Azithromycin-loaded PLGA Microspheres Coated with Silk Fibroin Ameliorate Inflammation and Promote Periodontal Tissue Regeneration DOI Creative Commons

Zhaoguang Ouyang,

Xiaoyu Chen,

Zhengyang Wang

et al.

Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 14, 2024

Abstract Periodontitis, a widespread inflammatory disease, is the major cause of tooth loss in adults. While mechanical periodontal therapy benefits disease treatment, adjunctive also necessary. Topically applied anti-inflammatory agents have gained considerable attention periodontitis therapy. Although azithromycin (AZM) possesses excellent properties, its bioavailability limited owing to poor water solubility and absence sustained release mechanisms. Herein, we synthesized biodegradable microspheres (AZM@PLGA-SF) for AZM locally ameliorate inflammation facilitate tissue regeneration. was encapsulated poly (lactic-co-glycolic acid) (PLGA) (AZM@PLGA) using single emulsion-solvent evaporation, followed by surface coating with silk fibroin (SF) via electrostatic adsorption, reducing initial burst AZM. In vivo, local treatment AZM@PLGA-SF significantly reduced restored healthy levels. Mechanically, formulated regulated microenvironment levels pro-inflammatory cytokines (tumor necrosis factor -α, interleukin [IL]-6, interferon-γ, IL-2, IL-17A) gingival crevicular fluid promoted expression (IL-4 IL-10). demonstrated biological safety. Therefore, introduce an substantial potential mitigating encouraging repair regeneration tissues.

Language: Английский

Harnessing Bacterial Membrane Components for Tumor Vaccines: Strategies and Perspectives DOI

Zhenxin Bai,

Xuanyu Wang,

Tianming Liang

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 27, 2024

Tumor vaccines stand at the vanguard of tumor immunotherapy, demonstrating significant potential and promise in recent years. While have achieved breakthroughs treatment cancer, they still encounter numerous challenges, including improving immunogenicity expanding scope vaccine application. As natural immune activators, bacterial components offer inherent advantages vaccines. Bacterial membrane components, with their safer profile, easy extraction, purification, engineering, along diverse array activate system improve efficacy. This review systematically summarizes mechanism action therapeutic effects membranes its derivatives (including vesicles hybrid biomaterials) Subsequently, authors delve into preparation based on biomaterials. Following this, outer are elucidated, mechanisms explained. Moreover, combination therapy analyzed. Last, challenges trends this field discussed. comprehensive analysis aims to a more informed reference scientific foundation for design implementation membrane-based

Language: Английский

Citations

4
Al-Based Metal–Organic Nanoslice-Coupled Phospholipid Layer Enables Highly Enriched Biological External Vesicles DOI

Bangming Wang,

Yan Zhao,

Mingzhu Zhao

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Systematic analysis of the information content biological external vesicles (BEVs) is essential for understanding complex relationships between metabolic processes and cells at a systemic level. Although considerable efforts have been made to enrich BEVs, their comprehensive in way that maintains stabilizes maintenance high doses remains challenge. To address this issue, we developed metal–organic nanoslice-coupled exocytosis phospholipid layer method utilized subtle interactions nanoslice molecular membrane achieve convenient efficient enrichment BEVs. The bypassed conventional ultracentrifugation size exclusion separation helped highly preserve internal on By integrating analyzing enriched BEVs elements such as proteins endotoxins, simplicity robustness vesicle surface technology were verified, which provided reliable platform studying cellular events associated with outer expanded applications nanoslice.

Language: Английский

Citations

0
New Advances in Periodontal Functional Materials Based on Antibacterial, Anti‐Inflammatory, and Tissue Regeneration Strategies DOI Open Access
Haoyue Wu, Yuanfeng Li, Linqi Shi

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

With the global population aging, awareness of oral health is rising. Periodontitis, a widespread bacterial infectious disease, gaining attention. Current novel biomaterials address key clinical issues like infection, gum inflammation, tooth loosening, and loss, focusing on antibacterial, anti-inflammatory, tissue regeneration properties. However, strategies that integrate advantages these to achieve synergistic therapeutic effects by clearing biofilms, inhibiting inflammation activation, restoring periodontal soft hard functions remain very limited. Recent studies highlight link between periodontitis systemic diseases, underscoring complexity disease. There an urgent need find comprehensive treatment plans requirements. Whether integrating new enhance existing treatments or developing approaches replace traditional therapies, efforts will drive advancements in treatment. Therefore, this review compares with treatments. It highlights design concepts mechanisms functional materials, their properties, discusses importance strategies. This aims provide guidance for emerging research promote development precise efficient

Language: Английский

Citations

0
Bacterial meningitis is a significant catalyst for neuroinflammation DOI
Sudharsan Parthasarathy, Siva Vijayakumar Tharumasivam,

Ashajyothi Chavaan

et al.

