat distal femoral lesion model, substantially healing increasing density tissue around implant. conclusion, this study elucidates neuromodulatory pathways involved regeneration, providing promising method addressing deformities.
Language: Английский
Citations
7
Materials Today Bio, Journal Year: 2023, Volume and Issue: 22, P. 100728 - 100728
Published: July 19, 2023
The development of surface modification techniques has brought about a major paradigm shift in the clinical applications bone tissue regeneration. Biofabrication strategies enable creation scaffolds with specific microstructural environments and biological components. Lithium (Li) been reported to exhibit anti-inflammatory, osteogenic, chondrogenic properties by promoting several intracellular signaling pathways. Currently, research focuses on fabricating simultaneous dual bioactivities enhance osteochondral In this study, we modified calcium silicate (CS) Li using simple immersion technique evaluated their capabilities for results showed that ions could be easily coated onto surfaces CS without affecting itself. Furthermore, modifications did not affect printing CS, porous fabricated via extrusion. Moreover, presence improved roughness hydrophilicity, thus leading enhanced secretion osteochondral-related regeneration factors, such as alkaline phosphatase (ALP), sialoprotein (BSP), collagen II (Col II) proteins. Subsequent vivo studies, including histological micro-CT analyses, confirmed Li-modified promoted transcriptome analysis suggested osteochondrogenic our were influenced paracrine exosomes. We hope study will inspire further
Language: Английский
Citations
15
Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)
Published: Aug. 24, 2023
Abstract The regeneration of weight-bearing bone defects and critical-sized cartilage remains a significant challenge. A wide range nano-biomaterials are available for the treatment bone/cartilage defects. However, their poor compatibility biodegradability pose challenges to practical applications these nano-based biomaterials. Natural biomaterials inspired by cell units (e.g., nucleic acids proteins), have gained increasing attention in recent decades due versatile functionality, compatibility, biodegradability, great potential modification, combination, hybridization. In field regeneration, natural presented an unparalleled role providing optimal cues microenvironments growth differentiation. this review, we systematically summarize building blocks unit used as including acids, proteins, carbohydrates, lipids, membranes. addition, opportunities future use discussed.
Language: Английский
Citations
11
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(27)
Published: July 19, 2024
Abstract An interbody fusion cage (Cage) is crucial in spinal decompression and procedures for restoring normal vertebral curvature rebuilding stability. Currently, these Cages suffer from issues related to mismatched elastic modulus insufficient bone integration capability. Therefore, a gel‐casting technique utilized fabricate biomimetic porous titanium alloy material Ti6Al4V powder. The compared with polyetheretherketone (PEEK) 3D‐printed materials their respective Cages. Systematic validation performed through mechanical testing, vitro cell, vivo rabbit defect implantation, ovine anterior cervical discectomy experiments evaluate the biological performance of materials. Although all three demonstrate good biocompatibility osseointegration properties, Ti6Al4V, its excellent properties structure closely resembling trabecular tissue, exhibited superior ingrowth performance. Compared PEEK Cages, Cage outperforms terms intervertebral performance, achieving without need grafting, thereby enhancing vertebra This offers cost‐effectiveness, presenting significant potential clinical applications surgery.
Language: Английский
Citations
4
Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2023, Volume and Issue: 15(4)
Published: April 10, 2023
Abstract Osteoclasts are the cells responsible for bone resorption process during remodeling. In a healthy situation, this results from an equilibrium between new matrix formation by osteoblast and osteoclast. Osteoporosis (OP) is systemic disease characterized decreased mass density alterations in microarchitecture, increasing fracture predisposition. Despite variety of available therapies OP management there growing gap its treatment associated to low patients' adherence owing concerns related with long‐term efficacy or safety. This makes development safe treatments necessary. Among newly developed strategies, use synthetic natural nanoparticles modulate osteoclasts differentiation, activity, apoptosis crosstalk osteoblasts have arisen. Synthetic exert their therapeutic effect either loading antiresorptive drugs including molecules gene regulation. Moreover, control over can be improved targeting extracellular osteoclast membranes. Furthermore, nanoparticles, also known as vesicles, been identified play key role homeostasis. Consequently, these systems widely studied under variable environments. Additionally, ability bioengineer vesicles has allowed obtain biomimetic desirable characteristics drug carriers osteoclasts. The analyzed information reveals possibility modulating different mechanisms through decreasing resorption. These findings suggest that controlling activity using potential improve osteoporosis management. article categorized under: Implantable Materials Surgical Technologies > Nanomaterials Implants Nanotechnology Tissue Repair Replacement Approaches Biology Nanoscale Systems
Language: Английский
Citations
11
BMEMat, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 18, 2025
Abstract Bone tissue is richly innervated, and the influence of nervous system on physiological pathological status bone has emerged as a significant research focus. The recent discovery skeletal interoceptive circuits further emphasizes crucial role central in control homeostasis. Skeletal interoception represents one most intricate mechanisms human body for maintaining homeostasis, it involves orchestrated efforts skeletal, nervous, immune, endocrine systems. In this review, we comprehensively introduce three primary components circuitry, including ascending pathways that perceive convey signals to system, neural process interpret these signals, descending mediate regulatory effects tissue. We also discuss how innovative therapeutic strategies can be developed modulate homeostasis by leveraging updated findings circuitry. anticipate application knowledge will lead paradigm shift field orthopaedics biomaterials.
Language: Английский
Citations
0
Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Severe periodontitis frequently leads to irreversible degradation of alveolar bone. Periodontal regeneration techniques hold immense potential in reconstructing bone post periodontal diseases. Schwann cells (SCs) play a critical role supporting, maintaining, and regenerating tissues, with SC-derived exosomes (SC-EXO) exhibiting cell homing tissue repair capabilities. Nevertheless, the specific SC-EXO remains unknown. To address issue, we treated human ligament (hPDLCs) observed notable increase proliferation, osteogenic neurogenic differentiation. Moreover, stimulated expression angiogenic factors vascular endothelial cells. In rat model defects, facilitated recruitment endogenous cells, regulated neural neogenesis, accelerated regeneration. This study successfully prepared SC-EXO, which effectively promotes by regulating healing microenvironment, potentially offering valuable strategy for engineering.
Language: Английский
Citations
0