A single-cell nanocoating of probiotics for enhanced amelioration of antibiotic-associated diarrhea

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: April 19, 2022

The gut microbiota represents a large community of microorganisms that play an important role in immune regulation and maintenance homeostasis. Living bacteria receive increasing interest as potential therapeutics for disorders, because they inhibit the colonization pathogens positively regulate composition gut. However, these treatments are often accompanied by antibiotic administration targeting pathogens. In cases, efficacy therapeutic is compromised their susceptibility to antibiotics. Here, we demonstrate single-cell coating composed tannic acids ferric ions, referred 'nanoarmor', can protect from action nanoarmor protects both Gram-positive Gram-negative against six clinically relevant multiple interactions between molecules allow antibiotics be effectively absorbed onto nanoarmor. Armored probiotics have shown ability colonize inside gastrointestinal tracts levofloxacin-treated rats, which significantly reduced antibiotic-associated diarrhea (AAD) resulting levofloxacin-treatment improved some pre-inflammatory symptoms caused AAD. This strategy robust platform enhance potency patients receiving avoid negative effects tract.

Language: Английский

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Mucoadhesive probiotic backpacks with ROS nanoscavengers enhance the bacteriotherapy for inflammatory bowel diseases

Science Advances, Journal Year: 2022, Volume and Issue: 8(45)

Published: Nov. 11, 2022

Inflammatory bowel diseases (IBDs) are often associated with elevated levels of reactive oxygen species (ROS) and highly dysregulated gut microbiota. In this study, we synthesized a polymer hyaluronic acid–poly(propylene sulfide) (HA-PPS) developed ROS-scavenging nanoparticles (HPN) that could effectively scavenge ROS. To achieve colon tissue targeting effects, the HPN were conjugated to surface modified probiotic Escherichia coli Nissle 1917 (EcN). enhance bacteriotherapy EcN, encapsulated EcN cells poly-norepinephrine (NE) layer can protect against environmental assaults improve viability in oral delivery prolong retention time intestine due its strong mucoadhesive capability. dextran sulfate sodium–induced mouse colitis models, HPN-NE-EcN showed substantially enhanced prophylactic therapeutic efficacy. Furthermore, abundance diversity microbiota increased after treatment HPN-NE-EcN, contributing alleviation IBDs.

Language: Английский

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Nanoencapsulation for Probiotic Delivery

ACS Nano, Journal Year: 2021, Volume and Issue: 15(12), P. 18653 - 18660

Published: Dec. 3, 2021

Gut microbiota dynamically participate in diverse physiological activities with direct impact on the host's health. A range of factors associated highly complex intestinal flora ecosystem poses challenges regulating homeostasis microbiota. The consumption live probiotic bacteria, principle, can address these and confer health benefits. In this context, one major problems is ensuring survival cells when faced physical chemical assaults during their intake subsequent gastrointestinal passage to gut. Advances field have focused improving conventional encapsulation techniques microscale achieve high cell viability, gastric temperature resistance, longer shelf lives. However, microencapsulation approaches are known limitations possible difficulties clinical translation. Perspective, we present a brief overview current progress different methods highlight contemporary emerging single-cell strategies using nanocoatings for individual cells. Finally, discuss relative advantages various nanoencapsulation future trend toward developing coated probiotics advanced features

Language: Английский

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Novel nano-encapsulated probiotic agents: Encapsulate materials, delivery, and encapsulation systems

Journal of Controlled Release, Journal Year: 2022, Volume and Issue: 349, P. 184 - 205

Published: July 8, 2022

Language: Английский

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Engineered Living Materials For Sustainability

Chemical Reviews, Journal Year: 2022, Volume and Issue: 123(5), P. 2349 - 2419

Published: Dec. 13, 2022

Recent advances in synthetic biology and materials science have given rise to a new form of materials, namely engineered living (ELMs), which are composed matter or cell communities embedded self-regenerating matrices their own artificial scaffolds. Like natural such as bone, wood, skin, ELMs, possess the functional capabilities organisms, can grow, self-organize, self-repair when needed. They also spontaneously perform programmed biological functions upon sensing external cues. Currently, ELMs show promise for green energy production, bioremediation, disease treatment, fabricating advanced smart materials. This review first introduces dynamic features systems potential developing novel We then summarize recent research progress on emerging design strategies from both perspectives. Finally, we discuss positive impacts promoting sustainability key future directions.