International review of neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Orally Administered ZnxCeyO2/Se Hydrogel with Effective Antioxidant Activity for Treating Inflammatory Bowel Disease by Inhibiting Ferroptosis DOI

Lingling Kan,

Tongsheng Li, Weinan Zhang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Oxidative stress leads to intestinal barrier damage, which induces immune responses occur and further promotes oxidative exacerbating inflammatory bowel disease (IBD). In this work, the multifunctional Zn x Ce y O 2 /Se (ZCSO) nanozyme wrapped with acid‐resistant calcium alginate hydrogel designed for oral administration is prepared. The ZCSO can promote activation of Nrf2 pathway, then significantly improve efficiency scavenging reactive oxygen species (ROS) up‐regulate protein expression glutathione peroxidase 4 (GPx4), closely related inhibition ferroptosis. addition, inhibiting growth some pathogenic bacteria proliferating due shows a positive regulation flora reduces secretion pro‐inflammatory factors levels macrophages, achieving significant preventive delayed therapeutic effect colitis mice. Consequently, distinctive properties render it promising candidate treatment IBD by effectively ROS, thereby interrupting detrimental cycle between response, ultimately promoting proliferation epithelial cells reestablish integrity mucosal barrier.

Language: Английский

Citations

0
Bioengineered bacterial extracellular vesicles for targeted delivery of an osteoclastogenesis-inhibitory peptide to alleviate osteoporosis DOI
Xiangxiang Kong, Han Liu,

Sumeng Chen

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113751 - 113751

Published: April 1, 2025

Language: Английский

Citations

0
Pathogenic mechanisms and potential applications of extracellular vesicles from periodontal pathogens in periodontitis DOI Creative Commons
Ruiqing Zhang, Guoliang Li,

Yingtao Wu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 20, 2024

Periodontitis is a multifactorial disease characterized by chronic destruction of the periodontal supporting tissues and closely associated with dysbiosis plaque biofilm. It leading cause tooth loss in adults. Bacterial extracellular vesicles (BEVs) are released from bacteria, which range size 20 to 400 nm. These contain various components derived their parent including nucleic acids, proteins, lipids, other molecules, facilitate functions such as molecular transfer, metabolic regulation, bacterial interactions, biofilm formation, immune modulation. BEVs participated pathophysiological process periodontitis. Recently emerging evidence also showed that contents EVs saliva gingival crevicular fluid (miRNAs, mRNAs, proteins) could be used potential biomarkers for While most current research focuses on human-derived components, much less known about BEVs. Therefore, this review introduces formation mechanisms related Then, summarizes information mechanism, diagnostic theraputic value pathogen-derived development Furthermore, future challenges exploring role periodontitis discussed.

Language: Английский

Citations

2
Clinical and Microbiological Periodontal Biofilm Evaluation of Patients with Type I Diabetes DOI Open Access

Mihaela Mariş,

Maria-Alexandra Mârțu, Marius Mariş

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(22), P. 6724 - 6724

Published: Nov. 8, 2024

The purpose of this study was to assess the microbial composition and density subgingival plaque samples for periodontal pathogens while correlating values with glycemic control levels via glycated hemoglobin (HbA1c), a type that has chemically linked glucose, in I diabetes individuals who will undergo complex oral rehabilitation through orthodontic treatment implant surgery.

Language: Английский

Citations

1
Biochar Reduced the Risks of Human Bacterial Pathogens in Soil via Disturbing Quorum Sensing Mediated by Persistent Free Radicals DOI

Yangjuan Cheng,

Kun Lu, Zaiming Chen

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Biochar has great potential in reducing the abundance of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) from soil. However, its efficiency removing other biological pollutants, such as human bacterial pathogens (HBPs) virulence factor (VFGs), is rarely studied. Herein, by pyrolyzing rice straw (RS) pine wood (PW) at 350 700 °C, we prepared a series biochar (RS350, RS700, PW350, PW700) investigated their impacts on pathogenicity HBPs. Compared with PW biochar, RS effectively reduced HBPs 6.3-40.1%, well pathogenicity, evidenced an 8.2-10.1% reduction VFGs. Mechanistically, more persistent free radicals (PFRs) were formed than that during pyrolysis, PFRs triggered degradation

Language: Английский

Citations

1
Azithromycin-loaded PLGA Microspheres Coated with Silk Fibroin Ameliorate Inflammation and Promote Periodontal Tissue Regeneration DOI Creative Commons

Zhaoguang Ouyang,

Xiaoyu Chen,

Zhengyang Wang

et al.

Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 14, 2024

Abstract Periodontitis, a widespread inflammatory disease, is the major cause of tooth loss in adults. While mechanical periodontal therapy benefits disease treatment, adjunctive also necessary. Topically applied anti-inflammatory agents have gained considerable attention periodontitis therapy. Although azithromycin (AZM) possesses excellent properties, its bioavailability limited owing to poor water solubility and absence sustained release mechanisms. Herein, we synthesized biodegradable microspheres (AZM@PLGA-SF) for AZM locally ameliorate inflammation facilitate tissue regeneration. was encapsulated poly (lactic-co-glycolic acid) (PLGA) (AZM@PLGA) using single emulsion-solvent evaporation, followed by surface coating with silk fibroin (SF) via electrostatic adsorption, reducing initial burst AZM. In vivo, local treatment AZM@PLGA-SF significantly reduced restored healthy levels. Mechanically, formulated regulated microenvironment levels pro-inflammatory cytokines (tumor necrosis factor -α, interleukin [IL]-6, interferon-γ, IL-2, IL-17A) gingival crevicular fluid promoted expression (IL-4 IL-10). demonstrated biological safety. Therefore, introduce an substantial potential mitigating encouraging repair regeneration tissues.

Language: Английский

Citations

1