Language: Английский

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Decorated bacteria and the application in drug delivery

Advanced Drug Delivery Reviews, Journal Year: 2022, Volume and Issue: 188, P. 114443 - 114443

Published: July 8, 2022

Language: Английский

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Precise oral delivery systems for probiotics: A review

Journal of Controlled Release, Journal Year: 2022, Volume and Issue: 352, P. 371 - 384

Published: Oct. 30, 2022

Language: Английский

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Cell‐Mediated Biointerfacial Phenolic Assembly for Probiotic Nano Encapsulation

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 32(26)

Published: March 22, 2022

Abstract The use of cell‐mediated chemistry is an emerging strategy that exploits the metabolic processes living cells to develop biomimetic materials with advanced functionalities and enhanced biocompatibility. Here, a concept catalytic process for forming protective nano‐shells on individual probiotic demonstrated. This leveraged by cell environment induce oxidative polymerization phenolic compounds, simultaneously these polymers assemble form nano‐coatings around surfaces. detailed analysis reveals oxidation triggered essential nutrient (manganese) cells, which significantly increases rate compounds. coatings, encapsulating each in nanometre scale, demonstrate excellent biocompatibility biodegradability. Additionally, situ encapsulated display improved gastric tolerance up ≈1.4 times higher than native adhesion as high ≈1.6 onto model intestinal epithelial cells. Finally, coated exhibit antioxidant activity feature. Overall, this method provides unique approach improve delivery using machinery engineer nanocoatings benefits new functionalities.

Language: Английский

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Orally administration of cerium oxide nanozyme for computed tomography imaging and anti-inflammatory/anti-fibrotic therapy of inflammatory bowel disease

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Jan. 19, 2023

Inflammatory bowel disease (IBD) is a chronic nonspecific with unknown etiology. Currently, the anti-inflammatory therapeutic approaches have achieved certain extent of effects in terms inflammation alleviation. Still, final pathological outcome intestinal fibrosis has not been effectively improved yet.In this study, dextran-coated cerium oxide (D-CeO2) nanozyme superoxide dismutase (SOD) and catalase (CAT) activities was synthesized by chemical precipitation. Our results showed that D-CeO2 could efficiently scavenge reactive species (ROS) as well downregulate pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, iNOS) to protect cells from H2O2-induced oxidative damage. Moreover, suppress expression fibrosis-related gene levels, such α-SMA, Collagen 1/3, demonstrating anti-fibrotic effect. In both TBNS- DSS-induced colitis models, oral administration chitosan/alginate hydrogel alleviated inflammation, reduced colonic damage scavenging ROS, decreased inflammatory factor levels. Notably, our findings also suggested cytokine predicting contribution alleviating fibrosis. Meanwhile, be employed CT contrast agent for noninvasive gastrointestinal tract (GIT) imaging.We introduced novel approach computed tomography (CT)-guided therapy management IBD. Collectively, without appreciable systemic toxicity, held promise integrated applications diagnosis therapy, pioneering exploration nanozymes ROS capacity treatment

Language: Английский

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Biointerface mineralization generates ultraresistant gut microbes as oral biotherapeutics

Science Advances, Journal Year: 2023, Volume and Issue: 9(11)

Published: March 17, 2023

Despite the fact that oral microecologics are effective in modulating gut microbiome, they always suffer from multiple insults during journey manufacture to arrival at intestine. Inspired by protective mechanism of mineralization, we describe a cytocompatible approach biointerface mineralization can generate an ultraresistant and self-removable coating on bacterial surface solve these challenges. Mineral endows bacteria with robust resistances against manufacture-associated oxygen exposure, ultraviolet irradiation, 75% ethanol. Following ingestion, is able actively neutralize gastric acid release encapsulated through spontaneous yet rapid double-decomposition reaction. In addition neutralization, generated calcium ions trigger micellar aggregation bile acid, enabling dual exemptions achieve uncompromised viability. Further supported therapeutic efficacy coated toward colitis mice, provides versatile platform for developing next-generation living biotherapeutics.

Language: Английский

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